• Title/Summary/Keyword: Buffer Solution

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Optimum Concentration of the Cd(II)-Quercetin Complexation Reaction (Quercetin의 카드뮴 착물반응에 대한 최적농도)

  • Lee, Jeong-Ho;Shin, Sun-Woo;Baek, Seung-Hwa
    • YAKHAK HOEJI
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    • v.53 no.5
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    • pp.235-240
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    • 2009
  • The interaction of cadmium (II) ion with quercetin was investigated in aqueous solution at different pH. The quercetin/cadmium stochiometries for cadmium (II) binding have been determined by UV-vis spectrophotometric method. The complexation of Cd(II) ion with 54.72 ${\mu}M$ quercetin (A=1.00793) was formed in 0.2 M $NH_3-0.2$ M $NH_4Cl$ (pH 8.0) buffer solution. 1:1 Cd(II)-complex had a maximum absorbance and showed the bathochromic shift of the long-wavelength band of the UV-vis spectra in the alkaline pH when interacted with quercetin in buffer solution. These results suggest that Cd(II)-quercetin complex has the optimal condition of chelation in basic buffer solution.

Sensitivity of a charge-detecting label-free DNA sensor using field-effect transistors (FETs) depending on the Debye length (전계효과 트랜지스터(FETs)를 이용한 전하 검출형 DNA 센서에서 Debye length에 따른 검출 감도)

  • Song, Kwang-Soup
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.48 no.2
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    • pp.86-90
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    • 2011
  • The effects of cations are very important in field-effect transistors (FETs) type DNA sensors detecting the intrinsic negative charge between single-stranded DNA and double-stranded DNA without labeling, because the intrinsic negative charge of DNA is neutralized by cations in electrolyte solution. We consider the Debye length, which depends on the concentration of cations in solution, to detect DNA hybridization based on the intrinsic negative charge of DNA. The Debye length is longer in buffer solution with a lower concentration of NaCl and the intrinsic negative charge of DNA is more effective on the channel surface in longer Debye length solution. The shifts in the gate voltage by DNA hybridization with complementary target DNA are 21 mV in 1 mM NaCl buffer solution, 7.2 mV in 10 mM NaCl buffer solution, and 5.1 mV in 100 mM NaCl buffer solution. The sensitivity of FETs to detect DNA hybridization based on charge detection without labeling depends on the Debye length.

Coating of ZnS phosphor by $SiO_2$ sol-gel

  • Lee, You-Hui;Han, Sang-Do;Han, Chi-Hwan;Yang, Hua;Singh, Ishwa
    • 한국정보디스플레이학회:학술대회논문집
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    • 2004.08a
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    • pp.719-723
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    • 2004
  • Silica coating on ZnS particles with buffer solution has been investigated. Diluted sodium silicate in water was used as the precursor material and it was diluted in water. Sodium silicate was added drop-wise in the continuously stirred suspension of ZnS in the buffer solution at room temperature. Smooth and evenly distributed silica coated ZnS phosphors has been obtained when the pH of buffer solution was 10, the concentration of sodium silicate in water was 20 wt%, firing temperature was 500 $^{\circ}C$.

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Effects of Mo on the Passive Films Formed on Ni-(15, 30)Cr-5Mo Alloys in pH 8.5 Buffer Solution

  • Jang, Hee-Jin;Kwon, Hyuk-Sang
    • Journal of the Korean Electrochemical Society
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    • v.12 no.3
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    • pp.258-262
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    • 2009
  • The composition and semiconducting properties of the passive films formed on Ni- (15, 30)Cr-5Mo alloys in pH 8.5 buffer solution were examined. The depth concentration profile of passive films formed on Ni-(15, 30)Cr-5Mo in pH 8.5 buffer solution showed that Mo enhances the enrichment of Cr. The Mott-Schottky plot for the passive film on Ni-(15, 30)Cr- 5Mo closely resembled that for the film on Cr, whereas those for the less Cr-enriched film on Mo-free alloys showed similar behavior to that for the film on Ni. The acceptor density was reduced by increasing Cr content in Ni-(15, 30)Cr-(0, 5)Mo alloys, but addition of Mo considerably increased the acceptor density.

Effects of Ionic Strength in the Medium on Sample Preconcentration Utilizing Nano-interstices between Self-Assembled Monolayers of Gold Nanoparticles

  • Nguyen, Ngoc-Viet;Wu, Jian-Sheng;Jen, Chun-Ping
    • BioChip Journal
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    • v.12 no.4
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    • pp.317-325
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    • 2018
  • This paper investigated the effects of ionic strength in the medium on a preconcentrator for a protein sample with low concentration. The preconcentration chip was designed and fabricated using a polydimethylsiloxane replica through standard lithophotography. A glass substrate is silanized prior to functionalizing the nanoparticles for self-assembly at a designed region. Due to the overlap of electrical double layers in a nanofluidic channel, a concentration polarization effect can be achieved using an electric field. A nonlinear electrokinetic flow is induced, resulting in the fast accumulation of proteins in front of the induced ionic depletion zone, so called exclusion-enrichment effect. Thus, the protein sample can be driven by electroosmotic flow and accumulated at a specific location. The chip is used to collect fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) diluted in phosphate-buffered saline (PBS) buffer solution. Different concentrations of the buffer media were studied herein. Fluorescence intensity images show that the buffer concentration of 4 mM is more appropriate than all the other ones. The sample of FITC-BSA with an initial concentration of $10{\mu}M$ in the 4 mM PBS solution increases its concentration at the desired region by up to 50 times within 30 min, demonstrating the results in this investigation.

