• Title/Summary/Keyword: Brown rice medium

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Changes in pasting properties and free fatty acids of different brown rice cultivar during storage

  • Choi, Induck;Kwak, Jieun;Yoon, Mi-Ra;Chun, Areum;Choi, Dong-Soo
    • Food Science and Preservation
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    • v.24 no.4
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    • pp.491-496
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    • 2017
  • Paddy rice is typically stored during postharvest until rice grain is processed into brown rice and milled rice by hulling and milling procedure, respectively. Recently, instead of storing paddy rice, storage of brown rice has been in the spotlight because it is more convenient and economically feasible. Different brown rice cultivars with varying amylose contents including waxy rice, medium-waxy rice, and non-glutinous rice were stored in room temperature storage for four months, and the changes in grain qualities of brown rice were evaluated. Amylose content significantly affected pasting properties in which rice cultivar with higher amylose content showed longer pasting time and higher peak viscosity. Storage also affected pasting viscosities, showing an increase in peak viscosity, but a decrease in breakdown viscosity. The changes in pasting viscosity during storage could be an important starch property for aged brown rice utilization. Waxy brown rice showed the weakest aging property in terms of free fatty acids (FFA) accumulation, whereas non-glutinous rice was more substantial grain quality against aging. The FFA values of two months storage were not significantly different from the initial FFA contents, suggesting that brown rice stored in room temperature for two months could be feasible for direct consumption of brown rice.

Coproduction of Enzymes and Beta-Glucan by Aspergillus oryzae Using Solid-State Fermentation of Brown Rice

  • Ji, Su Bin;Ra, Chae Hun
    • Journal of Microbiology and Biotechnology
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    • v.31 no.7
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    • pp.1028-1034
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    • 2021
  • The effect of medium composition on enzyme and β-glucan production by Aspergillus oryzae KCCM 12698 was investigated. Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation. To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid. Pretreatment of brown rice for 60 min prior to inoculation enhanced fungal biomass, while increasing the production of enzymes and β-glucan using solid-state fermentation. Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation. Therefore, solid-state fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.

Variation of Aflatoxin $B_1$ Production in Brown Rice Inoculated with Aspergillus parasiticus under Different Storage Conditions (현미의 저장조건에 따른 aflatoxin $B_1$ 생성의 변화)

  • 김종규
    • Journal of Food Hygiene and Safety
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    • v.13 no.1
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    • pp.47-52
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    • 1998
  • A rice cultivar (Japonica type), Cheong-cheong, was used to examine the ability as a substrate for aflatoxin production. Brown rice samples were inoculated with Aspergillus parasiticus, stored at various conditions, and observed the production of aflatoxin $B_1$ during storage. Enzyme-linked immunosorbent assay (ELISA) was performed to detect aflatoxin $B_1$ in the samples. A temperature of $28^{\circ}C$ favored the aflatoxin production in the samples. Remoisturizing brown rice to 15.8% encouraged the fungus to produce the aflatoxin significantly (p$B_1$ production in rice, and also indicated that other factors such as husking and storage periods were also risk determinants. This study provided evidence that rice could be an efficient medium for aflatoxin production.

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A New Selective Medium for Detecting Acidovorax avenae subsp. avenae in Rice Seeds

  • Song, Wan-Yeob;Kang, Mi-Hyung;Kim, Hyung-Moo
    • The Plant Pathology Journal
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    • v.16 no.4
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    • pp.236-241
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    • 2000
  • A selective agar medium was developed and tested for the isolation of Acidovorax avenae subsp. avenae, the causal bacterial pathogen of bacterial brown stripe, from rice seeds. The new selective agar medium, designated sorbitol pyroglutamic acid agar (SPA) medium, contained 0.5 g of $K_2$HPO$_4$, 3.0 g of Na$_2$HPO$_4$, 2.0 g of D-sorbitol, 0.2 g of L-pyroglutamic acid, 10.0 $m\ell$ of tween 80, 40.0 mg of victoria blue B, 15.0 g of agar, 150.0 mg of ampicillin and 25.0 mg of vancomycin per litter. Colonies of A. avenae subsp. avenae on SPA medium were smooth, round, convex, shiny, blue and 1.5-2.0 mm in diameter 4 days after incubation at 28$^{\circ}C$. Blue colored colony having dark blue zone was typical type of A. avenae subsp. avenae colonies on the medium. Mean recovery of 8 isolates of A. avenae subsp. avenae on the selective SPA medium was 95.8% in comparison to that on KB medium. The saprophytic bacteria were reduced to 97.9% on SPA medium compared to those on KB medium. Most of other rice seedborne bacteria as well as reported pathogenic bacteria were failed to grow on SPA medium. This medium was highly selective for recovering A. avenae subsp. avenae from rice seed samples, and it could be used to enhance the recovery of this bacterium from rice seed samples, which may be contaminated with large numbers of competing microorganisms.

