• The Plant Pathology Journal
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• v.24 no.2
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• pp.159-163
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• 2008

Accurate identification of pine wood nematode, Bursaphelenchus xylophilus is a prerequisite to diagnose the pine wilt disease. However, a fungivorous nematode, B. mucronatus is highly similar to B. xylophilus and it is difficult to differentiate these two species by morphological features. A molecular diagnosis method, ITSRFLP was applied for the identification of B. xylophilus and B. mucronatus from Korea. Genomic DNA was extracted from a single individual nematode and ITS DNA was amplified by PCR. The size of PCR product was approximately 900bp and the sequence data were obtained after cloning. Amplified ITS was digested by 5 different restriction enzymes (Rsa I, Hae III, Msp I, Hinf I, and Alu I) and provided a discriminatory profile for B. xylophilus and B. mucronatus. Besides, B. mucro- natus was determined to have 2 different genotypes, East Asian type and European type also clearly separated by Rsa I and Hae III digestion. European type of B. mucronatus is recently collected from Pinus koraiensis and has not been reported before. ITS sequnce data were analyzed by Restriction Mapper program and the result supported ITS-RFLP pattern. These data indicated that PCRRFLP method is an accurate and simple way for identification of Bursaphelenchus species.

• The Journal of the Convergence on Culture Technology
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• v.7 no.2
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• pp.443-451
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• 2021

This study compared and analyzed the thermal characteristics based on the ratio of moisture content of infected and non-infected Bursaphelenchus Xylophilus by heat of combustion experiment. The experiment results are as follows. The analysisresult of the ratio of water content shows that 19.92% on average for infected Bursaphelenchus Xylophilus and 26.27% for non-infected which decreases water content as the size of conduction, convection, and radiant heat increases in case of fire. As the vaporization of the moisture content accelerates, the average moisture content is reached, and the result is contrary to the thermal characteristics of the wood that the moisture content is not ignited by a heat source when the moisture content is under 15%. The combustion heat analysis result showed that infected and non-infected Bursaphelenchus Xylophilus had no significant difference in combustion heat at about 3%. However, it shows that combustion heat is higher than other species. In conclusion, decreasing in moisture content based on the increasing conduction, convection, and radiant heat is one of the direct causes of ignition, and the lower the moisture content, the faster the fire spreads.

• Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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• 2007.04a
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• pp.283-287
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• 2007

Pine Wilt Disease (Bursaphelenchus Xylophilus) has been attacked since 1988, then it becomes very serious problem of the all over the country. Government has been invested a lot of money to prevent but it is hard to survey the damaged area and ineffectively control the process of prevention. Therefore, this study is focused on development of Pine Wilt Disease (Bursaphelenchus Xylophilus) Prevention System using GIS and GPS through constructiong database of digital map, satellite imagery and attribute data and development component for desktop PC, internet and mobile system to realtime data transmission between project manager and field worker. Especially, we developed the mobile system that can transmit field conditions in realtime using GPS, GIS and CCD camera, the telecommunication control server that transmit received field condition data to web connecting module and system manager, the web system for end user to notify prevention details, the control system lot manager to recognize filed conditions and to control field workers and the wireless telecommunication module to connect in realtime between field and control center.

• Animal cells and systems
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• v.14 no.1
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• pp.31-36
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• 2010

The pinewood nematode Bursaphelenchus xylophilus causes pine wilt disease and is a serious economic concern for the forest industry of South Korea. To achieve effective control with limited resources, it is necessary to clarify the transmission routes and mechanisms of dispersal of this organism. Highly polymorphic and easy-to-use molecular markers can be used for investigating this aspect. In this study, we evaluated the usefulness of amplified fragment length polymorphisms (AFLPs) for investigating the genetic variations of B. xylophilus and related individuals from China, Japan, and South Korea. The AFLP patterns obtained in our study were similar to the microsatellite patterns reported in a previous study; our AFLP patterns indicated high genetic variability and cryptic genetic structure, but did not indicate any peculiar geographic structure. Moreover, the genetic distances between individuals suggested that the Korean population was affected to a greater extent by the Chinese population than the Japanese population. Further, the gene flow among the related species appeared to be limited; however, there may be also the possibility of genetic introgression among species. These results confirm the usefulness of AFLPs for understanding the epidemiology of pine wilt disease, thereby contributing to the effective control of this disease.

• Research in Plant Disease
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• v.20 no.3
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• pp.227-233
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• 2014

The mixture (MX) of acetic acid (AA) and lactic acid (LA) was examined for its effectiveness in the control of the pine wood nematode Bursaphelenchus xylophilus contaminated in cymbidium culture medium. Nematode mortality in vitro was nearly 100% in AA and MX at the concentrations of 5.0-1.0% (pH 2.6 - 4.2) and in LA only at 5.0% (pH 3.5), but lowered at concentrations of 0.5-0.1% (pH 5.1-6.9) more significantly in LA than AA and MX. MX of most concentrations caused higher nematode mortality than the average response to AA and LA. All treatments of MX (0.5% and 0.25%), fosthiazate (standard and double concentrations) and culture dilution of Paenibacillus polymyxa GBR-1 ($10^7$colony-forming units/ml) reduced significantly the nematode populations in the cymbidium culture medium, compared to non-treatment control, with no significant difference among the treatments. No phytotoxicity occurred in all treatments. pH of the medium with the time after treatment and growths of 2-year-old cymbidium were not significantly different among treatments. Considering the safety and price of the organic acids, use of MX in the processes for culturing cymbidium may be a practically reliable and eco-friendly way in the control of the quarantine nematode in cymbidium.

