• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.7
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• pp.645-652
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• 2015

In this study, a method for determining Ramberg-Osgood constants for leak-before-break evaluation was investigated. The Ramberg-Osgood constants were calculated for SA312, TP316, and SA-508 Gr.1a in an operating temperature of $316^{\circ}C$. Incremental plasticity, using stress-strain data obtained from experiment, and deformation plasticity, using the Ramberg-Osgood constants, were considered in a finite element analysis. Using incremental plasticity and deformation plasticity, J-integrals and crack opening displacement values were calculated and compared. By comparing the results of incremental plasticity and deformation plasticity, a suitable method for determining Ramberg-Osgood constants for leak-before-break evaluation was confirmed.

• Corrosion Science and Technology
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• v.3 no.2
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• pp.59-66
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• 2004

In construction of new ships and large steel bridges in Korea, pre-construction primers (PCP), also known as shop primer, are routinely used and retained as an integral part of the protective coating system. Retention of PCP's can significantly reduce building schedule and cost. Retaining PCP through the so-called "sweep blasting" procedure eliminates or minimizes the necessity of a second blast operation, thus shortening overall schedule as well as reducing labor cost and hazardous waste disposal cost. This study evaluates the feasibility of retaining PCP as the part of primer for high performance protective coating systems applied to ships' hull, bottom and ballast tanks. Upon proving that the retention of the PCP is a viable option, the process of coating application can he improved significantly in terms of cost and working schedule of new ships and large steel bridges. Results indicate that use of the PCP via sweeping blasting in conjunction with standard high performance protective coating systems does not degrade the overall performance of the coating systems. At the same time, it is also highly recommended that the secondary surface preparation should consist of grit blasting of weld burnt and other damaged areas to SSPC SP-IO grade (Sa 2.5 Gr.), Near White Blast Cleaning with proper application and attention to detail.

• Journal of Microbiology and Biotechnology
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• v.27 no.12
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• pp.2190-2198
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• 2017

Thermoactinomyces sp. strain YT06 was isolated from poultry compost and observed to degrade integral chicken feathers completely at $60^{\circ}C$, resulting in the formation of 3.24 mg/ml of free amino acids from 50 ml of culture containing 10 g/l chicken feathers. Strain YT06 could grow and secrete keratinase using feather as the only carbon and nitrogen sources without other supplement, but complementation of 10 g/l sucrose and 4 g/l $NaNO_3$ increased the production of the keratinolytic enzyme. The maximum protease activity obtained was 110 U/ml and for keratinase was 42 U/ml. The keratinase maintained active status over a broad pH (pH 8-11) and temperature ($60-75^{\circ}C$). It was inhibited by serine protease inhibitors and most metal ions; however, it could be stimulated by $Mn^{2+}$ and the surfactant Tween-20. A reductive agent (${\beta}$-mercaptoethanol) was observed to cleave the disulfide bond of keratin and improve the access of the enzyme to the keratinaceous substrate. Zymogram analysis showed that strain YT06 primarily secreted keratinase with a molecular mass of approximately 35 kDa. The active band was assessed by MALDI-TOF mass spectrometry and was observed to be completely identical to an alkaline serine protease from Thermoactinomyces sp. Gus2-1. Thermoactinomyces sp. strain YT06 shows great potential as a novel candidate in enzymatic processing of hard-to-degrade proteins into high-value products, such as keratinous wastes.

• Journal of the Korean Society of Costume
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• v.65 no.6
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• pp.1-14
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• 2015

This study is aims to study the background of how the shape of the wooden shoes developed and worn by Koreans and Japanese in the ancient times by sorting and examining the types and shapes. Relevant bibliography, which are mainly related to the excavated wooden shoes from 3 B.C.E to C.E. 8., were used as research materials. The formation of wooden shoes required easy access to raw materials -which is trees- for production. According to the analysis, both Korea and Japan made the flat wooden shoes using cuboid wood. Both countries bore a hole on a specific location to distinguish the right foot and left foot, however the style of the heel was different in the two countries. The slip-on(Undu-hyeong) wooden shoes were also common in both countries. The slip-on had no-heels and was made by digging-out a piece of the cuboid wood. Some slip-ons made by the Japanese had furrows on the bottom, and they were known to make different types of the slip-on wooden shoes depending on the purpose. Observation of the wearing methods show that commonality can be found between the wooden shoes of Korea and Japan, and this indicates that cultural exchanges between the Silla/Baekje and Japan took place from the 4th to the 6th century. Also, the flat wooden shoes in Japan developed rapidly, as the shoes became an integral part of its life and culture, which was closely tied to agriculture. Eventually, due to the difference in climate and life-style, the slip-ons became the primary type of wooden shoes in Korea, while the flats became the main type of wooden shoes in Japan. It is, however, clear that as the relationship between the two countries became closer, the cultural exchanges regarding the wooden shoes were considerable.

• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1744-1754
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• 2014

The hepatitis C virus (HCV) RNA genome is replicated by an RNA replicase complex (RC) consisting of cellular proteins and viral nonstructural (NS) proteins, including NS5B, an RNA-dependent RNA polymerase (RdRp) and key enzyme for viral RNA genome replication. The HCV RC is known to be associated with an intracellular membrane structure, but the cellular components of the RC and their roles in the formation of the HCV RC have not been well characterized. In this study, we took a proteomic approach to identify stomatin, a member of the integral proteins of lipid rafts, as a cellular protein interacting with HCV NS5B. Co-immunoprecipitation and co-localization studies confirmed the interaction between stomatin and NS5B. We demonstrated that the subcellular fraction containing viral NS proteins and stomatin displays RdRp activity. Membrane flotation assays with the HCV genome replication-competent subcellular fraction revealed that the HCV RdRp and stomatin are associated with the lipid raft-like domain of membranous structures. Stomatin silencing by RNA interference led to the release of NS5B from the detergent-resistant membrane, thereby inhibiting HCV replication in both HCV subgenomic replicon-harboring cells and HCV-infected cells. Our results identify stomatin as a cellular protein that plays a role in the formation of an enzymatically active HCV RC on a detergent-resistant membrane structure.

