• Journal of the Korean Society of Tobacco Science
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• v.12 no.2
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• pp.77-83
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• 1990

This study was conducted to develop the method of hydrocarbon analysis and investigate hydrocarbon contents in flue-cured and burley tobacco leaves harvested in Korea and U.S.A. Tobacco leaf was extract with hexane in soxhlet apparatus. Hydrocarbon was fractionated from hexane extract by silica gel column chromatography, and then separated and indentified by GC, GC/MS using SE-54 fused silica capillary column. The developed method was feasible to analyze neophytadiene, normal and branched hydrocarbons from Clo to Cn. The result of recovery test was decade 99%, eicosane 100%, triacontane 102%. The major hydrocarbon of tobacco leaves were neophytadiene, nC3l, iC31, nC33, aC33, aC32, aC30 and nC29. The amount of total hydrocarbon in burley and flue-cured tobacco leaves harvested in U.S.A. and Korea were 4591, 2931, 2929 and 3015$\mu\textrm{g}$/g, respectively.

• Journal of the Korean Society of Tobacco Science
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• v.8 no.2
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• pp.3-8
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• 1986

This study was carried out to investigate the effect of different soil moisture contents on the growth and chemical constituents of burley tobacco and on the protein pattern in tobacco leaf. Height, stem diameter, and largest leaf length of tobacco droughted from 45 to 60 days after transplanting was not recovered by rewatered amount of water supply from 60 to 75 days after transplanting, but leaf width enlarged. Dry weight per unit leaf area and total nitrogen content showed high values in low soil moisture, but total alkaloid contents were not different according to soil moisture contents. Soil moisture content didn't effect on the protein pattern of middle and upper leaves, but lower leaves showed the mild color and fewer numbers of the protein bands than those of midd1e and upper leaves.

• Journal of the Korean Society of Tobacco Science
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• v.17 no.1
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• pp.15-19
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• 1995

This study was made of the effects from exposure to air temperature and amount of solar radiation during the wilting period on the sunburn of burley tobacco. Tobacco stalks were cult with one hour interval from 8:00 to 16:00, and were left on the field to expose to weather, and tobacco single leaf placed under 4 temperatures regimes in indoor. Sun-burn of the leaves was occurred when the leaves were exposed to temperature higher than 33$^{\circ}C$, and to solar radiation more than 800w/$m^2$ between 11:00-15:00 in a day during the curing season. The leaf temperature was increased until 52$^{\circ}C$ under the weather condition. The tobacco leaves were also burned without solar radiation when the leaves were exposed to high temperature until 5$0^{\circ}C$ of leaf temperature. Sun-bum was increased at upper leaves and at higher moisture content of leaf.

• Journal of the Korean Society of Tobacco Science
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• v.1 no.2
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• pp.93-102
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• 1979

Chemical compositions of Flue - cured and Burley tobacco leaf and smoke were analyzed to find out simple correlation between compositions. Based on the simple correlation ( $r$ ) data, four regression equations were obtained for dry condensate and nicotine in smoke as follows, $Y_{FT}=\;0.816+4.638X_1+0.346X_2$......................................(1) $Y_{BT}=\;0.235+0.884X_3-0.088X_4$......................................(2) $Y_{FN}=\;0.015+0.090X_5+0.476X_1$......................................(3) $Y_{BN}=-0.026+0.101X_3-0.006X_4$.........................................(4) where, $Y_{FT}$ : dry condensate (mg/cig.) in cigarette smoke of Flue-cured $Y_{BT}$ : dry condensate (mg /cig. ) in cigarette smoke of Burley $Y_{FN}$ : nicotine contents (mg/cig.)in cigarette smoke of Flue-cured $Y_{BN}$ : nicotine contorts (mg/cig. ) in cigarette smoke of Burley $X_1$ : total alkaloid contents (%) of Flue-cured $X_2$ : curde ash contents (%)of Flue -cured $X_3$ : alcohol -benzene extract contents (%) of Burley $X_4$ : curde ash contents (%) of Burley $X_5$ : hexane extract content (%) of Flue -cured Standard deviation for regressions equations were obtained respectively. equation(1),$\pm$3.16 : equation (2),$\pm$1.94 : equation (3),$\pm$0.35 , equation (4) $\pm$ 0.33. Regression equations between dry condensate and nicotine contorts in cigarette smoke were also obtained in the forms of equation(5) and(6) $Y_{FN}$= 0.274 $Y_{FT}$- 3.259............................(5) $Y_{BN}$= 0.249 $Y_{BT}$ - 2.093.............................(6) Standard deviations, $\pm$ 0.061 and $\pm$ 0.152, were obtained for regression equation(5) and (6) respectively.

