• Journal of Ginseng Research
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• v.32 no.2
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• pp.155-160
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• 2008

The North American ginseng (Panax quinquefolius L.) seed crop varies from year to year. The ability to hold stratified seed for a year would ensure continuity of seed supply and no interruption in production cycles. Seed drying and rehydration protocols at room temperature $(21{\pm}2^{\circ}C)$ were developed. These protocols and seed storage at 4 ${\pm}1^{\circ}C$ and 35%, or variable, relative humidity (RH) allowed the holding of stratified seed for one year and then establishment of the following five treatments in field plots: Trt.1 : dried 2005 stratified seed (seed harvested Fall 2004) held at $4^{\circ}C$ and at variable humidity; Trt.2 : 2006 stratified seed planted directly into the field; Trt.3 : 2005 stratified seed dried in October 2005 and held at $4^{\circ}C$ and 35% RH ; Trt.4 : 2005 stratified seed held in moist sand from October to December 2005 at room temperature $(21{\pm}2^{\circ}C)$ and then in December dried and held at $4^{\circ}C$ and 35 % RH; Trt.5 : 2005 stratified seed held in moist sand from October to December 2005 at room temperature and then in December dried and held at $-12^{\circ}C$ Seedling emergence was best in Trts. 2 and 4 with 67.3 and 65.1% respectively which is similar to the industry expected rate of 68% after regular stratification. Seedling growth was similar in Trts. 2 and 4 with root dry weights of 172 and 159 mg respectively in mid-August. Therefore, if holding stratified seed in August/September for one year is desired, the seed can be placed in moist sand until December and then dried and stored at $4^{\circ}C$ and 35% RH. These seed can be planted in the following August/September and will germinate and grow in the following year to give an acceptable crop.

• Journal of the Korean Chemical Society
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• v.28 no.5
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• pp.315-322
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• 1984

The dependence of polarographic parameters on the pressure for the reduction of In(III), Cr(III), Cd(II), Pb(II), Mn(II), Co(II), Tl(I) in 0.1M KCl aqueous solution at the dropping mercury electrode have been discussed. In this experiment the temperature varied from $25^{\circ}C\;to\;35^{\circ}C$ and the pressure ranges from 1 atmosphere to 1,800 atmospheres. By increasing the pressure the reduction half-wave potentials of all metal ions are shifted markedly to more negative values and the diffusion currents of all metal ions become considerably larger. The slope of the linear relationship of E vs. log[$\frac{id-i)}{i}$] become much larger with increase in pressure, which indicates more irreversible reduction. The temperature coefficient observed over the range of the temperature from $25^{\circ}C\;to\;35^{\circ}C$ are not sensitive with increase in pressure.

• International Journal of Industrial Entomology and Biomaterials
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• v.15 no.1
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• pp.69-73
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• 2007

To establish the indoor-rearing system of the emma field cricket, Teleogryllus emma, the effects of temperature on development and reproduction of the emma cricket have been studied. The influence of temperature on developmental periods of emma field cricket was investigated under the eight temperatures of 15, 18, 21, 25, 27, 29, 31 and $35^{\circ}C$, under $60{\pm}5%$ R.H. and 16L:8D photoperiod. The developmental periods of T. emma nymphs had a range of 124.8 days to 44.4 days at the temperature of $21^{\circ}C$ and $35^{\circ}C$, respectively. At $15^{\circ}C$ and $18^{\circ}C$, however, all tested individuals died before emergence. The highest survival rate was 90% at $25^{\circ}C$, but there were no statistically significant differences among the temperatures. The adult weight increased with increasing temperatures although the weight at $35^{\circ}C$ was decreased. In addition, the influence of temperature on reproduction of emma field cricket was investigated under three temperatures $22^{\circ}C,\;25^{\circ}C\;and\;28^{\circ}C$, under $60{\pm}5%$ R.H. and 16L:8D photoperiod. The longevity of female/male adults were 65.8/79.2 days, 68.5/67.8 days, 46.8/57.4 days at the temperature $22^{\circ}C,\;25^{\circ}C\;and\;28^{\circ}C$, respectively. The preoviposition periods were 32.5 days at $22^{\circ}C,\;22.9^{\circ}C$ days at $25^{\circ}C$ and 22.1 days at $28^{\circ}C$. The highest average fecundity per female was 737.3 at $25^{\circ}C$.

