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Performance Evaluation of Water Loss Management in Urban Areas Using Different Performance Indicators (여러 가지 지표를 이용한 전국도시의 물손실관리 수행능 비교평가)

  • Chung, Shin-Ho;Yu, Myong-Jin;Koo, Ja-Yong;Lee, Hwa-Kyoung
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.2
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    • pp.117-127
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    • 2006
  • Recently International Water Association(IWA) has proposed new performance indicators for water supply services and their successful applications are being reported continually. According to the various reports, the percentage indicators were suggested not to be used for performance comparison, especially where the targeted areas have great difference in consumption per service connection. But unfortunately in Korea the revenue water ratio, one of the percentage indicators is still being used to set up the goal of water-related administration and to compare the performances between systems. Therefore this study aimed to prove the inapplicability of the percentage indicators with nation-wide data and to suggest better performance indicators for more efficient water loss management. According to the result of the comparison of various performance indicators with conventional one, it is roved that percentage indicators ca not evaluate the performance efficiently where the local situations are significantly different. It is suggested that the better performance indicators such as real losses per service connection or ILI should be used to benchmark the performances of water suppliers on water loss management so thai the problems of water losses could be identified easily and recovered effectively.

Expression and Characterization of Calcium- and Zinc-Tolerant Xylose Isomerase from Anoxybacillus kamchatkensis G10

  • Park, Yeong-Jun;Jung, Byung Kwon;Hong, Sung-Jun;Park, Gun-Seok;Ibal, Jerald Conrad;Pham, Huy Quang;Shin, Jae-Ho
    • Journal of Microbiology and Biotechnology
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    • v.28 no.4
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    • pp.606-612
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    • 2018
  • The enzyme xylose isomerase (E.C. 5.3.1.5, XI) is responsible for the conversion of an aldose to ketose, especially xylose to xylulose. Owing to the ability of XI to isomerize glucose to fructose, this enzyme is used in the food industry to prepare high-fructose corn syrup. Therefore, we studied the characteristics of XI from Anoxybacillus kamchatkensis G10, a thermophilic bacterium. First, the gene coding for XI (xylA) was inserted into the pET-21a(+) expression vector and the construct was transformed into the Escherichia coli competent cell BL21 (DE3). The expression of recombinant XI was induced in the absence of isopropyl-thio-${\beta}$-galactopyranoside and purified using Ni-NTA affinity chromatography. The optimum temperature of recombinant XI was $80^{\circ}C$ and measurement of the heat stability indicated that 55% of residual activity was maintained after 2 h incubation at $60^{\circ}C$. The optimum pH was found to be 7.5 in sodium phosphate buffer. Magnesium, manganese, and cobalt ions were found to increase the enzyme activity; manganese was the most effective. Additionally, recombinant XI was resistant to the presence of $Ca^{2+}$ and $Zn^{2+}$ ions. The kinetic properties, $K_m$ and $V_{max}$, were calculated as 81.44 mM and $2.237{\mu}mol/min/mg$, respectively. Through redundancy analysis, XI of A. kamchatkensis G10 was classified into a family containing type II XIs produced by the genera Geobacillus, Bacillus, and Thermotoga. These results suggested that the thermostable nature of XI of A. kamchatkensis G10 may be advantageous in industrial applications and food processing.

Development of the Eggs, Larvae and Juveniles by Artificially-Matured Pacific Mackerel, Scomber japonicus in the Korean Waters (성숙 유도한 한국산 고등어 (Scomber japonicus)의 난발생과 자치어의 형태 발달)

  • Kim, Dae-Hyun;Kim, Dae-Jung;Yoon, Seong-Jong;Hwang, Hyung-Gue;Kim, Eung-Oh;Son, Sang-Gyu;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.41 no.6
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    • pp.471-477
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    • 2008
  • Development of egg, larvae and juveniles for the Pacific mackerel, Scomber japonicus are described following natural fertilization in the indoor tank of $25^{\circ}C$ water temperature. Following a routine hormone treatment technique for the brood stock, male and female mackerels were artificially matured by intramuscular injections of LHRHa at a dosage of $400{\mu}g/kg$ body weight (BW)+Domperidone at a dosage of $4{\mu}g/kg$ body weight (BW) to induce maturation in a separate aquarium and induced natural spawning. Fertilized eggs were ca. 1.0 mm in diameter; spherical in shape with a single oil globule; pelagic and non-adhesive. Hatching occurs 41 hours after fertilization at $23-24^{\circ}C$. The newly hatched larvae was 3.03 mm in average total length (ATL), the mouth and anus were not open, oil globule located in posterior end of yolk sac, and preanal length was 42.8% of TL. The larvae measuring 2.89 mm ATL, almost absorbed yolk sac and oil globule material in 2 days after hatching, in which the mouth and anus were open. Melanophores, branch or star in shape were observed on the top of head, peritoneal region and along the ventral contour. In 13 days after hatching, the larvae was 6.88 mm ATL, its posterior end of notochord began to flex upward, finfold of caudal fin appeared, jaw teeth were already formed. In 19 days after hatching, the larvae was 7.71 mm ATL completed only caudal fin rays (9+8), and preanal length was 49.4% of TL. In 37 days after hatching, the larvae was 27.4 mm ATL already completed all the fins, and preanal length was 59.9% of TL.