• Mycobiology
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• v.42 no.2
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• pp.167-173
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• 2014

A ${\beta}$-glucan synthase gene was isolated from the genomic DNA of polypore mushroom Sparassis crispa, which reportedly produces unusually high amount of soluble ${\beta}$-1,3-glucan (${\beta}$-glucan). Sequencing and subsequent open reading frame analysis of the isolated gene revealed that the gene (5,502 bp) consisted of 10 exons separated by nine introns. The predicted mRNA encoded a ${\beta}$-glucan synthase protein, consisting of 1,576 amino acid residues. Comparison of the predicted protein sequence with multiple fungal ${\beta}$-glucan synthases estimated that the isolated gene contained a complete N-terminus but was lacking approximately 70 amino acid residues in the C-terminus. Fungal ${\beta}$-glucan synthases are integral membrane proteins, containing the two catalytic and two transmembrane domains. The lacking C-terminal part of S. crispa ${\beta}$-glucan synthase was estimated to include catalytically insignificant transmembrane ${\alpha}$-helices and loops. Sequence analysis of 101 fungal ${\beta}$-glucan synthases, obtained from public databases, revealed that the ${\beta}$-glucan synthases with various fungal origins were categorized into corresponding fungal groups in the classification system. Interestingly, mushrooms belonging to the class Agaricomycetes were found to contain two distinct types (Type I and II) of ${\beta}$-glucan synthases with the type-specific sequence signatures in the loop regions. S. crispa ${\beta}$-glucan synthase in this study belonged to Type II family, meaning Type I ${\beta}$-glucan synthase is expected to be discovered in S. crispa. The high productivity of soluble ${\beta}$-glucan was not explained but detailed biochemical studies on the catalytic loop domain in the S. crispa ${\beta}$-glucan synthase will provide better explanations.

• Journal of the Korean Institute of Landscape Architecture
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• v.33 no.4 s.111
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• pp.97-107
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• 2005

This study was carried out to construct accurate and scientific system of assessment indicators in selection of National Trust conservation areas, which was new concept of domestic environment movement and offer the raw data of new analytic method by introducing the fuzzy theory and weight for overcoming the uncertainty of ranking decision. To transform the Likert's scale granted to assessment indicators into the type of triangular fuzzy number(a, b, c), there was conversion to each minimum(a), median(b), and maximum(c) in applying membership function, and in using the center of gravity and eigenvalue, there was to decide the ranking. The rankings of converted values applied a mean importance and weight were confirmed that they were generally changed. Therefore, the ranking decision was better to accomplish objective and rational ranking decision by applying weight that was calculated in grouping of indicator than to judge the singular concept and to be useful in assessment of diverse National Trust site. In the future, because AHP, which was general method of calculating weight, was lacked, there was to understand the critical point to fix a pertinent weight, and to carry out the study applying engineering concept like fuzzy integral using $\lambda-measure$.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.35S no.12
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• pp.36-45
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• 1998

In this paper, a method of on-line PI gain-tuninng is proposed for the speed control of brushless D.C. motor by investigating the pattern of input and output without estimating parameter. Proportional gain is tuned in the process to obtain a fast speed response by supplying the maximum constant input. And integral gain is appropriately tuned in the process of proportional control so that the response may be stably converged and the overshoot may be prevented. Therefore because both control and gain-tuning are executed concurrently, additional works that estimate parameters and so on aren't required in the proposed method. In the proposed method, both fast-response and overshoot problem are well solved, and it is more useful and convenient than existing auto-tuning methods in the speed control of D.C. motor. It is illustrated by simulations and experimental results that the proposed method is useful and stable.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.40 no.8
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• pp.693-701
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• 2016

In this study, notch defects are evaluated using fracture mechanics. To understand the effects of notch defects, FE analysis is conducted to predict the limit load and J-integral for middle-cracked and single-edge cracked plates with various sizes of notch under tension and bending. As the radius of the notch increases, the energy release rate also increases, although the limit load remains constant. The values of fracture toughness($J_{IC}$) of SM490A are determined for various notch radii through FE simulation instead of conducting an experiment. As the radius of the notch increases, the energy release rate also increases, together with a more significant increase in fracture toughness. To conclude, as the notch radius increases, the resistance to crack propagation also increases.

• Journal of Korean Society of Forest Science
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• v.90 no.3
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• pp.324-330
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• 2001

This study was carried out to develop prediction model for fill slope failure of forest road in igneous rock area using fuzzy theory which is non-linear model. The results were summarized as follows. The importance weight of factors on fill slope failure was ranked in the order of fill slope length, fill slope gradient, soil type, aspect, road position and longitudinal slope form. The degree of potential slope failure was high mainly under the such conditions as fill slope length greater than 8m, fill slope gradients steeper than $40^{\circ}$, constituent material with weathered rock, aspect of NE and road on ridge position. The optimal prediction model was developed with 0.15 of optimal coefficient(c) and 3.1165 of ${\lambda}$-value when fuzzy integral value of slope failure possibility is more than 0.5. And the discriminant accuracy was 86.8%, which shows the high availability for discrimination.