• 한국디지털건축인테리어학회논문집
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• 제9권1호
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• pp.5-10
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• 2009

Recently, the public offices are going to be an important role of CIP(City Identity Program) and be a center of citizen's community activities. So, the aspect of the universal design (UD), the space performance assessment for a disable user is invaluable to designing the public office spaces. Purpose of this paper is to provide basic data for better design of public office space based on UD(universal design) principle. Conclusion based on the finding are as follows; First, checklist of environmental assessment for public space was composed of 7 basic principles of UD and 3 additional principles(aesthetic appreciation, maintenance and eco-design). Second, as the result of assessment, accessible and inclusive design is more necessary. Third, local characteristics should be developed and managed deliberately as to public space design. As the result of that, public office space design should an important part of reducing the isolation that was caused by physical and social disability. From now on, in public office space design, the integrated and continuous design program that is based on UD principle and eco-design will be necessary.

• 한국환경과학회지
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• 제11권4호
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• pp.271-279
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• 2002

The purpose of this study is to focus on the townscape of Daegu based on the urban characteristics of the landscape cognition and images captured by citizens. The analysis was performed by the data obtained from questionnaires and interviews. This study methods were deals the cognition characteristics, landmark landscape, visual preference landscape, image and satisfaction. The results are summarized as follows: 1. The orders of cognition landscapes were estimated Apple＞ Weather＞ Texture＞ Mt.Palgong＞ Daegu Tower＞ Pretty Girl＞ Mt. Apsan＞ Dalsung Park＞ Conservative＞ Dongsung Road, etc. That is constructed Nonphysical elements(62.0%) and Physical elements(38.0%) 2. The orders of representative landscape(Landmark) in city were estimated Mt.Palgong＞ Daegu Tower＞ Gat Rock＞ Mt.Apsan＞ Dalsung Park, etc. As a whole middle and old people(over 30gen) preferenced as a history landscape or natural landscape, but youth people(10-20gen) preferenced as visible and interesting artifical places. 3. While the positive attitudes for the image of city were traditional(3.30), intimacy(3.58), and rest(2.90), the negative attitudes were unnewly(2.34), closing(2.37) and narrow(2.40). Also total satisfaction for that was estimated 5.51. 4. Psychological factors, related to the satisfaction of the image of city were composed of four factors, individuality character, pleasure character, amenity character, formation character. And the presumption formula of satisfaction was: Satisfaction = 5.477 ＋ 0.752(Individuality) ＋ 0.470(Pleasure) ＋ 0.413(Amenity) ＋ 0.241(Formation).

• International Journal of Oral Biology
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• 제41권3호
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• pp.113-118
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• 2016

An FDA approved drug for the treatment of type II diabetes, Troglitazone (TRO), a peroxisome proliferator-activated receptor gamma agonist, is withdrawn due to severe idiosyncratic hepatotoxicity. In the search for new applications of TRO, we investigated the cellular effects of TRO on YD15 tongue carcinoma cells. TRO suppressed the growth of YD15 cells in the MTT assay. The inhibition of cell growth was accompanied by the induction of cell cycle arrest at $G_0/G_1$ and apoptosis, which are confirmed by flow cytometry and western blotting. TRO also suppressed the expression of cell cycle proteins such as cyclin D1, cdk2, cdk4, cyclin B1, cdk1(or cdc2), cyclin E1 and cyclin A. The inhibition of cell cycle proteins was coincident with the up-regulation of $p21^{CIP1/WAF1}$ and $p27^{KIP1}$. In addition, TRO induces the activation of caspase-3 and caspase-7, as well as the cleavage of PARP. Further, TRO suppressed the expressions of Bcl-2 without affecting the expressions of Bad and Bax. Overall, our data supports that TRO induces cell cycle arrest and apoptosis on YD15 cells.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2004년도 제22회 춘계학술대회논문집
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• pp.687-692
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• 2004

For the prediction of sloshing in the propellant tank of rocket vehicle utilized in RVT (reusable rocket vehicle testing) conducted by ISAS/JAXA, the flow field in the propellant tank during the ballistic flight was experimentally reproduced with the sub-scale model of it. The lateral acceleration as large as about 0.8 G was provided with a mechanical exciter and the deformation of liquid surface in the vessel was visualized with a high-speed camera. The several con-figurations of damping devices were installed and tested in the vessel, which should keep the ullage gas away from the outlet port. It was consequently suggested that the combination of a baffle plate and a perforated cylinder could be effective against the gas suction before the re-ignition of the engine. The sloshing phenomena were also simulated with the CFD code, called CIP-LSM. The numerical results showed good agreement with the corresponding data obtained in the experiment.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2021년도 춘계학술발표대회
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• pp.256-258
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• 2021

