• BMB Reports
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• v.41 no.1
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• pp.62-67
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• 2008

The present study was aimed to elucidate the mechanism of stabilization of tubulin by deuterium oxide ($D_2O$). Rate of decrease of tryptophan fluorescence during aging of tubulin at 4$^{\circ}C$ and 37$^{\circ}C$ was significantly lower in $D_2O$ than in $H_2O$. Circular dichroism spectra of tubulin after incubation at 4$^{\circ}C$, suggested that complete stabilization of the secondary structure in D2O during the first 24 hours of incubation. The number of available cysteine measured by DTNB reaction was decreased to a lesser extent in $D_2O$ than in $H_2O$. . During the increase in temperature of tubulin, the rate of decrease of fluorescence at 335 nm and change of CD value at 222 nm was lesser in $D_2O$. Differential Scanning calorimetric experiments showed that the $T_m$ values for tubulin unfolding in $D_2O$ were 58.6$^{\circ}C$ and 62.17$^{\circ}C$, and in $H_2O$. those values were 55.4$^{\circ}C$ and 59.35$^{\circ}C$.

• BMB Reports
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• v.40 no.3
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• pp.412-418
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• 2007

Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is an unique antioxidant enzyme that directly reduces lipid hydroperoxides in biomembranes. In the present work, the entire encoding region for Oryza sativa PHGPx was expressed in Escherichia coli M15, and the purified fusion protein showed a single band with 21.0 kD and pI = 8.5 on SDS- and IFE-PAGE, respectively. Judging from CD and fluorescence spectroscopy, this protein is considered to have a well-ordered structure with 12.2% $\alpha$-helix, 30.7%$\beta$-sheet, 18.5% $\delta$-turn, and 38.5% random coil. The optimum pH and temperature of the enzyme activity were pH 9.3 and 27$^{\circ}C$. The enzyme exhibited the highest affinity and catalytical efficiency to phospholipid hydroperoxide employing GSH or Trx as electron donor. Moreover, the protein displayed higher GSH-dependent activity towards t-Butyl-OOH and $H_2O_2$. These results show that OsPHGPx is an enzyme with broad specificity for hydroperoxide substrates and yielded significant insight into the physicochemical properties and the dynamics of OsPHGPx.

• Journal of Korean Society on Water Environment
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• v.33 no.4
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• pp.424-433
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• 2017

The purpose of this study is to understand the changes on species composition of the epilithic diatoms and the appearance of morphological abnormalities in the upper region of the Nakdong River where heavy metal inflow is observed. The samples were collected once a week for a month of September 2016 from selecting 7 sampling stations. The heavy metals of cadmium (Cd) and arsenic (As) were not detected during the survey period, but zinc (Zn) was detected in st.4 - st.6 with the range of $0.015{\sim}0.188mg\;L^{-1}$. Metal sensitive species such as Achnanthes convergens, Cocconeis placentula, Cocconeis placentula var. euglypta, Cocconeis placentula var. lineata showed high dominance in st.1 - st.3, st.7. However, metal tolerant species such as Nitzschia palea, Achnanthes minutissima showed high dominance in st.4 - st.6. It is concluded that heavy metal inflow directly affects the changes in species composition of epilithic diatoms. As a result of CCA, the characteristics of the sampling sites were divided into 3 groups. Group 1 was represented the non-detected Zn sites with C. placentula, C. placentula var. euglypta, C. placentula var. lineata. Group 2 was showed the detected Zn sites with Navicula minima and Nitzschia palea. Group 3 was included st.3 - st.7 on 4th week that was stabilized the community structure. Total 8 taxa of abnormal frustules observed. This occurrence of abnormal frustules reflected the temporal and quantitative indicators of heavy metal pollution, in particular, it was confirmed that genus Fragilaria, which has a high abnormality according to heavy metal pollution, can be used as an indicator species.

• The Bulletin of The Korean Astronomical Society
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• v.35 no.1
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• pp.39.2-39.2
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• 2010

