• Title/Summary/Keyword: C-PAM

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Weldline Movement Characteristics for the Warm Deep Drawing of Tailor Welded Blanks (용접 판재의 온간 성형에서의 용접선 이동 특성)

  • Yoo J. S.;Heo Y. M.;Lee S. M.;Seo D. G.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2001.05a
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    • pp.151-155
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    • 2001
  • The purpose of this study is to investigate the weldline movement of the laser welded sheets during the warm deep drawing process. For this investigation, Five steps of temperature ranges, from room temperature to $200^{\circ}C$, and two kinds of thickness combination, 0.8 mm${\times}$1.2 mm and 0.8 mm${\times}$1.6 mm SCP1 material sheets, were adopted. Also, the numerical analysis using the PAM-STAMP has been carried out with the same models as the specimens. As a result the higher temperature was adopted, the less weld-line movement was observed.

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The Analysis of Optimal Cluster Number of Precipitation Region with Dunn Index (Dunn 지수를 이용한 최적 강수지역 군집수 분석)

  • Um, Myoung-Jin;Jeong, Chang-Sam;Nam, Woo-Sung;Jung, Young-Hun;Heo, Jun-Haeng
    • Proceedings of the Korea Water Resources Association Conference
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    • 2011.05a
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    • pp.87-91
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    • 2011
  • 강수는 지역에 따라 발생양상이 매우 다른 자연현상 중 하나이다. 이러한 강수를 효과적으로 분석하여 확률강수량을 산정하기위해서 수문학에서는 다양한 방법이 시도되어 왔다. 우리나라에서는 지점빈도해석을 통한 확률강수량을 주로 사용해왔으나 최근 들어 Hosking and Wallis(1997)가 제안한 지역빈도해석을 활용을 적극 도모 하고 있는 중이다. 이러한 지역빈도해석 기법은 지점빈도해석 기법에 비하여 한정된 강수자료를 활용하는 측면 등 여러 가지 장점을 가진 확률 강수량 산정방법이다. 그러나 이 기법을 적용하여 확률강수량을 산정하기 위해서는 강수의 지역구분을 먼저 수행하여야 한다. 강수지역의 구분을 위해서는 여러 가지 기법이 존재하나 최근에는 Cluster 기법 중 K-means 방법이나 Fuzzy c-means 방법 등을 주로 적용하여 지역구분을 수행하고 있다. 그러나 K-means 방법이나 Fuzzy c-means 방법 등은 산정 방법내에서 최적 군집수를 결정할 수 있는 알고리즘이 없기 때문에 임의적으로 최적 군집수를 결정하여야 한다. 본 연구에서는 이러한 단점을 극복하기 위하여 Cluster 평가지수 중 하나인 Dunn 지수를 이용하여 최적 군집수를 제시하고자 한다. 본 연구에서 강수지역을 구분하기 위하여 적용한 인자는 월 평균 강수량, 연 평균 강수량, 월 최대 강수량, 경도, 위도, 고도 등이며, 이를 K-means, PAM 및 친근도 전파 기법을 통하여 강수지역을 구분하였다. 적정 군집수를 임의적으로 증가시켜 가면서 Dunn 지수를 산정하였다. 산정된 결과를 통하여 최적 군집수를 결정하였다.

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ORF5a Protein of Porcine Reproductive and Respiratory Syndrome Virus is Indispensable for Virus Replication (PRRS 바이러스 ORF5a 단백질의기능학적역할)