최근 전세계 각 연구기관에서 CPS, 클라우드 컴퓨팅, 5G, 빅데이터, IIOT, Milk-run AI 알고리즘 등을 활용한 CIPs(Connected Industrial parks) 아키텍쳐가 다양하게 제안되고 있다. 평균적으로 한국의 중소기업은 기술력과 가격 경쟁력 문제로 많은 어려움을 겪고 있다. 미국, 일본, 유럽 등의 해외 선진국들에 비하여 기술력이 확실한 우위를 보이지 못하고 있으며, 중국, 베트남 등의 국가에 비하여는 제조 가격 경쟁력을 보이지 못하고 있다. 이러한 상황에서 한국의 중소 기업들은 지속 가능한 성장 방안을 찾기 위하여 많은 노력을 하고 있다. 재무적으로 한국의 중소기업들이 수익성을 향상시키기 위해서는 매출을 증대시키는 것 보다 비용을 절감하는 것이 효과적이다. 이러한 문제 의식 속에서 한국 CIPs에 위치한 중소 기업들의 비용 절감을 위한 방안으로서 VJP(Vehicle Junction Problem)를 주목하였다. 중소 기업의 최소 물류 비용 달성을 위한 방법으로 CIPs 결제 한국형 시스템을 연구하였다. 새로운 한국형 CIPs결제 시스템의 세부 항목을 크게 4가지 "데이터(Data)", "업무(Business)", "자금(Finance)", "기술(Technique)"로 구분하여 정리하였다.

• 동의생리병리학회지
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• 제18권2호
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• pp.500-506
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• 2004

The objective of the present study was to investigate the effects of aqueous extract from the healthful decoction utilizing Phellinus linteus (HDPL) on the growth of human lung carcinoma A549 cells. HDPL treatment declined the cell viability of A549 cells in a concentration-dependent manner and the anti-proliferative effects by HDPL treatment were associated with morphological changes such as membrane shrinking and cell rounding up. HDPL treatment did not affect the distribution of the cell cycle. Western blot analysis and RT-PCT data revealed that the levels of tumor suppressor p53 and cyclin-dependent kinase inhibitor p21WAF1/CIP1 in HDPL-treated A549 cells were remained unchanged. However, HDPL treatment inhibited the expression of cyclooxygenase-2 (COX-2) mRNA and protein in a concentration-dependent fashion. Additionally, the expression of human telomerase reverse transcriptase (hTERT), a main determinant of the telomerase enzymatic activity, was progressively down-regulated by HDPL treatment. Taken together, these findings suggest that HDPL-induced inhibition of human lung cancer cell proliferation is associated with the inhibition of several major growth regulatory gene products, such as COX-2 and hTERT, and HDPL may have therapeutic potential in human lung cancer.

• Journal of Microbiology and Biotechnology
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• 제16권12호
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• pp.1862-1867
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• 2006

A moderately halophilic, Gram-positive, spore-forming bacterium was isolated from the roots of Blutaparon portulacoides, a plant found in sandy soil parallel to the beach line in Restinga de Jurubatiba, Rio de Janeiro, Brazil. The strain, designated $M9^T$, was motile and strictly aerobic with rod-shaped cells. It grew in the absence of NaCl and up to 20% NaCl, and was able to hydrolyze casein and starch. Strain $M9^T$ had a cell-wall peptidoglycan based on L-Orn-D-Asp, the predominant menaquinone present was menaquinone-7 (MK-7), diaminopimelic acid was not found, and anteiso-$C_{15:0}$ and iso-$C_{15:0}$ were the major fatty acids. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain $M9^T$ belonged to the genus Halobacillus and exhibited 16S rRNA gene similarity levels of 97.8-99.4% with the type strains of the other nine Halobacillus species. The DNA-DNA relatedness of strain $M9^T$ with H. trueperi, the closest relative as regards 16S rRNA gene similarity, and H. locisalis was 21% and 18%, respectively. Therefore, on the basis of phenotypic, genotypic, and phylogenetic data, strain $M9^T$ (=ATCC BAA-$1217^T$, =CIP $108771^T$, =KCTC $3980^T$) should be placed in the genus Halobacillus as a member of a novel species, for which the name Halobacillus blutaparonensis sp. nov. is proposed.

