• Journal of Plant Biotechnology
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• v.37 no.2
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• pp.174-185
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• 2010

Full-length cDNAs are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. To elucidate the functions of a large population of Chinese cabbage (Brassica rapa) genes and to search efficiently for agriculturally useful genes, we have been taking advantage of the full-length cDNA Over-eXpresser (FOX) gene hunting system. With oligo dT column it purify the each mRNA from the flower organs, leaf and stem tissue. And about 120,000 cDNAs from the library were transformed into $\lambda$-pFLCIII-F vector. Of which 115,000 cDNAs from the library were transformed into T-DNA binary vector, pBigs for transformation study. We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. Full-length Chinese cabbage cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Selfed seeds were harvested from transgenic Arabidopsis. We had selected 2,500 transgenic plants by hygromycin antibiotic tolerant test, and obtained a number of transgenic mutants. Each transgenic Arabidopsis was investigated in morphological changes, fertility and leaf colour. As a result, 285 possible morphological mutants were identified. Introduced cDNA was isolated by PCR amplification of the genomic DNA from the transgenic mutants. Sequencing result and BLAST analysis showed that most of the introduced cDNA were complete cDNAs and functional genes. Also, we examined the effect of Bromelain on enhancing resistance to soft rot in transgenic Chinese cabbage 'Osome'. The bromelain gene identified from FOX hunting system was transformed into Chinese cabbage using Agrobacterium methods. Transformants were screened by PCR, then RT-PCR and real time PCR were performed to analyze gene expression of cysteine protease in the T1 and T2 generations. The anti-bacterial activity of bromelain was tested in Chinese cabbages infected with soft rot bacteria. The results showed that the over-expressed bromelain gene from pineapple conferred enhanced resistance to soft rot in Chinese cabbage.

• Korean Journal Plant Pathology
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• v.1 no.3
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• pp.157-164
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• 1985

Some interactions in various soil conditions, numbers of microbial populations, root rot disease development and rates of spore germiation in three different location of soils were investigated. The calcium and magnesium contents were higher in replanted fields of ginseng (Panax ginseng) at Goesan. Potassium contents were high in replanted field at Poonggi and textural class of the soils was silt loam except for silt clay loam in first cultured field of ginseng at Goesan. For the germination process of Fusarium solani, F. moniliforme, F. oxysporum, and Alternaria panax, the percentage germination of fungal spores was high in double distilled water and Pfeffer's solution as media, whereas the lower rate of germination of spores was observed in soil extracts. Numbers of bacteria were high in replanted field soil at Gumsan, and propagules of fungi in replanted fields at Gumsan and Poonggi were higher than other soils, but higher numbers of actinomycetes were found in the first cultured field of ginseng at Goesan and Poonggi. Fungistasis was induced by higher microbial populations present in soil that was initiated when amended with garlic stalk, crushed bean and ginseng leaves. On the other hand, there was no fungistasis in soil amended with wheat and barley straw, and this tendency was a little difference on the soil sample.

• Proceedings of the Korea Society for Industrial Systems Conference
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• 2000.11a
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• pp.575-585
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• 2000

