• 한국환경복원기술학회지
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• 제9권6호
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• pp.86-94
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• 2006

This study was to analyze vegetation and soil characteristic, and ordination of Bupleurum euphorbioides community located in Mt. Seorak area, Mt. Sobaek area, and Mt. South Deogyu area. Bupleurum euphorbioides community was classified into Carex lanceolata subcommunity and Chrysanthemum zawadskii var. alpinum subcommunity, and Chrysanthemum zawadskii var. alpinum subcommunity was classified into Calamagrostis arundinacea and Carex breviculmis group. In the study sites, soil organic matter, nitrogen, available phosphorous, changeable potassium, changeable calcium, changeable magnesium concentration, cation exchange capacity, and soil pH were ranged from 9.0~15.2%, 0.32~0.47%, 3.0~8.6ppm, 0.3~0.9me/100g, 0.5~1.5me/100g, 0.2~0.6me/100g, 11.4~16.1me/100g, and 4.3~4.6, respectively. And, soil texture was sandy loam or loam. The Carex breviculmis group of Chrysanthemum zawadskii var. alpinum subcommunity was found in area of high elevation and had high exchangeable K, Ca, and Mg concentration. The Calamagrostis arundinacea group of Chrysanthemum zawadskii var.alpinum subcommunity was found in area of low elevation and had lower exchangeable K, Ca, and Mg concentration than in the other subcommunity. The Carex lanceolata subcommunity was found in area of medium elevation and had medium exchangeable K, Ca, and Mg concentration. The Carex lanceolata subcommunity was located in a path of top the mountain peak, and has been destroyed. We need any action to protect the subcommunity.

• 식물분류학회지
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• 제38권1호
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• pp.43-50
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• 2008

환경부 멸종위기종인 섬시호(Bupleurum latissimum)를 현지 내에서 보다 효과적으로 보존할 수 있도록 종합적인 종생물학적 정보를 수집하고자, 섬시호의 수분기작 및 수분매개자의 특성을 조사하였다. 섬시호는 5월 말에 개화하며, 꽃은 개화된 상태로 3일간 지속되었다. 꽃은 웅예선숙으로 수술이 성숙한 개화 1-2일째에 암술은 작은 돌기 형태를 이루었다. 꽃이 지기 시작하는 3일째부터 화주가 성숙하여 길게 나오고, 꽃잎은 노란색에서 붉은색으로 변하며 탈락하였다. 섬시호를 방문하는 곤충은 총 5목 11과 19종이었으며, 그 중 가장 효율적이며 주된 수분매개자는 호리꽃등에(Episyrphus balteatus)와 흰줄꼬마꽃벌(Lasioglossum occidens)이다.

• 한국응용과학기술학회지
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• 제41권1호
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• pp.149-157
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• 2024

시호는 약용작물의 하나로 해열, 진정, 간 장애 억제, 항염, 항암 등 우수한 약리 활성을 지니고 있다. 현재 식품 또는 약용으로 사용되고 있지만 화장품으로서의 사용은 미비하여 화장품 소재로서의 적합성 및 이용 가치를 검토하였다. 시호 추출물을 이용하여 폴리페놀, 플라보노이드, 항산화, 항염 실험을 진행하였다. 폴리페놀 함량은 14.71±0.16 mg·TAE/g, 플라보노이드 함량은 1.42±0.05 mg·QE/g를 나타내었다. 항산화 활성은 농도 의존적으로 우수한 활성을 보였으며, NO 발현 억제능은 1,000 ㎍/mL에서 61.21%의 저해율을 나타내어 항염 활성이 뛰어남을 확인하였다. 본 연구를 통해 시호 추출물의 항산화, 항염 효능이 뛰어나기에 화장품의 천연 소재로서 충분한 가치가 있다고 본다.

• Preventive Nutrition and Food Science
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• 제5권3호
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• pp.174-176
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• 2000

Seventeen wild vegetables consumed commonly in Korea were tested for inhibitory activities against alpha-glucosidase, followed by Bupleurum longeradiatum and Angelica decursiva. The hexane-soluble fractions of Hosta longipes, Ainsliaea acerifolia, Pedicularis resupinata, Bupleurum longeradiatum, and Angelica decursiva all at the concentration of 5 mg/ml, inhibited enzyme activity by greater than 50%, and the ethylacetate-soluble fractions of Hosta longipes, and Codonopsis lanceolata, and Bupleurum longeradiatum had relatively strong inhibitory activity against the enzyme. These results suggest that some edible plants merit further evaluation for clinical usefulness as anti-diabetic drugs.

