Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Variation of Glucosinolate Contents among Domestic Broccoli (Brassica oleracea L. var. italica) Accessions

국내 브로콜리(Brassica oleracea L. var. italica) 유전자원 내 Glucosinolate 함량 변이

  • Lee, Jun Gu (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kwak, Jung-Ho (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Um, Yeong Cheol (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Sang Gyu (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Jang, Yoon-Ah (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Choi, Chang Sun (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 이준구 (국립원예특작과학원 채소과) ;
  • 곽정호 (국립원예특작과학원 채소과) ;
  • 엄영철 (국립원예특작과학원 채소과) ;
  • 이상규 (국립원예특작과학원 채소과) ;
  • 장윤아 (국립원예특작과학원 채소과) ;
  • 최장선 (국립원예특작과학원 채소과)
  • Received : 2012.07.02
  • Accepted : 2012.08.03
  • Published : 2012.12.31
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Abstract

A total of 95 broccoli (Brassica oleracea L. var. italica) accessions were evaluated for the identification of desulfo-glucosinolates and their content variation in the flower head using ultra performance liquid chromatography, to select the potentially functional broccoli breeding lines. The six individual desulfo-glucosinolates, including progoitrin, glucoraphanin, sinigrin, gluconapin, glucobrassicanapin, and glucobrassicin, were commonly identified, based on the chromatogram peak comparison with those of the nine individual glucosinolate standards. The total glucosinolate contents varied from 4.2 to $29.0{\mu}mol{\cdot}g^{-1}$ DW and the glucoraphanin (1.6 to $13.9{\mu}mol{\cdot}g^{-1}$ DW) was confirmed as a major constituent in the total glucosinolate profile among the six identified individual glucosinolate species, whereas the progoitrin, which was only detected in 13 accessions, showed accession-specific variation and negative correlation with glucoraphanin content. It was also revealed that the four major glucosinolates, such as glucobrassicanapin, glucoraphanin, glucobrassicin, and gluconapin, affected major content variation and showed higher positive inter-correlation. These results might be used for the selection of potential breeding materials as functional broccoli germplasm through the further evaluation on the stability and reproducibility of glucosinolate profile depending on environmental factors or cultural managements using the selected accessions.

본 연구는 UPLC를 이용하여 국내 브로콜리 육성계통 95점의 화구 내 desulfo-glucosinolate를 표준물질과 비교하여 정량 분석하고 그 함량변이를 평가하여, 궁극적으로 고기능성 국내 브로콜리 품종 육성의 기초자료로 이용하고자 수행되었다. 브로콜리 화구에서 9종의 표준물질과 비교하여 progoitrin, glucoraphanin, sinigrin, gluconapin, glucobrassicanapin 및 glucobrassicin 등 6종의 glucosinolate가 동정되었다. 전체 브로콜리 육성계통 95점에 대해서 총 glucosinolate 함량은 $4.2-29.0{\mu}mol{\cdot}g^{-1}$ DW의 변이를 보였다. 총 glucosinolate 중 glucoraphanin의 함량은 $1.6-13.9{\mu}mol{\cdot}g^{-1}$ DW로 가장 높은 비율을 차지하였고 progoitrin은 제한적인 13개의 계통에서만 특이적으로 함유되어 있었으며 glucoraphanin의 함량과는 부의 상관관계를 보였다. 동정된 glucosinolate 중 glucobrassicanapin, glucoraphanin, glucobrassicin 및 gluconapin의 4종 물질이 전체 브로콜리 계통의 함량변이에 크게 영향하였고 이들 4종 물질 상호 간에는 높은 정의 상관관계가 있음이 확인되었다. 본 연구결과에서 선발된 브로콜리 유망계통에 대해서는, 향후 재배 작형 및 연차간 물질함량의 안정성 평가와 유전자원 간 추가적인 비교평가 연구를 통하여 고기능성 육종소재로의 개발이 가능할 것으로 판단된다.

Keywords

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