• Title/Summary/Keyword: C-line

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Growth Inhibition of Red Ginseng Extracts Against Human Tumor Cell Line by Clonogenic Assay (Clonogenic assay을 이용한 홍삼추출물의 인체종양세포에 대한 증식억제효과)

  • Kim, Chang-Han;Lee, Gyeong-Ho;Byeon, Eun-Gyeong
    • Journal of Ginseng Research
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    • v.22 no.3
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    • pp.188-192
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    • 1998
  • We established the model of clonogenic assay with human tumor cell line such as Calu-3 (lung carcinoma), HEC- lB (endometrial adenocarcinoma) , HEp-2 (larnyx carcinoma), Hs-5787 (breast carcinoma), K-562 (chronic myelogenous leukemia), SF-188 (brain carcinoma), SNU-1 (stomach carcinoma) and WiDr (colon carcinoma) . We investigated growth inhibition of solvent (EtOH, MeOH) and water (100$^{\circ}C$, 121$^{\circ}C$) extracts from Korean red ginseng by clonogenic assay. The results of clonogenic assay showed that EtOH extract had growth inhibition against Calu-3, SF-188 and SNU-1, MeOH extract had growth inhibition against Calu-3, Hs-5787, K-562, and WiDr, but water extract at 100$^{\circ}C$ and water extract at 121$^{\circ}C$ had not growth inhibition against used cell lines.

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C IV Emission-line Detection of the Supernova Remnant RCW 114

  • Kim, Il-Joong;Min, Kyoung-Wook;Seon, Kwang-Il;Han, Won-Young;Edelstein, Jerry
    • Bulletin of the Korean Space Science Society
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    • 2009.10a
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    • pp.33.3-33.3
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    • 2009
  • We report the detection of the C IV $\lambda\lambda1548$, 1551 emission line in the region of the RCW 114 nebula using the FIMS/SPEAR data. The observed C IV line intensity indicates that RCW 114 is much closer to us than HD 156385, a Wolf-Rayet star that was thought to be associated with RCW 114 in some of the previous studies. We also found the existence of a small H I bubble centered on HD 156385, with a different LSR velocity range from that of the large H I bubble which was identified previously as related to RCW 114. These findings imply that the RCW 114 nebula is an old supernova remnant which is not associated with the Wolf-Rayet star, HD 156385. Additionally, the global morphology of the C IV and $H{\alpha}$ emissions shows that RCW 114 has evolved in a non-uniform interstellar medium.

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A Novel Air-Bridge Type Gate-Data Line Inter-Crossing to Reduce Signal Delay for Large Size AMLCD (대면적 AMLCD의 신호 지연 감소를 위해 Air-gap을 갖는 게이트-데이터 라인 교차 구조)

  • Park, Jin-Woo;Kang, Ji-Hoon;Lee, Min-Cheol;Han, Min-Koo
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.48 no.12
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    • pp.768-772
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    • 1999
  • A new TFT-LCD panel with air-bridge type gate to data line inter-crossing has been proposed and its characteristics have been measured. The proposed structure has air-gap between gate and data line inter-crossing. This air-bridge TFT-LCD panel has very small capacitance between gate and data line. The new panes structure achieves 9 times fast signal propagation compared with conventional panel, which enables to have enough design margin for 20-inch diagonal and larger size UXGA panel. We have examined thermal and mechanical durability of new panel to verify applicability for commercial AMLCD production. After TEOS and polyimide passivation, this panel withstood a thermal stress at $250^{\circ}C$ and a mechanical stress during the rubbing process.

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Antioxidant activity analysis of Catechin compounds in Korean green tea using HPLC On-line $ABTS^{+}$ Antioxidant screening system (HPLC On-line $ABTS^{+}$ Antioxidant screening 시스템을 이용한 한국산 녹차로부터 Catechin compounds의 황산화 활성분석)

  • Lee, Kwang-Jin
    • KSBB Journal
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    • v.23 no.1
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    • pp.96-100
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    • 2008
  • In this work, we describes analysis of the antioxidant potential of Korean green tea phenolics using an high-performance liquid chromatography (HPLC) on-line $ABTS^{+}$ antioxidant screening method. In conjunction with the analysis of their 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) radical scavenging ability, the extraction of catechine compounds from Korean green tea were performed by various temperature and time. The optimum operating conditions were experimentally determined to analyze the catechine compounds in the pretreatment extracts. From the results, the extraction temperature $60^{\circ}C$, time 3 min was selected as an optimal antioxidant activity condition. The analysis by $C_{18}$ column was performed, the flow rate of mobile phase and UV wavelength was fixed at 1.0 ml/min and 254 nm, respectively. the mobile phase was composed from acetonitrile and water, and the gradient elution mode were applied.

Learning Algorithm of Dynamic Threshold in Line Utilization based SARIMA model (SARIMA 모델을 기반으로 한 선로 이용률의 동적 임계값 학습 기법)

  • Cho, Kagn-Hong;Ahn, Seong-Jin;Chung, Jin-Wook
    • The KIPS Transactions:PartC
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    • v.9C no.6
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    • pp.841-846
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    • 2002
  • We applies a seasonal ARIMA model to the timely forecasting in a line utilization and its confidence interval on the base of the past data of the line utilization that QoS of the network is greatly influenced by. And this paper proposes the learning algorithm of dynamic threshold in line utilization using the SARIMA model. We can find the proper dynamic threshold in timely line utilization on the various network environments and provide the confidence based on probability. Also, we have evaluated the validity of the proposed model and estimated the value of a proper threshold on real network. Network manager can overcome a shortcoming of original threshold method and maximize the performance of this algorithm.

