• 생명과학회지
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• 제18권9호
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• pp.1212-1218
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• 2008

Conjugated linoleic acid (CLA)와 $\gamma$-oryzanol (OZ) 혼합물의 mouse 체지방 및 복부지방 감소에 관한 연구를 하였다. Female ICR mice (10주 령)을 몸무게 차이가 없도록 Control (70 ${\mu}l$ olive oil + 30 ${\mu}l$ CLA), CLA-OZ 1 (70 ${\mu}l$ olive oil + 30 ${\mu}l$ CLA + 0.5 mg OZ), CLA-OZ 2 (70 ${\mu}l$ olive oil + 30 ${\mu}l$ CLA + 1.0 mg OZ), OZ (100 ${\mu}l$ olive oil + 1.0 mg OZ) 및 Olive oil (100 ${\mu}l$ olive oil)로 구분하였다. 처리시료는 매일 4주간 경구투여 하였고 식이와 물은 자유롭게 먹게 하였다. 시료처리 4주 후에 mice의 몸무게를 달고 탈골법으로 sacrifice하여 전체몸무게, 복부지방 무게, 장기와 복부지방을 제거한 나머지 부분 무게 (empty carcass weight: ECW)를 측정하였다. CLA (control) 처리는 olive oil 처리에 비해 유의성 있게 몸무게, ECW, 복부지방 및 체지방을 감소시켜 CLA가 복부지방 및 체지방을 감소시킴을 알 수 있었다. CLA-OZ 1 처리는 OZ 처리에 비해서는 복부지방 및 체지방을 유의성 (p<0.05) 있게 감소시켰으나 CLA (control) 처리에 비해서는 유의성이 없었다. 또한 CLA-OZ 2 처리는 control 처리 및 OZ 처리에 비해 복부지방 및 체지방을 유의성 (p<0.05) 있게 감소시켰다. 이 결과는 CLA와 OZ를 혼합하여 처리한 mice에서 이들을 각각 단독으로 처리한 mice에서보다 복부지방 및 체지방이 많이 감소되어 OZ가 CLA의 지방감소 작용에 상승효과가 있음을 의미한다.

• Journal of Nutrition and Health
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• 제34권4호
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• pp.367-374
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• 2001

Conjugated linoleic acid(CLA) is a naturally occuring group of derivatives of linoleic acid found in beef and dairy products. CLA has reported to reduce body fat. This study was designed to observe the effect of CLA supplementation on fat accumulation and degradation in male Sprague Dawley rats. Seventy two rats, weighing 150-180g, were divided into 2 groups according to the types of dietary fat(beef tallow or fish oil) and then each group was divided into 2 groups depending on CLA supplementation, i.e., BT, BT-CLA, FO, FO-CLA. All rats were fed experimental diet containing total fat at 12%(w/w) including CLA at 1% for 30 weeks. At 30 weeks, rats were sacrificed to measure TG, free fatty acid level in plasma, TG, lipogenic enzymes in lever and fat cell size, LPL and HSL activities in epididymal fat fad. Fish oil supplemented with CLA diet showed significant reduction in the food efficiency and weight in 30 weeks-fed rats. CLA supplement did not effect on plasma TG, hepatic TG levels and lipogenic enzymes activities in rats, but, fish oil significantly reduced, The LPL and HSL activities did not affected by CLA supplement and n-3 fatty acid rich fish oil. In conclusion, the results suggest that CLA supplement was not a proper way to reduce the fat accumulation in Sprague Dawley rats. Fish oil supplemented with CLA might better way to reduce the body fatness than fish oil itself. Therefore, It is recommended that further study be performed related to physiological and biochemical effects of CLA supplementation and n-3 fatty acid in rats for the reduction of body fatness.(Korean J Nutrition 34(4) : 367∼374, 2001)

• 한국식품과학회지
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• 제31권6호
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• pp.1453-1457
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• 1999

