• Journal of Chest Surgery
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• 제37권9호
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• pp.762-767
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• 2004

최근 비침습적인 수술방법이 발전하면서 체외순환 없이 시행하는 관상동맥우회로술에 대한 관심이 증가하게 되었으며 체외순환의 여러가지 부작용들이 발표되면서 비체외순환하의 수술이 점차로 보편화되어 본원에서 시행한 비체외순환 및 체외순환하에서의 관상동맥우회로술의 성적을 비교 분석하였다. 대상 및 방법: 2001년 1월부터 2003년 6월까지 관상동맥우회로술을 시행 받았던 87명의 환자를 대상으로 하였다. 체외순환하 관상동맥우회로술 36예, 비체외순환하 관상동맥우회로술 51예를 시행하였으며 양군에서 수술 전 위험인자와 관상동맥 질환정도, 평균수술시간, 술 후 삽관기간, 평균 중환자실 재원기간, 평균 재원기간, 술 후 강심제의 사용, 술 후 출혈량, 술 후 심근효소치를 비교 분석하였다. 결과: 두 군 의 남녀비, 연령, 술 전 위험요소에 대한 통계적 유의성은 없었으며 심근경색 기왕력, 흉통의 정도(Canadian Class), 관상동맥 질환의 정도 및 심박출 계수도 통계적인 차이를 보이지 않았다. 평균 수술시간은 체외순환하 수술군에서 372 $\pm$ 142.2분, 비체외순환하 수술군에서 270 $\pm$ 79.3분으로 비체외순환하 수술군에서 유의한 차이를 보였다(p < 0.001). 이식편수는 체외순환 수술군이 2.2$\pm$0.5개, 비체외순환 수술군이 1.7$\pm$0.7개로 체외순환 수술군이 유의하게 많았다(p<0.001). 술 후 삽관기간은 체외순환하 수술군에서 24.3 $\pm$ 17.8 시간, 비체외순환하 수술군에서 17.1 $\pm$ 13.1 시간으로 비체외순환하 수술군에서 유의하게 짧았다(p < 0.05). 술 후 CK-MB는 비체외순환하 수술군에서 8.9$\pm$ 18.7 U/L로 체외순환하 수술군의 25.7 $\pm$8.4 U/L로 유의하게 낮았다(p<0.001). 중환자실 평균 재원기간은 체외순환하 수술군에서 21.3 $\pm$ 21.3시간, 비체외순환하 수술군에서 15.6 $\pm$ 7.6시간으로 비체외 순환하 수술군에서 낮았다(p<0.05). 술 후 대동맥내 풍선 펌프의 삽입은 체외순환하 수술군에서 2예가 있었고 체외순환하 수술군에서 8예로 유의하게 많았다(p<0.05). 술 후 강심제의 사용은 체외순환하 수술군에서 21예, 비체외순환하 수술군이 9예로 체외순환하 수술군에서 유의하게 높았다(p< 0.001). 술 후 부정맥은 비체외순환하 수술군이 3예, 체외순환하 수술군이 9예로 체외순환하 수술군에서 발생빈도가 유의하게 높았다(p<0.05). 술 후 사망률은 비체외순환하 수술군에서 2명(3.9%)이였고 체외순환하 수술군에서 3명(8.3%)으로 통계적으로 유의하지 않았다. 그 외에 술 후 24시간 출혈량, 출혈로 인한 재수술, 평균 재원일수, 술 후 감염, 신부전, 신경학적 합병증 등이 수술 후 결과와 유병률에는 두 군간에 통계적 유의성은 없었다. 결론: 비체외순환하 수술군에서 평균 수술시간, 술 후 기관 삽관기간, 평균 중환자실 재원기간이 짧고 술 후 심근 손상의 정도는 적으나 체외순환하 수술군에 비해 이식편수가 적었다. 비체외순환하 관상동맥우회로술이 체외순환하 우회로술에 비해 결과에 있어 몇 가지 장점을 보여 주고 있으나 향후 더 많은 임상경험의 축적 및 장기 추적 관찰이 필요하리라 생각한다.