• Journal of the Korean Society of Tobacco Science
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• v.17 no.2
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• pp.177-183
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• 1995

This study was conducted to develop the most precise NIR(near infrared spectrometric) calibration for rapid determination of chemical composition in ground samples of toasted burley tobacco using stepwise, stepup, principal component regression(PCR), partial least square(PLS) and modified partial least square(MPLS) calibration method. The number of wavelength(W) selected by stepup multiple linear regression using: second derivative spectra was as follows: total sugar(TS)-4 W, nicotine-9 W, total nitrogen(TN)-2 W, ash-8 W, total volatile base(TVB)-5 W, chlorine4 W, L of color-6 W, a of color-6 W and b of color-7 W. Comparing the calibration equations followed by each chemical components, the most precise calibration equation was MPLS for 75, a and b of color, PLS for nicotine, ash, TVB, chlorine and L of color and stepup for TN. The standard error of calibration(SEC) and standard error of performance(SEP) between result of near infrared analysis and standard laboratory analysis were 0.18, 0.40% for 75, 0.06, 0.08% for nicotine, 0.18, 0.16% for TN, 0.33, 0.46% for ash, 0.04, 0.03% for TVB, 0.08, 0.06% for chlorine, 0.54, 0.58 for L of color, 0.22, 0.22 for a of color and 0.27, 0.27 for b of color, respectively. The SEC and SEP of ash and TVB were within allowable error of standard laboratory analysis, nicotine, TN and chlorine were 1.2-2.0 times and 75 were 2.1-4.0 times larger than allowable error of standard laboratory analysis. The ratio of SEC and SEP to mean were 1.5, 1.6% for L of color, 3.7, 3.8% for a of color and 1.8, 1.8% for b of color, respectively. Key words : burley tobacco chemistry, near infrared spectroscopy.

• Journal of the Korean Society of Tobacco Science
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• v.12 no.2
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• pp.91-101
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• 1990

A field experiment was conducted to find out the effects of field productivity, variety and nitrogen rate on the yield, quality, chemical constituents and physical properties of burley cured leaf in three field with different productivity(Degree of field productivity: A ; high, B ; medium, C : low) during successive two years(1988~89). The yield and quality were remarkably lowered when nitrogen fertilizer being applied much in low productive field. As compared with Burley 21, KB101 showed high yield, particularly the yield of KB101 in low productive field was relatively high. The effect of nitrogen rate on the yield was somewhat different according to field productivity and production year. When the nitrogen fertilizer being applied above 22.5kg/10a, the added nitrogen had no effect on the yield. Total nitrogen content of cured leaf grown in low productive field was high while total alkaloid was low, therefore total alkaloid/total nitrogen ratio was remarkably low. The lightness, red and yellow color of cured leaf grown in low productive field was remarkably low. As compared with Burley 21, the contents of total alkaloid and total nitrogen and shatter resistance index of cured leaf was somewhat low, while the filling power, lightness, red and yellow color were slightly high. Total nitrogen content of cured leaf was increased remarkably by nitrogen addition, but total alkaloid was not increased though the nitrogen fertilizer being applied above 22.5kg/10a. The filling power and shatter resistance index of cured leaf grown in high nitrogen plot, and the lightness and yellow color were low while the red color was relatively high. It comes into question that the visual quality being increased as well as increment of yield and nitrogenous compounds by nitrogen addition in high productive field. In low productive field, it is considerable that nitrogen addition for high yield should be prohibited because it causes the decrement of yield and quality, on the contrary.