항산화 및 항암 작용이 있는 것으로 알려진 conjugated linoleic acid(CLA)를 식물성 유지로부터 알칼리 이성질화에 의해 고 농도의 CLA 농축물을 얻기 위한 최적 조건을 연구하였다. 지방산 조성이 상이한 식물성 유지를 알칼리 이성질화 하였을 때 CLA 생성량은 유지중 linoleic acid 함량이 많은 것이 가장 높았다. 홍화유를 알칼리 이성질화할 때 KOH 농도는 $8{\sim}11%$, 가열온도는 $180{\sim]185^{\circ}C$에서 cis-9, trans-11 CLA와 총 CLA 생성량이 가장 높았고, 가열시간은 CLA 생성량에 큰 영향을 미치지는 않았으나 $20{\sim}40$분이 가장 적당하였다. 홍화유를 silicic acid column chromatograph)에 의해 중성, 당 및 인지방질의 lipid class를 분획하여 알칼리 이성질화를 시켰을 때 중성지방질의 획분에 CLA가 가장 많이 함유되었다. 알칼리 이성질화시킨 홍화유를 요소처리와 HPLC 분획에 의해 95.4%의 CLA 농축물을 얻을 수 있었다.

• Journal of Nutrition and Health
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• 제34권8호
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• pp.858-864
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• 2001

The study was designed to observe an antioxidant activities of conjugated linoleic acid(CLA) in rat liver by determining the activities of antioxidative enzymes(superoxide dismutase, glutathione peroxidase, catalase) and the levels of tocopherol and thiobarbituric acid reactive substance(TBARS). Male Sprague Dawley rats at weeks-old were divided into groups according to the types of dietary fat(beef tallow and fish oil) and then each group was subdivided into groups depending on CLA supplement. All rats were fed experimental diet containing 12% total fat including 1% CLA by weight for 30 weeks. CLA supplemented to beef tallow diet did not have significant effect on the level of TBARS and tocopherol. The level of TBARS was significantly increased in fish oil diet(highly unsaturated fat diet), but its level was significantly reduced by increasing SOD and GSH-Px activities when CLA was supplemented to fish oil diet so that CLA showed a sparing action of tocopherol in tissue. CLA did not have significant effect on peroxisomal catalase activities, but its activity was significantly increased when TBARS production was high in the fish oil diet. CLA could be incorporated into phospholipid of microsomal membrane, and interfered the conversions of C18 : 0 into C18 : 1 and C18 : 2 into C20 : 4 in liver. In conclusion, CLA had an antioxidant activities depending on the type of fat in diet. Therefore, it could be recommended to use CLA when highly unsaturated fat was used in meal preparation.

• 농업과학연구
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• 제41권1호
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• pp.47-58
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• 2014

The effect of conjugated linoleic acid (CLA) isomers esterified in diacylglycerol (DAG)-rich oil on lipid metabolism was investigated. Since dietary DAG has been known to induce the regression of atherosclerosis, CLA-DAG and olive-DAG oils containing similar levels of DAG (51.4~54.2%) were synthesized from olive oil. Hyperlipidemic C57BL/6J mice were then fed high-fat high-cholesterol diets supplemented with these oils (5% each) for 7 wk. The CLA-DAG diet containing 2.1% CLA isomers (0.78% c9,t11-CLA; 1.18% t10,c12-CLA) remarkably increased the levels of total plasma cholesterol and glutamic oxaloacetic transaminase (GOT) along with hepatic cholesterol and triacylglycerol (TAG) contents. Furthermore, the CLA-DAG diet inhibited fat uptake into adipose tissue whereas fat deposition (especially in the liver) was increased, resulting in the development of fatty livers. Hepatic fatty acid composition in the CLA-DAG mice was different from that of the olive-DAG mice, showing higher ratios of C16:1/C16:0 and C18:1/C18:0 in the liver. The activity of hepatic acyl-CoA:cholesterol acyltransferase (ACAT) was higher in CLA-DAG mice while plasma lecithin:cholesterol acyltransferase (LCAT) activity and the ferric reducing ability of plasma (FRAP) were lower in CLA-DAG mice compared to the olive-DAG animals. Results of the present study suggest that CLA incorporation into DAG oil could induce atherosclerosis in mice.

