• Journal of Biosystems Engineering
• /
• 제24권1호
• /
• pp.67-74
• /
• 1999

A prototype cut-flower sorter was developed and tested for its performance with five varieties of roses. Support plates driven by a chain mechanism transported the roses into an image inspection chamber. Color image processing algorithms were developed to evaluate the length, thickness, and straightness of stem and color, height, and maturity of bud. The average absolute errors of the system for the measurements of stem length, stem thickness, and height of bud were 19.7 mm, 0.5 mm, and 3.8 mm, respectively. The results of classification by the sorter were compared with those of a human inspector for straightness of stem and maturity of bud. The classification error for the straightness of stem was 8.6%, when both direct image and reflected image by a mirror were analyzed. The accuracy in classifying the maturity of bud varied among the varieties, the smallest for‘Nobless’(1.5%) and the largest for‘Rote Rose’(13.5%). The time required to process a rose averaged 2.06 seconds, equivalent to the capacity of 1,600 roses per hour.

• 대한치과교정학회지
• /
• 제26권6호
• /
• pp.667-676
• /
• 1996

We have examined the in vitro stage-related chondrogenic potential of human mandibular and limb bud mesenchyme cells using micromass culture. Our results indicate that limb bud mesenchyme cells as early as stage 16 by Carnegie system (37 days), well before the initiation of in vivo chondrogenesis, have chondrogenic potential which is expressed in micromass culture. These results are correlated with stage-related chondrogenic potential of human limb bud in vivo as a result of Alcian blue staining. The proliferation of chondrogenic cells increased in the first 3 days after culture and then decreased. These results were correlated with the cell cycle analysis of which the number of $G_0^1/G_1$ phase increased markedly after 3 days of culture, while the percentage of cells in S phase was decreased. On the other hand, it was rarely differentiated in the mandible. We examined the effects of two PKC modulators such as phorbol 12-myristate 13-acetate (PMA), a potent activator of PKC, and staurosporine (STSN), an inhibitor of PKC. PMA inhibited the chondrogenesis, whereas STSN promoted the chondrogenesis in a dose dependent manner. In addition, PMA exerted no inhibitory effect when the cells were pretreated for 24 h with STSN, implying that the chondrogenic events might be settled at an early step in vitro and FKC may act as a negative modulator. Collectively, these results demonstrate, for the first time, the stage-related chondrogenic potential of human mandibular and limb bud mesenchyme cells and the role of PKC during chondrogenesis in vitro & in vivo.

• International Journal of Oral Biology
• /
• 제33권3호
• /
• pp.83-89
• /
• 2008

Glutamate-induced cobalt uptake reveals non-NMDA glutamate receptors (GluRs) in rat taste bud cells. Previous studies suggest that glutamate-induced cobalt uptake in taste cells occurs mainly via kainate type GluRs. Cobaltstained cells were immunoreactive against GluR6 and KA1 subunits of GluRs. However, the functions of those type of receptors are not known yet. It is important question which types of taste cells are cobalt-stained when stimulated by glutamate and whether they express these kinds of GluRs. Circumvallate and foliate papilla of Sprague-Dawley rats (45-60 days old) were used. A cobalt-staining technique combined with immunohistochemistry against specific markers for taste bud cell types, such as blood group H antigen (BGH), $\alpha$-gustducin (Gus), or neural cell adhesion molecule (NCAM) was employed. We also performed double labeling of GluR6 or KA1 subunits of GluR with each specific marker for taste bud cell types. Lots of cobaltstained taste bud cells expressed Gus-like immunoreactivity, and subsets of the cobalt stained cells appeared NCAM- or BGH-like immunoreactivity. Stimulation with 1 mM glutamate significantly increased the number of cobaltstained cells in Gus-like immunoreactive cells, but not in NCAM- or BGH-like immunoreactive cells. In the double labeling experiments, GluR6 and KA1 subunits of GluRs were mainly expressed with Gus. These results suggest that kainate glutamate receptors preferentially expressed in type II taste bud cells in rat.

• 한국작물학회지
• /
• 제27권3호
• /
• pp.193-197
• /
• 1982

수도의 동일요소에 형성된 각 관근의 신장량은 다음과 같은 관계에 있음을 알았다. 1. 분벽아를 제거하여 각 관근만을 sink로 하였을 경우에는 중륵의 가까운 곳에 형성된 관근이 분벽아쪽에 형성된 관근보다 신장량이 좋았다. 2. 분벽아가 관근과 같이 sink로서 존재할 경우, 전출엽요소에서 출근한 관근이 가장 길었으며 또 분벽경 부근에 형성된 관근이 그 다음이었고 중륵 쪽에 형성된 관근이 가장 짧았다.

• 원예과학기술지
• /
• 제34권3호
• /
• pp.495-509
• /
• 2016

Irrigation applications (IA) and increased bud load (BL) are fundamental practices that are used to achieve optimum yields in grape production, while maintaining fruit quality parameters. Two different irrigation amounts (IA-I and IA-II) based on growth stages, in addition to a non-irrigated (rain-fed) control, along with two different BL applications [normal bud load based on traditional pruning practices (1BL) and double bud load (2BL)] were evaluated over a two-year experiment for their effects on the biochemical composition of the berries. Berries from the rain-fed vines had higher sugar levels, whereas no significant change was detected in organic acid levels. The increased bud load (2BL) treatment yielded less sugar in the berries compared to the 1BL control in both years. The total non-colored phenolic compounds (NPC) were greater in the irrigated (especially IA-I) and 2BL treatments than in those of the rain-fed and 1BL control. However, total anthocyanin was greater in the non-irrigated and 1BL control than in the irrigated and 2BL treatments. The antioxidant contents of the berries also varied according to the treatments and years. Our results implied that implementing a higher bud load along with the IA-I irrigation application, in which irrigation applications were 50 and 75% of the cumulative evaporation from the Class A pan during berry set to veraison and veraison to harvest growth stages, respectively, can help in obtaining greater yields in high-plateau viticulture. Thus, if more buds are left on the vines, along with sufficient irrigation and rainfall, yield may increase while maintaining or increasing the biochemical composition of the berries.

