• Journal of Forest and Environmental Science
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• v.40 no.1
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• pp.9-14
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• 2024

Quantitative evaluation of nutrient status and stoichiometry on the forest floor is a good indicator of litter quality in various vegetation types. This study was conducted to determine the effects of vegetation type on the nutrient concentration and stoichiometry of forest floors at a regional scale. Forest floor samples were collected from four vegetation types of evergreen coniferous forests including Cryptomeria japonica, Chamaecyparis obtusa, evergreen broadleaf, and bamboo spp. forests in southern Korea. The dry weight of the forest floor was higher in the C. japonica and C. obtusa forests than in the evergreen broadleaf and bamboo forests. The mean carbon (C) concentrations of the forest floor were highest in the broadleaf forest, followed by the bamboo forest, C. japonica and C. obtusa forests. Mean nitrogen (N) and phosphorous (P) concentrations in the the coniferous forests were lower than those in the broadleaf and bamboo forests. The mean C:N ratio was the highest in C. obtusa forest (118±25), followed by C. japonica (66±6), evergreen broadleaf (41±1), and bamboo (30±1) forests. However, C:P and N:P ratios were lower in the coniferous forests than in the broadleaf forest indicating that the stoichiometry of the forest floor varies across vegetation types. The C, N, and P stocks on the forest floor were higher in the C. obtusa forest than in the broadleaf or bamboo forests. These results highlight that vegetation type-dependent stoichiometric ratio is an useful indicator for understanding interspecific difference in quality and quantity of the forest floor.

• Journal of Soil and Groundwater Environment
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• v.9 no.4
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• pp.42-51
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• 2004

Peat humin(p-Humin), humic acid(p-HA) and fulvic acid(p-FA) were isolated from Canadian Sphagnum peat moss by dissolution in 0.1M NaOH followed by acid precipitation. After purification cycles, they are characterized for their elemental compositions and, acid/base properties. Functionalities and carbon structures of the humic fractions were also characterized using FT-IR and solid state $^{13}C$-NMR spectroscopy. Those results are compared with one another and with soil humic substances from literatures. Main purpose of this study was to present a chemical and spectroscopic characterization data of humic substance from peat moss needed to evaluate its environmental applicability. The relative proportions of the p-Humin, p-HA and p-FA in the peat moss was $76\%,\;18\%,\;and\;3\%$, respectively, based on the total organic matter content ($957{\pm}32\;g/kg$). Elemental composition of p-Humin were found to be $C_{1.00}H_{1.52}O_{0.79}N_{0.01}$ and had higher H/C and (N+O)/C ratio compared to those of p-HA($C_{1.00}H_{1.09}O_{0.51}N_{0.02}$) and p-FA($C_{1.00}H_{1.08}O_{0.65}N_{0.01}$). Based on the analysis of pH titration data, there are two different types of acidic functional groups in the peat moss and its humic fractions and their proton exchange capacities(PEC, meq/g) were in the order p-FA(4.91) >p-HA(4.09) >p-Humin(2.38). IR spectroscopic results showed that the functionalities of the peat moss humic molecules are similar to those of soil humic substances, and carboxylic acid(-COOH) is main function group providing metal binding sites for Cd(II) sorption. Spectral features obtained from $^{13}C$-NMR indicated that peat moss humic molecules have rather lower degree of humification, and that important structural differences exist between p-Humin and soluble humic fractions(p-HA and p-FA).