본 사업은 1994년부터 1996년까지 3년 간 시범사업으로 추진된 지역정보화 사업의 과제인 창원/마산 지역정보 유통망 구축사업을 확대 발전시키고 시스템 운영의 효율화를 도모하고자 DB를 통합하여 확대 재 구축함으로써 전자상거래 기반의 인터넷을 통한 웹 정보 이용 활성화 차원에서 추진되었다. 기 구축되었던 $\ulcorner$창원/마산 지역정보유통망 구축사업$\lrcorner$은 정보화를 통한 지역경제 발전에 일익을 담당하고자 관. 산. 연이 참여한 지역정보화 사업이다. 이러한 창원/마산지역 산업체에 대한 지원효과를 이번에 확대하여 그 동안 Multimedia, Graphics, Web 등 정보화 환경이 크게 변화, 발전됨에 따라 현실적이며 현장적용형 패키지의 개발 지원이 대두된 $\ulcorner$경남지역 정보화확산사업$\lrcorner$은 연구개발정보센터와 창원시, 진해시, 한국기계연구원이 매칭 펀드에 의한 대응자금으로 구축한 시스템이다. 정보수집대상은 경남지역에 소재하고 있는 20개 산업단지 3,700여 입주기업을 정보수집요원이 직접 방문하여 현장에서 필요로 하는 요구정보를 반영하였다. 신규로 구축한 정보는 산업기반 기업체기본정보 DB 및 기업 기술개발 현장적용형 기술정보 패키지정보를 중심으로 구축하였으며, 인터넷 활용교육을 통한 동종 업종간 정보교류체제 구축하였다. 특히 Cyber Market을 구현하기 위해 1,200여건의 기업체정보 및 제조상품 DB를 응용한 제품 Mall 데이터베이스를 구축하였다. 본 사업을 통하여 경남지역 산업단지에 입주한 기업체의 정보 활용을 극대화하여 지역경제 발전에 기여함과 동시에 국내 지역정보화 시범모델로서 위상을 확립하고자 한다.을 기업의 타인자본비용과 자기자본비용의 조합인 기회자본비용으로 할인함으로써 현재의 기업가치를 구할 수 있기 때문이다. 이처럼 기업이 영업활동이나 투자활동을 통해 현금을 창출하고 소비하는 경향은 해당 비즈니스 모델의 성격을 규정하는 자료도로 이용될 수 있다. 또한 최근 인터넷기업들의 부도가 발생하고 있는데, 기업의 부실원인이 어떤 것이든 사회전체의 생산력의 감소, 실업의 증가, 채권자 및 주주의 부의 감소, 심리적 불안으로 인한 경제활동의 위축, 기업 노하우의 소멸, 대외적 신용도의 하락 등과 같은 사회적·경제적 파급효과는 대단히 크다. 이상과 같은 기업부실의 효과를 고려할 때 부실기업을 미리 예측하는 일종의 조기경보장치를 갖는다는 것은 중요한 일이다. 현금흐름정보를 이용하여 기업의 부실을 예측하면 기업의 부실징후를 파악하는데 그치지 않고 부실의 원인을 파악하고 이에 대한 대응 전략을 수립하며 그 결과를 측정하는데 활용될 수도 있다. 따라서 본 연구에서는 기업의 부도예측 정보 중 현금흐름정보를 통하여 '인터넷기업의 미래 현금흐름측정, 부도예측신호효과, 부실원인파악, 비즈니스 모델의 성격규정 등을 할 수 있는가'를 검증하려고 한다. 협력체계 확립, ${\circled}3$ 전문인력 확보 및 인력구성 조정, 그리고 ${\circled}4$ 방문보건사업의 강화 등이다., 대사(代謝)와 관계(關係)있음을 시사(示唆)해 주고 있다.ble nutrient (TDN) was highest in booting stage (59.7%); however no significant difference was found among other stages. The concentrations of Ca and P were not different

• Food Science of Animal Resources
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• v.25 no.3
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• pp.350-356
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• 2005

We examined the enhancement of thermotolerance for storage conferred on Lactobacillus acidophilus 30SC by adaptation to different stresses. The viable cells of Lactobacillus acidophilus 30SC were compared with their viability prior to heating at $45,\;55^{\circ}C\;and\;60^{\circ}C$. Heat-adapted ($45^{\circ}C$ for 15 min) L. acidophilus 30SC in MRS broth exhibited higher survivability at lethal temperature of $55^{\circ}C$ than control. Cellular protein profiles of L. acidophilus 30SC during heat adaptation were examined with SDS-PAGE, and scanning electron microscopy. When L. acidophilus 30SC was heat-adapted at $55^{\circ}C$ for 15min, 5 new protein spots of ca $8\~45\;kDa$ size were observed on 2D SDS-PAGE. It was presumed that new proteins of L. acidophilus 30SC were produced to adapt to the environment of higher growth temperature.

