• 대한수의학회지
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• 제40권1호
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• pp.86-93
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• 2000

Paratuberculosis (Johne's disease), a chronic enteritis produced by Mycobacterium paratuberculosis, affects a large proportion of ruminants in all continents and causes important economic losses. The identification of well-characterized and species-specific components of M paratuberculosis would provide the means to improve the specificity and sensitivity of immunodiagnostic assays for Johne's disease. The aims of this study were to express the recombinant C-terminal of 34kDa protein (rC34P) of M paratuberculosis in E coli and to investigate the effectiveness of this protein in detecting antibodies to the native protein in sera from paratuberculosis infected cattle. The C-terminal of the gene encoding the 34kDa protein was amplified by polymerase chain reaction from the chromosomal DNA of M paratuberculosis (ATCC 19698) and cloned into vector pGEX-4T-2. Then, cloned plasmid was transformed into E coli DH5${\alpha}$ and the rC34P was overexpressed. The rC34P was purified by affinity chromatography and gel filtration. The rC34P was examined antigenicity by Western blot. The rC34P was reactive with culture positive bovine serum and hyperimmune rabbit anti-M paratuberculosis serum but was not reactive with culture negative bovine serum and tuberculin positive bovine serum in Western blot. In conclusion, the rC34P produced in this study is expected as a useful candidate for antigen in serological diagnosis of Johne's disease.

• 대한수학회논문집
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• 제33권4호
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• pp.1141-1158
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• 2018

Hahn difference operator $D_{q,{\omega}}$ which is defined by $$D_{q,{\omega}}g(t)=\{{\frac{g(gt+{\omega})-g(t)}{t(g-1)+{\omega}}},{\hfill{20}}\text{if }t{\neq}{\theta}:={\frac{\omega}{1-q}},\\g^{\prime}({\theta}),{\hfill{83}}\text{if }t={\theta}$$ received a lot of interest from many researchers due to its applications in constructing families of orthogonal polynomials and in some approximation problems. In this paper, we investigate sufficient conditions for stability of the abstract linear Hahn difference equations of the form $$D_{q,{\omega}}x(t)=A(t)x(t)+f(t),\;t{\in}I$$, and $$D^2{q,{\omega}}x(t)+A(t)D_{q,{\omega}}x(t)+R(t)x(t)=f(t),\;t{\in}I$$, where $A,R:I{\rightarrow}{\mathbb{X}}$, and $f:I{\rightarrow}{\mathbb{X}}$. Here ${\mathbb{X}}$ is a Banach algebra with a unit element e and I is an interval of ${\mathbb{R}}$ containing ${\theta}$.

• 한국분말야금학회:학술대회논문집
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• 한국분말야금학회 2006년도 Extended Abstracts of 2006 POWDER METALLURGY World Congress Part2
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• pp.1165-1166
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• 2006

An experiment was carried out to investigate the effect of Ba Stearate as a reducing agent on the magnetic and physical properties of anisotropic $BaFe_2-W$ type ferrite magnets. It was found that the magnetic properties of $BaO{\cdot}8.5Fe_2O_3$ were improved by adding 0.3 wt% of Ba Stearate, 0.5 wt% of $SiO_2$, and 0.5 wt% of CaO together. The optimum conditions for making magnets were as follows; semisintering condition: $1350^{\circ}C{\times}4.0$ h in nitrogen gas atmosphere, drying condition: $180^{\circ}C{\times}2.0$ h in air, sintering condition: $1160^{\circ}C{\times}1.5$ h in nitrogen gas atmosphere. Magnetic and physical properties of a typical sample were $J_m$ = 0.46 T, $J_r$ = 0.43 T, $H_{cJ}$ = 182.3 kA/m, $H_{cB}$ = 177.2 kA/m, $(BH)_{max}$ = 33.8 kJ/$m^3$, $T_C$ = $495^{\circ}C$ and $K_A$ = $2.65{\times}10^5\;J/m^3$ and $H_A$ = 1332 kA/m.