• Communications of the Korean Mathematical Society
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• v.34 no.1
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• pp.279-286
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• 2019

Complex Grassmann manifolds $G_{n,k}$ are a generalization of complex projective spaces and have many important features some of which are captured by the $Pl{\ddot{u}}cker$ embedding $f:G_{n,k}{\rightarrow}{\mathbb{C}}P^{N-1}$ where $N=\(^n_k\)$. The problem of existence of cross sections of fibrations can be studied using the Gottlieb group. In a more generalized context one can use the relative evaluation subgroup of a map to describe the cohomology of smooth fiber bundles with fiber the (complex) Grassmann manifold $G_{n,k}$. Our interest lies in making use of techniques of rational homotopy theory to address problems and questions involving applications of Gottlieb groups in general. In this paper, we construct the Sullivan minimal model of the (complex) Grassmann manifold $G_{n,k}$ for $2{\leq}k<n$, and we compute the rational evaluation subgroup of the embedding $f:G_{n,k}{\rightarrow}{\mathbb{C}}P^{N-1}$. We show that, for the Sullivan model ${\phi}:A{\rightarrow}B$, where A and B are the Sullivan minimal models of ${\mathbb{C}}P^{N-1}$ and $G_{n,k}$ respectively, the evaluation subgroup $G_n(A,B;{\phi})$ of ${\phi}$ is generated by a single element and the relative evaluation subgroup $G^{rel}_n(A,B;{\phi})$ is zero. The triviality of the relative evaluation subgroup has its application in studying fibrations with fibre the (complex) Grassmann manifold.

• Bulletin of Food Technology
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• v.8 no.4
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• pp.179-191
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• 1995

Thermoanaerobacter ethanolicus유래 thermophilic amylopullulanase의 thermophilicity와 thermostability의 기작을 규명하기 위하여 N-말단과 C-말단으로부터 nested deletion mutatnt와 sitedirected mutagenesis등에 의한 변이효소를 제조, 분석하였다. 이러한 까다로운 변이효소를 제조하여 amylopullulanase의 특정부위가 효소의 thermophilicity와 thermostability에 관여하고 있는 것을 확인했다. N-말단의 start amino acid에서 194와 324 아미노산잔기에 이르는 부위 (TPR)가 이 효소의 높은 최적반응온도의 유지에 관련되어 있고 1102와 1224잔기에 이르는 부위 (TSR)는 thermodenaturation이 잘 일어나지 않도록 하고 있었다. 야생형 amylopullulanase (Apu), 변이효소중 ApuN342와 ApuN106/C379는 비슷한 효소비활성과 Km값을 가지고 있었다. TPR부위의 site-directed mutagenesis에 의한 변이효소중 P240A (proline$\rightarrow$alanine), P244A, P240A-P244A는 야생형의 최적반응온도 $80^{\circ}C$와 똑같았지만 효소의 열안전성(반감기)는 $85^{\circ}C$에서 21, 105, 128분을 보여 주었다. TSR에서의 변이효소중 P1159A, P1202A는 열안전성은 비슷하였지만 반응최적온도는 $85^{\circ}C$와 $90^{\circ}C$로 야생형 Apu보다 오히려 높아졌다. 따라서 proline은 분자내에서 thermophilicity와 thermostability를 항상 증가시키는 쪽으로 영향을 주지 않는 것으로 보인다. Proline은 그 위치와 주위의 다른 아미노산잔기와 같이 종합적으로 분자의 구조에 영향을 미치고 있는 것으로 판단된다.

• Korean Journal of Animal Reproduction
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• v.24 no.1
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• pp.115-120
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• 2000

We have investigated the effect of short-term immobilization stress on the serum concentrations of cortisol and DHEAS in BALB/c male mice. Serum cortisol and DHEAS concentrations were measured by radioimmunoassay(RIA). We found there were significantly increased in the cortisol levels in 30 min-stressed group(Ⅰ-30N) compared with control(C) group (p＜0.01), and also increased with significance in 120 min-stressed group(I-120N) compared with C group(p＜0.01). Cortisol concentrations were significantly increased in both 30 min-stressed group(Ⅰ-30T), and 120 min-stressed group(Ⅰ-120T) compared with C group(p＜0.01). The sustained increase of cortisol levels were observed in both SG treated and SG non-treated group. Serum cortisol levels were lower in SG treated group than SG non-treated group with significance(p＜0.01). By contrast, DHEAS levels were slightly decreased without significance in Ⅰ-30N, but significantly decreased in Ⅰ-120N compared with C group(p＜0.01). There were slightly decreased in the DHEAS levels in Ⅰ-30T, but significantly decreased in Ⅰ-120T compared with C group(p＜0.01). However, SG treatment did not induce any significant changes of DHEAS levels in both 30 min and 120 min-stressed group. Though short-term immobilization stress, the continuous decline of DHEAS levels were observed. Therefore, these results show that short-term immobilization stress affects the serum concentrations of cortisol and DHEAS in mice.

