• Journal of Animal Science and Technology
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• v.59 no.10
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• pp.23.1-23.11
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• 2017

Background: Although soybean meal (SBM) is excellent source of protein in diets for poultry, it is sometimes inaccessible, costly and fluctuates in supply. The SBM can partially be replaced by full-fat SBM, but the meals prepared from raw full-fat soybean contain antinutritional factors. To avoid the risk of antinutritional factors, heat treatment is always advisable, but either excessive or under heating the soybean could negatively affect the quality. However, the potential for further improvement of SBM by supplementing with microbial enzymes has been suggested by many researchers. The objective of this study was to evaluate the performance and ileal nutrient digestibility of birds fed on diets containing raw soybeans and supplemented with microbial protease. Methods: A $3{\times}2$ factorial, involving 3 levels of raw full-fat soybean (RFFS; 0, 45 or 75 g/kg of diet) and 2 levels of protease (0 or 15,000 PROT/kg) was used. The birds were raised in a climate-controlled room. A nitrogen-free diet was also offered to a reference group from day 19 to 24 to determine protein and amino acid flow at the terminal ileum and calculate the standardized ileal digestibility of nutrients. On days 10, 24 and 35, body weight and feed leftover were recorded to calculate the body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR). On day 24, samples of ileal digesta were collected at least from two birds per replicate. Results: When RFFS was increased from 0 to 75 g/kg of diet, the content of trypsin inhibitors was increased from 1747 to 10,193 trypsin inhibitors unit (TIU)/g of diets, and feed consumption of birds was also reduced (P < 0.05). Increasing RFFS level reduced the BWG from hatch 0 to 10 d (P < 0.01) and hatch to 24 d (P < 0.05). The BWG of birds from hatch to 35 was not significantly (P = 0.07) affected. Feed intake was also reduced (P < 0.05) during 0 to 35 d. However, protease supplementation improved (P < 0.05) the BWG and FCR during 0 to 24 d. Rising levels of RFFS increased the weight of pancreas (P < 0.001) and small intestine (P < 0.001) at day 24. Except for methionine, apparent and the corresponding standardized ileal digestibility of CP and AA were reduced (P < 0.01) by increasing levels of RFFS in diets. Conclusion: This study showed that some commercial SBM could be replaced by RFFS in broiler diets, without markedly compromising productivity. The AID and SID of CP and lysine were slightly improved by dietary supplementation of microbial protease.

• Korean Journal of Poultry Science
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• v.30 no.1
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• pp.49-54
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• 2003

This study was carried out to investigate the effects of the inoculated diet with Paecilomyces japonica on weight gain, feed efficiency and cordycepin contents in the breast and thigh meats of broilers. One hundred and twenty broiler chicks were divided into four dietary groups ; 1) control (basal diet), 2) T1 (substituted with 5% inoculated diet), 3) T2 (substituted with 10% inoculated diet) 4) T3 (substituted with 20% inoculated diet) during 4 weeks. Cordycepin contents of inoculated diet with Pacilomyces japonica was significantly higher than before inoculation (P<0.05). Body weight gain and feed efficiency were highest in T1 group when fed starter diet (P<0.05) but these were lowest in T3 group fed when fed finished diet (P<0.05). Body weight gain and feed efficiency were the lowest in T2 group for overall period than other groups. Cordycepin contents of breast and thigh meats were significantly higher in Pacilomyces japonica groups than control at 5 weeks of age (P<0.05). However, it was similar among the T1, T2 and T3 groups. These results indicated that body weight gain and feed efficiency tended to increase in birds fed replaced 5% inoculation diet Cordycepin contents of breast and thigh meats would be increased significantly in birds fed inoculated diet (P<0.05).