• Journal of the Korean Society of Tobacco Science
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• v.13 no.2
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• pp.66-70
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• 1991

A field experiment was conducted to study the effects of fertilizer application rate and number of harvested leaves per plant on selected agronomic, chemical and physical characteristics of burley tobacco(Nicotiana tabacum L. cv Burley 21) in 1988, 1989 and 1990 at Chonju Experiment Station, Korea Ginseng SE Tobacco Research Institute. 175.0 or 227.5 kilogram per 10a of compound fertilizer(N-P2O5-K2O= 10-10-20) were applied as main plot, and 2 or 4 of upper leaves was topped off with 0, 2 or 4 of the lower leaves removed as sub plot. Yield, total alkaloid and total nitrogen content of cured leaves were high in the higher level of compound fertilizer plot, and increasing the level of compound fertilizer had an adverse effect on physical characteristics. Reducing the number of harvested leaves had a negative effect on yield regardless of whether the leaf number was reduced by lower topping or removing the bottom leaves, but increased the rate of higher grades leaves. Differences in total alkaloid, total nitrogen and some physical properties among number of harvested leaf per plant were smaller than expected.

• Journal of the Korean Society of Tobacco Science
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• v.5 no.2
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• pp.25-32
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• 1983

To obtain basic information on the breeding of burley tobacco, classification of 41 varieties was carried out by using the cluster analysis of correlation coefficients and taxonomic distance based on twenty-one agromonic characters. Eight characters, such as days to flowering, length of flower axis, internode length, leaf length, yield, leaf angle to stem, vein angle to midrib and plant height, were useful in monothetic classification. Forty-one varieties were classified into four groups (I, II, III and IV) with weighted variable group method (WVGM ) and weighted jai. group method(WPGM), whereas the results classification of 33 varieties among them by WVGM were coincident with the results by WPGM. As for the characteristics of each group, group I related to late maturity, tall height and high yield, group II related to intermediate maturity, tall height and low yield, group 19 related to early maturity, intermediate height and low yield, and group W related to early maturity, short height and intermediate yield.

• Journal of the Korean Society of Tobacco Science
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• v.5 no.1
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• pp.25-33
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• 1983

This study was carried out to investigate the transplanting time, optimum fertilization and harvesting method, chemical contents and physical properties of burley tobacco in paddy field. The results obtained as follows. 1. It is recommendable to transplant after March. 29 to escape the frost injury. By reducing compound fertilizer and whole plant stalk curing, advanced the. last priming date by 5-8 days. 2. Total alkaloid, total nitrogen and phosphorus contents of cured leaf was comparable to those of upland-produce(1 tobacco, but potassium and chloride contents some what high. 3. Filling power and combustibility was also comparable to upland, and filling power was increased by reducing fertilizer and whole plant stalk curing. 4. In the 20o reducing fertilizer-5 Primings volt. the yield was some what decreased, but visual quality and value per 10a were high. 5. The total raw income of tobacco and rice cropping was increased 124~170% than that of rice cropping.

• Journal of Biosystems Engineering
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• v.22 no.1
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• pp.30-40
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• 1997

A computer vision system was built to automatically grade the leaf tobacco. A color image processing algorithm was developed to extract shape, color and texture features. An improved back propagation algorithm in an artificial neural network was applied to grade the Burley type dried leaf tobacco. The success rate of grading in three-grade classification(1, 3, 5) was higher than the rate of grading in six-grade classification(1, 2, 3, 4, 5, off), on the average success rate of both the twenty-five local pixel-set and the sixteen local pixel-set. And, the average grading success rate using both shape and color features was higher than the rate using shape, color and texture features. Thus, the texture feature obtained by the spatial gray level dependence method was found not to be important in grading leaf tobacco. Grading according to the shape, color and texture features obtained by machine vision system seemed to be inadequate for replacing manual grading of Burely type dried leaf tobacco.