  • Oh, Jongsuk;Lee, Changhee
    • Microbiology and Biotechnology Letters
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    • v.43 no.1
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    • pp.1-8
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    • 2015
  • In this study, a DNA-launched reverse genetics system was developed from a type 2 porcine reproductive and respiratory syndrome virus (PRRSV) strain, KNU-12. The complete genome of 15,412 nucleotides was assembled as a single cDNA clone and placed under the eukaryotic CMV promoter. Upon transfection of BHK-tailless pCD163 cells with a full-length cDNA clone, viable and infectious type 2 progeny PRRSV were rescued. The reconstituted virus was found to maintain growth properties similar to those of the parental virus in porcine alveolar macrophage (PAM) cells. With the availability of this type 2 PRRSV infectious clone, we first explored the biological relevance of ORF5a in the PRRSV replication cycle. Therefore, we used a PRRSV reverse genetics system to generate an ORF5a knockout mutant clone by changing the ORF5a translation start codon and introducing a stop codon at the 7th codon of ORF5a. The ORF5a knockout mutant was found to exhibit a lack of infectivity in both BHK-tailless pCD163 and PAM-pCD163 cells, suggesting that inactivation of ORF5a expression is lethal for infectious virus production. In order to restore the ORF5a gene-deleted PRRSV, complementing cell lines were established to stably express the ORF5a protein of PRRSV. ORF5a-expressing cells were capable of supporting the production of the replicationdefective virus, indicating complementation of the impaired ORF5a gene function of PRRSV in trans.

A Study on the Impacts of Paste Type Torrefied Wood Flour Coagulants on Water Ecosystem (반탄화목분 Paste상 응집제의 수생태계 미치는 영향에 관한 연구)

  • YANG, Seung Min;LEE, Seok Eon;PARK, Hae Keum;KANG, Seog Goo
    • Journal of the Korean Wood Science and Technology
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    • v.47 no.6
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    • pp.709-720
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    • 2019
  • Due to global warming and abnormal climate, the incidence and scale of green tracts in rivers and water intake dam are increasing every year. Therefore, in this study, developed eco friendly positively charged Torrefied Wood Flour(TWF) coagulant by reusing wood damaged by blight as a natural material. In order to evaluate the effect of coagulant on water ecosystem, green algae contaminated water was collected and TOC showed high removal rate of 86% ~ 92% under 1% and 5% TWF C-PAM treatment condition. The $NH_3-N$ showed 53% removal efficiency. The average pH of the polluted water was 7.9 in the case of hydrogen ion concentration, and the pH of the treated water was in the range of 6.5 ~ 7.7, It was found to be suitable for water quality standards. In ecotoxicity tests, all the results of the experiment showed that both the number of green algae and that of treated water were not affected by the survival of the daphnia. Therefore, as a result of the analyzing, developed paste type TWF coagulants is considered to be able to remove algae using natural resources.

Factors Affecting the Wintering Habitat of Major Fishery Resources in Southwestern Korean Waters

  • Kim, Jin-Yeong;Choi, Il-Su;Kim, Joo-Il;Choi, Seok-Gwan;Chun, Young-Yull
    • Ocean Science Journal
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    • v.42 no.1
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    • pp.41-48
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    • 2007
  • We investigated the temperature and salinity effects on the major fish species in the wintering grounds based on trawl surveys and oceanographic observations in the southwestern waters of Korea during March-early April in 2002-2003. The influence area of warm Kuroshio water was limited to the southwestern area of Korea in 2003 with a range of $7.7-16.3^{\circ}C$, 32.54-34.70 of salinity, wider than that of 2002. The number of fish species and density of major fish species in 2003 were higher than in 2002. Geographical estimation showed high proportions of species number and catches in the areas around Jeju Islands, southwestern waters and the southeastern coast of Korea. Five species; silver pomfret (Pam pus echinogaster), hairtail (Trichiurus lepturus), anchovy (Engraulis japonicus), Small yellow croaker (Larimichthys polyactis) and yellow goosefish (Lophius litulon) were most abundant, composing above 60% of the total catch in 2002 and 2003. More than 50% of catch in the major fish species were mostly distributed in the range of $9.5-11.0^{\circ}C$ of temperature and 33.1-33.9 of salinity. Non-parametric estimation for the major species showed the 1st mode around $10^{\circ}C$ and the 2nd mode at $8-9^{\circ}C$ in 2002 and $11-14^{\circ}C$ in 2003. Among major fish species, hairtail was principally composed of juveniles, and larger individuals were caught in southeastern waters. These results are considered to be helpful for the area-based fishery management strategy for the wintering grounds of the Yellow Sea and coastal waters of Korea.