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.12
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• pp.1732-1741
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• 2013

Two experiments were conducted to determine physical and chemical properties of vermicelli waste (VW) and effect of VW inclusion levels on growth performance of broilers. In experiment 1, VW samples were randomly collected from vermicelli industry in Thailand to analyze nutritional composition. Vermicelli waste contained 9.96% moisture, 12.06% CP, 32.30% crude fiber (CF), and 0.57% ether extract (EE), as DM basis. The ratio of insoluble:soluble non-starch polysaccharide (NSP) was 43.4:8.9. A total of 120 chicks (6 pens per treatment and 10 chicks per pen) were fed a corn-soybean meal-based diet or 20% VW substituted diet to determine the apparent metabolizable energy corrected for nitrogen retention ($AME_n$) of VW. The $AME_n$ of VW was $1,844.7{\pm}130.71$ kcal/kg. In experiment 2, a total of 1,200 chicks were randomly allotted to 1 of 4 dietary treatments for 42-d growth assay. There were 300 chicks with 6 pens per treatment and 50 chicks per pen. The dietary treatments contained 0%, 5%, 10%, or 15% VW, respectively. All diets were formulated to be isocaloric and isonitrogenous. From 0 to 18 d of age chicks fed VW diets had higher (p<0.001) feed conversion ratio (FCR) compared with those fed the control diet. No difference was observed during grower and finisher phase (19 to 42 d). Chicks fed VW diets had lower relative weight of abdominal fat (p<0.001) but higher relative weight of gizzard (p<0.05) than those of chicks fed the control diet. Increasing VW inclusion levels increased ileal digesta viscosity (p<0.05) and intestinal villus height of chicks (p<0.001). For apparent total tract digestibility assay, there were 4 metabolic cages of 6 chicks that were fed experimental treatment diets (the same as in the growth assay) in a 10-d total excreta collection. Increasing VW inclusion levels linearly decreased (p<0.05) apparent total tract digestibility of DM and CF.

• Korean Journal of Poultry Science
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• v.40 no.1
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• pp.41-47
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• 2013

This work was carried out to investigate performance and carcass yield of large-type broiler females at different stocking densities. Chicks (n = 492; 1-day-old; female; Arbor Acre broiler) were reared for six weeks (starter, 0~1 wk; earlier, 1~3 wk; finisher, 3~6 wk) and randomly divided into 3 treatments (4 replications/treatment, 36, 41 or 46 birds/replication). Treatments were T1 (10.9 birds/$m^2$), T2 (12.4 birds/$m^2$) and T3 (13.9 birds/$m^2$) by the stocking densities. Research indexes were livability, body weight, body weight gain, feed intake, feed conversion ratio, production efficiency factor, carcass yield and primal cuts ratio (wing, leg, back, breast and neck). Livability (%) was 90% or more for all treatments, but there was no significant difference on weekly livability (%). Body weight of T3 treatment were the lowest at 3 wk and body weight gain, feed intake, and feed conversion ratio were the lowest at 2~3 wk (P<0.05). Production efficiency factors of T1, T2 and T3 were 331.0, 340.8 and 336.0, respectively, and there was no significant difference among treatments. Leg meat ratio of T2 treatment was higher compared to other treatments at the age of 4 wk (P<0.05). Carcass yield of T1 treatment was the highest as 76.2% at the age of 5 wk (P<0.05). However, there was no significant difference on carcass ratio and partial meat ratio among treatments at the age of 6 wk. This result may provide the standard data of different stocking densities for heavy female broilers.