• Preventive Nutrition and Food Science
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• 제6권1호
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• pp.19-22
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• 2001

Conjugated linoleic acid (CLA) exhibits potent anti-carcinogenic and other biological activities in several animal models. We report here that dietary CLA, chemically synthesized from corn oil, accumulates I silkworm, Bombyx mori, which is used as therapeutic agent for diabetes in Korea and Japan. Mulberry leaves treated with 0.1 or 10% CLA in ethanol were supplied to silkworms from the end of 3rd instar to the 3rd day of the 5th instar. Fresh mulberry leaves or leaves treated with 10% corn oil in ethanol were fed as a check treatment. the amount of total lipids in the larval body ranged from 17.4 to 19.1mg/g of body tissue, which was not significantly affected by the source of the diets. No CLA was found in the control silkworms. But the level of CLA significantly increased to 83.5 mg/g of fat, when fed with mulberry leaves treated with 10% CLA. Only trace amounts of CLA were detected in the larvae reared with check leaves and 0.1% CLA-treated leaves. Mulberry leaves treatd with corn oil or CLA were not palatable to the larvae, resulting in a reduction of larval weight. These results suggest that silkworms containing CLA in body lipids could be producted by dietary CLA.

• Asian-Australasian Journal of Animal Sciences
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• 제18권2호
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• pp.221-225
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• 2005

An in vitro study was conducted to examine the effect of monensin or fish oil addition on bio-hydrogenation of $C_{18^-} unsaturated fatty acids and CLA production by mixed ruminal bacteria when incubated with safflower oil. Commercially manufactured concentrate (1%, w/v) with safflower oil (0.2%, w/v) were added to mixed solution (600 ml) of strained rumen fluid and McDougalls artificial saliva (control). Monensin $Rumensin^{(R)}$, 10 ppm, w/v, MO), mixed fish oil (0.02%, w/v, absorbed to 0.2 g alfalfa hay, FO) or similar amounts of monensin and fish oil (MO+FO) to MO and FO was also added into the control solution. All the culture solutions prepared were incubated in the culture jar anaerobically at $39^{\circ}C$ up to 12 h. Higher pH (p<0.047) and ammonia concentration (p<0.042) were observed from the culture solution containing MO at 12 h incubation than those from the culture solutions of control or FO. The MO supplementation increased (p<0.0001-0.007) propionate proportion of culture solution but reduced butyrate proportion at 6 h (p<0.018) and 12 h (p<0.001) of incubations. Supplementation of MO or MO+FO increased (p<0.001) the proportions of $C_{18:2}$. The MO alone reduced (p<0.022-0.025) the proportion of c9,t11-CLA compared to FO in all incubation times. The FO supplementation increased the proportion of c9,t11-CLA. An additive effect of MO to FO in the production of c9,t11-CLA was observed at 6 h incubation. In vitro supplementation of monensin reduced hydrogenation of $C_{18^-}$UFAs while fish oil supplementation increased the production of CLA.

• 한국식품조리과학회지
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• 제14권1호
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• pp.84-90
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• 1998

