• 한국동물위생학회지
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• 제24권1호
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• pp.9-14
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• 2001

This study was carried out to investigate the prevalence of Neospora caninum infection in dairy cow and Korean native cattle(KNC), raised in several Chungnam province. To determine the prevalence of antibodies to N caninum, a total of five hundred fifty six sera were analyzed by indirected fluorescent antibody(IFA) test. Five hundred thirty three sera were collected from fifteen dairy herds and twenty three sera were taken from fourteen KNC herds from December 1999 to November 2000. Seropositive ratio of the dairy cattle sera were individually or herdly tested and showed 64.2% and 93.3%, respectively. It was recorded with 78.6% and 47.8% in KNC. The seropositive ratio of dairy cattle was depended on the size of ranch. It was 92.2, 60.7 and 57.9% at the size of less than thirty, thirty to seventy and more than seventy one cattle, respectively However, it was different from the province of Chungnam. The seropositive ratio to N caninum of dairy cattle were 79.5, 53.1, 61.4 and 31.1% at Gongju, Yeongi, Geumsan and Cheongwon, respectively. It showed difference at the growth stage and sex of cattle. The seropositive ratios of N caninum of calf, heifer, premiparous, multiparous(2nd-5th), multiparous (6>th) and bulls confirmed to 25.0, 50.3, 70.3, 71.2, 50.0 and 50.0%, respectively. It was related with brucellosis in cattle. The infected ones with brucellosis were 75.7% of seropositive ratios to N caninum. The results of this study indicated that N caninum infection was widespread in Chungnam province and confirmed existing with brucellosis in cattle.

• 한국동물위생학회지
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• 제30권3호
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• pp.375-384
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• 2007

This study was conducted to investigate the characteristics of brucellosis in Korean native cattle in a farm where bovine brucellosis was confirmed 3 times from September 2006 to March 2007. Of 74 bulls serum samples examined, 21 (28.4%) were positive by Rose-Bengal test (RBT) and Standard tube agglutination test (STAT). In the isolation test from seropositive bulls, B abortus was isolated and identified from 2 specimens (testis, intestinal lymph node) among 6 kinds of specimens including blood, urine, feces and soil. Isolation rate of intestinal lymph node and testis was 25% (3/12 cases) and 16.7% (2/12), respectively. B abortus was also isolated from calves below 12 months old, i.e., 1 isolate (25.0%) was confirmed from testis, 4 (40.0%) from supra-mammary lymph nodes and 1 (25.0%) from intestinal lymph node. All isolates had Brucella specific 16s r-RNA with 905-bp band detected by PCR assay. For the more effective control of bovine brucellosis in korea, this paper would like to suggest that all of bulls and calves should be included in the screening tests.

• Clinical and Experimental Vaccine Research
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• 제12권4호
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• pp.304-312
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• 2023

Purpose: Due to the many problems with commercially available vaccines, the production of effective vaccines against brucellosis is a necessity. The aim of this study was to evaluate the immune responses caused by the chimeric protein consisting of trigger factor, Bp26, and Omp31 (TBO) along with aluminum hydroxide (AH/TBO) and selenium (Se/TBO) nanoparticles (NPs) as adjuvants in mouse model. Materials and Methods: Recombinant antigen expression was induced in Escherichia coli BL21 (DE3) bacteria using IPTG (isopropyl-d-1-thiogalactopyranoside). Purification and characterization of recombinant protein was conducted through NiFe₃O₄ NPs, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western blot. NP characteristics, including morphology and particle size, were measured in vitro. The recombinant TBO was loaded on to AH and Se NPs and were administered subcutaneously. After mice immunization, measurement of antibody titter and protection assay was performed. Results: The average sizes of AH and Se NPs were about 60 nm and 150 nm, respectively. The enzyme-linked immunosorbent assay results showed that the serum of mice immunized by subcutaneous injection with both nanovaccines produced significant immunoglobulin G (IgG) responses against the chimeric antigen. The results of TBO-specific IgG isotype (IgG2a/IgG1) analysis showed that both AH and Se NPs induced a type to T-helper immune response. In addition, the results of the challenge with the pathogenic strain of Brucella melitensis 16M showed that vaccinated mice with AH/TBO NPs indicated a higher reduction of bacterial culture than immunized mice with Se/TBO NPs and TBO alone. Conclusion: The results showed that AH NPs carrying chimeric antigen can be a promising vaccine candidate against brucellosis by producing protective immunity.