In this paper, I present the domestic development of near infrared camera systems for the ground telescope and the space satellite. These systems are the first infrared instruments made for astronomical observation in Korea. KASINICS (KASI Near Infrared Camera System) was developed to be installed on the 1.8m telescope of the Bohyunsan Optical Astronomy Observatory (BOAO) in Korea. KASINICS is equipped with a $512{\times}512$ InSb array enable L band observations as well as J, H, and Ks bands. The field-of-view of the array is $3.3'{\times}3.3'$ with a resolution of 0.39"/pixel. It employs an Offner relay optical system providing a cold stop to eliminate thermal background emission from the telescope structures. From the test observation, limiting magnitudes are J=17.6, H=17.5, Ks=16.1 and L(narrow)=10.0 mag at a signal-to-noise ratio of 10 in an integration time of 100 s. MIRIS (Multi-purpose InfraRed Imaging System) is the main payload of the STSAT-3 in Korea. MIRIS Space Observation Camera (SOC) covers the observation wavelength from $0.9{\mu}m$ to $2.0{\mu}m$ with a wide field of view $3.67^{\circ}{\times}3.67^{\circ}$. The PICNIC HgCdTe detector in a cold box is cooled down below 100K by a micro Stirling cooler of which cooling capacity is 220mW at 77K. MIRIS SOC adopts passive cooling technique to chill the telescope below 200K by pointing to the deep space (3K). The cooling mechanism employs a radiator, a Winston cone baffle, a thermal shield, MLI of 30 layers, and GFRP pipe support in the system. Opto-mechanical analysis was made in order to estimate and compensate possible stresses from the thermal contraction of mounting parts at cryogenic temperatures. Finite Element Analysis (FEA) of mechanical structure was also conducted to ensure safety and stability in launching environments and in orbit. MIRIS SOC will mainly perform the Galactic plane survey with narrow band filters (Pa $\alpha$ and Pa $\alpha$ continuum) and CIB (Cosmic Infrared Background) observation with wide band filters (I and H) driven by a cryogenic stepping motor.

• The Bulletin of The Korean Astronomical Society
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• v.35 no.1
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• pp.40.2-40.2
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• 2010

MIRIS 우주관측카메라는 과학기술위성 3호의 주탑재체로서 $0.8{\sim}2.0{\mu}m$의 근적외선영역에서 우주배경복사와 우리은하 평면의 Pa-$\alpha$ survey 관측을 목적으로 한다. 이러한 임무를 수행하기 위해 MIRIS 우주관측카메라에는 MCT(HgCdTe) IR 검출기가 사용되고 6개의 필터를 장착할 수 있는 필터휠이 설계되었으며, 열잡음을 줄이고 원하는 SNR을 얻기 위해 모두 100K 이하로 냉각이 요구된다. 효과적인 냉각 및 저온유지를 위해서 외부의 열을 1차적으로 차단하는 Cryostat 외부용기와 100K 이하로 냉각되는 내부 Cold Box의 이중구조를 가지는 Dewar가 설계 되었다. 내부 Cold Box의 냉각은 소형 stirling cooler로 이루어지고 외부의 열 유입량이 Cooler의 냉각용량을 넘지 않도록 설계하였다. Cryostat 외부용기는 radiation cooling으로 냉각되어 200K 이하의 온도를 유지하며 내부 Cold Box로의 열유입을 최소화하기 위해 GFRP(Glass Fiber Reinforced Plastic) 단열 지지대와 MLI(Multi Layer Insulation)가 사용된다. 또한 100K으로 냉각시 필터고정부와 Cold Box 구조에서 일어날 수 있는 구조적인 피로도를 줄이고 열변형에 의한 문제를 방지하기 위한 고려가 설계에 포함되었다. FM(Flight Model)은 고진공 환경의 우주공간에서 문제가 발생하지 않도록 설계되었다. 또한 EQM 진동시험결과를 토대로 발사환경에서 발생하는 강한 진동을 견딜 수 있도록 FEM(Finite Elements Method) 구조해석을 통하여 필터고정부에 flexible structure 설계와 완충제를 추가하고 필터휠 구동부와 harness 고정부 및 cooler 지지부를 비롯한 전체 구조물에서 충분히 진동을 극복할 수 있도록 설계하였다.

• Journal of the Korean Geotechnical Society
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• v.21 no.2
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• pp.137-144
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• 2005

A direct shear test is classified roughly by one side simple shear test of confining horizontal displacement type and torsional shear test of non-confining one. Direct shear test that has been widely used so far has some problems with test apparatus, testing and the analysis, and in particular that its strength value is everestimated in sandy soils. Also, progressive failure of shearing process happens from shear apparatus restriction and because the shear strain and shear stress are erratic in specimen, we can not define the shear strain value. In the meantime, a simple shear test having advantage of direct shear test is an ideal test method that can get stress-strain relation on shear because it can deliver constant shearing deformation to specimen. However, simple shear test cannot be used practically, because its structure makes tester manufacturing difficult. This paper described a on outline of test apparatus, improvement of test method, and constant pressure test results based on the obtained from improved direct shear apparatus and the standardization of JGS soil testing method.

• BMB Reports
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• v.41 no.5
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• pp.369-375
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• 2008

Polygonatum cyrtonema Lectin (PCL), which is classified as a monocot mannose-binding lectin, has received great regards for its uniquely biological activities and potentially medical applications in cancer cells. This paper was initially aimed to study apoptosis of PCL on Hela cells. Thus, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method was carried out. Through observation of cell morphologic changes and Lactate dehydrogenase (LDH) activity-based cytotoxicity assays, PCL induced HeLa cell apoptosis in a dose-dependent manner. To further gain structural basis, multiple alignments, homology modeling and docking experiments were performed to analyze the correlation between its biological activities and mannose-binding sites. Eventually, considering docking data, chemical modification properties on the three mannose-binding sites were analyzed by a series of biological experiments (e.g., hemagglutinating and mitogenic activity assays, fluorescence and Circular Dichrosim (CD) spectroscopy) to profoundly identify the role of some key amino acids in the structure-function relationship of PCL.