• Korean Journal of Organic Agriculture
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• v.25 no.1
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• pp.219-231
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• 2017

The aim of this study was to determine the effects of feeding of different organic diets (based on fatty acid contents) on CLA and fatty acid concentration of raw milk. Total two hundred and ninety Holstein cows were divided into control, treatment 1 and treatment 2 groups according to the parity and milk yield. Control diet was contained higher C16:00, C18:2 and SFA. Treatment 1 diet was higher in C18:1, C18:2, UFA and treatment 2 diet was higher in MUFA and C18:3 and PUFA. The results indicated that the C16:0 concentration in raw milk was greater in the group of treatment 2 than in control and treatment 1 (p<0.05). The concentration of CLA in treatment 2 was higher compared to treatment 1 and control groups (p<0.05). The ration of n-3/n-6 was higher in treatment 2 group compared with control and treatment 1 (p<0.05). In conclusion, feeding 100% of grass feed for dairy cows increases CLA and n-3 content in milk. From now on, basis on our study, development of functional milk will increase a health of children and old people.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.343-347
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• 2003

Power of loess ball on nitrogen and phosphorous removal in wastewater treatment were investigated. flow line A ( anaerobic${\rightarrow}$oxic${\rightarrow}$anoxic(organic source methanol)${\rightarrow}$p-absorption) showed the results of T-P 0.5, T-N 1.0, and COD 10ppm bellow, and flow line B ( oxic${\rightarrow}$anoxic, organic source: methanol${\rightarrow}$p-absorption) showed the results of T-P 0.3, T-N 5.0, and COD 15 ppm bellow. flow line C ( anaerobic${\rightarrow}$oxic${\rightarrow}$anoxic, organic source: wastewater ${\rightarrow}$ p-absorption) showed the results of T-P 0.6, T-N 10, and COD 15 ppm bellow, and flow line D ( oxic${\rightarrow}$anoxic, organic source: methanol${\rightarrow}$p-absorption) showed the results of T-P 1, T-N 8m, and COD 20 ppm bellow. So the results of these experiments showed the probability of loess ball in wastewater treatment.

• Honam Mathematical Journal
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• v.2 no.1
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• pp.1-8
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• 1980

논문(論文)[3] (본(本) 논문(論文) 제1부(第1部))에서 미분가능다양체(微分可能多樣體) M 위의 (n-1)차(次) 미분형식(微分型式) ${\beta}^{(n-1)}$이 Compact인 Carrier를 가지면 ${\int}d{\beta}^{(n-1)}=0$이며, (p-1)차(次) 미분형식(微分型式) ${\beta}^{(p-1)}$과 p차(次) 미분가능쇄(微分可能쇄鎖) $C^{(p)}=\Sigma\limits_ik_iS_i{^{(p)}}$에 대(對)하여 ${\int\limits_{c^{(p)}}}d{\beta}^{(p-1)}={\int\limits_{{\partial}{c}^{(p)}}}{\beta}^{(p-1)}$이 성립(成立) (Stokes 정리(定理)의 일반화(一般化))⋯등(等) M위의 적분(積分)에 관한 여러 가지 성질(性質)들을 구명(究明)하였다. 이 성질(性質)들을 토태(土台)로 하여 본(本) 논문(論文)에서는; 제2절(第2節)에서 미분가능다양체(微分可能多樣體) M위의 Lie 도함수(導函數)의 정의(定義)와 Lie적분(微分)에 관(關)한 여러가지 성질(性質)들을 고찰(考察)하고, 제3절(第3節)에서 div X와 Laplace 작용소(作用素) ${\Delta}f$의 정의(定義) 및 실(實) n차원(次元) 가부호미분가능(可符號微分可能) 다양체(多樣體) M 위에서의 divX와 ${\Delta}f$의 적분(積分)에 관(關)한 성질(性質), 즉(卽) $V=\sqrt{{\mid}g{\mid}}dx^1{\Lambda}{\cdots}{\Lambda}dx^n{\in}A^n(M)$에 대(對)하여 $$\int_MdivXV\limits=\int_M{\Delta}fv=0$$인 관계(關係)가 성립(成立)함을 구명(究明)한다. (정리(定理) 3.3)