• Korean Journal of Poultry Science
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• v.42 no.1
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• pp.33-40
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• 2015

This study was conducted to investigate the effects of dietary quercetin on growth performance, blood biochemical parameters, immunoglobulin, and blood antioxidant activity in broiler chickens. Three hundred twenty one-day old Ross broilers were divided 8 treatments (C(-), basal diet; C(+), basal diet with antibiotics; vitamin E 20 IU; vitamin E 200 IU; quercetin 20 ppm; quercetin 200 ppm; methoxylated quercetin 20 ppm; methoxylated quercetin 200 ppm) with 4 replicates and 10 birds per replicate. Birds were reared for 35 days and their feed intake and weight gain were measured weekly. At 35d, eight birds of average weight from each replicate were selected for blood collection and analysis. Weight gain of birds in the groups fed quercetin was higher when compare to NC but there was no significant difference. In the serum, creatinine, BUN and AST in quercetin groups significantly decreased compared to those of control (NC and PC) (P<0.05). The contents of IgA and IgM were significantly lower in quercetin groups than those of NC (P<0.05). SOD like activity and MDA content tended to decrease in quercetin groups, however, there was no significant difference among treatments. In conclusion, supplemental quercetin to poultry diet could be positive aspect on performance and blood metabolites. Optimum adding levels was more than 20 ppm.

• Korean Journal of Poultry Science
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• v.37 no.4
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• pp.433-438
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• 2010

This study investigated the effects of dietary supplementation of the fermented by products garlic and onion on growth performance, blood composition and cecal microflora in broiler chickens. A total four hundred eighty, day old broiler chickens (Ross) were randomly divided into four groups with four replicates of thirty birds each. The treatment groups were negative group (NC, antibiotic-free diet), positive group (PC, basal diet with 0.05% and 0.03% anticoccidials), fermented of onion by product 1.0% group (T1) and fermented of onion by product 1.0% group (T2). The body weight of broilers fed the diets containing fermented by products garlic was higher than the other treatments during overall period. No significant difference were observed on serum chemical composition and blood corpuscle. In the cecal microflira of broiler, the population of the Lactic acid bacteria was showed the higher in chicken fed diets supplemented with fermented of garlic group than other groups (P<0.05). These results suggest the possibility that fermented of garlic and onion by product could be used as the alternative of antibiotics growth promotor of broiler chickens.

• Korean Journal of Organic Agriculture
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• v.18 no.3
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• pp.347-361
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• 2010

This study was carried out to investigate the effects of wormwood (Artemisia sp.) addition on the growth performance, nutrients utilization and abdominal fat deposition of broiler chickens. Two hundred twenty five and two-day old Arboracre strain male commercial broiler chicks were distributed to 5 treatments with wormwood supplementation levels; C(O%), $T_1$ (1%), $T_2$ (3%), $T_3$ (5%), and $T_4$ (10%) and with 3 replications each with 5 birds for five weeks. Body weight gain during the experiment was improved in $T_1$ (1723.0g) compared with that of $T_3$ (1557.7g) and $T_4$ (1450.7g) (P<0.05). Feed intake was significantly (P<0.05) increased as the levels of wormwood addition increased (C: 2653.8g, T1: 2852.0, $T_2$: 2900.3, $T_3$: 2900.7g, T4: 2954.7g). Feed conversion rate (feed/gain) was significantly (P<0.05) increased as the levels of wormwood addition increased (C: 1.55, $T_1$: 1.66, $T_2$: 1.70, $T_3$: 1.86, $T_4$: 2.04). The days reaching to 2.0kg of body weight were expected to be 43.2 days in control, whereas those of group T1 were reduced to 42.6 days by 0.6 day from control. Nutrient utilization and abdominal fat deposition in the experiment were significantly decreased (P<0.05) and small intestine contents in the broilers was significantly increased (P<0.05) as the levels of wormwood addition increased. Therefore, although there was no significant improvement for the performance of broiler chickens with the dietary supplementation of wormwood meal, less than 1% addition of wormwood to broiler diets might have beneficial for human health by reducing the abdominal fat deposition of the broiler chicknens.

