• Journal of the Korean Society of Environmental Restoration Technology
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• v.25 no.2
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• pp.23-38
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• 2022

This study investigates the characteristics and physical habitat requirements for each Bufo gargarizans life history through a literature survey. After deriving variables for each component of Bufo gargarizans, in order to reduce regional deviations from eight previously studied literature research areas for deriving the criteria for variables, a total of 12 natural habitats of Bufo gargarizanss are selected as spatial ranges by selecting four additional sites such as Umyeonsan Ecological Park in Seoul, Wonheungibangjuk in Cheongju in the central region, Changnyeong Isan Reservoir in the southern region, and Mangwonji in Daegu. This study presents Bufo gargarizans SI, a species endemic to Korea, whose population is rapidly declining due to large-scale housing site development and road development, and develops a Bufo gargarizans HSI model accordingly to improve the function of the damaged Bufo gargarizans habitat and to present an objective basis for site selection of alternative habitat. At the same time, it provides basic data for adaptive management and follow-up monitoring. The three basic habitat requirements of amphibians, the physical habitat requirements of Bufo gargarizans, synthesized with shelter, food, and water, and the characteristics of each life history, are classified into five components by adding space and threats through literature research and expert advice. Variables are proposed by synthesizing and comparing the general characteristics of amphibians, among the previously studied single species of amphibians, the components of HSI of goldfrogs and Bufo gargarizans, and the ecological and physical environmental characteristics of Bufo gargarizans. Afterwards, through consultation with an amphibian expert, a total of 10 variables are finally presented by adjacent forest area(ha), the distance between spawning area and the nearest forest land(m), the soil, the distance from the wetland(m), the forest layered structure, the low grassland space, the permanent wetland area(ha), shoreline slope(%), PH, presence of predators, distance from road(m), presence or absence of obstacles. n order to derive the final criteria for each of the 10 variables, the criteria(alternative) for each variable are presented through geographic information analysis of the site survey area and field surveys of the previously studied literature research area. After a focus group interview(FGI) of 30 people related to the Bufo gargarizans colony in Cheongju, a questionnaire and in-depth interviews with three amphibians experts are conducted to verify and supplement the criteria for each final variable. Based on the finally developed Bufo gargarizans HSI, the Bufo gargarizans habitat model is presented through the SI graph model and the drawing centering on the Bufo gargarizans spawning area

• Korean Journal of Environment and Ecology
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• v.30 no.4
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• pp.705-711
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• 2016

In this experiment, embryos of Asian toad, Bufo gargarizans, were investigated to evaluate toxicity of chemicals along FETAX(Frog Embryo Teratogenesis Assay-Xenopus) protocol. Asian toad, Bufo gargarizans, embryos incubated and investigation of Zn and Benomyl effect by probit analysis. As a result, depends on the concentrations of Zn and Benomyl, mortality and malformation rates were increases and larval body length were decreased. The teratogenic concentration($EC_{50}$) of Zn and Benomyl were 2.3, $1.0mg/{\ell}$, respectively and the embryo lethal concentration($LC_{50}$) Zn and Benomyl were 10.3, 6.9, respectively. The teratogenic index(TI) were 4.4 in Zn and 6.7 in Benomyl, thus showed teratogenicity in embryonic development of B. gargarizans. These results reveal that Zn and Benomyl in this experiment suppressed the development of embryos at relatively low concentration. Much of B. gargarizans embryos can be secured, and easy to incubate. In addition, mortality, malformation ratios, malformation patterns and growth rates are similar to the results from the other assay systems. Therefore, the B. gargarizans embryo teratogenesis assay system could be a useful tool to evaluate toxicity of pollutants in environment.

