• Journal of Microbiology
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• v.44 no.5
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• pp.530-536
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• 2006

Bacteriophage P4, a satellite phage of coliphage P2, is a very useful experimental tool for the study of viral capsid assembly and cos-cleavage. For an in vitro cos-cleavage reaction study of the P2-P4 system, new shortened and selectable markers containing P4 derivative plasm ids were designed as a substrate molecules. They were constructed by swapping the non-essential segment of P4 DNA for either the kanamycin resistance (kmr) gene or the ampicillin resistance (apr) gene. The size of the genomes of the resulting markers were 82% (P4 ash8 delRI:: kmr) and 79% (P4 ash8 delRI:: apr) of the wild type P4 genome. To determine the lower limit of genome size that could be packaged into the small P4-size bead, these shortened P4 plasmids were converted to phage particles with infection of the helper phage P2. The conversion of plasmid P4 derivatives to bacteriophage particles was verified by the heat stability test and the burst size determination experiment. CsCl buoyant equilibrium density gradient experiments confirmed not only the genome size of the viable phage form of shortened P4 derivatives, but also their packaging into the small P4-size head. P4 ash8 delRI:: apr turned out to be the smallest P4 genome that can be packaged into P4-sized head.

• The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.8 no.4
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• pp.38-45
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• 1994

It is very imprtant and necessary to predict luminous enviroment in an interior space. This paper has de scribed about energy saving and evaluated interior visual environment in a office building having on/off turning control lighting system utilizing daylight. In order to predict the interior varior illumination distribution, the scale model w a m~a de and examined under various conditions, such ad difference of window glass, and color pattern of wall, floor, and also ceiling lighting system type. Ths paper suggests that basic fundamental data of lighting design ~rformancein the concept and schematic stages of design.

• The Plant Pathology Journal
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• v.34 no.1
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• pp.59-64
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• 2018

Bacteriophages of Acidovorax citrulli, the causal agent of bacterial fruit blotch, were isolated from 39 watermelon, pumpkin, and cucumber leaf samples collected from various regions of Korea and tested against 18 A. citrulli strains. Among the six phages isolated, ACP17 forms the largest plaque, and exhibits the morphology of phages in the Myoviridae family with a head diameter of $100{\pm}5nm$ and tail length of $150{\pm}5nm$. ACP17 has eclipse and latent periods of $25{\pm}5min$ and $50{\pm}5min$, respectively, and a burst size of 120. The genome of ACP17 is 156,281 base pairs with a G + C content of 58.7%, 263 open reading frames, and 4 transfer RNA genes. Blast search and phylogenetic analysis of the major capsid protein showed that ACP17 has limited homology to two Stentrophomonas phages, suggesting that ACP17 is a new type of Myoviridae isolated from A. citrulli.

• Journal of the Korean Society for Precision Engineering
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• v.7 no.3
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• pp.75-82
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• 1990

Automatic monitoring of the cutting conditions is one of the most improtant technologies in machining. In this study, the feasibility in applying acoustic emission(AE) signals for the in-process detection of endmill wear and fracture has been investigated by performing experimental test on the NC vertical milling machine with SM45C for specimen. As the results of detecting and analyzing AE signals on various cutting conditions, the followings have confirmed. (1) The RMS value of acoustic emission is related sensitively to the cutting velocity, but is not affected largely by feed rate. (2) The burst type AE signals of high level have been observed when removing chips distorderly and discontinuously. (3) When the RMS value grows up rapidly due to the increase of wear the endmill are generally broken or fractured, but when the endmills fracture at the conditions of smooth chip-flow or built-up-edge(BUE) occurred frequently, the rapid change of the RMS arenot found. And it is expected that this technigue will be quite useful for in-process sensing of tool wear and fracture.

• BMB Reports
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• v.30 no.2
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• pp.89-94
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• 1997

In this study, during the activation of neutrophil responses by sodium fluoride. involvement of protein tyrosine kinase was studied. Respiratory burst lysosomal enzyme release and elevation of $[Ca^{2+}]_i$stimulated by sodium fluoride in neutrophils were inhibited by protein kinase inhibitors, genistein and tyrphostin. The inhibitory effect of genistein and tyrphostin on superoxide and $H_{2}O_{2}$ production was less than that of protein kinase C inhibitors, staurosporine and H-7. Staurosporine and H-7 had little or no effect on the release of myeloperoxidase and acid phosphatase stimulated by sodium fluoride. EGTA and verapamil inhibited the elevation of $[Ca^{2+}]_i$ evoked by sodium fluoride. The inhibitory effect of staurosporine on the elevation of $[Ca^{2+}]_i$ was less than that of genistein. Phorbol 12-myristate 13-acetate (PMA)-stimulated superoxide production, which is sensitive to staurosporine, was further enhanced by genistein, whereas the stimulatory action of PMA on myeloperoxidase release was inhibited by genistein. A pretreatment of neutrophils with PMA signifcantly attenuated sodium fluoride-evoked elevation of $[Ca^{2+}]_i$ These results suggest that protein tyrosine kinase may be involved in the activation process of neutrophil responses due to direct stimulation of guanine nucleotide regulatory proteins. In neutrophil responses, PMA-stimulated neutrophils appear to show a different type of inhibition of protein tyrosine kinase.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.41 no.4
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• pp.65-72
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• 2009

