• Title/Summary/Keyword: CGT

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Two-Dimensional Analytical Model for Deriving the Threshold Voltage of a Short Channel Fully Depleted Cylindrical/Surrounding Gate MOSFET

  • Suh, Chung-Ha
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.11 no.2
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    • pp.111-120
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    • 2011
  • A two-dimensional analytical model for deriving the threshold voltage of a short channel fully depleted (FD) cylindrical/surrounding gate MOSFET (CGT/SGT) is suggested. By taking into account the lateral variation of the surface potential, introducing the natural length expression, and using the Bessel functions of the first and the second kinds of order zero, we can derive potentials in the gate oxide layer and the silicon core fully two-dimensionally. Making use of these potentials, the minimum surface potential can be obtained to derive the threshold voltage as a closed-form expression in terms of various device parameters and applied voltages. Obtained results can be used to explain the drain-induced threshold voltage roll-off of a CGT/SGT in a unified manner.

Expression and Optimum Production of Cyclodextrin Glucanotransferase Gene of Paenibacillus sp. JB-13 in E. coli (Paenibacillus sp. JB-13 Cyclodextrin Glucanotransferase 유전자의 E. coli 에서의 발현 및 최적 생산)

  • Kim, Hae-Yun;Lee, Sang-Hyeon;Kim, Hae-Nam;Min, Bok-Kee;Baik, Hyung-Suk;Jun, Hong-Ki
    • Korean Journal of Microbiology
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    • v.44 no.1
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    • pp.74-79
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    • 2008
  • The purpose of this study is to clone cgt gene from Paenibacillus sp. JB-13 and to overexpress the protein in E. coli. For this purpose, the cgt gene was amplified from Paenibacillus sp. JB-13 genomic DNA by PCR using degenerate oligonucleotide primers. The sequence analysis results showed that the cgt gene from Paenibacillus sp. JB-13 has 98% homology with the cgt gene of Bacillus sp. To overexpress the protein, the cgt gene was cloned into pEXP7 expression vector and transformed into E. coli. The production of CGTase by recombinant E. coli was optimized under following conditions: 0.5% glucose, 3.0% polypeptone, 0.3% $K_2HPO_4$, 0.5% NaCl, and 7.0 of initial pH, 2.0% of inoculum, $37^{\circ}C$ of culture temperature for 14 hr. And the optimal agitation was found at 0.1 vvm. The synthesis of 2-O-${\alpha}$-D-Glucopyranosyl L-Ascorbic acid (AA-2G) using the CGTase expressed in E. coli was identified as AA-2G by HPLC and HPLC confirmed that treating AA-2G made by cloned CGTase with ${\alpha}$-glucosidase substantially produced AA and glucose.

Constitutive Expression of Bacillus stearothermophilus CGTase in Bacillus subtilis. (Bacillus subtilis에서 Bacillus stearothermophilus CGTase의 구성적 발현)

  • 허선연;김중균;권현주;김병우;김동은;남수완
    • Journal of Life Science
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    • v.14 no.3
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    • pp.391-395
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    • 2004
  • To overproduce the cyclodextrin glucanotransferase(CGTase) of Bacillus stearothermophilus NO2 in B. subtilis, the pJH-CGTl plasmid (8.14 kb) was constructed, in which the ORF of CGTase gene could be transcribed by strong constitutive promoter, P$\_$JH/. To overproduce CGTase from a recombinant B. subtilis, the effect of media on the cell growth and expression level of CGTase were investigated with various media (LB, 2${\times}$LB, 5% molasses+2% CSL, CS, LBG) in the flask culture. Among them, [5% molasses+2% CSL] medium revealed the maximum expression level of CGTase with 1.8 unit/$m\ell$ at 9 hr culture. In the batch culture on [10% molasses+5% corn steep liquor] medium the expression level of CGTase, the secretion efficiency, and plasmid stability were about 4.2 unit/$m\ell$, 90% and 90%, respectively, at 30 hr culture. The cell growth and expression level in the fermenter culture with the industrial molasses medium were increased by 2-folds over the flask culture.