Studies on toxicity of ochratoxin A to chromosomes of turmor cell-line (종양세포(腫瘍細胞)의 염색체(染色體)에 대한 오크라톡신 A의 독성(毒性)에 관한 연구(硏究))

  • Yoon, Wha-jung;Roh, Min-hee;Kim, Kang-ryun
    • Korean Journal of Veterinary Research
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    • v.29 no.2
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    • pp.51-57
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    • 1989
  • This study was performed to investigate the toxicity of ochratoxin A (OA) to the chromosomes of $K_{562}$ tumor cell-line in vitro. The results of this experiment were as follows: 1) Chromosomes of $K_{562}$tumor cell-line resulted in pseudotriploidy on the control group. Chromosomes of $K_{562}$ tumor cell-line treated with OA resulted in heteroploidy compared with the control group. The mean number of chromosomes in the karyotype of the control group (60) were 7 in the A group, 5 in the B group, 20 in the C+X group, 7 in the D group, 9 in the E group, 6 in the F group, and 6 in the G+Y group respectively. The number of chromosomes were increased as follows: Treating with $0.7{\mu}M$ OA, the number of chromosomes were increased one in E and F group, two in G+Y group compared with control group. In treated with $1.5{\mu}M$ OA, the increasing number of chromosome was one in E and F group. In treated with $3{\mu}M$ OA, E and F group was increased one and G+Y group were increased two chromosomes compared with control group. But in treated with $6{\mu}M$ OA, the number of chromosome in G+Y group was decreased one. 2) $K_{562}$ tumor cell line treated with OA showed Philadelphia-Chromosome in the long arm of the G group karyotype chromosome. The rate of chromosome aberration in $K_{562}$ tumor cell-line treated with OA was 77% in $0.7{\mu}M$ OA group, 71% in $1.5{\mu}M$ OA group, 82% in $3{\mu}M$ OA group and 94% in $6{\mu}M$ OA group respectively. The rate of chromosome aberration of $K_{562}$ tumor cell-line treated with OA was high in the high dose level of OA, and chromosome aberration of $K_{562}$ tumor cell-line treated with OA showed deletion, minute, dicentric-chromosome and translocation in the long arm of the C-group karyotype. As a result of this study, the toxicity of OA showed deletion, minute, dicentric-chromosome and translocation in the long arm of the C-group karyotype, and then, the toxicity of OA resulted in the damage to RNA and protein synthesis in $K_{562}$ tumor cell-line, and the C-group karyotype of $K_{562}$ tumor cell-line was target of the toxicity of OA.

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A Novel Method to Measure Superior Migration of the Humeral Head: Step-off of the C-line

  • Park, Kyoung Jin;Eun, Hyeon Jun;Kim, Yong Min;Yoo, Jun Il;Lim, Chae Ouk
    • Clinics in Shoulder and Elbow
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    • v.19 no.3
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    • pp.125-129
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    • 2016
  • Background: Superior migration of humeral head has been conventionally determined by measuring the acromiohumeral distance (AHD), We sought to devise a novel measurement system more reliably and accurately than AHD. We described a structural landmark called 'C-line'. In this study, we investigated the clinical usefulness of 'step-off of the C-line (SOC)' compared to that of AHD. Methods: The C-line formed from the medial margin of the proximal humeral head continuing up to the inferior margin of the articular glenoid and then to the lateral border of the scapula. The superior migration of the humeral head triggered by a rotator cuff tear introduces a discontinuity in this C-line. We measured the distance of this discontinuity. We enrolled 144 patients who underwent a rotator cuff repair. We selected 58 controls who didn't have any cuff lesions apparent on magnetic resonance imaging. Using radiographs derived from standardized true anteroposterior views of the shoulder, we measured the SOC and the AHD. We used t-tests for statistical analyses. Results: A rotator cuff tear was associated with an increase in SOC and a decrease in AHD. In control group, the mean SOC was $1.29{\pm}1.71mm$ and AHD was $9.71{\pm}2.65mm$. In cuff tear group, the mean SOC was $3.15{\pm}3.41mm$ and AHD was $8.28{\pm}1.76mm$. The mean SOCs of the patient group in relation to the mean SOC of the control group according to tear size, the SOCs of medium tear and lager groups showed statistically significant increase (p<0.05). Conclusions: The SOC may be a similarly effective to diagnose cuff tears of medium size and larger compared with AHD.

The Apple Rootstock Transgenic M.26 (Malus pumila) with Enhanced Rooting Ability (발근력이 향상된 사과 대목 M.26 형질전환체)

  • Kim, Jeong-Hee;Kwon, Soon-Il;Shin, Il Sheob;Cho, Kang-Hee;Heo, Seong;Kim, Hyun Ran
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.482-487
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    • 2009
  • The apple rootstock M.26 (Malus pumila) is very popular apple rootstock with semi-dwarf habit and the trees on M.26 produce high quality fruit at a young age. Since it is prone to poor prop ability to soil, however, young trees require staking in windy locations. The rolC gene was introduced into M.26 by Agrobacterium tumefaciens LBA4404 harboring pBI121 to obtain its transformants with dwarfism and enhanced rooting ability. One regenerated transgenic line was confirmed by polymerase chain reaction (PCR) analysis and Southern blot analysis of genomic DNA for the existence of rolC gene. The characteristics of transgenic line in vitro were not significantly different from non-transgenic line except for the active root formation and lateral root number. The rolC transgenic line showed reduced stem length and increased root number in vitro. Rooting ability was examined in the isolated greenhouse after mound layering. Compared to non-transgenic M.26, rolC transgenic line showed significantly higher rooting ability. The transgenic line did not show any other observable variation in shoot phenotype compared with non-transgenic line excepting increased branching