• 한국균학회지
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• 제22권3호
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• pp.254-259
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• 1994

P. chrysosporium BKM-F-1767 및 C. subvermispora FP90031-SP. 균사를 이용하여 ligninase와 laccase 활성제고를 위한 배앙방법을 검토한 결과는 다음과 같다. P. chrysosporium의 왕복진탕배양시 (150 rpm) 배양액량은 45-60 ml가 ligninase 활성제고에 적합하였으며 60 ml 배양은 최대활성 출현일이 빨리 나타났다. C. subvermispora의 정치배양시, 2% M.E.+0.1% Y.E.+0.1% T.W.20+1mMVA 처리에서 ligninase 활성이 가장 높았고 laccase의 활성은 2% M.E.+0.1% Y.E.+0.1% T.W.20+6 mMBA에서 최고 수준을 나타내었다.

• 대한수의학회지
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• 제39권6호
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• pp.1073-1080
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• 1999

Previous studies suggested that the inhibition of thyroxine ($T_4$) release by ${\alpha}_1$-adrenoceptor and muscarinic receptor stimulation results in activated protein kinase C (PKC) from mouse and guinea pig thyroids. In the present study, the effect of carbachol, methoxamine, phorbol myristate acetate (PMA), and R59022 on the release of $T_4$ from the mouse, rat, and guinea pig thyroids was compared to clarify the role of PKC in the regulation of the release of $T_4$. The thyroids were incubated in the medium containing the test agents, samples of the medium were assayed for $T_4$ by EIA kits. Forskolin, an adenylate cyclase activator, chlorophenylthio-cAMP sodium, a membrane permeable analog of cAMP, and isobutyl-methylxanthine, a phosphodiesterase inhibitor, like TSH (thyroid stimulating hormone), enhaced the release of $T_4$ from the mouse, rat, and guinea pig thyroids. Methoxamine, an ${\alpha}_1$-adrenoceptor agonist, inhibited the TSH-stimulated release of $T_4$ in mouse, but not rat and guinea pig thyroids. In contrast, carbachol, a muscarinic receptor agonist, inhibited the release of $T_4$ in guinea pig, but not mouse and rat thyroids. These inhibition were reversed by prazosin, an ${\alpha}_1$-adrenoceptor antagonist or atropine, a muscarinic antagonist or $M_1$- and $M_3$-muscarinic antagonists, in mouse or guinea pig thyroids. In addition, staurosporine, a PKC inhibitor, reversed methoxamine or carbachol inhibition of TSH stimulation. Furthermore, PMA, a PKC activator, and R59022, a diacylglycerol (DAG) kinase inhibitor, inhibited the TSH-stimulated release of $T_4$ in mouse, rat, and guinea pig thyroids. These inhibition were blocked by staurosporine. These findings suggest that the activation of receptor or DAG inhibits TSH-stimulated $T_4$ release through a PKC-dependent mechanism in thyroid gland.

• 대한치과보철학회지
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• 제27권1호
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• pp.55-81
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• 1989

Recently the instantaneous center concept has been to understand the biomechanics by which a tissue derangement causes a mechanical derangement in human joint. Therefore, to understand the biomechanics of temporomandibular joint (T.M.J.) as a part of human joint, it is necessary to clarify the instantaneous center of rotation (I.C.R.) in the mandibular movement. Twenty male subjects without T.M.J. disorder and mandibular deviation during the mandibular movement were selected for this study. The habitual opening and closing paths were recorded on the paper of the sagittal metal plate by two pencil markers connected to the resin open clutch attached on the lower teeth, which was designed for this study. The coordinates of the 33-target points and the 109-anatomical landmarks were obtained using a Summagraphic digitizer connected to a 18AT computer. The original raw data of the opening and closing paths were smoothed by B-spline curve fitting technique and then the I.C.R. pathways were determined mathematically by the computer using algorithm for finding the I.C.R. of a planer rigid body model. Also the opening and closing movements of the mandible were simulated according to the determined I.C.R. The results obtained from this study were as follows. 1. At the early opening and the last closing, I.C.R's were almost distributed around the mastoid process outside the mandibular body without the presence in the region of the mandibular condyle. 2. The I.C.R. pathway showed variable patterns to each subject at the opening and closing movements. 3. The K constant with uniform pattern was obtained by the rotation angle times the radius, which was assumed to the index of the mandibular movement. 4. The opening and closing movements of the mandible were simulated by the I.C.R. pathways at the habitual opening and closing movements. 5. The mandibular condyle was rotated or translated accordng to the relative rotation angle and radius of the determinant factors of K contant.