Assessment of Antarctic Ice Tongue Areas Using Sentinel-1 SAR on Google Earth Engine (Google Earth Engine의 Sentienl-1 SAR를 활용한 남극 빙설 면적 변화 모니터링)

  • Na-Mi Lee;Seung Hee Kim;Hyun-Cheol Kim
    • Korean Journal of Remote Sensing
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    • v.40 no.3
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    • pp.285-293
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    • 2024
  • This study explores the use of Sentinel-1 Synthetic Aperture Radar (SAR), processed through Google Earth Engine (GEE), to monitor changes in the areas of Antarctic ice shelves. Focusing on the Campbell Glacier Tongue (CGT) and Drygalski Ice Tongue (DIT),the research utilizes GEE's cloud computing capabilities to handle and analyze large datasets. The study employs Otsu's method for image binarization to distinguish ice shelves from the ocean and mitigates detection errors by averaging monthly images and extracting main regions. Results indicate that the CGT area decreased by approximately 26% from January 2016 to January 2024, primarily due to calving events,while DIT showed a slight increase overall,with notable reduction in recent years. Validation against Sentinel-2 optical images demonstrates high accuracy,underscoring the effectiveness of SAR and GEE for continuous, long-term monitoring of Antarctic ice shelves.

Analytical Modeling and Simulation for Dual Metal Gate Stack Architecture (DMGSA) Cylindrical/Surrounded Gate MOSFET

  • Ghosh, Pujarini;Haldar, Subhasis;Gupta, R.S.;Gupta, Mridula
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.12 no.4
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    • pp.458-466
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    • 2012
  • A Dual metal gate stack cylindrical/ surrounded gate MOSFET (DMGSA CGT/SGT MOSFET) has been proposed and an analytical model has been developed to examine the impact of this structure in suppressing short channel effects and in enhancing the device performance. It is demonstrated that incorporation of gate stack along with dual metal gate architecture results in improvement in short channel immunity. It is also examined that for DMGSA CGT/SGT the minimum surface potential in the channel reduces, resulting increase in electron velocity and thereby improving the carrier transport efficiency. Furthermore, the device has been analyzed at different bias point for both single material gate stack architecture (SMGSA) and dual material gate stack architecture (DMGSA) and found that DMGSA has superior characteristics as compared to SMGSA devices. The analytical results obtained from the proposed model agree well with the simulated results obtained from 3D ATLAS Device simulator.

Identification of AFLP Marker Linked to a SCN Resistant Gene in Soybean

  • Ko, Mi-Suk;Kim, Myung-Sik;Han, Soung-Jin;Chung, Jong-Il;Kang, Jin-Ho
    • Plant Resources
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    • v.5 no.3
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    • pp.169-175
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    • 2002
  • The soybean cyst nematode (Heterodera glycines Inchinoe; SCN) is a devastating pest of soybean and is responsible for significant losses in yield. The use of resistant cultivars is the effective method to reduce or eliminate SCN damage. The objective of this research is to identify AFLP markers linked to the SCN resistant genes. Bulked genomic DNA was made from resistant and susceptible genotypes to SCN and a total of 19 primer combinations were used. About 31 fragments were detected per primer combination. The banding patterns were readily distinguished in resistant and susceptible bulked genotypes. Polymorphic fragments were detected between resistant and susceptible bulked genotypes in the primer combination of CGT/GGC, CAG/GTG and CTC/GAG. In primer combinations of CGT/GGC and CAG/GTG, bulked resistant genotype produced a polymorphic bands. However, in primer of CTC/GAG, bulked susceptible genotype produced a polymorphic fragments. Three AFLP markers identified as a polymorphic fragments between bulked genomic DNA were mapped in 85 F2 population. Among them, only two markers, CGT/GGC and CTC/GAG, was linked and was mapped. Broad application of AFLP marker would be possible for improving resistant cultivars to SCN.

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Study of The Correlation of Lung-Large intestine-Skin by Ulcerative Colitis-Induced 3 Weeks Old Mice (3주된 생쥐에서 궤양성 대장염 유발을 통한 폐-대장-피부의 상관관계 연구)

  • Ahn, Sang Hyun;Kim, Kibong
    • The Journal of Pediatrics of Korean Medicine
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    • v.33 no.3
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    • pp.103-111
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    • 2019
  • Objectives The purpose of this study is to understand the correlations between lung, large intestine, and skin of 3-week-old mice in which ulcerative colitis was induced, up on administration of Coptidis rhizome and Glycyrrhiza uralensis mixed extract. Methods Mice were divided into 4 groups as follows; no treatment group (Ctrl group), ulcerative colitis-induced mice group (UE group), ulcerative colitis-induced mice group after administering Pentasa (PT group), ulcerative colitis-induced mice group after administering Coptidis rhizoma and Glycyrrhiza uralensis mixed extract (CGT group). Mice were induced ulcerative colitis by Dextran sulfate sodium (DSS). After 5 days of administration, We obvserved anti-inflammatory effect, alveolar formation, and skin barrier control in the colon mucosa. Results The CGT group was observed arrangement of normal intestinal cells, Infiltration of less inflammatory cells. The CGT significantly decreased positive rseponse of $TNF-{\alpha}$, p-IkB, Caspase 3 in large intestine, and significantly increased positive rseponse of EGF, IGF, catalase, Filaggrin, involucrin, loricrin. Conclusions The results of this study show the correlation of Lung-Large intestine-Skin by administering Coptidis rhizoma and Glycyrrhiza uralensis mixed extract to ulcerative colitis-induced mice.