• Microbiology and Biotechnology Letters
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• v.20 no.2
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• pp.122-128
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• 1992

Runaway replication plasmid pSY35AT was used for the production of $cI_{857}$ repressor protein. E, coli MC1065 having plasmid pSY35AT was shifted from $30^{\circ}C$ to $37^{\circ}C$ $cI_{857}$ repressor protein produced was purified by a modification of the purification method of wild type cI repressor. The concentration of purified $cI_{857}$ repressor protein was 0.11 mg/ml. The binding assay of $cI_{857}$ repressor and right promoter - right operater ($P_RO_R$) labeled with $^3H-CTP$ was done. Relative activity of purified cIx5, repressor was higher about 23 times than that of cell free extract. A higher value of the temperature in the binding assay led to a lower value of the binding activity.

• Journal of Embryo Transfer
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• v.16 no.2
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• pp.133-138
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• 2001

The aim of this study was to identify the method on extended canine semen exposed to freezing as assessed by motility, survival and acrosomal changes following different freezing ramp rates. Five ejaculates collected by digital manipulation twice weekly from three dogs (Shih-Tzu) were added Tris-Egg Yolk (TE) buffer and divided into 4 aliquots according to formulation of our laboratory. After cooling to 4$^{\circ}C$ by ramp rate of 0.6$^{\circ}C$/min, the samples frozen by ramp rates of 1.6$^{\circ}C$/min to -$25^{\circ}C$, 3$^{\circ}C$/min to -35$^{\circ}C$, 8.9$^{\circ}C$/min to -7$0^{\circ}C$ and 19$^{\circ}C$/min to -11$0^{\circ}C$, respectively, and then stored in L$N_2$for 2days. Each sample was evaluated on their motility, survivability and acrosome integrity at different thawing temperature. The ramp rate of 3$^{\circ}C$/min to -35$^{\circ}C$/h for freezing and thawing temperature of 37$^{\circ}C$ obtained the highest results to improve survivability, motile spermatozoa and intact acrosome appearance than other onditions. In conclusion, we may suggest freezing semen for canine artificial insemination is more efficient with freezing at a ramp rate of -3$^{\circ}C$/min to -35$^{\circ}C$ and thawing with a water bath adjusted to 37$^{\circ}C$.

• Parasites, Hosts and Diseases
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• v.50 no.3
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• pp.239-242
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• 2012

The influence of temperature on the development and embryonation of Ascaris suum eggs was studied using coarse sand medium in an environmental chamber with 50% humidity. The time required for development and embryonation of eggs was examined under 3 different temperature conditions, $5^{\circ}C$, $25^{\circ}C$, and $35^{\circ}C$. A. suum eggs did not develop over 1 month at the temperature of $5^{\circ}C$. However, other temperature conditions, $25^{\circ}C$ and $35^{\circ}C$, induced egg development to the 8-cell-stage at days 5-6 after incubation. All eggs examined developed to the 8-cell stage at day 6 after incubation in the sand medium at $25^{\circ}C$. The higher temperature, $35^{\circ}C$, slightly accelerated the A. suum egg development compared to $25^{\circ}C$, and the development to the 8-cell stage occurred within day 5 after incubation. The formation of larvae in A. suum eggs at temperatures of $35^{\circ}C$ and $25^{\circ}C$ appeared at days 17 and 19 after incubation, respectively. These findings show that $35^{\circ}C$ condition shortens the time for the development of A. suum eggs to the 8-cell-stage in comparison to $25^{\circ}C$, and suggest the possibility of accelerated transmission of this parasite, resulting from global warming and ecosystem changes.

