• 한국발생생물학회지:발생과생식
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• 제17권1호
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• pp.63-72
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• 2013

A specific inhibitor of RNA polymerase II, ${\alpha}$-amanitin is broadly used to block transcriptional activities in cells. Previous studies showed that ${\alpha}$-amanitin affects in vitro maturation of cumulus-oocyte-complex (COC). In this study, we evaluated the target of ${\alpha}$-amanitin, and whether it affects oocytes or cumulus cells (CCs), or both. We treated ${\alpha}$-amanitin with different time period during in vitro culture of denuded oocytes (DOs) or COCs in comparison, and observed the changes in morphology and maturation status. Although DOs did not show any change in morphology and maturation rates with ${\alpha}$-amanitin treatment, oocytes from COCs were arrested at metaphase I (MI) stage and CCs were more scattered than control groups. To discover causes of meiotic arrest and scattering of CCs, we focused on changes of cumulus expansion, gap junctions, and cellular metabolism which to be the important factors for the successful in vitro maturation of COCs. Expression of genes for cumulus expansion markers (Ptx3, Has2, and Tnfaip6) and gap junctional proteins (Gja1, Gja4, and Gjc1) decreased in ${\alpha}$-amanitin-treated CCs. However, these changes were not observed in oocytes. In addition, expression of genes related to metabolism (Prps1, Rpe, Rpia, Taldo1, and Tkt) decreased in ${\alpha}$-amanitin-treated CCs but not in oocytes. Therefore, we concluded that the transcriptional activities of CCs for supporting suitable transcripts, especially for its metabolic activities and formation of gap junctions among CCs as well as with oocytes, are important for oocytes maturation in COCs.

• 한국전산구조공학회논문집
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• 제24권4호
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• pp.429-438
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• 2011

본 논문에서는 Membrane형 LNG선의 구성 요소를 대상으로 단열창의 열적 분포를 알아보기 위해 극저온 상태에서부터 온도 별로 각 소재의 열적 물성치인 열전도도(thermal conductivity)를 실험을 통해서 알아보았다. 극저온 상태인 $-163^{\circ}C$의 온도상태로 유지되어야 하는 LNG선 화물탱크는 단열재료로 하여금 열을 차단하기 위해 많은 연구가 되어야 하는데 특히 여러 재료로 구성되어 있는 단열 화물창(CCS: Cargo containment system)은 열적 물성치가 온도에 따라 각각 어떠한 값을 가지는 것이 주요 관심대상이고, 이를 통해 전체 LNG 단열 화물창이 어떤 열적 분포를 가지는 것에 대한 연구가 필요하다. 실험을 통해 얻은 물성치를 가지고 전체 화물창의 온도분포를 정적 열해석을 통해 알아보았다. 또한 외부의 충격에 의해 LNG가 누수되었을때 2차 방벽 특히 hull 부분에서는 누수량에 대해서 어떠한 온도분포와 열적 안전성에 대해서 알아보았다.

• 대한조선학회논문집
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• 제45권6호
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• pp.735-749
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• 2008

For the development of the original technique of structural safety assessment of Cargo Containment System(CCS) in membrane type LNG carriers, it is necessary to understand the characteristics of dynamic response behavior of CCS structure under sloshing impact pressure. In the previous study, the wet drop impact response analyses of CCS structure in membrane Mark III type LNG carriers were carried out by using Fluid-Structure Interaction(FSI) analysis technique of LS-DYNA code, and were also validated through a series of wet drop experiments for the enhancement of more accurate shock response analysis technique. In this study, the characteristics of structural shock response behaviors of CCS structure were sufficiently figured out by careful examinations of the effects of specimen weight, drop height, incident angle, corrugation and stiffness of inner hull on its shock response behaviors. The shock response analysis of upward shooting fluid to inner hull was performed, and the reason of faster strain response than shock pressure one was also figured out.