• Journal of Animal Science and Technology
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• v.44 no.3
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• pp.289-296
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• 2002

A broiler experiment was conducted to investigate the effect of supplementing yeast culture (Saccharomyces cerevisiae, Pichia pastoris) on the growth performance, small intestinal microflora and immune response in broiler chickens. One thousand hatched broiler chickens(Ross$^{(R)}$) were assigned to 6 treatments: control (basal diet), CTC; chlorotetracycline 100ppm, YC-SC; yeast culture(Saccharomyces cerevisiae) 0.3%, YC-PP; yeast culture(Pichia pastoris) 0.3%, RPPC-0.1; refined Pichia pastoris culture 0.1%, RPPC-0.3; refined Pichia pastoris culture 0.3%. There were no significant differences in growth, feed intake, feed efficiency and mortality among the treatments. However, chickens fed diets with yeast cultures showed numerically higher weight gain than those fed the control diets. Supplementation of yeast cultures and CTC improved feed efficiency and decreased mortality compared to control. Nutrient digestibilities were not affected by the dietary treatments. Total number of Lactobacilli in small intestine was higher while that of Cl. perfringens was lower with yeast culture treatments than control. Small intestine E. coli population of RPPC-0.3 treatment was significantly lower than that of the control. The serum IgG concentration tended to be higher in broilers fed yeast cultures than those fed the control and CTC diet. In conclusion, the supplementation of yeast culture products showed, although not significant but, numerical advantages in productivity and profile of microbial flora and serum IgG compared to the control and CTC supplementation.

• Korean Journal of Poultry Science
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• v.34 no.3
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• pp.231-236
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• 2007

A feeding trial was conducted to study the effects of dietary supplement of fermented clay mineral (FCM) on the growth performance and immune stimulation in broiler chickens. A total of 36,800 broilers, (of) Ross strain-208, were randomly allotted into 2 experimental (diet) groups. Control group was fed the basal diet (corn-soybean meal based), and treatment group was fed the natural clay mineral that was fermented with aboriginal microorganisms at $37^{\circ}C$ for 72 h. Feed and water were provided ad libitum throughout the experiment for 5 weeks of experimental feeding period. Daily weight gain of treatment group fed 0.3% fermented clay mineral was significantly increased in comparison with control group. Feed intake of treatment group was significantly increased 21.4% (p<0.05), and feed efficiency was also increased 2.7% compared to control group. Especially, growth rate and production index of treatment group were significantly higher as much as 4.4% and 4.8%, respectively, than those of control group (p<0.05). By the feeding of dietary 0.3 % FCM, the antibody productions against Newcastle Disease and Infectious Bursal Disease antigens were shown to increase 13.8 % and 15.7 %, respectively, more than control group chickens. From these results, it appears that the supply of fermented clay mineral at 0.3 % level in the broiler chicken diet could enhance the growth performance and immune stimulation of broiler chickens.

• Korean Journal of Poultry Science
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• v.41 no.2
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• pp.143-149
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• 2014

This study was conducted to investigate the effects of dietary supplementation of the mixture of probiotics (MP) on growth performance, size of small intestine, cecal microflora and ammonia concentrations in broiler chicks. A total of 700, one-day-old male broiler chicks were randomly allotted to four treatments with seven replications having 25 birds per pen. The birds were fed one of the four experimental diets; containing no antibiotics nor MP (negative control, NC), containing antibiotics without MP(positive control, PC), negative control with MP 0.1% and negative control with MP 0.2% for 5 weeks. During overall experiment, birds in PC and MP treatments had higher final BW and daily BW gains than birds in NC treatments; however, the significance was not identified. The feed conversion ratio of the chicks fed the diet containing MP was significantly improved as compared to those fed the NC diets. The weights of jejunum were increased by the MP (p<0.05), but weights of duodenum, ileum and length of small intestine were similar among the groups. Birds in PC treatment showed lower populations of total microbes and lactic acid bacteria than other groups (p<0.05), cecal ammonia concentrations of the chicks fed the diet containing MP were significantly decreased as compared to those of NC and PC (p<0.01). In conclusion, MP added to the broiler diets improved the feed conversion rate and reduced cecal ammonia concentration.