• Asian-Australasian Journal of Animal Sciences
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• v.26 no.3
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• pp.309-315
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• 2013

MicroRNAs are a class of endogenous small RNAs that play important roles in post-transcriptional gene regulation by directing degradation of mRNAs or facilitating repression of target gene translation. In this study, three small RNA cDNA libraries from the mammary gland tissues of Laoshan dairy goats (Capra hircus) were constructed and sequenced, individually. Through Solexa high-throughput sequencing and bioinformatics analysis, we obtained 50 presumptive novel miRNAs candidates, and 55,448 putative target genes were predicted. GO annotations and KEGG pathway analyses showed the majority of target genes were involved in various biological processes and metabolic pathways. Our results discovered more information about the regulation network between miRNAs and mRNAs and paved a foundation for the molecular genetics of mammary gland development in goats.

• Journal of The Korean Astronomical Society
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• v.11 no.1
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• pp.1-30
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• 1978

The four dimensional classification of globular dusters with the parameters, Z, Y, age and HB type is presented defining two new parameters. $(B-V)_{1/2}\;and\;S_{3/2}$ which are shown to be tightly correlated with Kinman's spectral types and the helium abundances obtained from the R-method, respectively. The Z- and Y- abundances are derived from $(B-V)_{1/2}\;and\;S_{3/2}$, respectively, and the latter parameters determine the age class of clusters with help of Dickens' HB type, which is a function of Z. Y and age. For the examined forty two globular clusters the computed range at Z and Y are $1.5{\times}10^{-4}{\leq}Z{\leq}4.5{\times}10^{-2}\;and\;0.23<Y{\leq}0.41$. The age difference between the oldest (HB type 1) and the youngest (HB type 7) clusters is roughly estimated to be $2-4{\times}10^9$ years. Using these four parameters the known anomalous C-M diagrams seem to be reasonably interpreted without taking into account some complicate parameters such as unusually overabundant heavy elements, mass loss and mass spread, etc. The four dimensional scheme strongly suggests the slow successive collapses of the proto-Galaxy rather than a single fast collapse, and by this slow collapse model the inversion of chemical abundance gradient in the Galaxy can be explained. It is also shown that the clump position along the RGB near the HB level removes down to the fainter magnitude as the Z(Y)- abundance increases (decreases).

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1983-1992
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• 2016

Pseudomonas strains isolated from the heavily contaminated Lubin copper mine and Zelazny Most post-flotation waste reservoir in Poland were screened for the presence of integrons. This analysis revealed that two strains carried homologous DNA regions composed of a gene encoding a DNA_BRE_C domain-containing tyrosine recombinase (with no significant sequence similarity to other integrases of integrons) plus a three-component array of putative integron gene cassettes. The predicted gene cassettes encode three putative polypeptides with homology to (i) transmembrane proteins, (ii) GCN5 family acetyltransferases, and (iii) hypothetical proteins of unknown function (homologous proteins are encoded by the gene cassettes of several class 1 integrons). Comparative sequence analyses identified three structural variants of these novel integron-like elements within the sequenced bacterial genomes. Analysis of their distribution revealed that they are found exclusively in strains of the genus Pseudomonas.

• Advances in Computational Design
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• v.7 no.3
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• pp.189-210
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• 2022

Deployable structures have the ability to shift from a compact state to an expanded functional configuration. By extension, reconfigurability is another function that relies on embedded computation and actuators. Linkage-based mechanisms constitute promising systems in the development of deployable and reconfigurable structures with high flexibility and controllability. The present paper investigates the deployment and reconfigurability of modular linkage structures with a pin and a sliding support, the latter connected to a linear motion actuator. An appropriate control sequence consists of stepwise reconfigurations that involve the selective releasing of one intermediate joint in each closed-loop linkage, effectively reducing it to a 1-DOF "effective crank-slider" mechanism. This approach enables low self-weight and reduced energy consumption. A kinematics and finite-element analysis of different linkage systems, in all intermediate reconfiguration steps of a sequence, have been conducted for different lengths and geometrical characteristics of the members, as well as different actuation methods, i.e., direct and cable-driven actuation. The study provides insight into the impact of various structural typological and geometrical factors on the systems' behavior.

• Earthquakes and Structures
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• v.21 no.6
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• pp.627-639
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• 2021

Fragility curves are being more significant as a useful tool for evaluating the relationship between the earthquake intensity measure and the effects of the engineering demand parameter on the buildings. In this paper, the effect of different site conditions on the vulnerability of the structures was examined through the fragility curves taking into account different strength capacities of the precast columns. Thus, typical existing single-story precast RC industrial buildings which were built in Turkey after the year 2000 were examined. The fragility curves for the three typical existing industrial structures were derived from an analytical approach by performing non-linear dynamic analyses considering three different soil conditions. The Park and Ang damage index was used in order to determine the damage level of the members. The spectral acceleration (Sa) was used as the ground motion parameter in the fragility curves. The results indicate that the fragility curves were derived for the structures vary depending on the site conditions. The damage probability of exceedance values increased from stiff site to soft site for any Sa value. This difference increases in long period in examined buildings. In addition, earthquake demand values were calculated by considering the buildings and site conditions, and the effect of the site class on the building damage was evaluated by considering the Mean Damage Ratio parameter (MDR). Achieving fragility curves and MDR curves as a function of spectral acceleration enables a quick and practical risk assessment in existing buildings.