• 한국과학교육학회지
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• 제21권2호
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• pp.279-288
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• 2001

본 연구에서는 인지 갈등 유발 전략(CCS)과 사회적 합의 형성 전략(SCS) 하에서 진행된 소집단 토론의 상호작용 양상을 비교하였다. 과학 개념을 학습하는 4개 소집단 16명의 학생들을 대상으로 개별 진술, 상호작용 단위, 에피소드 수준에서 언어적 상호작용을 분석하였다. 토론 1회 당 전체 개별 진술이나 지식구성 진술은 CCS 집단에 비해 SCS 집단에서 많이 나타났다. 특히, 가설의 비교 평가나 토론 활동지는 SCS 집단 학생들의 메타인지적 진술을 증가시키는 데 효과적이었다. SCS 집단에서는 다수 참여형 및 정교화 상호작용이나 탐구형 에피소드의 빈도가 상대적으로 높아, 많은 학생들이 소집단 토론에 참여하였고 상호작용적인 정교화 토론이 진행된 것으로 나타났다. 상호작용이나 에피소드의 질적인 측면에서도 SCS 집단이 CCS 집단보다 우수하였다.

• 한국산학기술학회논문지
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• 제19권11호
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• pp.126-136
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• 2018

본 연구는 머신러닝을 활용하여 급성 뇌졸중 퇴원 환자의 중증도 보정 사망 예측 모형 개발을 목적으로 시행하였다. 전국 단위의 퇴원손상심층조사 2006~2015년 자료 중 한국표준질병사인분류(Korean standard classification of disease-KCD 7)에 따라 뇌졸중 코드 I60-I63에 해당하는 대상자를 추출하여 분석하였다. 동반질환 중증도 보정 도구로는 Charlson comorbidity index(CCI), Elixhauser comorbidity index(ECI), Clinical classification software(CCS)의 3가지 도구를 사용하였고 중증도 보정 모형 예측 개발은 로지스틱회귀분석, 의사결정나무, 신경망, 서포트 벡터 머신 기법을 활용하여 비교해 보았다. 뇌졸중 환자의 동반질환으로는 ECI에서는 합병증을 동반하지 않은 고혈압(hypertension, uncomplicated)이 43.8%로, CCS에서는 본태성고혈압(essential hypertension)이 43.9%로 다른 질환에 비해 가장 월등하게 높은 것으로 나타났다. 동반질환 중중도 보정 도구를 비교해 본 결과 CCI, ECI, CCS 중 CCS가 가장 높은 AUC값으로 분석되어 가장 우수한 중증도 보정 도구인 것으로 확인되었다. 또한 CCS, 주진단, 성, 연령, 입원경로, 수술유무 변수를 포함한 중증도 보정 모형 개발 AUC값은 로지스틱 회귀분석의 경우 0.808, 의사결정나무 0.785, 신경망 0.809, 서포트 벡터 머신 0.830로 분석되어 가장 우수한 예측력을 보인 것은 서포트 벡터머신 기법인 것으로 최종 확인되었고 이러한 결과는 추후 보건의료정책 수립에 활용될 수 있을 것이다.

• Korean Chemical Engineering Research
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• 제47권5호
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• pp.531-537
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• 2009

무분별한 화석연료 사용에 따른 온실가스 배출의 영향으로 지구 온난화 현상과 이와 연계된 환경 재해들이 발생하고 있다. 대표적 온실가스인 이산화탄소를 방출하지 않고 포집하여 저장하는데 관련된 일련의 기술들을 CCS(carbon dioxide capture and storage)라 하여 최근 매우 큰 주목을 받고 있다. CCS 기술 중에서 화학 흡수법(chemical absorption)은 대규모 처리가 가능하다는 측면에서 그 중에서 가장 많이 상용화된 기술이다. 하지만 발생하는 이산화탄소를 경제적으로 처리할 수 있기까지는 아직도 많은 부분들을 개선할 필요가 있다. 본 논문에서는 공정 시스템 공학 측면에서 화학 흡수법에 대하여 최근 연구 동향을 살펴보고 향후 연구 방향에 대해 살펴 보고자 한다.