Application of Seed Vigor Test for Predicting Field Emergence in Azuki Bean (Vigna angularis Wight) (팥 포장출현력 예측을 위한 종자세 검사)

  • Jeong, Gwan-Seok;Na, Young-Wang;Shim, Sang-In;Kim, Seok-Hyeon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.3
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    • pp.341-349
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    • 2014
  • Field emergence of Azuki bean is poor due to hard seed coat as compared to other legumes. In this study, an attempt was made to develop prediction method with regression analysis based on various seed vigor tests in laboratory for field emergence of azuki bean. Azuki bean seeds artificially aged to provide various levels of seed quality were evaluated by the standard germination test (SGT), cold germination test (CT), cool germination test (CGT), complex stressing vigor test (CSVT), tetrazolium(TZ) vigor test and electroconductivity test. The SGT was suitable for predicting the field emergence in the unaged high vigor seeds. The abnormal seedling percentage and shoot length in the CGT were highly correlated with field emergence of moderate vigor seeds artificially aged for 2 days. Electroconductivity, seed viability in the CSVT, and vigor and predicted germinability in the tetrazolium vigor test were also useful for predicting field emergence. Percent of ungerminated seed in the CSVT was correlated with field emergence in the low vigor seeds artificially aged for 4 days. In a stepwise multiple regression analysis, seed viability in the SGT, normal seedling percentage and dry matter weight in the CGT accounted for 86.9% of the predicted value of field emergence in azuki bean.

Effect of Skin Fat Lipid Barrier Formation on Hataedock with Coptis Japonica & Glycyrrhiza Uralensis (황련감초 하태독법의 피부 지방장벽형성 증진효과)

  • Ahn, Sang Hyun;Kim, Ki Bong
    • The Journal of Pediatrics of Korean Medicine
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    • v.31 no.3
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    • pp.14-23
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    • 2017
  • Objectives This study is conducted to evaluate skin fat barrier formation of Hataedock using the Coptis japonica & Glycyrrhiza uralensis extract. Methods The 3-week-old NC/Nga mice were divided into 3 groups: control group (Ctrl), Hataedock-treated group that uses the Coptis japonica & Glycyrrhiza uralensis (CGT) extract, and Hataedock-treated group that uses Bifidobacterium (BBT). After 2 weeks, changes in immunohistochemicals, and skin-lipid-barrier regulators were observed for the effects of Hataedock. Results In CGT group, loricrin-positive reaction has been increased by 231%, along with involucrin-positive reaction by 90%, filaggrin-positive reaction by 143%, and ASM-positive reaction by 341% in the stratum corneum. Conclusions Hataedock, using the extract of Coptis japonica & Glycyrrhiza uralensis, increased the expression of proteins promoting keratinocyte differentiation. This leads into conclusion that Hataedock may increase the keratinocyte formation and function which promotes skin barrier formation.

ADAPTIVE CONTROL SYSTEM DESIGN BASED ON CGT ATTROACH

  • Ohtsuka, H.;Mizumoto, I.;Iwai, Z.
    • 제어로봇시스템학회:학술대회논문집
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    • 1994.10a
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    • pp.189-194
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    • 1994
  • Adaptive control systems based upon the command generator tracker(CGT) approach have attracted considerable interest because of the simple structure of its adaptive controller. Some attempts to such improve the adaptive control algorithm, for the sake of the application to broader class of plants, are made. Recently, Su and Sobel(1992) proposed that those schemes can be treated by an unified theory using a metasystem representation with some types of supplementary dynamics. However, in their method, it is difficult to find the dynamic compensator, which is proper and output feedback stabilizable, for the uncertain plant. This paper proposes a new design method of such supplementary dynamics and some parameters of adaptive control system for linear time invariant SISO plants. The method gives a concrete and systematic design method using only a few priori knowledge of the plant.

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