• 한국식품과학회지
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• 제5권2호
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• pp.108-112
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• 1973

저자(著者)는 식품(食品)에 기인(起因)하는 질환(疾患)의 요인(要因)이 될 수 있는 식품위생학상(食品衛生學上) 중요(重要)한 zoonoses 의 국내발생(國內發生) 양상(樣相)을 알아 보고자 시도(施導)하여 국내(國內)의 각종(各種) 기초자료(基礎資料)를 분석(分析) 통계처리(統計處理) 하여 보았든 바 다음의 몇가지 결과(結果)를 얻었다. (1) 국내(國內)에서 발생(發生)한 사람에서의 zoonoses 는 1962년(年)부터 1966년도(年度)까지 5 개년(個年) 간(間)에 anthrax 3 예(例), brucellosis 2 예(例), erysipeloid 140 예(例) 로서 앞으로 계속적인 조사(調査)가 요구(要求) 되었다. (2) 국내(國內)에는 670 개소(個所)의 도축장(屠畜場)이 있으며, 연도별(年度別) 도축검사중(屠畜檢査中) 불허건수(不許件數)는 과거(過去) 10 개년간(個年間)에 우(牛)가 전도축우(全屠畜牛)의 $0.45{\sim}0.98%$, 돈(豚)가 $0.005{\sim}0.05%$로서 돈(豚)보다 우(牛)의 경우가 많았다. (3) Epidemiological pattern 별(別) 국내발생(國內發生) zoonoses 를 가축(家畜)에서 조사(調査)한 바에 의(依)하면; (a) Direct-zoonoses 로서 anthrax, brucellosis 및 erysipelas 가 있었으며, 우(牛)의 anthrax 가 최고발병율(最高發病率)로는 1964년도(年度)의 1.7(per 100,000)이었고, 유우(乳牛)의 brucellosis는 1960 년도(年度)의 1338.4, 돈(豚)의 erysipelas 는 1962 년도(年度)의 1897.2 이었다. (b) Cyclo-zoonoses 중(中) 우리나라 돈(豚)의 cysticercus cellulosae 의 감염율(感染率)은 육지(陸地)의 경우 $1.0{\sim}2.7%$ 제주도(濟州道)의 경우 $3.8{\sim}8.5%$까지 감염율(感染率)이 있음을 문헌(文獻)에 의(依)하여 알 수 있었다.

• 한국동물위생학회지
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• 제30권2호
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• pp.243-249
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• 2007

The tube agglutination test has been used for bovine brucellosis diagnosis in Korea since middle 1950s. The reported high specificity was its value in eradication program. However, the reading of reaction mostly depends on personal experience, thus here we report a way to improve accuracy and uniformity of reading. The tube agglutination was conducted according to the protocol provided by Korean Ministry of Agriculture and Forestry. The intensity of reaction was measured by spectrophotometer. The relationship between turbidity and percentage clearing was generally in direct proportion and linear. The correspondent percent transmittance at 75, 50, and 25% clearing were 91, 82, and 73, respectively. Then, the degree of percentage of clearing at given international unit was measured. With about 1.5 unit of serum, the maximum percentage clearing was observed. The international unit showing 25, 50, and 75 percentage clearing were 0.61, 0.83 and 1.35, respectively. Based on the information obtained using international standard serum, the calculation of international unit of test serum was available. According to the protocol for bovine brucellosis diagnosis which provided by Korean Ministry of Agriculture and Forestry, the available range of detectable international unit was between 15 and 538. And the corresponding international units for suspicious case ranged between 42 and 127. Of the 35 sera from B abortus infected cattle, about half of them had more than 538 international units. Collectively, the reading of turbidity using spectrophotometer and application to international unit improved accuracy and uniformity of reading.

• 대한수의사회지
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• 제19권2호
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• pp.27-33
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• 1983

A total of 110 adult dogs (12pet dogs, 27 stray dogs, 71 other dogs collected from were examined for Brucella canis infection, and 11cases ($10\%$) were found to have Seoul areal high titered agglutinin. Especially, the positive titer (1 : 160

• 대한수의사회지
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• 제18권5호
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• pp.54-59
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• 1982

Now in Korea Brucellosis was diagnosed by individual periodic blood tests. Author hope that individual periodic blood tests was performed after Milk Ring Test, because if Cattles show negative reaction to Milk Ring Test, Periodic blood tests are needless.

