Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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The Expression of CD 18 on Ischemia- Reperfusion Injury of TRAM Flap of Rats

흰쥐의 복직근피부피판에 일으킨 허혈-재관류 손상에서 CD18의 발현

  • Yoon, Sang Yup (Baram Clinic BBC) ;
  • Lee, Taik Jong (Asan Medical Center, Department of Plastic Surgery, University of Ulsan, College of Medicine) ;
  • Hong, Joon Pio (Asan Medical Center, Department of Plastic Surgery, University of Ulsan, College of Medicine)
  • 윤상엽 (바람성형외과) ;
  • 이택종 (서울아산병원 울산대학교 의과대학 성형외과학교실) ;
  • 홍준표 (서울아산병원 울산대학교 의과대학 성형외과학교실)
  • Received : 2006.04.07
  • Published : 2006.11.10
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Abstract

Purpose: This study was to evaluate the expression pattern of CD 18(leukocyte adhesion glycoprotein) in ischemia-reperfusion injury of TRAM flap of rats. Through this study, we can obtain more information about ischemia-reperfusion injury. We want to develop specific medicine to improve the survival rate of TRAM flap in the future. Methods: A TRAM flap supplied by a single pedicle superior epigastric artery and vein was elevated on 60 Sprauge-Dawley rats. The rats were divide into 6 groups (each group n=10); Group O: sham, no ischemia-reperfusion injury, Group I: 2 hour reperfusion after 4 hour ischemia, Group II: 4 hour reperfusion after 4 hour ischemia, Group III: 8 hour reperfusion after 4 hour ischemia, Group IV: 12 hour reperfusion after 4 hour ischemia, and Group V: 24 hour reperfusion after 4 hour ischemia. This study consisted of gross examination for flap survival and flow cytometry study of CD18 on neutrophils. Results: The gross measurement of the flap showed different survival rate in group I(71%), II(68%), III(37%), IV(34%) and V(34%). All experimental groups showed an increase in the expression of CD18 compared to group O. The expression of CD18 was rapidly increased in ascending order in group I, II and III. But, the expression of CD18 was maintained in group IV and V. Conclusion: The results can be implemented in the study to develop drugs which are capable of reducing ischemia-reperfusion injury in microsurgical breast reconstruction.

Keywords

References

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