• Tuberculosis and Respiratory Diseases
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• 제47권4호
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• pp.478-487
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• 1999

연구배경 : 전자현미경적 관찰에 의해 진단된 흉막의 악성중피종과 전이성선암 환자를 대상으로 하여 7종의 단일클론항체를 이용한 면역조직화학염색을 시행하고 이들의 발현양상을 비교하여 두 질환을 감별할 수 있는 가장 효과적인 염색방법을 알아보고자 하였다. 방 법 : 면역조직화학염색법은 Fisher사(Fisher scientific)의 1-hour immunohistochemistry방법을 이용하였다. 종양조직은 포르말린으로 고정된 파라핀 조직을 이용하여, $3{\mu}m$ 두께로 잘라서, $85^{\circ}C$에서 가열시킨 후 파라핀을 제거하고, 희석액과 단클론항체를 $45^{\circ}C$에서 15분간 반응시켰다. 각 단계마다 automation buffer로 실온에서 세척하였고, avidin-biotin-peroxidase system을 이용한 immunoperoxidase method로 염색하였다. 조직절편은 $45^{\circ}C$에서 10분간 색소원반응을 시행하였고, 대조염색은 hematoxylin으로 실시하였다. 조직 절편은 최소한 각기 다른 두 구역에서 세포질 또는 세포막이 분명하게 염색될 때 양성으로 판정하였다. 결 과 : 7종의 단일클론항체중 CK, EMA, vimentin, S-100 단백과 Leu-M1은 악성중피종과 전이성선암에서 발현율의 차이는 없었으나, B72-3은 전이성선암에서만 발현되었고, CEA는 전이성선암 전례와 악성중피종 42.9%에서 발현되어서 민감도는 높았으나 특이도는 낮았다. 결 론 : B72-3을 이용한 면역조직화학염색은 악성중피종과 전이성선암의 감별진단에 가장 유용한 방법으로 시사되었으며, 기존에 사용되고 있는 CEA와 B72-3을 함께 이용한다면 악성중피종과 전이성선암에 대한 감별진단력을 높일 수 있을 것으로 사료된다.

• 한국발생생물학회지:발생과생식
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• 제15권4호
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• pp.339-347
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• 2011

Human eyelid adipose-derived stem cells (hEAs) and amniotic mesenchymal stem cells (hAMs) are very valuable sources for the cell therapeutics. Both types of cells have a great proliferating ability in vitro and a multipotency to differentiate into adipocytes, osteoblasts and chondrocytes. In the present study, we evaluated their stem cell characteristics after long-time cryopreservation for 6, 12 and 24 months. When frozen-thawed cells were cultivated in vitro, their cumulative cell number and doubling time were similar to freshly prepared cells. Also they expressed stem cell-related genes of SCF, NANOG, OCT4, and TERT, ectoderm-related genes of NCAM and FGF5, mesoderm/endoderm-related genes of CK18 and VIM, and immune-related genes of HLA-ABC and ${\beta}$2M. Following differentiation culture in appropriate culture media for 2-3 weeks, both types of cells exhibited well differentiation into adipocyte, osteoblast, and chondrocyte, as revealed by adipogenic, osteogenic or chondrogenic-specific staining and related genes, respectively. In conclusion, even after long-term storage hEAs and hAMs could maintain their stem cell characteristics, suggesting that they might be suitable for clinical application based on stem cell therapy.

• 대한수의학회지
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• 제43권3호
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• pp.507-516
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• 2003

This study was performed to clarify the induction possibility of anaphylactoid reactions by the administration with the heartworm extracts, and, if any, to elucidate different virulences in terms of the protein concentrdtions and sexes of Dirofilaria immitis. Twenty three clinically healthy D. immitis-free adult dogs were used in the present study. The experimental animals were divided into 5 groups. Group A (5 heads) was administered with an female heartworm extract containing 0.1 g/dl protein concentration. Group B (4 heads) was administered with an male heartworm extract containing 0.1g/dl protein concentration. Group C (5 heads) was administered with an female heartworm extract containing 0.2 g/dl protein concentration. Group D (4 heads) was administered with an male heartworm extract containing 0.2 g/dl protein concentration. Group E (5 heads) was administered with an female heartworm extract containing 0.4 g/dl protein concentration. The changes of clinical symptoms and vital signs (body temperature, heart rate and respiration rate) were examined before and 5 minutes, 15 minutes, 30 minutes, 1 hour, 2 hours and 24 hours after injection with the extracts, respectively. In addition, the changes of hematological values (RBC, PCV and total leukocytes counts), serum chemical values (ALP and CK) were determined. It was considered that heartworm extract could induce anaphylactoid reaction and adult female heartworm extract was more affective than those of adult male heartworm extract in the changes of clinical symptoms, vital signs, hematological values and serum chemical values.

