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Characterization of Microbial Community in the Leachate Associated with the Decomposition of Entombed Pigs

  • Yang, Seung-Hak;Hong, Sun Hwa;Cho, Sung Back;Lim, Joung Soo;Bae, Sung Eun;Ahn, Heekwon;Lee, Eun Young
    • Journal of Microbiology and Biotechnology
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    • v.22 no.10
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    • pp.1330-1335
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    • 2012
  • Foot and mouth disease (FMD) is one of the acute infectious diseases in hoofed and even-toed mammals, including pigs, and it occurs via acute infection by Aphthovirus. When FMD is suspected, animals around the location of origin are typically slaughtered and buried. Other methods such as rendering, composting, and incineration have not been verified in practice in Korea. After the FMD incident, the regular monitoring of the microbial community is required, as microorganisms greatly modify the characteristics of the ecosystem in which they live. This is the result of their metabolic activities causing chemical changes to take place in the surrounding environment. In this study, we investigated changes in the microbial community during a 24 week period with DNA extracts from leachate, formed by the decomposition of buried pigs at a laboratory test site, using denaturing gradient gel electrophoresis (DGGE) with a genomic DNA. Our results revealed that Bacteroides coprosuis, which is common in pig excreta, and Sporanaerobacter acetigenes, which is a sulfur-reduced microbe, were continuously observed. During the early stages (0~2 weeks) of tissue decomposition, Clostridium cochlearium, Fusobacterium ulcerans, and Fusobacterium sp., which are involved in skin decomposition, were also observed. In addition, various microbes such as Turicibacter sanguinis, Clostridium haemolyticum, Bacteroides propionicifaciens, and Comamonas sp. were seen during the later stages (16~24 weeks). In particular, the number of existing microbial species gradually increased during the early stages, including the exponential phase, decreased during the middle stages, and then increased again during the later stages. Therefore, these results indicate that the decomposition of pigs continues for a long period of time and leachate is created continuously during this process. It is known that leachate can easily flow into the neighboring environment, so a long-term management plan is needed in burial locations for FMD-infected animals.

A Study on the Characteristics and Landscape Meaning Analysis of 'Gumi and Sipalgyung' in Jeongseon Gumijeongsa (정선 구미정사의 성격과 '구미(九美) 18경'의 경관의미)

  • Kim, Soonki;Rho, Jae-Hyun
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.34 no.2
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    • pp.63-74
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    • 2016
  • The purpose of this study is to expand a prospect of a few-remaining Byeolseo scenic sites of Gangwon-do(Province) through determining placeness formation, prospect characteristics, and landscape meanings of Gumi(Nine beauties) and Sipalgyung(18 scenic views) in Gumijeongsa. Gumijeongsa, which is located in a riverside of the Goljicheon(stream), a part of upstream of Namhangang, is the oldest existing Nujung architecture in the Jeongseon area. This study tried to unearth the Gumijonegsa's locational and formational characteristics through literature review and a field survey. Especially, through the field survey, this study tried to figure out the landscape meanings and contents of Gumi and Sipalgyung by Gumijeongsa's position and its prospect compositions. As a result of this study, Gumi and Sipalgyung of Gumijeongsa are variations of typical traditional culture of Gugok(Nine river bends) and Palgyung(Eight scenic views). Ja Lee, the founder, administrated Gumijeongsa as a core residence of Gugok culture. He established beautiful nine viewpoints from the building, named Gumi, and added 18 detailed landscapes(two landscapes for one beauties), instead of establishing nine river bends of the stream. The Gumi and Sipalgyung are formed by reflection of beautiful locational characteristics from the views in Gumijeongsa, thus the Gumi is the concept which is a unique variation of Gugok and Palgyung culture.

Spatial Analysis of the Confucian Cultural Landscapes at Jeongeuihyanggyo, Jeju Island (제주도 정의향교의 유교문화경관에 대한 공간분석)

  • Lee, HaengLyoul
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.35 no.4
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    • pp.29-42
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    • 2017
  • Jeongeuihyanggyo(旌義鄕校) is a kind of traditional, Confucian cultural landscapes that is located in Jeju special self-governing province. Its historical background illustrates that the first erection was started at Goseong-li of Seongsan-eup(城山邑 古城里) at Taejong(太宗) of 16 years(1417) and it moved to the west gate of Hyunseong(縣城) at Heojong(憲宗) of 15 years,(1849) Joseon. The aim at this study is to reveal characters of these Confucian Spatial Composition and Order, which are valuable resources that can be created into the cultural contents. The results demonstrate the characters of the Confucian cultural landscape as followings; the changing process of new building and its relocation, the utilization of antique maps in location interpretation of Jeongeuihyanggyo, Confucian symbolic elements of it, the spatial compositions and layouts of its buildings, physical structures, main buildings and their characters, locational points of topographic profile, changes of axial line at Daeseongjeon(大成殿), the D/H ratio of its courts. This study can show that Jeongeuihyanggyo is a case for realizing the Confucian reformation to enhance Seongeup Hyun's education standard considering the locality of the site which is an isolated island like Jejudo(濟州島). The author can see that the example of Confucian space is applied to various layout techniques, both horizontally and vertically, in a limited space condition of being in the castle. Therefore, it is necessary to revive this point so that it can utilize unique Confucian cultural landscape possessed only by Jeongeuihyanggyo.