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The Impact of Kinds of Dietary Grain and Dietary Lipid Level on the Glucose Metabolism and Antithrombogenic Capacity of Full Grown Obesity Induced Rats (식이 내 곡류 종류와 지방수준이 성장기 이후 비만유도 흰쥐의 당대사와 항혈전능에 미치는 영향)

  • Ok, Hyang-Mok;Sohn, Jung-Sook;Kim, Mi-Kyung
    • Journal of Nutrition and Health
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    • v.38 no.8
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    • pp.613-625
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    • 2005
  • This study was designed to evaluate impact of kinds of dietary grain and dietary lipid level on the glucose metabolism and antithrombogenic capacity in obesity induced rats. Total of 80 Sprague-Dawley male rats were raised for one month with control diet containing $50\%$ (w/w) well-milled rice powder and $20\%$(w/w) of dietary lipids. The rats were blocked into 8 groups and raised for two months with diets containing well-milled rice, brown rice, black rice, or glutinous barley powder and 8 or $20\%$(w/w) of dietary lipids. The contents of total dietary fiber in experimental grains were in following order; glutinous barley > black rice > brown rice > well-milled rice. Weekly food intake were lower in glutinous barley group among all experimental groups. Body weight gain was high in high level of fat groups ($50\%$w/w) than medium level of fat groups ($8\%$ w/w). Plasma glucose concentration was not different significantly in each groups. But brown rice group was a little lower than others. Plasma insulin concentration was lower in black rice and glutinous barley group than rice group. Plasma glucagon concentration did not differ significantly among all experimental groups. Hexokinase activities in skeletal muscle are different significantly according to level of dietary fat and grain variety factors. Brown rice group was significantly highest among all experimental groups in hexokinase activity. Plasma $TXB_2$ concentrations in black rice and glutinous barley groups were lower as compared to rice and brown rice groups. Plasma 6-keto-$PGF_{1\alpha}$ concentrations in glutinous barley group was higher as compared to others. In conclusion brown rice has a little lowering effect glucose concentration. Black rice and glutinous barley intakes enhance antithromboenic capacity. It is suggested that the intakes of mixed gains are recommend.

Current Status of Bacterial Brown Stripe of Rice Caused by Acidovorax avenae subsp. avenae (Acidovorax avenae subsp. avenae에 의한 세균성줄무늬병의 연구동향)

  • 송완엽
    • Plant Disease and Agriculture
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    • v.5 no.2
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    • pp.69-76
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    • 1999
  • Acidovorax avenae subsp. avenae is the causal pathogen of several hosts including oats corn foxtail millet wheatgrass sugarcane and rice. The pathogen is a seedborne pathogen of rice and known to occur widely in rice growing countries. The pathogen cause inhibition of germination brown stripe on the leaf curling of the leaf sheath and abnormal elongation of the mesocotyl of irce. Bacterial colonies grow slowly and are convex circular and creamy with tan to brown center. The causal baterium is Gram-negative and rod shape with a single polar flagellum Nonfluorescence poly-$\beta$-hydroxybutyrate accumulation and precipitate formation around the colony on the medium are useful in the differentiation of this bacterium from other subspecies of A. avenae as well as nonfluorescent bacteria pathogenic to rice. This bacterium has belonged to the genus of Psdeudomonas but recently was transferred to the new genus Acidovorax on the basis of bacteriological and molecular biological data. However the difference of biochemical characteristics protein profile of the cell and host range among strains should be more clarified. To develop an effective control strategy for this disease understanding of detailed life cycle of the disease ritical environmental factors affecting disease development on each host and relationship to grain discoloration of rice are prerequisite. Although the affected area has been world-widely reported there is on recent progress on the understanding of the bacteriological and ecological characteristics of the causal bacterium and control means of the disease.