• Korean Journal of Environmental Agriculture
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• v.35 no.4
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• pp.235-240
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• 2016

BACKGROUND: To determine effect of phosphate (P) application on Cadmium (Cd) extractability and its uptake by rice plant in Cd contaminated paddy soil, dipotassium ($K_2HPO_4$) which was the most effective of P materials to decrease Cd extractability in previous study was selected as P fertilizer. METHODS AND RESULTS: Dipotassium phosphate was applied at the rates of 0, 78, 234, and 390 kg $P_2O_5/ha$, and then rice was cultivated in submerged paddy soil from Jun. to Oct. in 2015. Cadmium concentrations in grain, straw, and root of rice plant decreased significantly with increasing application rate of $K_2HPO_4$. The trend of 1 M $NH_4OAc$ extractable Cd concentration in soil was similar to that of Cd uptake by rice plant. One M $NH_4OAc$ extractable Cd concentration was negatively related to soil pH and negative charge. Alleviation of Cd phytoavailability of rice in paddy soil might be attributed to increase in pH and negative charge of soil. Using a quadratic response model, amount of grain yield were related to $K_2HPO_4$ application rates as Grain yield = $5.38+2.39{\times}10^{-3}K_2HPO_4-6.65{\times}10^{-6}K_2HPO{_4}^2$ (model $R^2=0.968$). Using this equations, the greatest grain yield (5.6 Mg/ha) was at the rate of 180 kg $P_2O_5/ha$. At this application rate of P, the Cd concentration in grain was 0.53 mg/kg, implying ca. 23% lower than the control. CONCLUSION: From the view point of heavy metal safety and crop productivity, it might be good P management to apply P fertilizer with 4 times higher rate than recommendation (45 kg/ha).

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.2
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• pp.133-138
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• 1996

To investigate the effects of inorganic phosphate ($P_2O_5$ 200kg/ha), fermented cow manures($P_2O_5$ 200kg/ha, 400kg/ha) and poultry manures($P_2O_5$ 200kg/ha, 400kg/na) levels mixed with sawdust on the soil changes and productivity of alfalfa monoculture, a field experiment arranged by randonmized complete block design was conducted at National Livestock Research Institute in Suwon, September, 1993 to December 1995. The results are as follows. S1. Soil pH was changed from 5.40(before experiment) to 6.17(fermented cow manure, $P_2O_5$ 400kg/ha), 6.76 (fermented poultry manure, $P_2O_5$400kg/ha) and 6.3l(inorganic phosphate, $P_2O_5$ 200kg/ha) at 0~5cm depth of subsoil, respectively. Organic contents after experiment were lower as a range of 2.41%~3.15% than those before experiment. 2. Total nitrogen contents of each treatment showed as 0.13%~0.16% at 0~5cm depth of subsoil comparing with before experiment(0.15%). Available phosphate content of inorganic phosphate($P_2O_5$200kgha) was highest as 405ppm. lowest as 125ppm(fermented poultry manure, $P_2O_5$ 200kg/ha) than before trial(205ppm). 3. K and Ca contents of after experiment were appeared to 0.22%~0.78% and 6.45%~9.45% comparing with before experiment(0.10% and 3.56%), respectively. 4. During two years, average DM yield of fermented cow rnanure($P_2O_5$ 400kg/ha) was highest as 11, 731kg/ha and lowest as 9, 017kg/ha at fermented poultry manure plot($P_2O_5$ 200kg/ha) with as sawdust, but no significant difference was found among treatment(P>0.05). 5. CP production, NEL and TDN yield of fermented cow manure($P_2O_5$ 400kg/ha) were highest as 2, 282kg/ha 69, 900kg/ha and 6, 392kg/ha, respectively, but no significant difference among treatment(P>0.05). 6. In inorganic contents of alfalfas, there was not show same tendency in accordance with treatments. As a results mentioned above, it was possible to use 200kg $P_2O_5/ha$ of fermented cow or poultry manure with sawdust instead of inorganic phosphae fertilizer.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.1
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• pp.19-26
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• 1997