We investigated the effect of polyelectrolyte types in Layer-by-Layer multilayering and furnish combination on physical properties of paper. Handsheets were made from pulp fibers with different polyelectrolytes composition, and their density, formation, tensile strength, strain, tear strength and burst strength were evaluated. The density of handsheet was slightly decreased by polyelectrolyte multilayering. Formation did not show a significant change, but all mechanical properties were increased by polyelectrolyte multilayering. Remarkable improvement in tensile and tear strengths was obtained when pulp fibers were treated with cationic starch and poly styrene 4-sulfonate. Irrespectively of final ionicity of pulp fiber, tensile index, strain and tear strength of paper could be improved simultaneously by polyelectrolyte multilayering.

• Transactions of the Korean Society of Pressure Vessels and Piping
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• v.5 no.2
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• pp.20-26
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• 2009

The dominant incidents category for onshore and offshore natural gas transmission pipelines in the world is associated with outside forces. Incidents in the outside forces category embrace acts of nature, which typically cause widespread structural damage, as well as act of man, whose effects tend to cause dents and/or gouges localized at point of contact that are referred to as mechanical damage. Therefore, these damage types must be better addressed to avoid unnecessary and costly repairs and the possibility of catastrophic events. First of all, the characterizing features of mechanical damage in gas pipelines were evaluated by using of excavator or boring machine. There is no reliable method for evaluating the safe operating pressure of pipeline affected by mechanical damage. It is especially important to evaluate the remaining strength of damaged pipelines due to outside force. Therefore, the full scale burst tests were conducted to evaluate the remaining strength of pipe with mechanical damage that combines a dent and a gouge. This paper is supposed to provide information that will assist in developing a criterion to assess serviceability in pipelines with mechanical damage.

• Fashion & Textile Research Journal
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• v.13 no.6
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• pp.990-995
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• 2011

This study analyzed the effects of the knit stitch type, fiber composition, and yarn thickness on the mechanical properties of knitted fabric. The results were as follows: The course density was the highest in the case of the rib stitch. On the other hand, the wale density was the highest in the case of the float stitch. The thickness was the highest in the case of the rib stitch. The same results were obtained even for different fiber compositions and yarn thicknesses considered in this study. The burst strength of wool knit fabric was higher than that of A/W knit fabric. The stiffness was the lowest in the case of the plain stitch. The same results were obtained even for different fiber compositions and yarn thicknesses. The pilling properties were excellent for all knit stitches, fiber composition, and yarn thicknesses as pilling degree : 5. The air permeability decreased in the following order : rib > plain > float stitch. The same results were obtained even for different fiber compositions and yarn thicknesses. The heat retention rate decreased in the following order : rib > float > plain stitch. The same results were obtained even for different fiber compositions and yarn thicknesses.

• The Bulletin of The Korean Astronomical Society
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• v.35 no.2
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• pp.45.2-45.2
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• 2010

We analyzed onset times of the largest six solar proton events during 1997-2006 of solar cycle 23, as observed at 1AU by two satellites of GOES/SEM (Geostationary Operational Environmental Satellites/the Space Environment Monitor) and SOHO/ERNE (Solar and Heliospheric Observatory/the Energetic and Relativistic Nuclei and Electron). We adopted the time shifted method suggested by Leon Kocharov and determined the path length by Sam Krucker's fitting method. We found some problems of those methods and tried to improve those. In this presentation, we will give details of the energy spectra of the 6 SPE events from the ERNE/HED, and onset time comparison among the SPE, flare, type II burst, and CME.

• Journal of Microbiology and Biotechnology
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• v.2 no.3
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• pp.209-214
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• 1992

Serratia marcescens co-cultured with various phytopathogenic fungi, including Rhizopus stolonifer, Helminthosporium allii, Pyricularia oryzae, Fusarium oxysporium and Collectothricom cassiicola, in an LB- agar medium containing 1.5% swollen chitin, significantly inhibitied fungal growth. Fungal hyphae grew rapidly outward from the culture dish center, but the hyphal extensions of the pathogenic fungi were significantly inhibited in a perimetric contact area with S. marcescens. This was especially evident in pathogenic fungi which have a high chitin content in their cell walls. The extracellular chitinase activities of S. marcescens were increased seven fold by the addition of 1.5% swollen chitin to the LB-broth, compared to chitinase activities in a culture medium without chitin. The type of induction was dependent on the various forms of chitin used. When the culture supernatant of S. marcescens or the chitinases of Streptomyces griceus purchased from Sigma Chemical Co., were incubated with the mycelium of F. oxysporium, the mycelium gradually burst as cultivation time progressed and completely lysed after incubation for 2 days. On the other hand, E. coli extract did not hydrolyze the F. oxysporium mycelium at all. These data showed that the chitinolytic activities of S. marcescens play important roles in the biochemical control of phytopathogenic fungi.