• 대한수의학회지
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• 제52권1호
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• pp.25-31
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• 2012

Brucella (B.) canis is mainly transmitted by direct or indirect contact with aborted fetuses and placenta. It's also known to be able to infect human, which likely results in providing veterinarians and companion animal owners for infectious risk. To develop diagnostic ELISA, we cloned and expressed rp1L gene of B. canis, which encodes the ribosomal protein L7/L12. Using this purified recombinant protein, indirect-ELISA (iELISA) was evaluated using 78 positive and 44 negative sera. The sensitivity and the specificity of iELISA were 94% and 89%, respectively. The results indicated that indirect-ELISA using recombinant ribosomal protein L7/L12 may be useful for diagnosis of canine brucellosis.

• 한국동물위생학회지
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• 제32권2호
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• pp.139-146
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• 2009

Bovine brucellosis is a zoonosis, long incubation period and chronic infectious disease, usually caused by Brucella abortus. This study was carried out to investigate the biotyping and biochemical characterization of B. abortus isolated from 208 farm 871 korean cattle and holstein diagnosed brucellosis by serological positive in Gyeongbuk province during the period from 2002 to 2006. B. abortus was isolated from 124 (14.2%) of 871 cattle, and isolated 110 (13.4%) of 820 Korean cattle and 14 (27.5%) of 51 holstein in breed. The uterus of korean cattle was isolated in 8 (17.8%) of 45 cattle and supramammary lymph none of holstein was isolated 11 (68.8%) of 16 cattle. 101 (12.5%) of 810 serological positive blood samples were isolated B. abortus. The isolation rate of B. abortus was correlated with antibody titers. The biochemical characterization of isolates was non-hemolytic, production of H$_2$S, oxidase-positive, catalase-positive, hydrolyzation of urea and growth of basic fuchsin dye medium. As a result, all of isolates was identified B. abortus bv 1. 124 isolates were susceptible to ampicillin, lincospectin, amikacin, gentamicin, kanamycin, neomycin, streptomycin, tetracycline, ciprofloxacin, norfloxacin and enrofloxacin.

• 한국임상수의학회지
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• 제17권2호
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• pp.321-326
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• 2000

전라남도 진도군 내에서 사육되는 진돗개에서 발생한 유산의 일부가 Brucell canis 에 의하여 유발된 것임을 확인하였다. 이 연구에서는 먼저 1997년 7월에서 1998년 6월 사이에 발생한 진돗개의 유산을 설문조사로 확인하였던 바 발생률이 18.5%에 달했다. 이어서 설문조사에 포함된 지역에서 사육되는 진돗개를 대상으로 신시한 혈청학적 조사에서는 25%가 양성을 보였으며, 수캐의 양성률은 암캐의 거의 두 배에 달했다. 다음으로 균 분리와 치료시험을 실시하기 위하여 유산 병력을 기치고 있으면서 혈청검사에서 양성을 나타낸 암캐 다섯 마리를 현지에서 구입하였다. 그 중 세 마리는 안락사 시킨 후 부검하여 자궁을 검사하고 균 분리를 시도한 바 세 미리 모두에서 B. canis가 분리되었다. 그러나 육안적 및 조직학적 변화 는 현저하지 않았다. 나머지 두 마리는 minocycline과 streptolmycin으로 치료하였는데, 치료를 끝낸 후 8주에 실시한 혈청검사에서 음성을 보였다. 이 연구의 결과는 개 부루셀라병이 진돗개를 사육하는 농가에 경제적 손실을 초래하고 있음을 나타내준다. 아울러 대부분의 진돗개가 방사되고 있으며, 그렇게 사육된 개들이 전국적으로 팔려나가고 있기 때문에 진돗개가 개 부루셀리병의 전파원으로 작용할 가능성을 지니고 있음을 나타내준다 천연기념물인 진돗개의 보 호와 농가의 소득 그리고 공중위생학적인 면에서 진품개의 부루셀라병에 관한 연구는 지속적으로 수행되어야 할 것으로 사료된다.