Effect of light and sediment grain size on the vertical migration of benthic diatoms

  • Du, Guo Ying;Oak, Jung-Hyun;Li, Hongbo;Chung, Ik-Kyo
    • ALGAE
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    • v.25 no.3
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    • pp.133-140
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    • 2010
  • Using chlorophyll fluorescence, the vertical migration of benthic diatoms responding to light intensity and affected by sediment grain size was studied. Minimal fluorescence ($F_o$) of surface sediment was measured by imaging pulse amplitude modulated (Imaging-PAM) fluorometer, and used to monitor diatom biomass variation in surface sediments. The test diatoms, Amphora coffeaeformis (C. Agardh) K$\ddot{u}$tzing and Cylindrotheca closterium (Ehrenberg) Reimann & Lewin, migrated to the sediment surface under irradiance from 50 to 500 ${\mu}mol$ photons $m^{-2}s^{-1}$. However, the diatoms exhibited no evident increase of surface biomass under dark conditions, and even showed slightly decrease of surface biomass under irradiances over 1,000 ${\mu}mol$ photons $m^{-2}s^{-1}$. The light intensity inducing the maximum surface migration of A. coffeaeformis was 100 ${\mu}mol$ photons $m^{-2}s^{-1}$, while the light intensity producing the same effect for C. closterium was 250 ${\mu}mol$ photons $m^{-2}s^{-1}$. C. closterium showed higher motility than A. coffeaeformis. Faster diatom surfacing was observed in larger grain size sediments (125-335 ${\mu}m$) than smaller ones (63-125 ${\mu}m$). This study confirmed the significant influence of light as a main triggering factor behind migration, indicated the distinct effect of different sediment grain size, and highlighted the species-specific migratory ability.

Operating Criteria of Core Exit Temperature in Nuclear Power Plant with using Channel Statistical Allowance (총채널 불확실도를 적용한 원전 노심출구온도의 운전가능 판정기준)

  • Sung, Je Joong;Joo, Yoon Duk;Ha, Sang Jun
    • Journal of the Korean Society of Safety
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    • v.29 no.6
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    • pp.166-171
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    • 2014
  • Nuclear power plants are equipped with the reactor trip system (RTS) and the engineered safety features actuation system (ESFAS) to improve safety on the normal operation. In the event of the design basis accident (DBA), a various of post accident monitor(PAM)systems support to provide important details (e.g. Containment pressure, temperature and pressure of reactor cooling system and core exit temperature) to determine action of main control room (MCR). Operator should be immediately activated for the accident mitigation with the information. Especially, core exit temperature is a critical parameter because the operating mode converts from normal mode to emergency mode when the temperature of core exit reaches $649^{\circ}C$. In this study, uncertainty which was caused by exterior environment, characteristic of thermocouple/connector and accuracy of calibrator/indicator was evaluated in accordance with ANSI-ISA 67.04. The square root of the sum of square (SRSS) methodology for combining uncertainty terms that are random and independent was used in the synthesis. Every uncertainty that may exist in the hardware which is used to measure the core exit temperature was conservatively applied and the associative relation between the elements of uncertainty was considered simultaneously. As a result of uncertainty evaluation, the channel statistical allowance (CSA) of single channel of core exit temperature was +1.042%Span. The range of uncertainty, -0.35%Span ($-4.05^{\circ}C$) ~ +2.08%Span($24.25^{\circ}C$), was obtained as the operating criteria of core exit temperature.