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Storage properties of brown rice cultivar as affected by milling degree and storage conditions

  • Choi, Induck;Yoon, Mi-Ra;Kwak, Jieun;Chun, Areum;Jeon, Yong-Hee;Jeong, Tae-Wook;Kim, Sun-Lim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.262-262
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    • 2017
  • Brown rice contains a variety of nutritional and bio-functional components compared to milled rice. However, low consumer preference for steamed brown rice and short shelf life has been implicated as deterrents to the direct consumption of brown rice. This study was undertaken to determine the effect of degree of milling (DOM) on pasting and aging properties of brown rice cultivar. Short-grain (Japonica type) brown rice cv. Boseogchal (BSC), Baegjinju (BJJ), and Hiami (HIAM) were evaluated every month during 4 months storage. Rice cultivar were categorized into waxy (BSC), medium-waxy (BJJ), and non-waxy (HIAM) of which amylose contents were 6.81%, 10.06%, and 16.69~16.72%, respectively. Peak viscosity was the highest in BJJ followed by HIAM, and BSC, showing peak viscosity was lower at low temperature storage. At storage at room temperature from 60 to 120 days, the FFA content significantly increased in the rice samples with DOM1% followed by 3% and 5%, while not significant increase in DOM0% brown rice: at 120 days, the FFA ranged in 28.35~41.48, 111.99~130.06, 76.87~91.99, and 47.13~62.37 mg KOH/100g for DOM 0%, 1%, 3% and 5%, respectively. It indicated that the fat rancidity was the least in the order of DOM 0%, 5%, 3% and 1%. Storage at low temperature could reduce fat hydrolysis with lower FFA release, but rice grain with 1% MD also released more FFA compared to the other samples. It could be assumed that the most outer bran layer, approximately 1~3%, could provide a strong protection from fat rancidity. Pasting viscosity of rice samples also increased with increasing DOM rate. Results indicated that DOM was the most significant factor for lipid rancidity during storage, resulting in high FFA formation. It suggested that brown rice with DOM 0% and/or more than DOM 5% could provide the best shelf life.

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Variation in Leaf Mechanical Damage by Typhoon among Rice Cultivars: Effects on Yield and Rice Quality (태풍에 의한 벼 잎 파열의 품종간 차이 및 잎 파열이 수량과 미질에 미치는 영향)

  • 홍광표;김영광;정완규;손길만;송근우
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.3
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    • pp.199-206
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    • 2004
  • Typhoon "MAEMI", happened in September 2003, hit the great damage to Gyeongnam province area. Especially, rice plant was lodged or severe leaf damage was caused. This study was conducted to find out the extents of leaf damage among different rice cultivars, and to evaluate rice yield and eating quality due to leaf damage after typhoon. Rice cultivars torn off over half of the flag leaf length (FLL) were one medium-late maturing cultivar (Donginl), medium maturing cultivar (Yeonganbyeo), eight early maturing cultivars (Samcheonbyeo, Jounghwnbyeo, Munjangbyeo, Taebongbyeo, Odaebyeo, Samhaegbyeo, Sobaegbyeo, Sinunbongbyeo), two long-il type cultivars (Samgangbyeo, Namcheonbyeo), and three special use cultivars (Heukseonchalbyeo, Jinbuchalbyeo, Yangjobyeo). Cultivars torn off below 1/10 FLL were eight medium-late maturing cultivars (Chucheongbyeo, Daecheongbyeo, Saechu cheongbyeo, Donganbyeo, Daeyabyeo, Hwamyeongbyeo, 방eongsanbyeo, Dongjinbyeo) and two medium maturing cultivays (Donghaebyeo, Gumobyeo2). The rest cultivars were tore off by 1/10∼1/2 ELL. In yield components, the longer was flag leaf damage, the lower was ripened grain ratio, grain weight and brown/rough rice ratio, which was severly impacted to late than to ordinary season cultivation. However, rice yield did not decrease up to tearing by 1/10 ELL. Head rice ratio decreased from flag leaf tearing over 1/10 ELL in late season cultivation. The longer was flag leaf damage, the lower was eating quality, which could not show significantly different.

