• 제목/요약/키워드: CD18

검색결과 797건 처리시간 0.022초

Adhesion of CD40-stimulated Germinal Center B Cells to HK Cells Employs the CD11a/CD18-CD54 Interactions

  • Lee, Joonhee;Choe, Jongseon
    • IMMUNE NETWORK
    • /
    • 제3권3호
    • /
    • pp.176-181
    • /
    • 2003
  • Background: The molecular basis of follicular dendritic cells (FDC)-germinal center (GC) B cell interaction is largely unknown, although this cellular interaction is thought to be important for the whole process of GC B cell differentiation. Methods: Using FDC-like cells, HK, and highly purified GC B cells, we attempted to identify the molecules that play critical roles in the interactions between FDC and B cells. GC B cells were co-cultured with HK cells and soluble CD154 in the presence or absence of various function-blocking monoclonal antibodies to examine their effect on GC B cell binding to HK cells and B cell proliferation. Results: Anti-CD11a and anti-CD54 antibodies inhibited GC B cell binding to HK cells while anti-CD49d and anti-CD106 antibodies did not. GC B cell proliferation was not impaired by the disruption of GC B cell-HK cell adherence. Conclusion: Our results suggest that CD11a/CD18-CD54 interactions play an important roles in the initial binding of GC B cells to FDC and diffusible growth factors from FDC may be responsible the massive proliferation of GC B cells.

CdS 박막의 제조 방법에 따른 물성 및 CdS/CdTe 이종접합의 전기적 특성 분석 (Characterization of CdS Thin Films and CdS/CdTe Heterojunction Prepared by Different Techniques)

  • 이재형
    • 한국전기전자재료학회논문지
    • /
    • 제18권3호
    • /
    • pp.199-205
    • /
    • 2005
  • Polycrystalline cadmium sulfide(CdS) thin films were deposited on glass substrate by chemical bath deposition(CBD) and vacuum evaporation (VE) techniques. VE-CdS films consisted primarily of hexagonal phase, whereas CBD CdS films containing primarily the cubic form. VE-grown films were shown to have better crystallinity than CBD-grown films. The grain size of the CBD films is smaller than the ones of VE films. VE-CdS films exhibited relatively high transmittance in the above-gap region and band gap compared with CBD films. However, CdTe solar cells with these low quality CBD-CdS layers yield higher and more stable characteristics. Current-voltage-temperature measurements showed that the current transport for both cells was controlled by both tunneling and interface recombination but the cells with CBD-CdS displayed less tunneling.

흰쥐의 복직근피부피판에 일으킨 허혈-재관류 손상에서 CD18의 발현 (The Expression of CD 18 on Ischemia- Reperfusion Injury of TRAM Flap of Rats)

  • 윤상엽;이택종;홍준표
    • Archives of Plastic Surgery
    • /
    • 제33권6호
    • /
    • pp.737-741
    • /
    • 2006
  • Purpose: This study was to evaluate the expression pattern of CD 18(leukocyte adhesion glycoprotein) in ischemia-reperfusion injury of TRAM flap of rats. Through this study, we can obtain more information about ischemia-reperfusion injury. We want to develop specific medicine to improve the survival rate of TRAM flap in the future. Methods: A TRAM flap supplied by a single pedicle superior epigastric artery and vein was elevated on 60 Sprauge-Dawley rats. The rats were divide into 6 groups (each group n=10); Group O: sham, no ischemia-reperfusion injury, Group I: 2 hour reperfusion after 4 hour ischemia, Group II: 4 hour reperfusion after 4 hour ischemia, Group III: 8 hour reperfusion after 4 hour ischemia, Group IV: 12 hour reperfusion after 4 hour ischemia, and Group V: 24 hour reperfusion after 4 hour ischemia. This study consisted of gross examination for flap survival and flow cytometry study of CD18 on neutrophils. Results: The gross measurement of the flap showed different survival rate in group I(71%), II(68%), III(37%), IV(34%) and V(34%). All experimental groups showed an increase in the expression of CD18 compared to group O. The expression of CD18 was rapidly increased in ascending order in group I, II and III. But, the expression of CD18 was maintained in group IV and V. Conclusion: The results can be implemented in the study to develop drugs which are capable of reducing ischemia-reperfusion injury in microsurgical breast reconstruction.