Removal of Ammonia-Nitrogen Contained in Landfill Leachate by Ammonia Stripping(I) (암모니아 탈기공정을 이용한 침출수의 암모니아성 질소제거(I))

  • Lee, Byung-Jin;Cho, Soon-Haing
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.10
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    • pp.1893-1904
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    • 2000
  • Nitrogen compounds are one of the major pollutants which cause eutrophication problems of the river or lake and red tides problems of the ocean. Currently available technologies for the removal of nitrogen compounds are mostly biological treatment. However, biological treatment is only effective for the wastewater which contains low concentration of nitrogen compounds. Leachate from solid waste landfill or industrial wastewater which contains high concentration of nitrogen can not be effectively treated by most of the currently available biological treatment technologies. With this connection. the objective of this study is to examine the applicability of ammonia stripping technology for the removal of high concentration of ammonia nitrogen compounds of the leachate from solid waste landfill. It can be concluded that ammonia stripping technology which was placed before the biological treatment process was very effective for the removal of high concentration of ammonium compounds. The chemical cost for the ammonia stripping was 16 percent higher than MLE process, so other methods like sludge recycling are needed for the reduction of operation cost. Further details are discussed in this paper.

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Transfer of Isolated Nuclei from Agrocybe aegerita Mycelia into Pleurotus florida Protoplasts (사철느타리버섯 원형질체내(原形質體內) 버들볏짚버섯 핵(核)의 전이(轉移))

  • Yoo, Young-Bok;Shin, Pyung-Gyun;Kim, Han-Kyoung;Byun, Myung-Ok;You, Chang-Hyun;Cha, Dong-Yeul;Chang, Kwon-Yawl
    • The Korean Journal of Mycology
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    • v.17 no.3
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    • pp.114-118
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    • 1989
  • The transfer of isolated nuclei from Agrocybe aegerita mycelia of wild type into Pleurotus florida protoplasts of auxotroph was induced with polyethylene glycol. The type 1 of nuclear transfer products was spontaneous segregants of both parental morphology of colony. Hyphae of A. aegerita type had clamp connections while that of P. florida type lacked. Type 2 was main products of nuclear transfer which formed true clamp connections. Type 3 was clampless products. They all produced primordia and developed basidiocarps similar to Agrocybe aegerita. A comparison of nuclei transfer products was made using isozyme analyses of alcohol dehydrogenase, esterase, lactate dehydrogenase and peroxidase. Isozyme band patterns of some type 2 strains produced a new band. Band patterns from mycelial extracts of the other strains could be characterized by parental bands.

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The Approach of Properties-Flavours Theory and the Study of Morphological Standard in CORNI FRUCTUS (산수유(山茱萸)의 기미론(氣味論)적 해석과 내외부형태연구)

  • Choi, Mun-Il;Lee, Hwa-Jung;Yun, Ju-Bong;Kim, Ja-Young;Kang, Kyoung-Sik;Shin, Chol-Gyun;Ju, Young-Sung
    • The Korea Journal of Herbology
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    • v.21 no.2
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    • pp.109-119
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    • 2006
  • Objectives : This study was performed to approach of properties-flavours theory and the study of morphological standard in CORNI FRUCTUS. Methods : The properties-flavours theory were attempted with bibliographic method and various climate-information. The external-internal morphological standards were determined by using stereoscope and butanol series. Results and conclusion : 1. The significant analysis for the corni fructus as the properties-flavours theory, is a follow-up survey of the effect written in the official oriental medicine book. 2. The other analytic methods according to the gathering time, processing or storing system or especially nature or growth environment(altitude, temperature,. the mean moisture, and the agronomical survey) by way of the explanation of properties-flavours theory appears so many insufficiency at the many cases. 3. The external and internal characteristics according to the shape of original plant or herbal states, entirely correspond to all the literature on this subject. 4. According to the place of production, there are some different points. (l) In the external shapes, it is reasonable that if we make the basic standard of classification by the sharpness of color, the existence of gloss and the size of products. In the case of the Korean products, it shows bright and glossy color totally, but in the case of North Korea products, it shows black and lackluster. And in the case of the foreign products, it has softer body than Korea one and shows white color on the surface. (2) In the internal shapes, the Korea products has a thicker cuticle layer than the foreign one, and has fewer ca-oxalate in the parenchyma cell.