• Biomolecules & Therapeutics
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• 제26권3호
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• pp.328-334
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• 2018

Because of the unsatisfactory treatment options for breast cancer (BC), there is a need to develop novel therapeutic approaches for this malignancy. One such strategy is chemotherapy using non-toxic dietary substances and botanical products. Studies have shown that Panduratin A (PA) possesses many health benefits, including anti-inflammatory, anti-bacterial, anti-oxidant and anticancer activities. In the present study, we provide evidence that PA treatment of MCF-7 BC cells resulted in a time- and dose-dependent inhibition of cell growth with an $IC_{50}$ of $15{\mu}M$ and no to little effect on normal human MCF-10A breast cells. To define the mechanism of these anti-proliferative effects of PA, we determined its effect critical molecular events known to regulate the cell cycle and apoptotic machinery. Immunofluorescence and flow cytometric analysis of Annexin V-FITC staining provided evidence for the induction of apoptosis. PA treatment of BC cells resulted in increased activity/expression of mitochondrial cytochrome C, caspases 7, 8 and 9 with a significant increase in the Bax:Bcl-2 ratio, suggesting the involvement of a mitochondrial-dependent apoptotic pathway. Furthermore, cell cycle analysis using flow cytometry showed that PA treatment of cells resulted in G0/G1 arrest in a dose-dependent manner. Immunoblot analysis data revealed that, in MCF-7 cell lines, PA treatment resulted in the dose-dependent (i) induction of $p21^{WAF1/Cip1}$ and p27Kip1, (ii) downregulation of Cyclin dependent kinase (CDK) 4 and (iii) decrease in cyclin D1. These findings suggest that PA may be an effective therapeutic agent against BC.

• 대한한방내과학회지
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• 제36권1호
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• pp.13-21
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• 2015

Objectives : In Korea, Rhus verniciflua Stokes (RVS) has been used in traditional medicine for various diseases such as back pain, syndromes of the blood system in women, gastrointestinal disease, and cancer. However, the molecular mechanisms of its anti-cancer activity have not been clearly elucidated yet. Methods : This study investigated the possible mechanisms by which RVS extract (RVE) exerts its anti-proliferative action in cultured human breast carcinoma MCF-7 cells. Results : Treatment with RVE in MCF-7 cells resulted in inhibition of cell viability through G1 arrest of the cell cycle and induction of apoptosis in a time- and concentration-dependent manner, as determined by MTT assay and flow cytometry analysis. The induction of G1 arrest by RVE treatment was associated with the inhibition of cyclin D1, cyclin-dependent kinase (Cdk) 2, retinoblastoma protein (pRB), and mouse double minute 2 (MDM2) expression. Moreover, RVE treatment concentration dependently increased the levels of tumor suppressor p53, which was associated with the marked induction of Cdk inhibitors such as p21 (Waf1/Cip1) and p27 (Kip1). However, the inhibition of p53 function by the wild-type p53-specific inhibitor, pifithrin-α, abolished the above-mentioned effects of RVE, showing that p53 was responsible for the cytotoxicity of RVE Conclusions : These data indicate that a molecular pathway involving p53-dependent G1 cell cycle arrest plays a pivotal role in the cellular response to RVE, and demonstrate the potential applications of RVE as an anti-cancer drug for breast cancer treatment.

• 대한의생명과학회지
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• 제20권2호
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• pp.85-95
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• 2014

Activation of the epidermal growth factor receptor (EGFR) and downstream signaling pathways have been implicated in causing resistance to EGFR-targeted therapy in solid tumors, including the head and neck tumors. To investigate the mechanism of antiproliferation to EGFR inhibition in oral cancer, we compared EGFR tyrosine kinase inhibitor (Gefitinib, Iressa, ZD1839) with respect to its inhibitory effects on three kinases situated downstream of EGFR: MAPK, Akt, and glycogen synthase kinase-$3{\beta}$ (GSK-$3{\beta}$). We have demonstrated that ZD1839 induces growth arrest and apotosis in oral cancer cell lines by independent of EGFR-mediated signaling. An exposure of oral cancer cells to ZD1839 resulted in a dose dependent up-regulation of the cyclin-dependent kinase inhibitor p21 and p27, down regulation of cyclin D1, inactivation of GSK-$3{\beta}$ and of active MAPK. In resistant cells, GSK-$3{\beta}$ is constitutively active and its activity is negatively regulated primarily through Ser 9 phosphorylation and further enhanced by Tyr216 phosphorylation. These results showed that the resistance to the antiproliferative effects of ZD1839, in vitro was associated with uncoupling between EGFR and MAPK inhibition, and that GSK-$3{\beta}$ activation and degradation of its target cyclin D1 were indicators of high cell sensitivity to ZD1839. In conclusion, our data show that the uncoupling of EGFR with mitogenic pathways can cause resistance to EGFR inhibition in oral cancer.