• Korean Journal of Plant Resources
• /
• v.26 no.1
• /
• pp.26-35
• /
• 2013

This study was conducted to select superior provenances of Scots pine (Pinus sylvestris L.) well adapted to Korean environment for timber production. In 1976, twenty-two provenances of Scots pine were introduced from Sweden and the seeds were sown in seed beds in March. After one year, the seedlings were transplanted to nursery beds. The resulting 1-1 seedlings of 22 provenances were planted at Whaseong in 1978. Randomized complete block design with 3 replications were used for test plantation. Each provenance was planted with 20-tree row plot in each block and at a spacing of $1.8{\times}1.8m$. The growth performance of each provenance was monitored up to 33-years after planting. There were significant differences among provenances in volume growth. F3001 provenance showed the best volume growth of 33-years after planting ($0.160m^3$), which was 2.2 times greater than that of the lowest provenance W2027 ($0.072m^3$). The ranking of provenances was stabilized after 14 years. Comparing to reference tree species, Japanese red pine (Pinus densiflora), all Scots pine provenances showed poor growth performance. In other words, volume growth of Japanese red pine at age 28 and 33 were 2.1 and 3.3 times greater than that of Scots pine, respectively. Moreover, survival rate of Scots pine was lower than that of Japanese red pine. Based on these results, it was suggested that Scots pine was not suitable to Korean environments. The cause of maladaptation of Scots pine and the implications of introduction breeding were discussed.

• Korean Journal of Medicinal Crop Science
• /
• v.15 no.5
• /
• pp.339-345
• /
• 2007

Field performance and morphological characterization was conducted on seven transgenic lines of Codonopsis lanceolata expressing ${\gamma}-TMT$ gene. The shoots were obtained from leaf explants after co-cultivation with Agrobacterium tume-faciens strain LBA 4404 harboring a binary vector pYBI 121 that carried genes encoding ${\gamma}-Tocopherol$ methyltransferase gene (${\gamma}-TMT$) and a neomycin phosphotransferase II gene (npt II) for kanamycin resistance. The transgenic plants were transferred to a green house for acclimation. Integration of T-DNA into the $T_0\;and\;T_1$ generation of transgenic Codonopsis lanceolata genome was confirmed by the polymerase chain reaction and southern blot analysis. The progenies of transgenic plants showed phenotypic differences within the different lines and with relative to control plants. When grown in field, the transgenic plants in general exhibited increased fertility, significant improvement in the shoot weight, root weight, shoot height and rachis length with relation to the control plants. However, all seven independently derived transgenic lines produced normal flower with respect to its shape, size, color and seeds number at its maturity. Indicating that the addition of a selectable marker gene in the plant genome does not effect on seed germination and agronomic performance of transgenic Codonopsis lanceolata. $T_1$ progenies of these plants were obtained and evaluated together with control plant in a field experiment. Overall, the agronomic performance of $T_1$ progenies of transgenic Codonopsis lanceolata showed superior to that of the seed derived non-transgenic plant. In this study, we report on the morphological variation and agronomic performance of transgenic Codonopsis lanceolata developed by Agrobacterium transformation.

• Korean Journal of Agricultural Science
• /
• v.46 no.2
• /
• pp.351-359
• /
• 2019

The purpose of this study was to compare the growth performance, nutrient digestibility, fecal microflora, blood profiles and meat quality of broilers, Korean native chickens and white semi broilers under identical feeding conditions. Sixty 1-d chicks of each type were randomly placed into 12 pens per cage (5 chicks per cage) and fed commercial diets for 28, 49, and 28 days, respectively. The broilers showed a significantly higher (p < 0.05) growth performance from 0 to 4 weeks compared with the other types. Korean native chickens had a significantly higher (p < 0.05) digestibility of dry matter and nitrogen than that of the other species. The blood glucose was significantly higher in the Korean native chickens compared to the other species, and the blood cholesterol was significantly lower. The Lactobacillus content in Korean native chickens was significantly higher (p < 0.05) compared to the other species, and the E. coli content in the broilers was significantly higher (p < 0.05) than in the Korean native chickens and white semi broilers. In terms of meat quality, the breast meat of the broilers had a high water content and pH level. The breast meat of the white semi broilers had a significantly lower (p < 0.05) shear force than that of the other species. In conclusion, there was an interspecific physiological difference due to the age and body weight of the chickens. The broilers had a higher growth performance and meat quality compared to the Korean native chickens and white semi broilers.