Efficiency Analysis for RFID-based Curtain Wall of unit Type Construction (RFID 기술 적용에 따른 유닛타입 커튼월 공사의 효율성 분석)

  • Kang, Hyun-Koo;Ha, Young-Seo;Lim, Chul-Woo;Kim, Chang-Duk;Yu, Jung-Ho
    • Korean Journal of Construction Engineering and Management
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    • v.9 no.3
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    • pp.206-213
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    • 2008
  • Due to the following reasons, the management of curtain wall parts and material is very Important. Firstly, curtain wall work is one of the main works in High-rise building construction for it takes about $10{\sim}15%$ of the total construction cost. Secondly, the whole process of curtain wall work including manu acture, delivery, storage, installation and maintenance is very complicated and sometimes more than 30 companies involve in the process. Thus there are many control points for curtain wall units. Thirdly, there are not enough space on site for material storage and this situation is more serious for sites in urban area. The purpose of this research is to validate an information systems using RFID technology that is developed to manage the curtain wall units following the process of curtain wall work. For the validation, the cycle times of curtain wall work before and a ter the system was used. The results show that using the systems shorten the cycle time of the curtain wall work. Thus it is concluded that the system can increase the efficiency of managing curtain wall work.

An Uncertainty Analysis of Calculating Life Cycle Maintenance and Energy Costs for Technical Proposals (기술제안입찰을 위한 유지관리 및 에너지 비용 산출방식의 불확실성 분석)

  • Chung, Sung Young;Kim, Sean Hay
    • Korean Journal of Construction Engineering and Management
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    • v.19 no.5
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    • pp.3-9
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    • 2018
  • Although Life Cycle Cost (LCC) must be evaluated by experts, sometimes it may not allow a sufficient time for even the experienced LCC expert to make rational decisions. Therefore, it often ends with relatively comparing the final numbers. We have broken down 110 technical proposals that are actually bade and accepted for large construction projects, and then have analyzed the uncertainty of Maintenance and Energy (M&E) cost during building life cycle, which turns out be the most volatile factor in uncertainty of LCC. Also we suggest "Value Engineering Index (VEI)" - the reduced M&E cost that is normalized by the reduced first cost. It is analyzed that the most uncertain factors of the M&E cost include repair and replacement term differing from each project, duplicated repair and replacement, non-standard repair items, and site-specific energy cost. Eventually we propose a VEI population with a mean of 1.38 and a standard deviation of 1.19, which is obtained by individually and exclusively applying the uncertain factors of the M&E cost to the 35 standard sample of technical proposals. The LCC evaluators may be able to use the VEI population as the benchmark to select the technical proposal with the most reasonable LCC among many others in two suggested manners; the one is to deterministically calculate the probability of single VEIs, and the other is to stochastically calculate the probability of the VEIs where uncertainty is quantified.

An Analysis of the Relative Importance of Processes in the Closure Phase of Construction Program (건설프로그램 종결단계 업무프로세스의 상대적 중요도 분석)

  • Lee, Woo-Yeon;Lee, Seung-Hoon;Cha, Yongwoon;Hyun, Chang-Taek
    • Korean Journal of Construction Engineering and Management
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    • v.21 no.4
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    • pp.62-71
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    • 2020
  • Various studies on program management have been attempted due to recent changes in the environment of the construction market, and the importance of closure phase in each phase of the construction program is increasing. However, the reality is that the related research is insufficient and the field has been managed in a rule of thumb according to individual capabilities. Therefore, in order to successfully close the construction program and enhance satisfaction of stakeholders in the closure phase of the construction program, the key stakeholders were identified using the Power and Interest Matrix. The relative importance of the closure phase process was analyzed using AHP analysis method. It was also verified through on-site surveys and interviews about the proposed importance of the closure stakeholder and the relative importance of the process. It is expected that this study will increase the satisfaction of stakeholders and contribute to a successful closure if the user puts a lot of resources into the process that has a high priority in the closure phase of the construction program proposed in this study.