Roles of Neutral Sphingomyelinase 1 on CD95-Mediated Apoptosis in Human Jurkat T Lymphocytes

  • Lee, Hyun-Min;Surh, Bo-Young;Chun, Young-Jin
    • Biomolecules & Therapeutics
    • /
    • 제18권3호
    • /
    • pp.262-270
    • /
    • 2010
  • CD95 receptor is a member of tumor necrosis factor receptor family that mediates apoptosis in many cell types when bound by CD95 ligand or cross-linked by agonistic anti-CD95 antibodies. To determine the role of neutral sphingomyelinase (nSMase) on CD95-mediatd apoptosis, human Jurkat T lymphocytes were exposed to recombinant human CD95 ligand. Treatment with CD95 ligand induced cell death in a concentration and time-dependent manner. CD95-induced cell death was suppressed by inhibitors of SMase such as AY9944 or desipramine. Transfection with human nSMase1 siRNA plasmid into CD95 ligand-treated cells significantly prevented CD95-mediated cell death. CD95-mediated elevation of intracellular ceramide level detected by FACS analysis with anti-ceramide antibody was also decreased by nSMase1 siRNA. Knock-down of nSMase1 expression also blocked cytochrome c release into cytosol and caspase-3 cleavage in CD95-treated cells. Taken together, these results suggest that nSMase1 may play an important role in CD95-mediated apoptotic cell death in Jurkat T cells.

도축 사슴의 체조직내 카드뮴 함량에 관한 조사 연구 (Cadmium contents in tissues of slaughtered deer in Korea)

  • 장종식;권오덕
    • 한국동물위생학회지
    • /
    • 제24권1호
    • /
    • pp.95-99
    • /
    • 2001
  • In order to clarify the cadmium(Cd) contents of normal deer, tissue samples including kidney, liver, muscle, spleen, heart, lung, rumen, abomasum, intestine, skin and bone were collected from 18 deer which were slaughtered in Korea. All samples were burned to ashes and analyzed for Cd contents by atomic absorption spectrophotometric method. The mean Cd contents in \mu\textrm{g}$/g wet matter for 18 deer were liver 0.013, kidney 0.286, muscle 0.010, spleen 0.001, heart 0.007, lung 0.005, men 0.019, abomasum 0.016, intestine 0.018, skin 0.016 and bone 0.312, respectively. Two samples in kidney, one sample in muscle out of 18 deer showed higher Cd contents than normal limit value of 0.50\mu\textrm{g}$/g for liver and kidney, 0.10\mu\textrm{g}$/g for muscle. In addition, three deer also showed higher Cd contents in one body tissue than normal limit value which reported foreign countries.

  • PDF

닭의장풀에 Cd2+ 처리시 Cd2+ 흡수와 생장에 미치는 indole acetic acid의 영향 (The effect of Indole acetic acid on the accumulation of Cd2+ and growth of Cd2+ - treated Commelina communis L.)