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Clinical, Laboratory Biomarkers and Imaging Findings of Pancreatic Adenocarcinoma in Iran

  • Hadizadeh, Mohammad;Padashi, Maryam;Alizadeh, Amir Houshang Mohammad;Zali, Mohammad Reza
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.10
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    • pp.4349-4352
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    • 2014
  • Background: Pancreatic cancer has a high mortality rate because it is usually diagnosed late. Since little is known about this cancer in Iran, with the aim of improving this knowledge deficiency, we evaluated clinical, laboratory biomarkers, imaging findings and treatment modalities in Iranian patients with pancreatic adenocarcinoma. Materials and Methods: 131 cases of pancreatic adenocarcinoma in 2010-2013 were obtained from the Taleghani Hospital Record Department. Cases confirmed by histopathology from CT-guided biopsy, EUS-FNA and surgery examination were included. We excluded those with incomplete medical records. Results: The study included 131 subjects between 24 and 97 years of age and a mean age of $63{\pm}13.4$ years. Eighty (61.1%) were male and 51 (38.9%) female. Previous history included diabetes mellitus in 36 (27.5%), alcohol drinking in 5 (3.9%), smoker in 28 (21.4%) and opium addiction in 13 (10%). The common presenting history included weight loss in 79 (60.3%), abdominal pain in 77 (58.8%), fever in 11 (8.4%), nausea in 30 (22.9%), jaundice in 72 (55%), pruritus in 52 (39.7) and anemia in 33 (25.2%). CA19-9 levels with cut offs of 50, 100 and 200 U/ml were increased in 81%, 72% and 66% of patients, respectively. Tumor staging was: stage I, 3 (2.3%); stage II, 10 (7.6%); stage III, 58 (44.3%); and stage IV, 60 (45.8%). From 45 patients, 17 received ERCP inserted metallic stents and 22 plastic stents, the remaining 6 failed that PTC was done. Whipple surgery and chemotherapy were conducted for 10 and 29 patients, respectively. Conclusions: This disease affected older people and there was a male preponderance. The commonest risk factors were diabetes mellitus, smoking and cholelithiasis. The majority of patients presented with loss of appetite, loss of weight, jaundice, abdominal pain and discomfort. Almost all presented at late stages of the disease so that curative surgery was impossible. Also chemotherapy was only performed in a few patients as a neoadjuant treatment.

Monitoring of Soil Chemical Properties and Pond Water Quality in Golf Courses after Application of SCB Liquid Fertilizer (골프코스에서 SCB저농도액비 살포에 따른 토양화학성과 연못수질의 모니터링)

  • Kim, Young-Sun;Ham, Suon-Kyu;Lim, Hye-Jung
    • Asian Journal of Turfgrass Science
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    • v.26 no.1
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    • pp.44-53
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    • 2012
  • As SCB liquid fertilizer (SCB) produced from or out of livestock manure by slurry composting and biofiltration process was applied in golf course, the effect on soil properties and water quality was little investigated. This study was conducted to evaluate the effect of the SCB liquid fertilizer application on environment by monitoring chemical property of soil and water quality of pond as applied chemical fertilizer (CF) and SCB. SCB application rarely contaminated the soil and pond in golf course and decreased organic matter, CEC and Ca in soil and pH and T-N for water quality of pond. In correlation coefficient between soil property parameters, water quality parameters and water quality items, SCB applied in golf course decreased organic matter and CEC in soil and increased SAR in water quality (P<0.01). Nitrogen applied in golf course with SCB or CF was significantly related to T-N in the soil (P<0.01), but not significantly related to T-N in the pond water. These results showed that SCB application little contaminated soil and pond in golf course, and was expected to control of thatch in soil and algae in pond.