• Natural Product Sciences
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• 제21권2호
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• pp.71-75
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• 2015

In this study, phytochemical investigation on the aerial parts of Bupleurum falcatum resulted in the isolation of fourteen compounds including three quinic acid derivatives (1 - 3), five flavonoids (4 - 8), three monoterpene glycosides (9 - 11), and three saikosaponins (12 - 14). Compound 1 was first isolated from nature and unambiguously determined to be 3-O-feruloyl 5-O-caffeoylquinic acid on the basis of the extensive spectroscopic evidence. Biological testing revealed that saikosaponin A (12) and saikosaponin D (13) showed moderate antiproliferative effects on HL-60 and HepG2 cancer cell lines.

• 한국약용작물학회지
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• 제14권6호
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• pp.342-346
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• 2006

Anatomical characters of the Bupleurum latissimum Nakai, an endemic species of Korea, were investigated to confirm its phylogenetic relationships. Compare to other species with anatomical characters, B. latissimum is very similar with B, euphorbioides and B, longeradiatum in point of lacking of pith in the stem, shape of involucres, number of vascular bundles in radical leaf and cauline leaf, and lacking stomata in adaxial leaf surface. The other hand, protruded pollen aperture character appears in B. latissimum and B. euphorbioides. On the based of anatomical characters, therefore, B. latissimum has closest relationships with B. euphorbioides and B. longeradiatum. It also needs molecular study including Asian species in order to confirm phylogenetic position and speciation process apparently.

• 생약학회지
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• 제21권3호
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• pp.205-209
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• 1990

The study for the quantitative analysis of saikosaponin of Bupleurum falcatum root produced by tissue culture was attempted. The optimal concentration of 2,4-D was 0.1 mg/l for inducing the callus. The induction and growth of the adventitious root from the callus was obtained in the suspension culture containing MS basal medium supplemented with no plant growth regulators. The results of the quantitative analysis of saikosaponins by high performance liquid chromatography (HPLC) showed that the content of saikosaponin a and c was high in the one-year-old root from somatic embryos and that of saikosaponin d was remarkably high in three-months-old adventitious root from callus.

• Archives of Pharmacal Research
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• 제25권5호
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• pp.640-642
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• 2002

The ethyl acetate fraction of Bupleurum longiradiatum was found to have an inhibitory effect on the tube-like formation of human umbilical venous endothelial (HUVE) cells. The active compounds, isolated from the fraction, were identified as acetylbupleurotoxin (P1) and bupleurotoxin (P2). The compounds P1 and P2 completely inhibited the tube-like formation of HUVE cells at 30 ${\mu}g$/ml, below the cytotoxic concentration. But, they did not exhibit antitumor activity on BDF1 mice bearing Lewis lung carcinoma cells despite their antiangiogenic activity.

• Journal of Plant Biology
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• 제36권1호
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• pp.43-49
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• 1993

In order to survey possibility to produce saikosaponin from in vitro hairy root culture, culture of callus, adventitous root, and hairy root of Bupleurum falcatum L. were estabilished, and quantitative and qualitative aspects in saikosaponin extracted from these were compared with those of cultivated root. Callus grew well in MS medium containing 0.9 $\mu$M 2, 4-D. In contrast, both of adventitous root and hairy root grew well in hormone-free MS medium. However, hairy root showed more rapid growth with extensive lateral root branches, characteristics of lower content of water and softer than in adventitous root. Among the selected lines of adventitous root and hairy root were observed difference in the growth rate. Mannopine, one of opine synthesized in the transformed tissue with Agrobacterium rhizogenes. A4 were detected in the extract of hairy root lines. Pattern and content of crude saponin from adventitous and hairy root showed no difference, but somewhat difference from those of cultivated root. However, in callus, distinct production-aspect of saponin was not observed.

• Plant Resources
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• 제4권1호
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• pp.48-52
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• 2001

Seven genetic lines of Bupleurum falcatum L. from different geographical regions were analysed for saikosaponin contents and chromosomal numbers. The somatic chromosome numbers of B. falcatum originated from Euisong, Iri, Milyang, Sangnam, Taejon, and Youngchon were 2n=20 while Mishimasaiko showed 2n=26. However, chromosome features were different in plants grown in different geographical regions. Generally, Korean lines had higher saikosaponin contents than Mishimasaiko which is Japanese and Sangnam lines had highest saikosaponin contents compared to other tested lines.