• International Journal of Concrete Structures and Materials
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• 제4권2호
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• pp.89-96
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• 2010

This paper focuses on the effect of creep on the critical compressive strain (CCS) of concrete. The strain of concrete corresponding to the peak compressive stress is crucial in the selection of the ultimate yield strength of the reinforcing bar used in reinforced concrete columns. Among the various influencing factors, such as the creep, shrinkage, loading rate and confinement, the effect of creep and shrinkage is the most significant. So far, investigations into how these factors can affect the CCS of concrete have been rare. Therefore, to investigate the effect of creep and shrinkage on CCS, an experimental (part I) and a parametric study (part II) were conducted, as presented in these papers (part I considers creep effect, part II considers effect of creep and shrinkage). In part I, experiments pertaining to the loading age, loading rate, loading duration and loading and creep levels were conducted to study the effect of these variables on the CCS of concrete. It was found that the effects of the loading rate, loading age, and level and duration on the CCS of concrete were negligible. However, it is very important to consider the effect of creep.

• 신재생에너지
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• 제3권1호
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• pp.60-67
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• 2007

Catalyst coated membrane [CCM] type and catalyst coated substrate [CCS] type of membrane electrode assembly [MEA] were manufactured and evaluated their performance. Degradation test were conducted to find the difference of long term stability in two types of MEA and the factor for performance degradation problem occurred. Performance degradation test of single cell in two different types of MEA were carried out when current density was $200mA/cm^{2}$. The degradation test had proceeded for 230 hours and performance degradation was checked by I-V curve and impedance measurement at regular intervals. Also, MEA before/after operation and changes of catalyst layer were characterized by SEM, TEM, and XRD. Maximum power density of CCM type was higher than that of CCS type. Meanwhile, an increase of particle size of catalyst and an increase of impedance resistance after long term operation were observed. In the case of using CCM type MEA, the performance was deteriorated 38% of initial performance. In the case of using CCS type MEA, the performance was deteriorated 43% of initial performance. In consideration of difference of initial performance, performance of CCM type is higher than that of CCS type but both types had similar problems during degradation test.

• Asian-Australasian Journal of Animal Sciences
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• 제15권4호
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• pp.466-469
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• 2002

The data on 512 Hariana cows, progeny of 20 sires calved during period from 1974 to 1993 maintained at Government Livestock Farm, Hisar were considered for the estimation of genetic parameters. The means for first lactation milk yield (FLY), wet average (WA), first lactation peak yield (FPY), first lactation milk yield per day of first calving interval (MCI) and first lactation milk yield per day of age at second calving (MSC) were 1,141.58 kg, 4.19 kg/day, 6.24 kg/day, 2.38 kg/day and 0.601 kg/day, respectively. The effect of period of calving was significant (p<0.05) on WA, FPY and MCI while the effect of season of calving was significant only on WA. Monsoon calvers excelled in performance for all the production efficiency traits. The effect of age at first calving (linear) was significant on all the traits except on MCI. Estimates of heritabilty for all the traits were moderate and ranged from 0.255 to 0.333 except for WA (0.161). All the genetic and phenotypic correlations among different production efficiency traits were high and positive. It may be inferred that selection on the basis of peak yield will be more effective as the trait is expressed early in life and had reasonably moderate estimate of heritability.

• 한국대기환경학회지
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• 제28권1호
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• pp.52-58
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• 2012

In this study, the effect of standard phase difference in calibration of carbonyl compounds (CC) was evaluated by using their standards prepared in both gaseous and liquid phase. For this analysis, standards in both phases were prepared for 6 different CCs (formaldehyde (FA), acetaldehyde (AA), propionaldehyde (PA), butyraldehyde (BA), isovaleraldehyde (IA) and valeraldehyde (VA)) at similar concentration levels. Their gaseous standard was calibrated after derivatization with three types of DNPH cartridge, and their calibration results were compared against liquid-phase standards. Although there was a strong compatibility between 2 phases for CCs with lower molecular weights (e.g., formaldehyde and acetaldehyde), it was not the case for the heavier CCs. The results of our analysis indicate that the analytical bias of the heavier CCs can be significantly large (by more than a few tens of %). As a result, underestimation of hevier CCs can be significant, if their gaseous samples are quantified by liquid phase standard.