Application of Poultry Industry Using Methods of Environmental Management - A Study on Decreasing Soluble Metals from Poultry Litter with Chemical Additives - (환경경영 기법을 이용한 가금산업에 적용(I) - 화학제재를 첨가한 깔짚으로부터 수용성 중금속 저감에 관한 연구를 중심으로 -)

  • Choi, In-Hag;Choi, Sun;Choi, Jung-Hoon
    • Journal of Environmental Science International
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    • v.18 no.12
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    • pp.1437-1442
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    • 2009
  • Recent studies have shown that alum addition to litter results in many environmental and economic advantages, such as reductions in metal runoff, lower ammonia emission and improved poultry performance. However, no research has been conducted to evaluate the effects of different types of alum on soluble metals in poultry litter. The objective of this study was conducted to investigate changes in soluble metal from poultry litter with different types of aluminum sulfate (alum) under laboratory condition. The treatments used in this study, which were mixed in the upper 1 cm of litter or sprayed onto the litter surface, were 4 g alum, 8 g alum, 8.66 g liquid alum, 17.3 g liquid alum, 11.2 g A7 (high acid alum), and 22.4 g A7 (high acid alum)/100 g litter. Applying different types of alum to poultry litter reduced (P<0.05) concentrations of soluble Fe (9 to 54%), Cu (9 to 49%) and Zn (11 to 40%), relative to untreated litter, whereas it increased Ca and Mg (P<0.05). Mean soluble Fe and Cu levels in poultry litter from different types of alum decreased in the order: 22.4 g A7 (54% and 49%) > 17.3 g liquid alum (48% and 42%) > 8 g alum (48% and 31%) > 4 g alum (28% and 10%) > 8.6 g liquid alum (10% and 9%) > 11.2 g A7 (8.6% and 9%). Additionally, the high reduction in soluble Zn concentration was 4 g alum (40%), followed by 8 g alum (26%), 22.4 g A7 (25%), 17.3 g liquid alum (23%), 8.66 g liquid alum (18%), and 11.2 g A7 (11%), respectively. In conclusion, the current studies suggest that treating poultry litter with different types of alum can be applied to reduce soluble metal (Fe, Cu, and Zn) and to develop a production to merchandise for poultry litter that would result in reduction in pollutants from these materials. Furthermore, in order to improve environmental management in the poultry industry, the use of alum, liquid alum and high acid alum all should be provided a valid means of reducing negative environmental impact.

The effect of calcium metaphosphate bone graft materials on bone regeneration (생분해성 Calcium Metaphosphate골이식재의 골조직재생효과에 관한 연구)

  • Chae, Han-seung;Lee, Yong-Moo;Yang, Seung-Min;Chun, Sung-Soo;Kim, Suk-Young;Ku, Young;Choung, Chong-Pyoung;Han, Soo-Boo;Choi, Sang-Mook;Rhyu, In-Chul
    • Journal of Periodontal and Implant Science
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    • v.33 no.1
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    • pp.13-26
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    • 2003
  • Periodontal regeneration therapy with bone-substituting materials has gained favorable clinical efficacy by enhancing osseous regeneration in periodontal bony defect. As bone-substituting materials, bone powder, calcium phosphate ceramic, modified forms of hydroxyapatite, and hard tissue replacement polymer have demonstrated their periodontal bony regenerative potency. Bone-substituting materials should fulfill several requirements such as biocompatibility, osteogenecity, malleability, biodegradability. The purpose of this study was to investigate biocompatibility, osteo-conduction capacity and biodegradability of $Na_2O$, $K_2O$ added calcium metaphosphate(CMP). Beta CMP was obtained by thermal treatment of anhydrous $Ca_2(H_2PO_4)_2$. $Na_2O$ and $K_2O$ were added to CMP. The change of weight of pure CMP, $Na_2O$-CMP, and $K_2O$-CMP in Tris-buffer solution and simulated body fluid for 30 days was measured. Twenty four Newzealand white rabbits were used in negative control, positive control(Bio-Oss), pure CMP group, 5% $Na_2$-CMP group, 10% $Na_2O$-CMP goup, and 5% $K_2O$-CMP group. In each group, graft materials were placed in right and left parietal bone defects(diameter 10mm) of rabbit. The animals were sacrificed at 3 months and 6 months after implantation of the graft materials. Degree of biodegradability of $K_2O$ or $Na_2O$ added CMP was greater than that of pure CMP in experimental condition. All experimental sites were healed with no clinical evidence of inflammatory response to all CMP implants. Histologic observations revealed that all CMP grafts were very biocompatible and osseous conductive, and that in $K_2O$-CMP or $Na_2O$-CMP implanted sites, there was biodegradable pattern, and that in site of new bone formation, there was no significant difference between all CMP group and DPBB(Bio-Oss) group. From this result, it was suggested that all experimental CMP group graft materials were able to use as an available bone substitution.