• Journal of Plant Biotechnology
• /
• 제48권1호
• /
• pp.54-61
• /
• 2021

This work describe an efficient method for the shoot induction and plant regeneration of seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim. The highest rate of shoot induction (82.2%) was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BAP). However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. In contrast, the longest average shoot length (3.0 cm) was observed after growth on MS medium with 2.0 mg/L zeatin. No multiplication occurred when apical bud explants were cultured with kinetin-supplemented media. During rooting of in vitro-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5 ~ 1.0 mg/L indole-3-butyric acid (IBA) or 3.0 mg/L 1-naphthaleneacetic acid (NAA). During the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)-supplemented medium had the highest survival rate (100%) and maximum root length (18.5 cm). These findings suggest that a low concentration (0.5 mg/L) of IBA is appropriate for the rooting and acclimatization of T. mandshurica. Plants were successfully transferred to the greenhouse with a 100% survival rate. This protocol will be useful for the large-scale propagation of Tilia species.

• 원예과학기술지
• /
• 제29권3호
• /
• pp.181-186
• /
• 2011

포도 '캠벨얼리'의 발아기 예측을 위해 자발 휴면 타파 이후 발아일까지의 발육 속도(DVR) 계산식을 도출하였다. 항온 실험에서 계산한 DVR은 온도에 대하여 지수 함수식 또는 선형 함수식 모형으로 증가하는 경향이며 DVR 기울기는 0.0019 내외였다. 포도 DVR 계산식은 대기 온도에 대하여 $DVR=0.0249+0.0020e^{0.1654x}$ 또는 DVR = 0.0019x + 0.0187이었으며, DVR 계산식에 일 평균 기온을 대입하여 계산한 예측 발아일의 실측 발아일에 대한 적합도 검정 RMSE 값이 4일 이하로 작았다. 또한 DVR 계산식에 시간별 온도 자료를 대입한 경우에는 일 평균 기온 자료를 사용한 경우보다 오차 값이 작았으며 이때 RMSE 값은 3일 이하였다. 이는 본 연구에서 계산한 DVR 계산식이 포도 발아기 예측에 유용한 예측 값을 제공할 수 있다는 것을 의미한다.

• 한국농림기상학회지
• /
• 제14권4호
• /
• pp.229-235
• /
• 2012

본 연구는 소나무 산지의 개엽시기에 영향을 미치는 기후인자를 구명하기 위해 수행되었는데 1996년 국립산림과학원에서 조성한 정선, 충주, 제주의 소나무 산지시험림에서 조사가 이루어졌다. 휴면 중이던 동아가 부풀어 오르는 시점을 생장개시기로, 신초가 포린을 뚫고 나와 0.5mm 정도 자란 시점을 개엽시기로 정해 2004년 3월말부터 5월 중순까지 2일 간격으로 조사를했다. 개엽시기에 대한 산지 기후인자의 영향을 구명하기 위해 조림지와 산지의 기후인자를 구했으며, 각 기후인자의 생태적거리를 구해 공준상관 분석을 실시했다. 조림지별 생장개시기와 개엽시기는 제주, 충주, 정선 조림지 순으로 빨라 조림지의 평균 기온 차이에 따른 구배를 보여주었다. 종자산지의 기후인자 차이가 조림지에서의 생장개시기 및 개엽시기에 미치는 영향을 구명하기 위해 생태적거리 자료를 이용하여 공준상관 분석을 실시한 결과, 조림지와 산지 간 위도, 경도, 12~2월 최저기온, 11~2월 최고기온 그리고 생육기간 차이가 주요 인자인 것으로 추정되었다. 결론적으로 조림지보다 겨울철 기온이 낮은 곳에서 온 산지가 생장개시 및 개엽시기가 빠른 전형적인 북-남 또는 저온-고온 경사변이를 나타내 소나무 산지에 따른 지리적 유전적 변이가 존재하는 것을 확인할 수 있었다.

• 식물조직배양학회지
• /
• 제28권3호
• /
• pp.173-173
• /
• 2001

• 생물환경조절학회지
• /
• 제15권3호
• /
• pp.283-288
• /
• 2006

Chicory(Cichorium intybus L.)의 우량 종근 생산을 위해 파종 시기를 1개월 간격으로 하여 종근 형성 및 화아분화에 미치는 영향을 조사하였다. 화아분화 개시는 4월 파종은 6월 17일, 5월 파종은 7월 2일, 6월 파종은 7월 30일, 7월 파종은 10월 1일에 각각 화아가 분화되었으나 8월과 9월 파종은 전혀 분화되지 않았다. 파종시기별 생육특성에서 $4{\sim}7$월 파종은 종근의 무게가 150g에 도달하기 전에 화아가 분화되어 종근을 이용할 수가 없었으며, 9월 파종은 생육동안 외기온도가 낮아 겨울을 넘기면서 종근이 정상적으로 발육하지 못하는 결과를 보였고, 8월 파종은 파종 후 약 90일이 되는 시기에 Chicon을 생산할 수 있는 종근의 적절한 중량인 200g이상의 이상적인 굴취기에 도달하였으며, 생육기간동안 화아분화가 일어나지 않았다. 따라서 우리나라 기후에 따른 우량 Chicory 종근을 생산하기 위한 적정 파종기는 8월인 것으로 조사되었다.