Conjugated linoleic acid(CLA)의 생성과 식품중의 함량 및 유지에 대한 항산화효과를 알아보기 위하여 methyl linoleate(LA)와 대두유(SBO)를 20$\pm$1$^{\circ}C$, 40$\pm$1$^{\circ}C$에서 저장하면서 그의 생성량을 측정하였고 상용되고 있는 시판우유 6종류와 쇠고기, 돼지고기, 닭고기, 그리고 청어, 고등어, 삼치, 꽁치 중의 CLA함량을 측정하였다. 또한 CLA, BHT, tocopherol을 기질 대두유에 첨가하고 40$\pm$ 1$^{\circ}C$에서 저장하고, 상승제로서 citric acid를 같이 첨가하여 60$\pm$1$^{\circ}C$에서 저장하면서 POV를 측정하여 항산화효과를 비교하였으며 또한 이들을 180$\pm$1$^{\circ}C$에서 가열하면서 그의 효과를 동시에 측정한 결과는 다음과 같았다. 1. LA에서의 CLA의 생성은 20$\pm$1$^{\circ}C$에서 저장한 경우, 28일째에 초기량(188ppm)의 약 14배 이상으로 증가되었으며 40$\pm$1$^{\circ}C$에서는 14일까지 생성량이 증가되나 그 이후는 감소되었다. 대두유에서도 20$\pm$1$^{\circ}C$인 때 14일까지 생성량이 높았으며 40$\pm$1$^{\circ}C$인 때는 7일까지 생성량이 증가되나 그 이후 약간씩 감소되어 LA 및 SBO 모두 20$\pm$1$^{\circ}C$인 때에 CLA의 생성 이 좋은 것으로 나타났다. 2. 식물성 기름 중에서 CLA함량은 유채유에 348ppm으로 가장 높았으며 40$\pm$1$^{\circ}C$에서 저장시 면실유의 경우 초기에 292ppm이던 것이 저장 28일째에 1322 ppm으로 증가되고 또한 POV도 10.05 meq/kg oil로 낮아 CLA의 생성이 매우 유효하였다. 대두유는 저장 7일에 767 ppm으로 상당히 큰 증가량을 보인 반면 유채유는 대체적으로 저장시 에 감소되었다. 3. 시판 우유중의 CLA함량은 293~2148 ppm이었으며 제조회사에 따라 크게 차이가 있었고 육류중에서는 돼지고기에 2370ppm으로 높았으며 생선 중에서는 삼치에 1040 ppm으로 높았으며, 삼치를 조림했을 경우에는 2039 ppm으로 증가되었다. 4. 저온 저장시 CLA의 유지에 대한 항산화효과는 대두유에서는 초기 7일에는 tocopherol 보다는 크고 BHT와 거의 유사하였으며, 21일 이후에는 항산화효과가 없고 오히려 산화를 촉진시키는 것으로 나타났다. 또한 CLA의 첨가량이 많을수록 항산화효과는 떨어졌으나 상승제인 citric acid가 공존하면 항산화효과가 BHT나 tocopherol보다 더 높게 나타났다. 옥수수유에서 CLA의 항산화효과는 저장 중 BHT나 tocopherol 보다 항산화효과가 높게 나타나 기질유지의 종류에 따라 차이가 있었다 180$\pm$1$^{\circ}C$에서 유지를 가열하는 경우에는 CLA의 항산화효과가 크게 나타났으며 그 정도는 BHT나 tocopherol과 거의 유사하였고 저온 저장시의 경우 보다 더 높은 항산화효과를 보였다

• Journal of Animal Science and Technology
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• 제48권4호
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• pp.521-532
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• 2006