Growth Characteristics of Paecilomyces tenuipes by the Passage in Liquid Media (액체배지에서 눈꽃동충하초균(Paecilomyces tenuipes)의 계대증식에 따른 증식 특성)

  • Oh, Jeong-Mi;Lee, Jae-Kyung;Koo, Hyun-Na;Woo, Soo-Dong
    • Korean journal of applied entomology
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    • v.47 no.2
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    • pp.169-174
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    • 2008
  • The growth characteristics of Paecilomyces tenuipes according to the passage in the two kind of liquid media were investigated by comparing the mycelium and conidium formation degrees. The potato dextrose broth medium and the silkworm larvae medium containing the silkworm powder were used as the liquid media, and the potato dextrose agar medium and the brown rice medium containing the powder of silkworm pupa were used as the solid media. The conidium formation degree in liquid media differed by the passages but that in solid media was not. This suggested that the passage in liquid media did not affect significantly the conidium formation in solid media. When the brown rice media were inoculated with the concentration of $1{\times}10^{10}$ conidia/ml, $1{\times}10^8$ conidia/ml and $1{\times}10^6$ conidia/ml, respectively, the conidium formation degrees were similar. This indicated that the optimal inoculation concentration of conidium to the brown rice media is $1{\times}10^6$ conidia/ml.

Mycelial Growth Using the Natural Product and Angiotensin Converting Enzyme Inhibition Activity of Pleurotus eryngii (천연물을 이용한 큰느타리 균사배양 및 Angiotensin Converting Enzyme 저해활성)

  • Kang, Tae-Su;Jeong, Heon-Sang;Lee, Myong-Yul;Park, Hee-Joeng;Jho, Taek-Sang;Ji, Seung-Taek;Shin, Myung-Keun
    • The Korean Journal of Mycology
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    • v.31 no.3
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    • pp.175-180
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    • 2003
  • To develop the health/functional food materials, we investigated the cultural condition of mycelial growth on the solid state fermentation using the brown rise, Acanthopanax sp. and Artemisia sp., and also evaluated inhibitory activity of angiotensin converting enzyme (ACE) of hot water extracts from cultured media of Pleurotus eryngii. As the amount of Acanthopanax nnd Artemisia In the cultural media increased, the mycelial growth rate decreased. Especially, addition of Aeantopanax showed marked effect than Artemisia. Moisture contents in three kinds of cultured media were in the range of $10.9{\sim}12.0%$. Crude protein fat and crude fiber content were the highest value in cultured brown rice medium, whereas the mineral contents (Ca, K and P) were higher in the Acanthopanax supplemented (5%) medium than the other media, The extraction yield of the Artemisia supplemented (5%) medium was the highest value of 4.80%, and the pH of hot water extract from cultured brown rice medium showed the lowest value of 6.1. Lightness (L) values in three kinds of extracts from cultured media were in the range of $85.8{\sim}87.1$. Redness (a) value was the highest In the brown rice and Acanthopanax supplemented media, however cultured Artemisia supplemented medium showed the highest value in yellowness (b). In comparison of sugar components analyzed by the thin layer chromatography with three kinds of samples, two spots were detected to be glucose and maltose, respectively. The ACE inhibitory activity of hot water extract from the cultured Acanthopanax supplemented medium showed the highest value at the concentration of $0.2{\sim}1.0\;mg/ml$. These results suggest that the Pleurotus eryngii grew in natural media using brown rice and Acanthopanax can be supplemented to the brown rice medium to enhance its ACE inhibitory activity as health/functional food materials.