To find out the residual effect of phosphate fertilization on botanical composition, mineral contents and mineral nutrient deprivation of pasture plants, this experiment was arranged as a randomized complete block design with six treatments(0-0, 50-50, 100-65, 150-65, 200-65 and 250-65kg $P_2O_5$/ha), those were composed of three P,O, fertilization level(0, 50 and 65kg P,OJha) after phosphate fertilization trial with six treatments(0, 50, 100, 150, 200 and 250kg $P_2O_5$/ha) from 1989 to 1992, and conducted at hilly land in Kwangju, Kyonggi Province 60m 1993 to 1994. The results obtained are summarized as follows: The botanical composition of pasture plants without phosphate fertilization was very poor. With increasing available phosphate of soil, the percentage of grasses and legumes were remarkably increased and weeds was decreased. With 150-65kg $P_2O_5$/ha application for two years, the average DM yield was composed of 80.6% grasses, 15.4% legume and 4.0% weeds, the percentage was similar to that of 200-65kg $P_2O_5$/ha. As available phosphate of soil increase, P, K and Mg content of pasture plants were increased, but Ca/P ratio was lowed and the other mineral contents tended not to be regular. The mineral nutrient contents deprived by DM yield was increased as available phosphate of soil was increased, but those were not different between 150-65 and 250-65kg $P_2O_5$/ha. However, mineral nutrient of $P_2O_5$,$K_2O$ and MgO were higher in 200-65 and 250-65kg $P_2O_5$/ha. Most of applied mineral elements were accumulated at a subsurface(0-2.5cm) of grasslands, and mineral tended to be decrease than that of soil chemical analysis before the experiment of residual effect of phosphate fertilization.

• The Journal of the Petrological Society of Korea
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• v.12 no.1
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• pp.32-52
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• 2003

The Mt. Baegyang in Busan, composed of sedimentary basement rocks (Icheonri Formation), andesite (lava), andesitic pyroclastic rocks, fallout tuff and tuffaceous sedimentary rocks, rhyolitic pyroclastic rocks, intrusive rocks (granite-porphyry, felsite, and biotite-granite) of Cretaceous age in ascending order. The volcanic rocks show a section of composite volcano which comprised alternation of andesitic lava and pyroclasitc rocks, rhyolitic pyrocalstic rocks (tuff breccia, lapilli tuff, fine tuff) from the lower to the upper strata. From the major element chemical analysis, the volcanic and intrusive rocks belong to calc-alkaline rock series. The trace element composition and REE patterns of volcanic and plutonic rocks, which are characterized by a high LILE/HFSE ratio and enrichments in LREE, suggest that they are typical of continental margin arc calc-alkaline rocks produced in the subduction environment. Primary basaltic magma might have been derived from partial melting of mantle wedge in the upper mantle under destructive plate margin. Crystallization differentiation of the basaltic magma would have produced the calc-alkaline andesitic magma. And the felsic rhyolitic magma seems to have been evolved from andesitic magma with crystallization differentiation of plagioclase, pyroxene, and hornblende.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.6
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• pp.1681-1684
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• 2005

Gastric cancer is the most common disease which is the highest genetic rate, chemotherapy is the only known way to treat, especially advanced gastric cancer. Although, it is the remedy, the harmful side-effects are an obstacle of the sustaining treatment and negative effect on immunologic ability of patients prologed. Consequently, it is surely necessary that effective control for sequele of anti-cancer treatment, and remedy which can improve the quality of patients life. 1, the writer, medicate patients, diagnose as gastric cancer in 4th stage, with Sohap-hyangwon and Chungwoolhyadamjen who complained of pain in sequele of anti-cancer treatment. The size of the metastastic liver tumor is decreasing in patients and the immunologic abilities are maintaining stable. This clinical cases are shown that the oriental medical care in long-term can improve the quality of life of the patients who has sequele of anti-cancer treatment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.3
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• pp.242-253
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• 1984

Four different levels of nitrogen were applied to 3 naked barley cultivars (Hordeum vulgare L.) Yonezawa, Baekdong and Yeongsanbori. Changes in physico-chemical properties were examined upon these treatments. Total protein content as well as glutelin increased as N level increased. Baekdong contained the highest protein content (13.7%) at N$_2$. Yonezawa and Baekdong contained more lysine than Yeongsanbori. Increased lysine was measured in all cultivars by increased nitrogen application. Among all amino acids proline was the highest in all cultivars, and its increase was paralled to the N levels. Magnesium and potassium were declined and calcium was increased with increased levels of nitrogen fertilizer. Ash content was decreased as nitrogen increased and as the plants have become matured. Three minutes pearling in Yonezawa was not sufficient in terms of water absorption ratio and whiteness. Sixty percent pearling rate in Yonezawa showed a higher absorption ratio and cooking whiteness than non-waxy Baekdong and Yeongsanbori. Gelatinization temperature was lower but maximum viscosity was higher in waxy than non-waxy barley.