• Journal of Internet Computing and Services
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• v.15 no.6
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• pp.77-84
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• 2014

The purpose of this study is to explore factors affecting the effect of e-learning, and to find out the casual relationship among these factors. Subjects are 2,091 students who have participated in e-learning based class during the period of second semester in 2013. Those of them, 1,732 students response to the survey questions. After gathering data, they are analyzed by using Confirmative Factor Analysis and Structural Equation Modeling. From the result of Confirmative Factor analysis, data have reduced four factors, and are named as four latent variables likes e-learning effect, contents satisfaction, managing assistant factor, and system functional factor. From the result of Structural Equation Modeling, it is known as the relation and impact among factors: (a) "managing assistant factor" affects to "contents satisfaction" directly. (b) "contents satisfaction" affects to "e-learning effect" directly. (c) "system function factor" affects directly to "contents satisfaction", but does not affect directly to "e-learning effect". (d) both "managing assistant factor" and "system function factor" have an indirect effect on "e-learning effect" via "contents satisfaction".

• BMB Reports
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• v.36 no.6
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• pp.603-607
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• 2003

Abstract Plant lipid transfer proteins (LTPs) are a class of proteins whose functions are still unknown. Some are proposed to have antimicrobial activities. To understand whether LTP110, a rice LTP that we previously identified from rice leaves, plays a role in the protection function against some serious rice pathogens, we investigated the antifungal and antibacterial properties of LTP110. A cDNA sequence, encoding the mature peptide of LTP110, was cloned into the Impact-CN prokaryotic expression system. The purified protein was used for an in vitro inhibition test against rice pathogens, Pyricularia oryzae and Xanthomonas oryzae. The results showed that LTP110 inhibited the germination of Pyricularia oryzae spores, and its inhibitory activity decreased in the presence of a divalent cation. This suggests that the antifungal activity is affected by ions in the media; LTP110 only slightly inhibited the growth of Xanthomonas oryzae. However, the addition of LTP110 to cultured Chinese hamster ovarian cells did not retard growth, suggesting that the toxicity of LTP110 is only restricted to some cell types. Its antimicrobial activity is potentially due to interactions between LTP and microbe-specific structures.

• Korean Journal of Veterinary Research
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• v.54 no.3
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• pp.139-146
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• 2014

Jeotgal (salted seafood) has been one of major fermented foods in Korea for long time. Although there are many studies about Jeotgal in various aspects of food, its immunological importance on hosts has not been elucidated yet. In this study, we investigated if several bacteria isolated from Jeotgal may modulate the function of dendritic cells (DCs), powerful antigen-presenting cells equipped with special immunological capabilities. 4 Jeotgal bacteria were selected as representatives and used for experiments. To treat viable DCs, those bacteria were killed at $60^{\circ}C$ for 30 min. The viability of DCs treated with Jeotgal bacteria was verified and two isolates significantly induced high production of interleukin-12, a representative cell-mediated cytokine of DCs. Surface activation and maturation markers (MHC class II, CD40, CD86) of DCs were analyzed by flow cytometer. In addition, the treated DCs showed significantly high lymphocyte stimulatory capability compared to control DCs based on allogeneic mixed lymphocyte reactions. These observations suggest that Jeotgal isolates can function as immunostimulating bacteria in hosts, like Lactobacillus. Taken together, these experimental evidences may broaden the use of Jeotgal isolates in immunological fields in addition to as a fermented food.

• Molecules and Cells
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• v.19 no.3
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• pp.328-333
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• 2005

Small heat shock proteins (sHSPs) are widely distributed, and their function and diversity of structure have been much studied in the field of molecular chaperones. In plants, which frequently have to cope with hostile environments, sHSPs are much more abundant and diverse than in other forms of life. In response to high temperature stress, sHSPs of more than twenty kinds can make up more than 1% of soluble plant proteins. We isolated a genomic clone, NtHSP18.3, from Nicotiana tabacum that encodes the complete open reading frame of a cytosolic class I small heat shock protein. To investigate the function of NtHSP18.3 in vitro, it was overproduced in Escherichia coli and purified. The purified NtHSP18.3 had typical molecular chaperone activity as it protected citrate synthase and luciferase from high temperature-induced aggregation. When E. coli celluar proteins were incubated with NtHSP18.3, a large proportion of the proteins remained soluble at temperatures as high as $70^{\circ}C$. Native gel analysis suggested that NtHSP18.3 is a dodecameric oligomer as the form present and showing molecular chaperone activity at the condition tested. Binding of bis-ANS to the oligomers of NtHSP18.3 indicated that exposure of their hydrophobic surfaces increased as the temperature was raised. Taken together, our data suggested that NtHSP18.3 is a molecular chaperone that functions as a dodecameric complex and possibly in a temperature-induced manner.

• Journal of Internet Computing and Services
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• v.12 no.5
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• pp.21-28
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• 2011

Recently medical and entertainment applications using conducting textile are suggested, but the data of conducting threads are not characterized, classified and verified. Only the data sheet published by manufacturing companies is available. Thus we need to verify the performances of the threading threads in communication. And we need a guideline if the existing communication protocols can be used for the conducting threads communication or the new specific communication protocols have to be developed for the communication. This paper classifies the characteristics of conducting threads made by domestic and overseas companies. Based on the criteria we classified conducting threads into three classes: class A, class B and class C. Further we carried out experiments to verify the adaptability of existing simple serial communication protocols such as RS232. Six different conducting threads are used in experiments and the length of each thread was 0.5m, 1m, 2m and 3m. The data transmission rate and error rate are collected and analyzed. RS485 is very prone to error due to static electricity from human and environment. So it may not be appropriate as long-distance communication protocol up to 12km which is possible in theory. RS232 shows stable and error-less data transmission ability even though every conducting thread didn’t show transmission capability over RS232. USB protocol shows high data rate transmission but the distance cannot be exceeded over 2m. Additionally, USB requires stable power supply. But if the power is supplied through conducting thread, its function is not.