  • 이준상
    • 한국환경과학회지
    • /
    • 제13권6호
    • /
    • pp.513-518
    • /
    • 2004
  • 3-weeks old Commelina was transferred to and grown in Hoagland solution (Control, $100\muM$ $Cd^{2+}$, $100\muM$ $Cd^{2+}$+ $100\muM$ IAA, $100\muM$ $Cd^{2+}$+ $100\muM$ IAA + 2 mM sucrose) for 3 weeks and then the effects of indole acetic acid (IAA) on the accumulation of $Cd^{2+}$ and growth of $Cd^{2+}$-treated Commelina were investigated. In the treatment of $Cd^{2+}$, $Cd^{2+}$ was uptaked to 1.74, and 51.36 ${\mu}g/g$ frwt. at the first week, but for three weeks, 0.51 and 34,53 ${\mu}g/g$ frwt, in leaf and stem respectively. When IAA was treated along with $Cd^{2+}$, $Cd^{2+}$ was uptaked to 0.18 and 8.63 ${\mu}g/g$ fiwt, at the first week, and for the incubation of 3 weeks, 0,51 and 45.0 ${\mu}g/g$ fiwt. in leaf and stem. In case of $Cd^{2+}$+IAA+sucrose, $Cd^{2+}$ was uptaked to 1.45 and 18.33 ${\mu}g/g$ frwt. at the first week, but for 3 weeks, 0,51 and 25.45 ${\mu}g/g$ fiwt. in leaf and stem. Likewise $Cd^{2+}$ uptake, the growth was also affected by $Cd^{2+}$ and IAA. During the incubation of 3 weeks, $Cd^{2+}$ reduced the stem growth about 8% in all weeks, but the treatment of IAA recovered the inhibition of stem growth caused by $Cd^{2+}$ to the degree of the control Therefore, it could be concluded that IAA altered the pattern of $Cd^{2+}$ uptake and the growth which were supposed to change $Cd^{2+}$ toxicity.

CD-ROM과 멀티미디어

  • 한국데이터베이스진흥센터
    • 디지털콘텐츠
    • /
    • 12호통권7호
    • /
    • pp.18-18
    • /
    • 1993
  • 93년 11월 멀티미디어 전문 리포트인 멀티미디어 모니터에 충격적인 기사가 실렸다. 영어를 그대로 사용하면 "In the CD-ROM market, all that glitters is not consumer multimedia"이다. 내용을 일부 요약하면 다음과 같다.

  • PDF

명소시 상태에서 휘도 감소에 따른 대비감도 변화 (Changes of Contrast Sensitivity with Decreasing Luminance in Photopic Conditions)

  • 이은진;윤문주;김수현;양계탁;정주현;김현정;김현일;최은정
    • 한국안광학회지
    • /
    • 제17권4호
    • /
    • pp.411-417
    • /
    • 2012
  • 목적: 명소시 상태에서 휘도의 감소에 따른 대비감도 변화에 대하여 조사하였다. 방법: FACT 시표를 이용하여 명소시 5단계의 휘도 하에서 63명의 대학생에 대한 대비감도를 조사하였다. 결과: 측정된 대비감도는 모두 정상범위 내에 있었다. $40~10cd/m^2$에서 대비감도의 정점이 6 cpd에서 3 cpd로 이동하는 것으로 관측되었고, 6 cpd와 3 cpd에 대한 가상적인 두 대비감도 직선들의 교점으로부터 정점은 약 $25cd/m^2$일때 6 cpd에서 3 cpd로 이동하는 것으로 평가되었다. 휘도가 $120cd/m^2$에서 $10cd/m^2$으로 감소하는 동안, 6 cpd에서 대비감도 손실이 가장 컸지만, 1.5 cpd에서의 대비감도는 큰 변화가 없었다. 근사적으로 평가된 $25cd/m^2$ 이상의 휘도에서 대비감도는 18<12<1.5<3<6 cpd의 순으로 높았지만, $25cd/m^2$이하의 휘도에서는 18<12<1.5<6<3 cpd의 순으로 높았다. 휘도의 감소에 따른 대비감도의 손실을 대비감도 감소량 및 감소율로도 평가하였다. 대비감도 감소량은 6 cpd에서 가장 컸지만, 감소율은 18 cpd에서 가장 컸다. 결론: 본 연구에서 활용된 평가법이 대비감도 변화를 평가하는 또 다른 방법으로 활용될 수 있음을 제안한다.