Hardening and Hydroxyapatite Formation of Bioactive Cement Prepared from Calcium Phosphosilicate Glass

  • Kim, Cheol-Young;Park, Sang-Jong
    • The Korean Journal of Ceramics
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    • v.1 no.3
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    • pp.131-136
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    • 1995
  • It has been reported that the biocement obtained by mixing $CaO-SiO_2-P_2O_5$ glass powders with ammonium phosphate solution has biocompatibility as will as high strength. The hardening mechanism and hydroxyapatite forming mechanism were discussed when $53.6%CaO_1,\; 38.1%SiO_2,\; 7.7P_2O_6,\; 0.6%CaF_2$(mole %) glass powder was reacted with ammonium phosphate solution and reacted in tris-buffer solution, respectively. High strength hardened biocement was obtained for the specimen with $CaNH_4PO_4\;H_2O$ crystal when the glass powder was mixed with ammonium phosphate solution, and hydroxyapatite crystal was rapidly formed only in the sample with $CaNH_4PO_4\;H_2O$ crystal when it was reacted in tris-buffer solution.

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High-precision THz Dielectric Spectroscopy of Tris-HCl Buffer

  • Lee, Soonsung;Kang, Hyeona;Do, Youngwoong;Lee, Gyuseok;Kim, Jinwoo;Han, Haewook
    • Journal of the Optical Society of Korea
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    • v.20 no.3
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    • pp.431-434
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    • 2016
  • Tris-HCl buffer solution is extensively used in biochemistry and molecular biology to maintain a stable pH for biomolecules such as nucleic acids and proteins. Here we report on the high-precision THz dielectric spectroscopy of a 10 mM Tris-HCl buffer. Using a double Debye model, including conductivity of ionic species, we measured the complex dielectric functions of Tris-HCl buffer. The fast relaxation time of water molecules in Tris-HCl buffer is ~20% longer than that in pure water while the slow relaxation time changes little. This means that the reorientation dynamics of Tris-HCl buffer with such a low Tris concentration is quite different from that of pure water.

Effect of Buffers on Citric Acid Production by Aspergillus niger NRRL 567 in Solid Substrate Fermentation (Aspergillus niger NRRL 567을 이용한 고체배양에서 완충용액이 구연산 생산에 미치는 영향)

  • Kim, Jin-Woo
    • Korean Chemical Engineering Research
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    • v.50 no.5
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    • pp.874-878
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    • 2012
  • In the submerged fermentation of fungi, it was known pH had significant effect on the citric acid production. Various growth conditions were applied with different buffer on citric acid production by Aspergillus niger NRRL 567 grown on peat moss to find the optimum pH and most effective buffer solution. The initial pHs of different buffer solutions significantly influenced on the citric acid production and A. niger NRRL 567 produced citric acid more efficiently at high pHs. A phosphate buffer and a carbonate buffer with pH 8.6 and pH 10.0 were identified as suitable buffer solutions for citric acid production. The maximal citric acid production of 564.3 g/kg solid substrate was achieved employing carbonate buffer at pH 10.0.

An Exponentialization Procedure for General FMS Network of Queues with Limited Buffer

  • Lee, Ho-Chang
    • Journal of the Korean Operations Research and Management Science Society
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    • v.19 no.3
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    • pp.203-217
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    • 1994
  • In this paper we develop an exponentialization procedure for the modelling of open FMS networks with general processing time at each station and limited buffer size. By imposing a reasonable assumption on the solution set, the nonlinear equation system for the exponentialization procedure is formulated as a variational inequality problem and the solution existence is examined. The efficient algorithm for the nonlinear equation system is developed using linearized Jacobi approximation method.

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Dyeing Properties of Rose Flower Extracts on Silk Fabrics (장미꽃 추출물에 의한 견직물의 염색성)

  • Nam Sung Woo
    • Textile Coloration and Finishing
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    • v.16 no.6
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    • pp.10-15
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    • 2004
  • The colorants were extracted from the flower leaf of rose using a buffer solution. Dyeing properties and the fastness of silk fabrics dyed with rose flower extracts were investigated. K/S values of dyed fabrics were increased as the concentration of rose flower extracts was increased. Optimum dyeing temperature of rose flower extracts was $30^{\circ}C$. Fastness were generally good except light fastness which was extremely poor.