• Journal of Korean Society of Environmental Engineers
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• v.28 no.3
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• pp.286-291
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• 2006

We studied possibility of mixing treatment of livestock wastewater and sewage using Phanerochaete chrysosporium PSBL-1. Our study showed that 97.6% of SS and 95% of T-P removal efficiency was achieved when 2 mL BF02(a coagulant) and 100 mL C-210EL(a cationic polymer) were added to the mixture(2:1, v/v) of livestock wastewater and sewage. We studied treatment characteristic of Phanerochaete chrysosporium PSBL-1, after were mixed pretreated wastewater and sewage by dillution ten times about livestock wastewater. The removal efficiency of NBDCOD(non-biodegradable COD), $NH_3-N$ and T-N was increased according to increase of pH. That is, T-N concentration of effluent was satisfied 60 mg/L by drain water waterqulity standard of livestock wastewater public treatment facilities with 35 mg/L from a lapse of five days at pH 6.7, 51 mg/L from a lapse of three days at pH 8 and 33 mg/L from a lapse of one day at pH 10. Moreover $COD_{Mn}$ concentration of effluent was satisfied 40 mg/L by drain water waterqulity standard of livestock wastewater public treatment facilities after a laps of one day at all pH. Organics and nitrogen concentrations of effluent were higher case with addition of V.A.(veratryl alcohol) than case without addition of V.A.(veratryl alcohol). $COD_{Mn}$ concentration of effluent satisfied drain water qulity standard of livestock wastewater public treatment facilities from a lapse of one day, when C/N rate(3:1) of influent was not controled. T-N satisfied that from a lapse of two days, when C/N rate was controled with $4{\sim}6$.

• Parasites, Hosts and Diseases
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• v.34 no.2
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• pp.135-142
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• 1996

Antigenic domain of jai or surface protein (p30) of Toxoplosmc Sondii was analyzed after polymerase chain reaction (PCR) of its gene fragments. Hydrophilic or hydrophobic moiety of amino acid sequences were expressed as glutathione S-transferase (G57) fusion proteins. Fragments of p30 gene were as follows: 737, total p30 open reading frame (ORF) ; S28, total ORF excluding N-terminal signal sequence and C-terminal hydrophobic sequence; Al9, N-terminal 2/3 parts of A28; A19, N-terminal 2/3 of S28; P9, C-terminal 2/3 part of S28; Z9. middle 1/3 of S28; and 29, C-terminal 1/3 of S28. respectively. Primer of each fragment was synthesized to include clamp sequence of EcoR I restriction site. PCR amplified DNA was inserted info GST (26 kDa) expression vector, PGEX-47-1 to transform into Escheri,hia coei (.JM105 strain). G57 fusion proteins were expressed with IPTG induction as 63. 54, 45, 45, 35, 36. and 35 kDa proteins measured by SDS-PAGE. Each fusion protein was confirmed with G57 detection kit. Western blot analysis with the serum of a toxoplasmosis patient revealed antigenicity in proteins expressed by T37. S28, and Al9 but not those by Pl8. X9, Y10, and Z9. Antigenicity of p30 seems to be located either in N-terminal 115 part in the presence of middle 1/3 part or in the oligopeptides between margins of the first and second 1/3 parts.