• Journal of Applied Biological Chemistry
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• 제57권1호
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• pp.41-45
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• 2014

In the present study, red ginseng extracts were fermented by Paecilomyces tenuipes and the protopanaxdiol-type ginsenosides in the extracts were bio-transformed to F2, Rg3, Rg5, Rk1, Rh2, and CK determined by a high-pressure liquid chromatography analysis. It indicates that P. tenuipes is a microorganism to biotransform protopanaxdiol-type ginsenosides to their less glucosidic metabolites. Other biotransformed metabolites during fermentation were also analyzed using a GC-MS and identified as 2-methyl-benzaldehyde, 4-vinyl-2-methylphenol, palmitic acid, and linoleic acid. Antiulcerogenic activity of the fermented red ginseng extract (FRGE) on gastric mucosal damage induced by 0.15 M HCl in ethanol in rats was evaluated. FRGE was shown to have a potent protective effect on gastritis with 60.5% of inhibition rate at the dose of 40 mg/kg when compared to 54.5% of the inhibition rate at the same dose for stillen, the currently used medicine for treating gastritis. Linoleic acid showed a strong inhibition on gastritis with 79.3% of inhibition rate at the dose of 40.0 mg/kg. FRGE exhibited a distinct anticancer activity including growth inhibition of the two human colon cancer cells HT29 and HCT116. HT29 cells were less susceptible to FRGE in comparison with HCT116 cells. Taken together, fungal fermentation of the red ginseng extract induced hydrolysis of some ginsenosides and FRGE exhibited potent antiulcerogenic and anticancer activities. These results refer to use FRGE as a new source for treating human diseases.

• 대한의생명과학회지
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• 제13권3호
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• pp.223-230
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• 2007

Low power laser and neuromuscular electrical stimulation have been utilized in many clinical applications for the treatment of musculoskeletal lesion. This study was to investigate the effects of low power He-Ne IR laser and neuromuscular electrical stimulation on the change of the serum biochemical components in experimentally induced muscle injured rats. The twenty Sprague-Dawley male rats were randomly assigned to the 4 groups: the normal group (n=5), the control group (n=5), the low power laser irradiation group (LLI) (n=5) and the neuromuscular electrical stimulation group (NMES) (n=5). The experimentally induced muscle injury was induced by the subcutaneous injection of a 0.1 ml Freund's Complete Adjuvant into the right hind paw and calf muscles in control, LLI and NMES groups. The LLI group was irradiated with He-Ne IR laser for 5 minutes every day during 10 days. Also, the NMES group was electrically stimulated 15 minutes every day during 10 days. The normal and control groups were not received with any therapeutic intervention. The authors performed tests which were the concentration of the serum biochemical components to detect the effects of therapeutic interventions. The results were as follows: 1. The level of the aspartate aminotransferase (AST) was significantly decreased in the LLI and NMES groups compare to the control group. 2. The level of the serum lactate dehydrogenase (LDH) was significantly decreased in the LLI and NMES groups compare to the control group. 3. The level of the serum creatine kinase (CK) was significantly decreased in the LLI and NMES groups compare to the control group. From the results, we could come to the conclusion that low power laser and neuromuscular electrical stimulation could be accelerating healing processes of skeletal muscle injury and further clinical work will be required.

• Asian-Australasian Journal of Animal Sciences
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• 제29권4호
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• pp.457-463
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• 2016

The goal of this work was to investigate the correlations between MyHC mRNA transcription and their corresponding protein expressions in porcine longissimus muscle (LM) during postnatal growth of pigs. Five DLY ($Duroc{\times}Landrace{\times}Yorkshire$) crossbred pigs were selected, slaughtered and sampled at postnatal 7, 30, 60, 120, and 180 days, respectively. Each muscle was subjected to quantity MyHCs protein contents through an indirect enzyme-linked immunosorbent assay (ELISA), to quantity myosin heavy-chains (MyHCs) mRNA abundances using real-time polymerase chain reaction. We calculated the proportion (%) of each MyHC to total of four MyHC for two levels, respectively. Moreover, the activities of several key energy metabolism enzymes were determined in LM. The result showed that mRNA transcription and protein expression of MyHC I, IIa, IIx and IIb in LM all presented some obvious changes with postnatal aging of pigs, especially at the early stage after birth, and their mRNA transcriptions were easy to be influenced than their protein expressions. The relative proportion of each MyHC mRNA was significantly positively related to that of its corresponding protein (p<0.01), and MyHC I mRNA proportion was positively correlated with creatine kinase (CK), succinate dehydrogenase (SDH), malate dehydrogenase (MDH) activities (p<0.05). These data suggested that MyHC mRNA transcription can be used to reflect MyHC expression, metabolism property and adaptive plasticity of porcine skeletal muscles, and MyHC mRNA composition could be a molecular index reflecting muscle fiber type characteristics.