Single-Base Genome Editing in Corynebacterium glutamicum with the Help of Negative Selection by Target-Mismatched CRISPR/Cpf1

  • Kim, Hyun Ju;Oh, Se Young;Lee, Sang Jun
    • Journal of Microbiology and Biotechnology
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    • v.30 no.10
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    • pp.1583-1591
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    • 2020
  • CRISPR/Cpf1 has emerged as a new CRISPR-based genome editing tool because, in comparison with CRIPSR/Cas9, it has a different T-rich PAM sequence to expand the target DNA sequence. Single-base editing in the microbial genome can be facilitated by oligonucleotide-directed mutagenesis (ODM) followed by negative selection with the CRISPR/Cpf1 system. However, single point mutations aided by Cpf1 negative selection have been rarely reported in Corynebacterium glutamicum. This study aimed to introduce an amber stop codon in crtEb encoding lycopene hydratase, through ODM and Cpf1-mediated negative selection; deficiency of this enzyme causes pink coloration due to lycopene accumulation in C. glutamicum. Consequently, on using double-, triple-, and quadruple-base-mutagenic oligonucleotides, 91.5-95.3% pink cells were obtained among the total live C. glutamicum cells. However, among the negatively selected live cells, 0.6% pink cells were obtained using single-base-mutagenic oligonucleotides, indicating that very few single-base mutations were introduced, possibly owing to mismatch tolerance. This led to the consideration of various target-mismatched crRNAs to prevent the death of single-base-edited cells. Consequently, we obtained 99.7% pink colonies after CRISPR/Cpf1-mediated negative selection using an appropriate single-mismatched crRNA. Furthermore, Sanger sequencing revealed that single-base mutations were successfully edited in the 99.7% of pink cells, while only two of nine among 0.6% of pink cells were correctly edited. The results indicate that the target-mismatched Cpf1 negative selection can assist in efficient and accurate single-base genome editing methods in C. glutamicum.

Maximum concrete stress developed in unconfined flexural RC members

  • Ho, J.C.M.;Pam, H.J.;Peng, J.;Wong, Y.L.
    • Computers and Concrete
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    • v.8 no.2
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    • pp.207-227
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    • 2011
  • In flexural strength design of unconfined reinforced concrete (RC) members, the concrete compressive stress-strain curve is scaled down from the uni-axial stress-strain curve such that the maximum concrete stress adopted in design is less than the uni-axial strength to account for the strain gradient effect. It has been found that the use of this smaller maximum concrete stress will underestimate the flexural strength of unconfined RC members although the safety factors for materials are taken as unity. Herein, in order to investigate the effect of strain gradient on the maximum concrete stress that can be developed in unconfined flexural RC members, several pairs of plain concrete (PC) and RC inverted T-shaped specimens were fabricated and tested under concentric and eccentric loads. From the test results, the maximum concrete stress developed in the eccentric specimens under strain gradient is determined by the modified concrete stress-strain curve obtained from the counterpart concentric specimens based on axial load and moment equilibriums. Based on that, a pair of equivalent rectangular concrete stress block parameters for the purpose of flexural strength design of unconfined RC members is determined.

Research for the Analytical Method of Various Pesticides in Raw Milk by Gas Chromatography-Mass Spectrometry (GC/MSD를 사용한 원유 내 잔류농약의 분석법 연구)

  • Oh, N.S.;Shin, Y.K.;Baick, S.C.
    • Food Science of Animal Resources
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    • v.29 no.4
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    • pp.482-486
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    • 2009
  • The aim of this study was to optimize a simple, fast, and economical analysis procedure for the determination of 16 different pesticides in raw milk via GC/MSD. Analyses were performed via gas chromatography with electron impact mass spectrometric detection in the selected ion monitoring mode (GC/MSD-SIM) using Pentachloronitrobenzene as the internal standard. The modified sample preparation methodology was based on the Pesticide Analytical Manual (PAM) of the FDA concerning fat extraction, ACN-ether partitioning, and clean-up of the Sep-Pak florisil cartridge. The modified methodology for the determination of the 16 pesticides was validated. The range of LOQs of the 16 pesticides was likely three times lower than their Maximum Residence Levels (MRLs). The recoveries of most of the pesticides were acceptable at the fortification levels of 0.5 and 1.0 ${\mu}g/mL$ and their RSD (%) level was less than 20%. None of the 16 pesticides were detected in the selected raw milk samples.