• Korean Journal of Environmental Agriculture
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• v.29 no.4
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• pp.348-353
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• 2010

The current study investigated the fate of organic matter in piggery slurry under two different store systems(closed store system and open store system) in association with different temperature. Thirty days after storing in both systems at $20^{\circ}C$, it was observed that the content of organic matter remained in piggery slurry with closed store system was twice more than that with open store system implying more efficient degradation of organic matter with open store system. Temperature also influenced on the organic matter degradation in piggery slurry as shown decline in TS and VS contents as the temperature increased. With store at $35^{\circ}C$, 29% of initial organic matter was reduced while there was only 23% reduction of organic matter at $20^{\circ}C$. There was no difference in the type of organic fatty acids(VFAs) produced under the range of temperature(20, $35^{\circ}C$) simulating summer condition. Increases in organic fatty acids contents with hydrolysis and acid producing microbial was observed from 15 days after initiating store of the piggery slurry and the total organic acid amount produced 30 days after store was $2,829\;mg{\cdot}COD/L$ and $9,123\;mg{\cdot}COD/L$ at $20^{\circ}C$ and $35^{\circ}C$, respectively. These corresponded to 5.4% and 17.4% of the initial organic matter contents in piggery slurry, respectively.

• Journal of the Korean Chemical Society
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• v.26 no.4
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• pp.205-209
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• 1982

Anisotropic rotation of $CDCl_3$ in neat liquid is investigated by the analysis of ${\nu}_1$ band of Raman spectrum and the diffusion constant($D_{\perp}$) for the tumbling motion is obtained. The diffusion constant ($D_{II}$) for the spinning motion is obtained from the above $D_{\perp}$ value and the chlorine-35 nuclear quadrupole relaxation time. The diffusion constants thus obtained seem to agree very well with the ones obtained from $^2H$ and $^35C$l in NQR results within experimental errors. The data suggest fairly anisotropic character of reorientational motions in neat $CDCl_3$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.1
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• pp.7-12
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• 1994

The kinetics of browning reaction of dried shrimp powder samples were investigated during storage. The temperature conditions of the ding process were $25^{\circ}C\;and\;45^{\circ}C$, and the samples were stored at water activity ($a_w$) of 0.33, 0.44, 0.52, 0.65 and temperatures of $35^{\circ}C,\;45^{\circ}C,\;55^{\circ}C$ and temperature fluctuations between $33^{\circ}C\;and\;55^{\circ}C$. When the shrimp was dried at $25^{\circ}C$ the activation energies obtained from the Arrhenius plot ranged from 13.57 to 14.33 kcal/mol. From these energies of activation, the $Q_{10}$ values at $25^{\circ}C$ showed 1.93 to 2.00. In the case of drying at $45^{\circ}C$ the activation energies were $13.12{\sim}13.61\;kcal/mol$ and $Q_{10}$ values were $1.89{\sim}1.93$, respectively. In addition, a storage study under square-wave fluctuating temperature conditions was carried out by varying the shrimp sample temperature between $35^{\circ}C\;and\;55^{\circ}C$ within 7 days regular fluctuation cycle. The data obtained from the fluctuating temperature storage study will be used in the prediction of shelf-lives. The shelf-lives assessed at $25^{\circ}C$ from the accelerated shelf-life tests ranged from 4 days at aw 0.65 to 139 days at aw 0.33.

• KSBB Journal
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• v.18 no.4
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• pp.329-334
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• 2003

The present study was aimed at investigating the regulatory mechanism in tissue-specific expression of insulin-like growth factor-I (IGF-I) gene. The expression of IGF-I gene was determined by a solution hybridization/RNase protection assay using total RNA prepared from rat liver or brain of various ages. The levels of IGF-I transcripts were increased in liver gradually after birth, but decreased in brain. By using an oligonucleotide (FRE) corresponding to the C/EBP binding site of the rat IGF-I exon 1, multiple forms of C/EBP${\alpha}$ and C/EBP${\beta}$ proteins, which have DNA-binding activity, were detected in the rat liver or brain. Western immunoblot and southwestern analyses show that p42$\^$C/EBP${\alpha}$/, p38$\^$C/EBP${\alpha}$/, p35$\^$C/EBP${\alpha}$/, p38$\^$C/EBP${\beta}$/, and p35$\^$C/EBP${\beta}$ form specific complexes with the IGF-I exon 1 oligonucleotide in liver nuclear extract and that p42$\^$C/EBP${\alpha}$/ and p38$\^$C/EBP${\beta}$/ form complexes in brain. These data suggest that the formation of FRE-C/EBP isoform complexes may play important roles in the tissue-specific regulation of IGF-I gene expression.