• Proceedings of the KSRS Conference
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• 2001.03a
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• pp.62-69
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• 2001

본 연구에서는 해상도가 상이한 시기별 위성영상과 GIS를 이용하여 경남 통영시 한산면 추봉도 지역의 소나무재선충(Bursaphelenchus Xylophilus) 피해지역을 탐지하고 다양한 영상처리를 통하여 이를 효율적으로 추출 할 수 있는 기법을 선정하였다. 연구결과 피해지역의 공간적 범위를 추출하기 위해서는 감독분류의 MHC(Mahalanobis Distance Classification)가 유용하였고 벌채 후의 토지피복 분류로 인한 피해지역 추출을 위해서는 MLC(Maximum Likelihood Classification)가 최적한 기법으로 나타났다. 아울러 이에 관련된 GIS를 구축하여 공간정보를 추출함으로써 피해지역의 공간적 분포특성을 규명하였는데 고도 약 120-160m, 경사 21$^{\circ}$-40$^{\circ}$ 그리고 서쪽 방향 사면에서 소나무재선충이 가장 활발하게 활동하였음이 밝혀졌다.

• The Plant Pathology Journal
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• v.29 no.1
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• pp.116-122
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• 2013

To evaluate the mechanisms of pathogenicity and nonpathogenicity of Bursaphelenchus xylophilus and B. mucronatus isolated in South Korea, we used 4-year-old P. thunbergii seedlings and 20-cm long one-year-old stem cuttings of 5-year-old Pinus thunbergii, and studied distributions and multiplications of pine wood nematodes after inoculation. The distributions of B. xylophilus in the 20-cm pine stem cuttings were not significantly different from that of B. mucronatus. Conversely, the proliferation rate of B. xylophilus on mycelial mats of Botrytis cinerea was significantly different from that of B. mucronatus. The study using 4-year-old P. thunbergii seedlings also showed that B. mucronatus can migrate to distal portions of the pine seedlings the same as B. xylophilus, but the populations of B. xylophilus remaining in the pine seedlings were relatively larger than those of B. mucronatus. Therefore, we concluded that the pathogenicity of B. xylophilus could be strongly influenced by its ability to multiply.

• Journal of Korean Society of Forest Science
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• v.78 no.2
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• pp.248-253
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• 1989

• Journal of the Korean Association of Geographic Information Studies
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• v.4 no.4
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• pp.72-78
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• 2001

In this study, high spatial resolution of IKONOS 1m image and Red(0.63~0.69) band, NIR(0.76~0.90) band in 4m image, which are the same wavelength range as Landsat TM band 3, 4, were used for extraction of the front areas of B. Xylophilus in Geuje island where is located in southern part of Korea. Moreover, since they have higher spatial resolutions than Landsat TM, they have been used for lots of studies in the field of forest and vegetation. In the results, it was validated by GPS field survey, spectral histogram analysis of IKONOS NIR band was significant available method for extracting the front areas of B. Xylophilus. In this study, 15 points were verified as real damaged trees of 22 sample points extracted from GPS field survey. This study was not only extracted the damaged trees by B. Xylophilus but also suggested the possibility of using IKONOS images for the study on the forest damages by any disease and insect pests.

• The Plant Pathology Journal
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• v.24 no.2
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• pp.178-182
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• 2008

Five essential oils and 15 herbal extracts were evaluated to control Bursaphelenchus xylophillus in laboratory. The essential oils from clove plant (Syzygium aromaticum), mustard (Brassica integrefolia), thyme (Thymus vulgaris), and Pelargonium inquinans were found to be highly promising and gave excellent control of the nematodes at all the time of exposure. Among them, the least one gave 91.3% mean mortality rate at 24 hours of exposure time, which is highly significant from the control. While in the second study, most of the methanol (Desmodium caudatum, Paulownia coreana, Auckulandia lappa, Sophota flavescens, Aloe sp., Rheum palmatum, Zingiber officinale, Magnolia officinalis, and Eugenia caryophyllata), hexane (Torreya nucifera, Pharbitis nil, Prunus mume, Melia azedarach, and Xanthium strumarium), and hot water (Cinnamomum cassia) herbal extracts killed the nematodes, but in varying degrees compared to the control. Only one extract was found to be promising viz Magnolia officinalis which found to be statistically different from the control and gave mean mortality of 72, 82.3, and 85.3 % for 24, 48, and 72 hours exposure, respectively. Further screening was conducted for M. officinalis with concentrations of 1,000, 100, and 10 ppm against the same species of nematode with the same time of exposure. However, it gave an excellent result for 1,000 ppm for all time of exposure, whereas for the 100 and 10 ppm it gave mean mortality of 39.5 and 25.8% for the time 72hrs, respectively that were statistically different from the control.