• Korean Journal of Poultry Science
• /
• v.41 no.3
• /
• pp.191-196
• /
• 2014

The identification and utilization of potential candidate genes with significant effects on economically important traits have become increasingly important in poultry breeding programs. The ovocalyxin-32 (OCX-32) gene is located chromosome 9 in chicken, plays an important role in eggshell formation. This study was performed to assess the association between single nucleotide polymorphisms (SNPs) of OCX-32 gene and egg production traits in the Korean native chicken. Four Korean native chicken population (n = 181; including 46 females of Ogol, 46 females of white, 43 females of gray and 46 females of black) were used to analyze two SNPs (c.494A>C and c.267T>G) in the OCX-32 gene by PCR-RFLP (Polymerase Chain Reaction-Restricted Fragment Length Polymorphism). We measured egg production traits of age at first egg, first egg weight, egg production ratio and egg weight. The SNP c.494A>C was significantly associated with egg production ratio in Korean Ogol chickens (p<0.001) and egg weight in Korean white chickens (p<0.05). SNP c.267T>C was significantly associated with egg weight in Korean Ogol chickens (p<0.05). But there was no significant association in Korean gray and black chickens. Results suggest the possibility of using molecular markers in OCX-32 gene as a tool for performance and egg production traits in Korean native chicken breeding program.

• Horticultural Science & Technology
• /
• v.30 no.6
• /
• pp.743-750
• /
• 2012

A total of 95 broccoli (Brassica oleracea L. var. italica) accessions were evaluated for the identification of desulfo-glucosinolates and their content variation in the flower head using ultra performance liquid chromatography, to select the potentially functional broccoli breeding lines. The six individual desulfo-glucosinolates, including progoitrin, glucoraphanin, sinigrin, gluconapin, glucobrassicanapin, and glucobrassicin, were commonly identified, based on the chromatogram peak comparison with those of the nine individual glucosinolate standards. The total glucosinolate contents varied from 4.2 to $29.0{\mu}mol{\cdot}g^{-1}$ DW and the glucoraphanin (1.6 to $13.9{\mu}mol{\cdot}g^{-1}$ DW) was confirmed as a major constituent in the total glucosinolate profile among the six identified individual glucosinolate species, whereas the progoitrin, which was only detected in 13 accessions, showed accession-specific variation and negative correlation with glucoraphanin content. It was also revealed that the four major glucosinolates, such as glucobrassicanapin, glucoraphanin, glucobrassicin, and gluconapin, affected major content variation and showed higher positive inter-correlation. These results might be used for the selection of potential breeding materials as functional broccoli germplasm through the further evaluation on the stability and reproducibility of glucosinolate profile depending on environmental factors or cultural managements using the selected accessions.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.47 no.3
• /
• pp.234-240
• /
• 2014

We investigated the effects of feeding rate on the growth performance, blood components, and histology of growing olive flounder Paralichthys olivaceus. We determined the optimum feeding rate (initial fish mean weight of $240{\pm}10.9$ g) at the optimum water temperature. Two replicated groups of fish were fed a commercial diet at rates of 0%, 0.5%, 0.75%, and 1.0% body weight (BW) per day, and to satiation. Feeding trial was conducted using a flow-through system with 10 1.2-metric ton aquaria receiving filtered seawater at $19-21^{\circ}C$ for three weeks. Weight gain (WG) for fish fed to satiation was significantly higher than that of unfed fish and fish fed at 0.5% and 0.75% BW per day. The WG of fish fed at 1.0% BW per day was significantly higher than that of unfed fish and of fish fed at 0.5% BW per day. However, there were no significant differences in WG between fish fed at 0.5% BW per day and those fed at 0.75% BW per day, between fish fed at 0.75% BW per day and those fed at 1.0% BW per day, and between fish fed at 1.0% BW per day and those fed to satiation. The specific growth rates of fish fed at 1.0% BW per day and those fed to satiation were significantly higher than those of unfed fish and of fish fed at 0.5% BW per day. Broken-line regression analysis of weight gain showed that the optimum feeding rate of olive flounder weighing 240 g was 1.09% BW per day at the optimum water temperature.

• Korean Journal of Agricultural Science
• /
• v.42 no.4
• /
• pp.361-368
• /
• 2015

This study was to investigate the effect of high energy diet on characteristics of Hanwoo steers distributed by estimated breeding value (EBV). The aim of this experiment was to determine the effect of high energy diet on the high and low beef group distributed by EBV for quality grades. We hypothesized that high energy diet is able to increase quality traits in high EBV groups when fed a high energy diet. A $2{\times}2$ factorial arrangement (High energy, control vs high EBV, low EBV) in a completely random design was used to feed 26 Hanwoo steers. Blood was drawn from each steers from 11 to 28 months. ADG and feed efficiency were not different between high energy and control diet (P>0.05). The level of DMI was greater at calf and early fattening diet in low EBV groups (P<0.05). Serum glucose and tryglyceride conecntrations were increased (P<0.05) by high EBV group from 22 to 28 month old. Serum NEFA concentration were plateau at 24 months at high EBV group and steady reduced by high energy diet (P<0.05). This data indicated that high energy diets increased serum glucose and triglyceride concentrations of high EBV steers at final fattening period.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.10
• /
• pp.181-186
• /
• 2020

