• 제목/요약/키워드: CAPS

검색결과 332건 처리시간 0.022초

Marker Development for Erect versus Pendant-Orientated Fruit in Capsicum annuum L.

  • Lee, Heung-Ryul;Cho, Myeong-Cheoul;Kim, Hyoun-Joung;Park, Sung-Woo;Kim, Byung-Dong
    • Molecules and Cells
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    • 제26권6호
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    • pp.548-553
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    • 2008
  • The erect habit of fruit setting is a unique characteristic of ornamental peppers and wild pepper species. The erect habit is known to be controlled by the up locus on pepper (Capsicum annuum L.) chromosome 12. The result of a genetic analysis using Saengryeog 211 (pendant), Saengryeog 213 (erect), and their $F_1$ and $BC_1$ progeny demonstrated that up is a recessive gene. To develop an up-linked marker, bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) were employed using 108 $F_{2:3}$ individuals. The closest AFLP marker, $A2C7_{469}$, was located at a genetic distance of 1.7 cM from the up locus and was converted into a cleaved amplified polymorphic sequence (CAPS) marker. This marker was mapped at a genetic distance of 4.3 cM from the up locus. When the CAPS was applied to seven ornamental lines and 27 breeding lines with erect fruit, these genotypes of 28 lines were correctly predicted. Thus, the CAPS marker will be useful for marker-assisted selection (MAS) of pepper breeding lines with the up allele at the early seedling stage.

임상가용 아동 및 청소년을 위한 외상 후 스트레스장애 척도를 통한 학교폭력 피해아동의 인지행동집단치료 효과 : 예비연구 (The Effects of Cognitive-Behavioral Group Therapy on Victims of School Violence as Assessed by the Clinician-Administered PTSD Scale for Children and Adolescents (CAPS-CA) : A Preliminary Study)

  • 김형욱;김태형;은헌정;최말례;권태완;구정일;조수진;송옥선
    • 대한불안의학회지
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    • 제3권2호
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    • pp.97-103
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    • 2007
  • Objective : The present study was carried out in order to assess the effects of Cognitive-Behavioral Group Therapy (CBGT) on victims of school violence, as assessed by the CAPS-CA. Methods : The subjects were 14 children who had experienced school violence and were subsequently diagnosed with PTSD and partial PTSD using the Clinician-Administered PTSD scale for Children and Adolescents (CAPS-CA). Seven children agreed to participate in CBGT, and they received 10 sessions of therapy. After CBGT, both the CBGT and non-CBGT groups were assessed using the CAPS-CA and School Adaptation Test. Results : The study results showed significant decreases in avoidance (p=0.010) and hyperarousal in the CBGT group (p=0.009) following the completion of CBGT. The analysis of school adaptation showed that there was a significant improvement in peer relationships after CBGT in the CBGT group (p=0.045). Conclusion : CBGT is effective in improving PTSD symptoms in children who experience school violence and subsequently suffer from PTSD.

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표고버섯 품종 '산마루2호'를 구분할 수 있는 CAPS marker 개발 (Development of a CAPS marker for the identification of the Lentinula edodes cultivar, 'Sanmaru 2ho')

  • 문수윤;이화용;가강현;구창덕;류호진
    • 한국버섯학회지
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    • 제16권1호
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    • pp.51-56
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    • 2018
  • 우리나라에서 표고버섯은 소비자의 선호도가 매우 높고, 전체 임산버섯 생산량의 약 97.7%를 차지하고 있는 매우 중요한 단기소득임산물중 하나이다. 이러한 표고버섯은 최근 신품종의 개발이 활발히 이루어지고 있어, 육종가의 권리 보호를 위하여 품종을 구분할 수 있는 분자마커 개발이 요구되고 있다. 본 연구에서는 신품종 '산마루2호'를 37개의 표고버섯 품종으로부터 구분할 수 있는 CAPS 마커를 개발하였다. '산마루2호'의 유전체에서 Scaffold 2번 1803483에 위치한 단일염기 다형성(SNP)이 확인되었고, 이 SNP를 포함하여 증폭한 DNA는 제한효소 Hhal에 의하여 특이적으로 절단되지 않아 다른 표고버섯 품종들과 구분되었다.