• The Korean Journal of Zoology
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• v.17 no.2
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• pp.57-68
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• 1974

These experiments were performed in order to study histologically and histochemically on the epithelial cells of gall bladder in Carassius carassius, Bufo bufo gargarizans, Natrix tigrina lateralis, Urloncha striata var. domesticus and Bos taurus var. domesticus. The results of the observation were as follows: 1. There were different cell types in the epithelium of gall bladder in each animal and it could not be supported histochemically that the epithelia cells of gall bladder were divided into two cell types of the rod-shaped and barrel-shaped ones. 2. The epithelium of gall bladder in Carassius carassius, Bufo bufo gargarizans, Natrix tigrina lateralis, Urloncha striata var. domesticus and Bos taurus var. domesticus was simple columnar epithelium. 3. The eosinophilities of cytoplasm in the epithelial cells of gall bladder were in uniform stronger in the upper portion of nucleus in Carassius carassius, Bufo bufo gargarizans and Natrix tigrina lateralis than its other portions, and in Urloncha striata var. domesticus and Lepus cuniculus var. domesticus existed uniformly in all portions, but there were many non-eosinophilic cells in Bufo bufo gargarizans and many cells that weakly eosinophilic around nucleus in Bos taurus var. domesticus. 4. The periodic acid Schiff's reactivities in the epithelial cells of gall bladder were different in each other and the epithelial cells in PAS reaction were divided into two cell types of the dark and light ones. There presented the light cells of 6.4%, 4.3% and 3.7% of epithelial cell of gall bladder in Carassius carassius, Bufo bufo gargarizans and Urloncha striata var. domesticus for each other, but were not presented in Natrix tigrina lateralis and Bos taurus var. domesticus. 5. The ninhydrin-Schiff-active proteins were much in the epithelial cells of gall bladder in Bos taurus var. domesticus, Carassius carassius, Urloncha striata var. domesticus and Natrix tigrina lateralis in order and were much in epithelial cells in the upper portion of mucosal folds in Carassius carassius, Urloncha striata var. domesticus and Natrix tigrina lateralis, and the ninhydrin-Schiff-active protein of the epithelium of gall bladder in Bufo bufo gargarizans was uniformly distributed. 6. The epithelial cells of gall bladder in Carassius carassius, Natrix tigrina lateralis, Urloncha striata var. domesticus and Bos taurus var. domesticus had no stain reactivity or weak stain reactivity to neutral fat and all epithelial cells in Bufo bufo gargarizans had strong stain reactivity, though they were different in quantity of epithelial cell portion. 7. The stain reactivities to RNA and DNA were stronger in the epithelial cells of the upper portion of mucosal fold than in those of other portions.

• Animal cells and systems
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• v.12 no.2
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• pp.93-96
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• 2008

In recent years, we frequently observed missmatched pairs between male Asian toads, Bufo gargarizans, and bullfrogs, Rana catesbeiana, at the toad breeding ponds, where scramble competition for mating occurred among the male toads. Thus, we performed two-choice experiments to investigate recognition ability of mates and species in male toads. The test males did not discriminate sexes, but the clasped stimulus males immediately produced release calls and stopped it while the clasped stimulus female did not. In addition, the test male toads did not discriminate reproductive state of females and even species. However, male toads chose larger individuals. The present results indicate that the main reason of missmatched amplexus by the male toads is due to 1) the lack of recognition cues of conspecifics, 2) the lack of communication tools like release calls, and 3) the larger size of bullfrogs than male toads themselves.

• Proceedings of the Korean Society of Environment and Ecology Conference
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• 2009.10a
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• pp.195-198
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• 2009

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 2012.07a
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• pp.59-61
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• 2012

• Korean Journal of Veterinary Research
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• v.40 no.1
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• pp.17-26
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• 2000

