• Journal of the Korean Society of Tobacco Science
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• v.25 no.1
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• pp.27-33
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• 2003

This study was conducted to determine the changes of nitrogen compounds in lamina and midrib during the curing process with the different curing methods, such as priming and stalk-cut curing. After KB 108 burley tobacco was cultivated by the different fetilization levels such as standard and 20% higher, only the tips and leaf were harvested. Though the contents of total alkaloid and total nitrogen were similar in lamina, midrib showed a very low contents of those components by the different curing method and fertilization levels. The content of nitrate-nitrogen in lamina increased during a middle of curing process, and then these compound was decreased during an end of curing process by stalk-cut curing method. Nitrate-nitrogen contents in the lamina by the priming curing showed a high level caused by rapid drying process during an end of curing process. That component in midrib was 5-6 times higher than that of lamina. The contents of ammonia-nitrogen in the lamina and midrib were increased during a curing process. Though those amount in tips showed a similar by a different fetilization and curing method, midrib of stalk-cut curing showed a slow increasing during a curing process.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.3
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• pp.235-238
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• 1989

This study was carried out to investigate the effect of environmental conditions of air temperature and relative humidity, varieties, and water content of leaves at harvesting time on the occurrence of barn rot during curing of burley tobacco. The curing environmental condition was combined with 4 air temperatures ranging from 25$^{\circ}C$ to 40$^{\circ}C$ and 3 different relative humidities. The harvested leaves with 3 different water contents were cured during the rainy season in curing barn. Barn rot occurred the most at 30$^{\circ}C$, and reduced at 25$^{\circ}C$ and remarkably decreased at above 35$^{\circ}C$. But no barn rot was observed at 40$^{\circ}C$. In influence of relative humidity, the percentage of rotten leaves was highest at 100% RH and remarkably reduced at lower RH. Among two varieties, KB 101 was rotted smaller than Burley 21 under the all temperature and relative humidity conditions, however those considerably showed no difference. The rate of disease development increased in the lower leaves more than in the upper leaves. In the water content of leaves at harvesting time, 29.5% of the rotten leaves was observed at W.S.D. (water saturation deficit) 10.3%, but no barn rot was found at W.S.D. 6.4%.

• Journal of the Korean Society of Tobacco Science
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• v.16 no.2
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• pp.152-162
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• 1994

This study was designed to establish an analytical method for the properties of leaf tobacco smoke. Lyophilized TPM from leaf tobacco smoke was extracted with MeOH, dried under reduced pressure, and trimethyl-silylated(TMS). Gas chromatography of the material using SPB-5 column showed 120 quantifiable peaks. Among those, 26 compounds including a hydrocarbons, Neophytadiene. and Levulinic acid could be identified through GC-MS. Smoke properties of 5 manufacturing grades and 2 oriental cultivars of domestic and imported leaf tobacco including AB3O-1 were analyzed. For flue-cured tobacco, content of the compounds in the smoke was generally higher in American leaf tobacco except for glycerol compounds. For burley tobacco, domestic leaves were found to have much higher amount of smoke compound than imported leaves. Among oriental tobacco, Izmir contained slightly higher amount of smoke compounds than Basma. Key words : GC - profile. TPM. TMS. Leaf tobacco.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.2
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• pp.146-150
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• 2011

A large amount of nitrogenous compounds are found in burley tobacco and are responsible for peculiar aroma during smoking. Excess in nitrogen or its compounds such as total nitrogen and proteins in burley tobacco gives a strong pungency and make its taste bad. The present study has focused on improving quality of Burley tobacco by regulating topping and harvesting time of leaf to reduce nitrogen compounds in Burley tobacco. In addition, the early-stage of flower topping had lower level of nitrogenous compound, such as total nitrogen and protein nitrogen, and total tobacco-specific nitrosamine (TSNA) contents compared to the button-stage and full flower-stage topping. Also, the contents of nicotine got significantly decreased while the ether extract was increased as the period of topping got delayed. Regarding the brightness among the colors of cured leaves, the color at the full flower-stage topping was the brightest, and there was no particular difference in red and yellow colors. The harvesting amount was decreased as the period of topping got delayed and there was no significant difference between the topping treatments in terms of the exterior quality. As the harvesting time delayed, the total nitrogen, protein nitrogen, nitrate nitrogen, and ammonium nitrogen were decreased while the nicotine contents got increased. Also, the total TSNA contents were decreased as the harvesting time delayed. However, ether extract was increased. Among the colors of cured leaves, the brightness and degree of yellow color were significantly increased as the harvesting time delayed, and there was no particular difference in the degree of red color. The harvesting amount was decreased along with the delayed harvesting time; however, the harvest of the matured and mellow leaves was higher in terms of price, compared to that of the immature leaves. For the reduction of nitrogenous compound and TSNA contents in Burley tobacco, the topping was examined to be more effective in terms of improvement in chemical contents and quality regardless of the decreased harvesting amount.

