• Title/Summary/Keyword: Burst assembly

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Stress Variation Characteristics of a High-Pressure Hose with Respect to Wire Braid Angle (강선의 편조각도에 따른 고압호스의 응력변화 특성)

  • Kim, H.J.;Koh, S.W.;Kim, B.T.
    • Journal of Power System Engineering
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    • v.9 no.3
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    • pp.71-78
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    • 2005
  • A high-pressure hose includes rebar layers of the synthetic fiber such as nylon or a steel wire to control internal pressure. The hose assembly is manufactured through the swaging process to clamp the hose into the metal fittings. Usually, the hose behavior is affected by the resultant of the longitudinal and circumferential forces produced by the internal pressure. The rebar layers can appear the most ideal rebar effect when they are arranged to the same direction as the resultant force. The braid angle applied in the rebar layers is an important factor in determining ultimate burst pressure and overall hose life. Failure can occur on the contacted parts of a hose with the metal fittings under severe operating conditions such as high pressure and temperature of the inner fluid. In this paper, the mechanical behavior between the hose and the metal fittings during the swaging process and the stress variation characteristics of a high-pressure hose under a constant applied pressure are analyzed with respect to the braid angle of steel wire using the finite element method.

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Development of Selectable Vector Plasmid in Bacteriophage P2-P4 System and Its Stability (박테리오파지 P2-P4 시스템을 위한 벡터 플라스미드 개발과 안정성)

  • Kim, Kyoung-Jin
    • Korean Journal of Microbiology
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    • v.34 no.4
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    • pp.236-242
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    • 1998
  • While bacteriophage P2-P4 system is very useful experimental tool for the study of viral capsid assembly, there is no useful plasmid vector for the DNA manipulation in bacteriophage P2-P4 system. In this study, a new vector plasmid, P4 ash8 (sid71) kmr, was constructed by swapping the non-essential region of P4 DNA for kanamycin resistance(kmr) gene cassette of plasmid pUC4-K. P4 ash8 sid71 was starting material for the construction, since it tends to be maintained as a plasmid in the absence of the helper phage. The total size of this chimera was designed to be packaged into P4 or P2 size heads with induction by P2 infection. The conversion of plasmid P4 ash8 (sid71) kmr to bacteriophage was proved by burst size determination experiment and CsCl buoyant equilibrium density gradient experiment. Integrase destructed P4 derivative, P4 ash8 sid71 kmr intS, was able to be constructed easily by in vitro DNA manipulation of P4 ash8 sid71 kmr. The plasmid stability experiment with P4 ash8 sid71 kmr if/tS showed that the integrase of P4 affects the stable maintenance of plasmid P4 state.

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