• Journal of the Korean institute of surface engineering
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• v.45 no.1
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• pp.20-24
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• 2012

Multilayer passivation film on OLED with organic/inorganic hybrid structure as to diminish the thermal stress and expansion was researched to protect device from the direct damage of $O_2$ and $H_2O$ and improve life time characteristics. Red OLED doped with 1 vol.% Rubrene in $Alq_3$ was used as a basic device. The films consist of ITO(150 nm)/ELM200_HIL(50 nm)/ELM002_HTL(30 nm)/$Alq_3$: 1 vol.% Rubrene(30 nm)/$Alq_3$(30 nm) and LiF(0.7 nm)/Al(100 nm) which were formed in that order. Using LiF/$SiN_x$ as a buffer layer was determined because it significantly improved life time characteristics without suffering damage in the process of forming passivation film. Multilayer passivation film on buffer layer didn't produce much change in current efficiency, while the half life time at 1,000 $cd/m^2$ of OLED/LiF/$SiN_x$/E1/$SiN_x$ was 710 hours which showed about 1.5 times longer than OLED/LiF/$SiN_x$/E1 with 498 hours. futhermore, OLED/LiF/$SiN_x$/E1/$SiN_x$/E1/$SiN_x$ with 1301 hours showed about twice than OLED/LiF/$SiN_x$/E1/$SiN_x$ which demonstrated that superior characteristics of life time was obtained in multilayer passivation film. Through the above result, it was suggested using LiF/$SiN_x$ as a buffer layer could reduce the damage from the difference of thermal expansion coefficient in OLED with protective films, and epoxy layer in multilayer passivation film could function like a buffer between $SiN_x$ inorganic layers with relatively large thermal stress.

• Fisheries and Aquatic Sciences
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• v.20 no.7
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• pp.9.1-9.13
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• 2017

Background: Metal-responsive transcription factor-1 (MTF-1) is a key transcriptional regulator playing crucial roles in metal homeostasis and cellular adaptation to diverse oxidative stresses. In order to understand cellular pathways associated with metal regulation and stress responses in Pacific abalone (Haliotis discus hannai), this study was aimed to isolate the genetic determinant of abalone MTF-1 and to examine its expression characteristics under basal and experimentally stimulated conditions. Results: The abalone MTF-1 shared conserved features in zinc-finger DNA binding domain with its orthologs; however, it represented a non-conservative shape in presumed transactivation domain region with the lack of typical motifs for nuclear export signal (NES) and Cys-cluster. Abalone MTF-1 promoter exhibited various transcription factor binding motifs that would be potentially related with metal regulation, stress responses, and development. The highest messenger RNA (mRNA) expression level of MTF-1 was observed in the testes, and MTF-1 transcripts were detected during the entire period of embryonic and early ontogenic developments. Abalone MTF-1 was found to be Cd inducible and highly modulated by heat shock treatment. Conclusion: Abalone MTF-1 possesses a non-consensus structure of activation domains and represents distinct features for its activation mechanism in response to metal overload and heat stress. The activation mechanism of abalone MTF-1 might include both indirect zinc sensing and direct de novo synthesis of transcripts. Taken together, results from this study could be a useful basis for future researches on stress physiology of this abalone species, particularly with regard to heavy metal detoxification and thermal adaptation.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.5 no.2
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• pp.17-23
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• 1999

Top-to-bottom compression strength of corrugated fiberboard boxes is partly dependent on the load-carrying ability of the central panel areas. The ability of these central areas to resist bending under load will increase the stacking strength of the box. The difference of box compression strengths, among boxes which are made with identical dimensions and fabricated with same components but different flute sizes, is primarily due to difference of the flexural stiffness of the box panels. Top-to-bottom compression strength of a box is accurately predicted by flexural stiffness measurements and the edge crush test of the combined boards. This study was rallied out to analyze the flexural stiffness, maximum bending force and maximum deflection for various corrugated fiber-boards by experimental investigation. There were significant differences between the machine direction (MD) and the cross-machine direction (CD) of corrugated fiberboards tested. It was about 50% in SW and DW, and $62%{\sim}74%$ in dual-medium corrugated fiberboards(e.g. DM, DMA and DMB), respectively. There were no significant differences of maximum deflection in machine direction among the tested fiberboards but, in cross direction, DM showed the highest value and followed by SW, DMA, DMB and DW in order. For the corrugated fiberboards tested, flexural stiffness in machine direction is about $29%{\sim}48%$ larger than cross direction, and difference of flexural stiffness between the two direction is the lowest in DMA and DMB.