• 충청수학회지
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• 제22권3호
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• pp.579-586
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• 2009

Let $\varphi$ be a complete probability measure on $\mathbb{R}$, let $m_{\varphi}$ be the analogue of Wiener measure over paths on [0, T] and let f(t) be continuously differentiable on [0, T]. In this note, we give the analogue of Wiener measure $m_{\varphi}$ of {x in C[0, T]$\mid$x(0) < f(0) and $x(s_0){\geq}f(s_{0})$ for some $s_{0}$ in [0, T]} by use of integral equation techniques. This result is a generalization of Park and Paranjape's 1974 result[1].

• 설비공학논문집
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• 제26권3호
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• pp.115-121
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• 2014

HVAC&R system costs can often be one of the most expensive components, representing approximately 15% of the total construction cost for office buildings. Despite their significant importance, there is a lack of a consistent and homogeneous framework to approximate the estimate research. This research deals with the prediction methodology of HVAC&R system cost with the aim of establishing a common idea for the analysis of the construction cost estimate. Our approach deals with the concept of an HVAC&R set that is composed of subsystems. The matrix combination analysis is examined, and total 960 HVAC&R system cost estimation can be implemented to large scale office buildings.

• 한국진공학회:학술대회논문집
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• 한국진공학회 1998년도 제14회 학술발표회 논문개요집
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• pp.18-18
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• 1998

F First I will introduce our works how to improve the crystal growth t technique for Rare earth pnictides and chalcogenides. All these substances h have hi양1 vapor pressure and high melting print up to 3$\alpha$)()C. 까len we employ m the tungsten or molybden crucibles and enclose the sample by 빼e welding of m the lid with high current electron beam. We cannot elevate the temperature up t to 3$\alpha$)()C without suitable radiation shield because rate of radiation loss r rapidly increase in such a high temperature regions. There were no good r radiation shield but we discovered that the p-BN could work as an excellent r radiation shield after checking of the many substances. S Secondly I will show several interesting and unusual 뻐ysical properties of obtained crystals under high magnetic field, hi양1 pressure and also i including angle resolve photoemission spectroscopy. I will stress the p properties of the low carrier concentration with strong correlation on C댐, U USb and Yb4As3

• 한국세라믹학회지
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• 제49권3호
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• pp.205-215
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• 2012

It has been studied the crystal and block structures of the hexagonal ferrites with M, W, Y and Z types prepared by various coprecipitation-oxidation method. The structures have been refined with a Rietveld analysis of the powder X-ray diffraction pattern with high precision ($R_{WP}$ <0.09, $R_I$ <0.03). The density difference between the S-blocks was proportioned to the cobalt contents in hexagonal ferrites, but that between the R or T-blocks was relatively small. Compared with the blocks and cation-oxygen polyhedra in BaM ($BaFe_{12}O_{19}$), those were bulky to the normal direction for the c-axis in $Co_2W$ ($BaCo_2Fe_{16}O_{27}$) and to the parallel direction for the c-axis in $Co_2Y$ ($Ba_2Co_2Fe_{12}O_{22}$) and $Co_2Z$ ($Ba_3Co_2Fe_{24}O_{41}$). The S-blocks of $Co_2W$, $Co_2Y$, and $Co_2Z$ were unstable and distorted. Because the T-block of $Co_2Z$ was unstable, the T-block was decomposed into the Ba-rich phase and $Co_2W$ at high temperatures above $1200^{\circ}C$. A standard powder X-ray diffraction pattern for $Co_2Z$ was proposed as well.