본 실험은 대두유 또는 아마유를 in vitro 방법으로 배양 할 때, 탄수화물원의 첨가수준이 반추위 박테리아에 의한 bio-hydrogenation과 CLA 생성에 미치는 효과를 조사하였다. 4수준(0%, 0.3%, 0.6% 그리고 0.9%, w/v)의 혼합된 탄수화물원(glucose, cellobiose, soluble starch, 1:1:1, w/w/w)과 두 종류의 oil을 cellulose powder에 흡착시킨 형태로 각각 60mg씩 인공타액(120ml)과 반추위액(30ml)이 혼합된 배양액(150ml)에 넣은 다음 39℃에서 12시간동안 혐기적으로 배양하였다. 배양액의 pH와 암모니아 농도는 두 종류 oil을 첨가한 배양액 모두에서 탄수화물원의 첨가 수준이 높을수록 pH와 암모니아 농도가 낮았다(P<0.05). 탄수화물원의 첨가 수준이 증가할수록 total VFA 생성량 역시 증가되었으나(P<0.01) 첨가한 oil 간의 차이는 없었다. 배양시간이 경과됨에 따라 탄수화물원의 첨가수준이 높을수록 propionate의 조성비율이 증가된 반면(P<0.001) acetate와 butyrate의 조성비율은 감소되었다. 배양 후 3시간이 경과하였을 때 배양액 내 oleic acid의 조성비율은 대두유에 비하여 아마유를 첨가한 배양액에서 낮았으나(P<0.001) linoleic acid의 비율은 높았다(P<0.001). 이와는 달리 탄수화물원의 수준이 증가될수록 stearic acid(P<0.05), CLA(P<0.01) 및 cis-9, trans-11 CLA(P<0.001)의 조성비율은 감소되었으나, linoleic acid의 조성 비율은 증가되었다(P<0.05). Linolenic acid의 조성비율에 있어서는 첨가된 oil의 종류와 첨가된 탄수화물원의 수준간의 상호작용이 있는 것으로 나타났는데(P<0.001), 12시간의 배양종료 후 대두유 첨가구에 비해 아마유 첨가구에서 stearic acid(P<0.01), oleic acid(P<0.001), 그리고 trans-11C18:1(P<0.01)의 조성비율이 감소된 반면, linoleic acid(P<0.001)와 linolenic acid(P<0.01)의 조성비율은 증가되었다. 탄수화물원의 첨가수준이 증가될수록 stearic acid와 총 CLA의 조성비는 감소되었으나(P<0.01), trans-11-C18:1(P<0.05)와 linoleic acid(P<0.01)의 조성비율은 증가되었다. 배양 12시간 후 배양액 내의 oleic acid (P<0.05), linoleic acid(P<0.05) 및 linolenic acid(P<0.01)의 조성비율에 있어서는 첨가한 oil의 종류와 첨가한 탄수화물원의 수준간의 상호작용이 있었는데, 탄수화물원의 첨가수준이 감소됨에 따라 cis-9, trans-11 CLA와 trans-10, cis-12 CLA의 조성비 역시 감소되는 경향이었으나 첨가한 oil의 종류에 대한 영향은 거의 받지 않았다. 본 실험의 결과를 종합해 볼 때, 탄수화물의 첨가수준과 oil의 첨가는 반추미생물의 bio-hydrogenation 작용 및 CLA 생성에 영향할 수 있는 것으로 여겨진다.

• Asian-Australasian Journal of Animal Sciences
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• 제24권9호
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• pp.1249-1255
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• 2011

This study examined the effect of CLA, flaxseed oil and fish oil and their combination forms on crude fat of liver and fatty acid profiles of liver, breast and thigh meat in broiler chicks. A total of 72, 1-day-old Cobb broilers were assigned to 6 groups, and fed an experimental diet supplemented with 5 different fat sources; conjugated linoleic acid (2% CLA), flaxseed oil (2% FXO), fish oil (2% FHO), CLA and flaxseed oil combination (1:1; 2% CXO), and CLA and fish oil combination (1:1; 2% CHO). Eight birds per treatment were processed, and liver, breast and thigh samples were investigated at 21 d of age. As a result of this study, most fatty acids of liver, breast and thigh meat were influenced by fat sources supplemented in the diet (p<0.05). CLA addition resulted in an increase of crude fat and saturated fatty acid (SFA) content but a concomitant decrease in n-3 to n-6 fatty acid ratio was observed in liver (p<0.05). Moreover, the same trends of SFA and n-3 to n-6 fatty acid ratio were also observed in breast and thigh meats of birds fed CLA alone. In the CXO-fed group or CHO-fed group, n-3 and n-3 to n-6 fatty acid ratio in both breast and thigh meat increased compared with CLA group, while SFA content decreased (p<0.05). FHO fed-groups had the lowest proportion of n-6 fatty acid in both breast and thigh meats compared to other fat source treatments (p<0.05). In conclusion, the increased levels of crude fat and SFA in liver and meats obtained by feeding CLA could be reduced by its combination with FXO or FHO. In addition, the combination of CLA and FXO or FHO fed to broiler chicks could increase the n-3 to n-6 fatty acid ratio of their meat along with the deposition of CLA.