• Journal of Ginseng Research
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• 제28권1호
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• pp.18-26
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• 2004

The effect of Panax ginseng administration in muscle inflammatory process induced after eccentric exercise, that causes myofibrillar disruption, was studied. Changes in lipid peroxidation, inflammation, glycogen levels in muscle and release of myocellular proteins to blood were measured. The analyses were performed immediately after eccentric exercise and over week since this period are necessary for the muscle damage-repair cycle. The ginseng extract (100 mg kg$^{-1}$ ) was orally administered to rats for three months, before the eccentric exercise performance. The results showed the protective role of ginseng against skeletal muscle damage. This effect could be associated with their membrane stabilising capacity since creatine kinase (CK) activity was significantly decreased 96 h post-exercise from 523$\pm$70 to 381$\pm$53 and 120 h post-exercise from 443$\pm$85 to 327$\pm$75 in treated animals. $\beta$-glucuronidase activity, as indicator of inflammation, showed a significant reduction of about 15-25% in soleus, vastus and triceps in these post-exercise times. The lipid peroxidation, measured by malondyaldehyde levels, was significantly decreased in the 24 h post-exercise period in soleus and vastus intermedius muscles and on the recovery period. Finally ginseng administration reduced significantly the decrease of the glycogen levels immediately after exercise and when the regenerative process took place (72-168 h post exercise). Collectively, the results have showed that ginseng did not inhibit the vital inflammatory response process associated with the muscle damage-repair cycle but presumably ameliorate the injury.

• 대한방사선치료학회지
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• 제10권1호
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• pp.88-93
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• 1998

Multileaf collimator is essential equipment in conformal radiation therapy, however the use is limitted by increase of penumbra width and undulating dose distribution at the field edge. The purpose of this study is to improve the penumbra and dose distribution in the multileaf collimator field edge. Measurement were performed with X-omat V film in solid water phantom using 6MV photon beam from Siemens linear accelerator. All the measurement were made along the central axis of $5{\times}5cm,\;10{\times}10cm$ circular field for constant SSD of 100 cm. To improve the penumbra and dose distribution collimator was rotated by 15 degrees from 0 to 90 degrees (collimator rotation method) and center was shifted to the longitudinal direction by fourth of lead width (center shift method). We compare the penumbra and dose distribution at the field edge to alloy block. Dose distribution and penumbra width at the feild edge of MLC showed undulated dose pattern and increased penumbra compared with alloy block. However, in the collimator rotation method and center shift method we abtained simular results with alloy block. Through the study we expected that clinical use of MLC will be increase.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2002년도 학술대회지
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• pp.159-175
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• 2002

The effect of Panax ginseng administration in muscle inflammatory process induced after eccentric exercise, that causes myofibrillar disruption, was studied. Changes in lipid peroxidation, inflammation, glycogen levels in muscle and release of myocellular proteins to blood were measured. The analyses were performed immediately after eccentric exercise and over week since this period are necessary for the muscle damage-repair cycle. The ginseng extract $(100\;mg\;kg^{-1})$ was orally administered to rats for three months, before the eccentric exercise performance. The results showed the protective role of ginseng against skeletal muscle damage. This effect could be associated with their membrane stabilising capacity since creatine kinase (CK) activity was significantly decreased 96 h post-exercise from $523{\pm}70\;to\;381{\pm}53$ and 120 h post-exercise from $443{\pm}85\;to\;327{\pm}75$ in treated animals. ${\beta}-glucuronidase$ activity, as indicator of inflammation, showed a significant reduction of about $15-25\%$ in soleus, vastus and triceps in these post-exercise times. The lipid peroxidation, measured by malondyaldehyde levels, was significantly decreased in the 24 h postexercise period in soleus and vastus intermedius muscles and on the recovery period. Finally ginseng administration reduced significantly the decrease of the glycogen levels immediately after exercise and when the regenerative process took place (72-168 h post exercise). Collectively, the results have showed that ginseng did not inhibit the vital inflammatory response process associated with the muscle damage-repair cycle but presumably ameliorate the injury.