The regional distribution and relative frequencies of endocrine cells were studied immunohistochemically (PAP methods) in the alimentary tract and pancreas of the toad, Bufo bufo gargarizans Cantor using specific antisera against bovine Sp-1/chromogranin (BCG), serotonin, bombesin, gastrin, substance P (SP), somatostatin, insulin, glucagon, pancreatic polypeptide (PP), vasoactive intestinal polypeptide (VIP) and secretin. Nine kinds of endocrine cells were identified in this study. Spherical or spindleshaped immunoreactive (IR) cells were located in the gastric glands of stomach regions, in the basal portion of the epithelium of intestinal tract or esophagus, and in the exocrine or pancreatic islets with variable frequencies. In the alimentary tract, BCG-IR cells were found in the fundus and pylorus with rare and a few frequencies, respectively. Serotonin-IR cells were demonstrated in the whole alimentary tract including the esophagus. Bombesin- and SP-IR cells were restricted to the stomach regions and gastrin-IR cells were restricted to the pylorus. Somatostatin-IR cells were detected throughout the whole alimentary tract except for the large intestine, However, insulin-, glucagon-, PP-, VIP- and secretin-IR cells were not detected in the alimentary tract. In the pancreas of toad, the distribution and relative frequency of endocrine cells were similar to those of other mammals. Insulin-IR cells were located in the central portion of the pancreatic islets and interspaces of exocrine portions, and glucagon-, somatostatin- and PP-IR cells were detected in the marginal regions of the pancreatic islets and interspaces of exocrine. However, other IR cells were not found in the pancreas. In conclusion, the regional distribution and relative frequency of the endocrine cells in the alimentary tract and pancreas of the toad were similar to other anuran species but some differences which might be caused by feeding habits and species specification were also observed.

• Korean Journal of Environmental Biology
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• v.31 no.4
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• pp.340-346
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• 2013

We have confirmed the first case of mass mortality which occurred in the wild populations of larval toad, Bufo bufo gargarizans during 2007~2009 at Mangwel pond, which is a long time breeding site, and is located at the south eastern area in Daegu. We have investigated through microbiological tests the cause of mass decease of the larval toad, and detected numerous bacteria from the intestine by histological examines and cultures. The pathogen isolated from the infected larval toads has been identified as Aeromonas hydrophila, which is widely distributed in aquatic environment. However, this phenomenon of mass lethal pattern of the larval toad disappeared at the breeding pond after maintaining better water levels and low temperature in spring during breeding season in 2010. We propose that the high temperatures during the 2007~2009 breeding season resulted in lower water levels and drier conditions leading to explosive propagation of A. hydrophila in the pond which impacted the larval toad's immune function.

• Korean Journal of Environment and Ecology
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• v.29 no.4
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• pp.525-532
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• 2015

In order to determine the testicular cycle of the Asian toad, Bufo gargarizans, adult males of the species were captured around Jeongeup city (Jeollabuk-do, Korea) during March, 2012 to February, 2013 and the gonadosomatic index (GSI) and the changes of germ cells in their testes were investigated throughout the year. The study indicated that the spermatogenesis in the seminiferous tubule of testes began in April and became most active in July. The recorded GSI was the highest and the cross area of seminiferous tubule was the widest in this period. The seminiferous tubules at the post spawning stage appeared in February, the largest amounts occurred in March and primary spermatogonia also appeared in this period. The GSI and the cross area of seminiferous tubules were found to be the lowest in March, indicating a testicular cycle with potentially continuous spermatogenic process. According to the findings above, it is confirmed that testicular spermatogenesis takes place actively between April to July in male Asian toad and that their breeding season is February to March.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.706-714
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• 2017

An investigation of the effects of Pb for domestic anuran embryos were evaluated with the Frog Embryo Teratogenesis Assay; Xenopus (FETAX). Depending on the species, the difference between the embryo size and the embryonic development time was determined. As a result, mortality and malformation rates were increased, malformation patterns were changed and larval body length were decreased in a dose dependent manner of the Pb. The half maximal lethal concentration ($LC_{50}$) of the Bufo gargarizans, Hyla japonica, Rana nigromaculata and Bombina orientalis were 0.58, 0.49, 0.52, $0.54mg\;L^{-1}$, respectively. The half maximal effective concentration ($EC_{50}$) of the Bufo gargarizans, Hyla japonica, Rana nigromaculata and Bombina orientalis were 0.35, 0.74, 0.30, $0.29mg\;L^{-1}$, respectively. The teratogenic index (TI) were 1.66 in the Bufo gargarizans, 1.81 in the Hyla japonica, 1.73 in the Rana nigromaculata and 1.86 in the Bombina orientalis, respectively. Therefore, the Pb seems likely to have a teratogenic effect in all four species' embryonic development. The Bombina orientalis was the most sensitive to the Pb. This means that the difference between the different species, even if they have all been exposed to the same concentration of pollutants depending on the species. The result above show that the Pb acts as a teratogenic agent in the development of the four domestic frog species.