A Study on 'the Ecological Archive' in the Anthropocene (인류세 시대 '생태 아카이브' 구축에 관한 연구)

  • Lee, Kyong Rae
    • The Korean Journal of Archival Studies
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    • no.68
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    • pp.205-241
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    • 2021
  • This article explores how to incorporate the topic of the global environmental crisis called the "anthropocene" into archives studies and connect it to ecological practical reasons. In order to encourage discussion of archival studies, which puts the environmental crisis at a kind of archive constant value, this study seeks to examine the possibility of a quality shift in archival studies based on ecology. This article aims to go beyond the pragmatism of preparing improvements to eco-friendly record management, which is recently claimed by the "Green Archive" in Western archival studies. It calls for a new concept called 'ecological archive', which theoretically combines a more archives-based and ecological paradigm, and its epistemological transformation. Specifically, the ecological approach of archives is first discovered in the discussion of archival studies and at the same time, through the "ecological turn" of archives emphasized by recent anthropocene discourses, the concept of "ecological archive" emphasized by this article is embodied. This study uses 'ecological archive' as a universal and theoretical framework for archives as a basic concept for building ecological 'living' archives. In other words, for the construction of ecological archives, we reinterpret and extend so-called democratic values for archives, i.e., post-custodianship, community archives, and archives of emotions. Finally, the records of foot-and-mouth disease killing burial sites, an important site and example of the anthropocene tragedy, exemplifies the methodology of the actual application of ecological living archives. The case analysis aims to seek a new qualitative shift in record management that adapts to global ecological transformation, while also emphasizing the documentation by archival activism in ecological field practices jointly organized by archivists and citizens.

A Study on the Detoxification of Chrysotile and the use of High-density Extruded Cement Panel Reinforcement Fibers (백석면의 무해 섬유화 처리 방법과 고밀도 압출성형 패널 활용 연구)

  • Jang, Kyong-Pil;Kim, Tae-Hyoung;Song, Tae-Hyeob
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.9 no.2
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    • pp.223-228
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    • 2021
  • The final disposal method for asbestos building materials is to be landfilled at a designated waste landfill in accordance with the Waste Management Act. However, it is difficult to secure a domestic designated waste landfill site to landfill the entire amount of asbestos waste, which is expected to emit more than 400,000 ton/year by 2044. In this study, a detoxification treatment was performed on a ceiling tex with a density of 1.0 to 1.2g/cm3 containing 3 to 7% of chrysotile, and it was used as a reinforcing fiber for extruded panels. It was confirmed that asbestos components were detoxified through the reaction process using 30% oxalic acid and carbon dioxide, and it was recognized that these detoxifying properties were maintained even after extrusion molding. However, it was found that milling to a fiber size of less than 1mm for complete detoxification of asbestos resulted in a decrease in reinforcing performance. Therefore, in the case of using detoxified asbestos fibers in the extrusion molding process, it is considered desirable to add fibers with a length of 5mm or more to improve the reinforcing performance.

Comparison of the Perception of Each Participant on Quality Control Factor of Form Work (거푸집 공사 품질관리 요인에 대한 공사 참여 주체별 인식 비교)

  • Kim, Ju-Yong;Choi, Gyu-Jung;Park, Soon-Kyu;Shin, Yoon-Seok;Kim, Beak-Joong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.4
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    • pp.520-528
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    • 2021
  • In this study, we attempted to derive major management factors related to the quality control of formwork and identified differences in the perceptions of construction engineers, construction managers, and field workers about the importance and performance of the derived factors. As a result of the study, 18 management factors related to the formwork were derived. Among the derived factors, the field workers placed importance on the factors that influence the detailed process of the formwork, while construction engineers placed importance on the factors related to the judgment of the progress and completion of the construction. The construction managers placed importance on the factors that affect the quality of the building frame. Based on the results of this study, it is expected that a plan to improve the quality of formwork that accepts the various opinions of each participant will be established.

Correlations of Earthquake Accelerations and LPIs for Liquefaction Risk Mapping in Seoul & Gyeonggi-do Area based on Artificial Scenarios (서울, 경기지역의 시나리오별 액상화 위험지도 작성을 위한 지진가속도와 LPI 상관관계 분석)

  • Baek, Woohyun;Choi, Jaesoon
    • Journal of the Korean GEO-environmental Society
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    • v.20 no.5
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    • pp.5-12
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    • 2019
  • On November 15, 2017, a unpredictable liquefaction damage was occurred at the $M_L=5.4$ Pohang earthquake and after, many researches have been conducted in Korea. In Korea, where there were no cases of earthquake damage, it has been extremely neglectable in preparing earthquake risk maps and building earthquake systems that corresponded to prevention and preparation. Since it is almost impossible to observe signs and symptoms of drought, floods, and typhoons in advance, it is very effective to predict the impacts and magnitudes of seismic events. In this study, 14,040 borehole data were collected in the metropolitan area and liquefaction evaluation was performed using the amplification factor. Based on this data, liquefaction hazard maps were prepared for ground accelerations of 0.06 g, 0.14 g, 0.22 g, and 0.30 g, including 200years return period to 4,800years return period. Also, the correlation analysis between the earthquake acceleration and LPI was carried out to draw a real-time predictable liquefaction hazard map. As a result, 707 correlation equations in every cells in GIS map were proposed. Finally, the simulation for liquefaction risk mapping against artificial earthquake was performed in the metropolitan area using the proposed correlation equations.