볼트 체결부 도난방지 안전캡 드로잉금형의 불량과 대책에 관한 연구 (A study on the defects of drawing dies for anti-theft safety caps for bolted joints and on problem-solving measures)

  • 김세환;최계광
    • Design & Manufacturing
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    • 제6권1호
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    • pp.79-83
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    • 2012
  • Anti-theft safety caps for bolted joints are necessary to ensure the safety of citizens and prevent theft of manholes, bridge guardrails, bulletin boards, subway safety handrails and others. Safety caps enhance the look of bolted joints, avert accidents that involve hitting the head of bolts, hamper them from coming loose by covering the head of bolts. This study focuses on press die design methods and press molding condition setting to resolve bursting and cracks in products, and on molding material selection and heat treatment technologies to boost life extension.

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강재결합구를 이용한 강관말뚝 합성형 두부결합 방법 (Composite Bonding Methods of Steel Pipe Piles into Pile Caps Using Steel Couplers)

  • 오성남;심창수;유재남;홍성영
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2001년도 봄 학술발표회 논문집
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    • pp.323-330
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    • 2001
  • The existing bonding methods of steel pipe piles into pile caps have many problems during construction or design. To overcome these, the composite bonding method of the bolting type and the welding type are proposed in this stud\ulcorner. The full scale test and the numerical analysis using finite element method were performed to verify the function of them. As results, the method of the filled with concrete in steel pipe piles head was good effective to increse strength. And the composite bonding methods are effective to protect the damage caused by earthquake than the bolted bonding method. Also, the composite bonding methods are cheaper than the existing bonding methods and a good construction as well.

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RAI(Resident Assessment Instrument)를 이용한 노인가정간호 및 재가복지서비스 정보시스템 개발 (Development of a Database System for Home Care Service Based on RAI (Resident Assessment Instrument))

  • 전경자;김은영
    • 지역사회간호학회지
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    • 제14권1호
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    • pp.75-82
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    • 2003
  • The purpose of this study was to develop an information system for home care service based on RAI(Resident Assessment Instrument). The standardization of service providing process was conducted using the steps of need assessment, triggers, application of CAPs, and care plan. The structure of MDsoft-HC was composed by MDS-CAPS system and system management system. A database on home care clients was accumulated by putting data, respectively, in general information, MDS-item, and MDS-result. Based on this data, the list of CAPs for the client was selected and monthly and annual statistics were calculated by problem result counts. It was suggested that standardization of a care plan would be integrated and short form of need assessment would be developed in the next stage.

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Characterization of F2 Progenies of Wound Minus Arabidopsis Mutant Crossed with Wild Type Plant

  • Park, Sanggyu
    • Journal of Applied Biological Chemistry
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    • 제43권1호
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    • pp.12-17
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    • 2000
  • To understand the signal transduction pathway that leads to the activation of the wound-inducible proteinase inhibitor II (pin2) promoter. $F_2$ progenies of wound (-) mutant crossed with wild-type Arabidopsis plants were biochemically and genetically characterized. Wound (-) mutant was derived from transgenic Arabidopsis plants containing bacterial cytosine deaminase gene under the control of pin2 promoter. The cytosine deaminase assays indicated that wound (-) mutant is a dominant inhibitor of wound-inducibility as only 3 of the $20F_2$ progenies showed cytosine deaminase (CDase) activity, To construct a structural map of the wound (-) mutant chromosomal regions, cleaved, amplified polymorphic sequences (CAPS) markers that cover all Chromosomes were used. Chromosomal regions covered by three different CAPS markers could be candidates for further fine mapping of the location of the wound (-) mutation. g4026, RGA1 and ASA1 located at 84.9 on recombinant inbred (RI) map of chromosome I, at 1.75 on RI map of chromosome II, and 18.35 on RI map of chromosome V, respectively.

