The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Development of Cleaved Amplified Polymorphic Sequence Markers for the Identification of Lentinula edodes Cultivars Sanmaru 1ho and Chunjang 3ho

표고버섯 품종 산마루1호, 천장3호를 구분할 수 있는 CAPS Marker 개발

  • Moon, Suyun (Department of Biology, Chungbuk National University) ;
  • Lee, Hwa-Yong (Department of Biology, Chungbuk National University) ;
  • Kim, Myungkil (Wood Chemistry and Microbiology Division, National Institute of Forest Science) ;
  • Ka, Kang-Hyeon (Wood Chemistry and Microbiology Division, National Institute of Forest Science) ;
  • Ko, Han Kyu (Forest Mushroom Research Center, National Forestry Cooperative Federation) ;
  • Chung, Jong-Wook (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Koo, Chang-Duck (Department of Forest Science, Chungbuk National University) ;
  • Ryu, Hojin (Department of Biology, Chungbuk National University)
  • 문수윤 (충북대학교 생물학과) ;
  • 이화용 (충북대학교 생물학과) ;
  • 김명길 (국립산림과학원 화학미생물과) ;
  • 가강현 (국립산림과학원 화학미생물과) ;
  • 고한규 (산림조합중앙회 산림버섯연구센터) ;
  • 정종욱 (충북대학교 특용식물학과) ;
  • 구창덕 (충북대학교 산림학과) ;
  • 류호진 (충북대학교 생물학과)
  • Received : 2017.03.07
  • Accepted : 2017.04.19
  • Published : 2017.06.01
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Abstract

Lentinula edodes is an edible mushroom that is mainly cultivated in Asian countries. Recently, new cultivars of this mushroom have been developed in Korea; variety protection is very important, so the development of efficient molecular markers that can distinguish each variety is required. In this study, we developed cleaved amplified polymorphic sequence (CAPS) markers for the identification of L. edodes cultivars (Sanmaru 1ho and Chunjang 3ho). These markers were developed from whole genomic sequencing data from L. edodes monokaryon strain B17 and resequencing data from 10 dikaryon strains. A single nucleotide polymorphism changed in scaffold 9 POS 1630048 in Sanmaru 1ho($G{\rightarrow}T$), and in scaffold 13 POS 920681 in Chunjang 3ho ($G{\rightarrow}A$). The restriction enzymes TspR I and Xho I distinguished Sanmaru 1ho and Chunjang 3ho, respectively, from other strains. Thus, we developed 2 CAPS markers for the identification of the L. edodes cultivars Sanmaru 1ho and Chunjang 3ho.

표고버섯은 주로 아시아 국가에서 재배되는 식용 버섯이다. 표고버섯은 최근 국내에서 신품종의 개발이 활발히 이루어지고 있으며, 국내외적으로 품종의 보호가 중요해짐에 따라 표고의 품종을 구분할 수 있는 효율적인 마커 개발이 요구되고 있다. 본 연구에서는 산마루1호와 천장3호를 구분할 수 있는 CAPS (cleaved amplified polymorphic sequence) 마커를 개발하였다. 이 연구에서 개발된 CAPS 마커는 단핵균주인 B17의 표준유전체 정보와 연구에 사용된 10개 균주의 resequencing 정보를 바탕으로 개발되었다. 산마루1호는 scaffold9번, 1630048의 염기서열 G가 T로 변한 SNP를 포함하여 PCR 후 제한효소 TspR I을, 천장3호는 scaffold13번, 920681의 염기서열 G가 A로 변한 single nucleotide polymorphism (SNP)를 포함하여 PCR 후, 제한효소 Xho I을 처리하였을 때 다른 균주들과 구분되었다. 따라서 이를 마커로 개발하였다.

Keywords

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