• Journal of the Korean Society of Tobacco Science
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• v.30 no.2
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• pp.94-102
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• 2008

In this paper, we describe our study on the characterization of tobacco leaves by their pyrolysis patterns. Two kinds of tobacco leaves were pyrolyzed and analyzed by Double-Shot Pyrolysis-Gas Chromatography/Mass Spectroscopy (Py-GC/MS) methods. Three grades of Korean flue-cured tobacco leafsuch as B1O, AB3O, CD3L and burley tobacco leaves such as B1T, AB3T, CD3W were pyrolyzed with six discrete but stepwise heating temperature ranges, those are from 100$^{\circ}C$ to 150$^{\circ}C$, 150$^{\circ}C$ to 200$^{\circ}C$, 200$^{\circ}C$ to 250$^{\circ}C$, 250$^{\circ}C$ to 300$^{\circ}C$, 300$^{\circ}C$ to 350$^{\circ}C$ and finally from 350$^{\circ}C$ to 400$^{\circ}C$. Using the resultant 52 pyrolytic components identified in the programs as components, principal component analysis (PCA) showed statistical classification between flue-cured and burley tobacco lamina. Among six pyrolysis temperature ranges, the best discrimination was achieved at the temperature range from 250$^{\circ}C$ to 300$^{\circ}C$ and from 300$^{\circ}C$ to 350$^{\circ}C$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.5
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• pp.649-654
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• 1995

This study was conducted to investigate the utility and agronomic characteristics and use of cytoplasmic. male-sterile (CMS) haploid plants derived from interspecific cross between (male-sterile NC82$\times$burley21) F$_1$ plant (Nicotiana tabacum L.) and Nicotiana africana. Abundant seeds of high germinability were obtained when Nicotiana tabacum (cytoplasmic male-sterile F$_1$ plants) is pollinated by Nicotiana africana. Most of seedlings died at the cotyledonary stage. The remaining seedlings are viable F$_1$ hybrids or maternal haploids that can be easily distinguished. Number of interspecific Fl hybrids and matermal haploids per capsule obtained from the interspecific cross between cytoplasmic male-sterile tobacco F$_1$ plants and N.africana yielded 2.2 and 0.5 plants, respectively. Out of 149 CMS haploid plants obtained from interspecific cross, 102 plants showed green type while the others were yellow type for leaf and stem. This results agreed with the genetic ratios expected among haploid plants from the F$_1$ hybrids heterozygous for two recessive genes of yellow color of burley tobacco plant. Out of 83 CMS haploid plants inoculated with TMV, 48 plants showed resistant, while the others was susceptible. It agreed with the expected genetic ratios for a single dominant gene of TMV resistance. CMS haploid plant will be useful as a source material for breeding of CMS doubled haploid lines

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.1
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• pp.139-143
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• 1983

Nicotiana Tabacum L., cv. NC2326, Burley2l and Sohyang seeds were coated with different five materials - zeolite, serpentine, coal briquet ash, serpentine+zeolite and coal briquet ash+zeolite - to investigate the effects of coating on germination. The coating materials had different characteristics and gave varied effects on germination. Among three tobacco varieties, coated seeds of Burley2l showed the most sensitive responses in germination rate of decreasing and delaying at low temperature on seedbed and laboratory tests.

• Journal of the Korean Society of Tobacco Science
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• v.3 no.2
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• pp.129-130
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• 1981

The potato tuberworm, Phthorimaea operculella, is an occasional pest to tobacco plants in southern regions of Korea. In order to screen effective pesticides against the worm in tobacco leaves, pot and field experiments were carried out in 1981. In the pot experiment with a tobacco variety, Hicks, several effective organic phosphates (Dimecron- LQ 50, Dursban-WP 25, Ekalux-EC 25. Elsan-EC 47.5, and EPN-EC 45), out of 9 Pesticides tested, were solecte4 from their high mortality of more than 90%. Among these Pesticides Ekalux- EC 25 and Elsan- EC 47.5 were recommended to tobacco growers from their excellent efficacy in the field experiment with a tobacco variety, Burley 21.

• Journal of the Korean Society of Tobacco Science
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• v.15 no.1
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• pp.90-97
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• 1993

1. Average moisture content of tobacco in BIB silo was about 19: 1% until 4hours conditioned time, and then moisture variation of tobacco after 2hr conditioned was very small. 2. Application of mathematical model for ordering system. 1) The constant K in the exponential equation varies inversely with both relative humidity and equilibrium moisture. 2) Time needed to order blending tobacco leaves with standard moisture from bulking and blending silo was 4 hours. 3) Reconstituted tobacco sheet had higher moisture absorption ratio than Oriental and Burley tobacco. 4) For minimize of conditioning time in BIB silo, the values of K and Mo given in this study can be used in equation(1) to calculate moisture absorption ratio and optimum conditioning time. 3. Average moisture content and water activity of conditioned tobacco for 4 hours in BIB silo was about 20% and 0.65. In this condition. microbial life will inhibite 4. Physical properties of conditioned tobacco in bulking and blending silo for 4hours was virtually no change.

• Journal of the Korean Society of Tobacco Science
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• v.2 no.1
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• pp.1-7
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• 1980

Gel filtration column chromatography (Sephadex G-75), dialysis an d Brushite column chromatography were carried out to separate the brown pigmented macromolecule from water extracts of the cured leaf tobaccos. The two distinct macromolecules having different molecular weight were separated by the Sephadex column chromatography. Brushite also separated two different species of macromolecules which might have different electronic structures. According to the enzymatic degradation of protein in Burley and Hicks, chymotrypsin showed the best degradation ratio, ie., 16-30% in Burley and 38-57% in Hicks. Similar effect was observed with pepsin. However, very low effect of degradation was revealed with trypsin. The sample treated with the proteolytic enzymes revealed the disappearance of the first peak and the slight decrease of the 2nd peak height in the separation profile of Sephadex. After dialysis, the brown pigmented macromolecule was pyrolyzed at $300^{\circ}C$ and the strongly fluorescent components not identified before pyrolysis were detected with TLC separation. Absorption spectrum of these fluorescent compounds was monitored in benzene and the absorption maxima at 265nm and 275 nm were obtained. Considering absorption maxima and shape of the spectrum, those fluorescent compounds seem to be PAH derivatives.