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Single Nucleotide Polymorphisms linked to the SlMYB12 Gene that Controls Fruit Peel Color in Domesticated Tomatoes (Solanum lycopersicum L.)

  • Kim, Bichsaem;Kim, Nahui;Kang, Jumsoon;Choi, Youngwhan;Sim, Sung-Chur;Min, Sung Ran;Park, Younghoon
    • 원예과학기술지
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    • 제33권4호
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    • pp.566-574
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    • 2015
  • Yellow or transparent fruit peel color is caused by the accumulation or lack of naringenin chalcone (NG, C) in fruit peel and determines the red or pink appearance of tomato fruit, respectively. NGC biosynthesis is regulated by the SlMYB12 gene of the Y locus on chromosome 1, and DNA markers derived from SlMYB12 would be useful for marker-assisted selection (MAS) of tomato fruit color. To develop a gene-based marker, 4.9 kb of the SlMYB12 gene including a potential promoter region was sequenced from the red-fruited (YY) line 'FCR' and pink-fruited (yy) line 'FCP'. Sequence alignment of these SlMYB12 alleles revealed no sequence variations between 'FCR' and 'FCP'. To identify SlMYB12-linked single nucleotide polymorphisms (SNPs), 'FCR' and 'FCP' were genotyped using a SolCAP Tomato SNP array and CAPS markers (CAPS-456, 531, 13762, and 38123) were developed from the four SNPs (solcap_snp_sl_456, 531, 13762, and 38123) most closely flanking the SlMYB12. These CAPS markers were mapped using $F_2$ plants derived from 'FCR' ${\times}$ 'FCP'. The map positions of the fruit peel color locus (Y) were CAPS-13762 (0 cM) - 456 (11.09 cM) - Y (15.71 cM) - 38123 (17.82 cM) - 531 (30.86 cM), and the DNA sequence of SlMYB12 was physically anchored in the middle of CAPS-456 and CAPS-38123, indicating that fruit peel color in domesticated tomato is controlled by SlMYB12. A total of 64 SolCAP tomato germplasms were evaluated for their fruit peel color and SNPs located between solcap_snp_sl_456 and 38123. Seven SNPs that were detected in this interval were highly conserved for pink-fruited accessions and specific to transparent fruit peel traits, as depicted by a phenetic tree of 64 accessions based on the seven SNPs.

표고버섯 품종 산마루1호, 천장3호를 구분할 수 있는 CAPS Marker 개발 (Development of Cleaved Amplified Polymorphic Sequence Markers for the Identification of Lentinula edodes Cultivars Sanmaru 1ho and Chunjang 3ho)

  • 문수윤;이화용;김명길;가강현;고한규;정종욱;구창덕;류호진
    • 한국균학회지
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    • 제45권2호
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    • pp.114-120
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    • 2017
  • 표고버섯은 주로 아시아 국가에서 재배되는 식용 버섯이다. 표고버섯은 최근 국내에서 신품종의 개발이 활발히 이루어지고 있으며, 국내외적으로 품종의 보호가 중요해짐에 따라 표고의 품종을 구분할 수 있는 효율적인 마커 개발이 요구되고 있다. 본 연구에서는 산마루1호와 천장3호를 구분할 수 있는 CAPS (cleaved amplified polymorphic sequence) 마커를 개발하였다. 이 연구에서 개발된 CAPS 마커는 단핵균주인 B17의 표준유전체 정보와 연구에 사용된 10개 균주의 resequencing 정보를 바탕으로 개발되었다. 산마루1호는 scaffold9번, 1630048의 염기서열 G가 T로 변한 SNP를 포함하여 PCR 후 제한효소 TspR I을, 천장3호는 scaffold13번, 920681의 염기서열 G가 A로 변한 single nucleotide polymorphism (SNP)를 포함하여 PCR 후, 제한효소 Xho I을 처리하였을 때 다른 균주들과 구분되었다. 따라서 이를 마커로 개발하였다.