This study examined the artificial fertilization efficiency of crossbred goats from a farmhouse using frozen semen. Electrostimulation was used to ejaculate and collect semen to assess the artificial fertilization efficiency of crossbred goats. The sperm concentration, vitality, and vitality after melting were investigated. The sperm volume was within 2.5~3 ml, and the concentration was 21~25 × 10⁸/ml for each male crossbred goat. The melted semen had high vitality (≥90%). An IDEXX Rapid Visual Pregnancy Test kit was used for an earlier diagnosis of the pregnancy and to determine the pregnancy rate of fertilization using frozen-thawed semen. The reproductive performance of the artificially fertilized crossbred goats had the highest delivery rate (68%) from Farm C and the lowest delivery rate (45%) from farm A. The delivery rate through artificial fertilization was equal to the fertilization rate according to early pregnancy diagnostic kits. The artificial insemination efficiency was 45~68%. These findings can be used as the basis for improvement and breeding goats in goat farms and livestock research institutes.

• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.9
• /
• pp.1189-1192
• /
• 2000

The experiment was conducted on 69 cows to identify the quantitative variations of some economic traits of five genetic groups as $Local{\times}Friesian $ $F_1$, $Local{\times}Sahiwal$ $F_1$, Local (indigenous zebu type), $Local{\times}Sindhi $ $F_1$, and $Local{\times}Hariana$ $F_1$. The traits studied were age at weaning, age at first heat, age at first conception, services per conception, daily milk yield, lactation length, lactation yield and post partum heat period. The records on milking and reproduction performances of cows and heifers were obtained from farm register and by interviewing the farmers. It was observed that the lowest age at weaning, age at first heat and age at first conception were $5.37{\pm}0.24$, $27.17{\pm}1.72$ and $27.83{\pm}1.82$ months respectively in $Local{\times}Hariana$ $F_1$. Services per conception were lowest in $Local{\times}Sahiwal$ $F_1$ $(1.08{\pm}0.18)$ although not significantly (p>0.05) affected by farms, genetic groups and $farm{\times}genetic$ groups interaction. Average daily milk yield was highest in $Local{\times}Friesian$ $F_1$ $(5.81 {\pm}0.40 kg)$. Lactation length and lactation yield were highest in $Local{\times}Sahiwal$ $F_1$ ($299.38{\times}9.74$ days and $1863.00{\pm}141.00kg $ respectively). Average post partum heat period was lowest in $Local{\times}Sindhi$ $F_1$ ($3.19{\pm}0.38$ months). Least squares ANOVA showed that farm had significant (p<0.001) effect on age at weaning, age at first heat, age at first conception and post partum heat period. There was insignificant (p>0.05) effect of farm on services per conception, daily milk yield, lactation length and lactation yield, whereas genetic groups had a significant effect for all the traits under review except services per conception. $Farm{\times}genetic$ groups interaction was insignificant for all of the traits under consideration except age at weaning.

• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.3
• /
• pp.320-327
• /
• 2017

Objective: The objective of this research was to estimate genetic correlations between number of piglets born alive in the first parity (NBA1), litter birth weight in the first parity (LTBW1), number of piglets weaned in the first parity (NPW1), litter weaning weight in the first parity (LTWW1), number of piglets born alive from second to last parity (NBA2+), litter birth weight from second to last parity (LTBW2+), number of piglets weaned from second to last parity (NPW2+) and litter weaning weight from second to last parity (LTWW2+), and to identify the percentages of animals (the top 10%, 25%, and 50%) for first parity and sums of second and later parity traits. Methods: The 9,830 records consisted of 2,124 Landrace (L), 724 Yorkshire (Y), 2,650 LY, and 4,332 YL that had their first farrowing between July 1989 and December 2013. The 8-trait animal model included the fixed effects of first farrowing year-season, additive genetic group, heterosis of the sow and the litter, age at first farrowing, and days to weaning (NPW1, LTWW1, NPW2+, and LTWW2+). Random effects were animal and residual. Results: Heritability estimates ranged from $0.08{\pm}0.02$ (NBA1 and NPW1) to $0.29{\pm}0.02$ (NPW2+). Genetic correlations between reproduction traits in the first parity and from second to last parity ranged from $0.17{\pm}0.08$ (LTBW1 and LTBW2+) to $0.67{\pm}0.06$ (LTWW1 and LTWW2+). Phenotypic correlations between reproduction traits in the first parity and from second to last parity were close to zero. Rank correlations between LTWW1 and LTWW2+ estimated breeding value tended to be higher than for other pairs of traits across all replacement percentages. Conclusion: These rank correlations indicated that selecting boars and sows using genetic predictions for first parity reproduction traits would help improve reproduction traits in the second and later parities as well as lifetime productivity in this swine population.