• Journal of Microbiology and Biotechnology
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• 제29권12호
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• pp.1947-1956
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• 2019

The gut microbiome influences the health and well-being of dogs. However, little is known about the impact of breed on the fecal microbiome composition in dogs. Therefore, we aimed to investigate the differences in the fecal microbiome in three breeds of dog fed and housed under the same conditions, namely eight Maltese (8.0 ± 0.1 years), eight Miniature Schnauzer (8.0 ± 0.0 years), and nine Poodle dogs (8.0 ± 0.0 years). Fresh fecal samples were collected from the dogs and used to extract metagenomic DNA. The composition of the fecal microbiome was evaluated by 16S rRNA gene amplicon sequencing on the MiSeq platform. A total of 840,501 sequences were obtained from the 25 fecal samples and classified as Firmicutes (32.3-97.3% of the total sequences), Bacteroidetes (0.1-62.6%), Actinobacteria (0.2-14.7%), Fusobacteria (0.0-5.7%), and Proteobacteria (0.0-5.1%). The relative abundance of Firmicutes was significantly lower in the Maltese dog breed than that in the other two breeds, while that of Fusobacteria was significantly higher in the Maltese than in the Miniature Schnauzer breed. At the genus level, the relative abundance of Streptococcus, Fusobacterium, Turicibacter, Succinivibrio, and Anaerobiospirillum differed significantly among the three dog breeds. These genera had no correlation with age, diet, sex, body weight, vaccination history, or parasite protection history. Within a breed, some of these genera had a correlation with at least one blood chemistry value. This study indicates that the composition of the fecal microbiome in dogs is affected by breed.

• Asian-Australasian Journal of Animal Sciences
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• 제21권8호
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• pp.1171-1177
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• 2008

The performance of growing rabbits fed Ruzi grass (Brachiaria ruziziensis), head lettuce (Lactuca sativa) residue, Mimosa pigra and water spinach (Ipomoea aquatica) was studied in an experiment using 64 rabbits (4 males and 4 females per treatment) of 2 breeds, New Zealand White and a crossbred between New Zealand White and native breed. The rabbits had an average initial weight of 668 g, were about 6 weeks old and were housed in individual pens. The foliages were fed ad libitum and a commercial concentrate was fed at a restricted level of 2% of body weight on a dry matter (DM) basis. In the digestibility experiment, the rabbits, 4 per foliage and males only, were fed the same foliages as in the growth experiment but without concentrate. Daily weight gain was lower in the group fed Ruzi grass, 14.8 g/d (p<0.001) compared to 17.6, 18.5 and 18.4 g/d for head lettuce, Mimosa pigra and water spinach, respectively. Feed intake and feed conversion ratio were lowest for the rabbits fed water spinach, 66 g DM/d and 3.6 kg DM/kg live weight, respectively. The New Zealand White breed had a higher daily gain than the crossbred rabbits (p<0.05), 18.0 and 16.7 g/d, respectively. There were no significant differences in feed intake, growth or feed conversion ratio due to sex. The digestibility coefficients of DM, organic matter, crude protein, crude fiber, neutral detergent fiber and acid detergent fiber were significantly lower (p<0.001) in the rabbits fed Ruzi grass. Breed and sex had no effect on digestibility. In conclusion, feeding head lettuce residue, Mimosa pigra and water spinach resulted in higher growth rate and digestibility than feeding Ruzi grass and can be recommended as alternative feeds.

• Asian-Australasian Journal of Animal Sciences
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• 제26권7호
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• pp.905-910
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• 2013

This study was performed to determine the influence of pig breed and gender on the ultimate pH and physicochemical properties of pork. The correlations between pH and pork quality traits directly related to carcass grade, and consumer's preference were also evaluated. The pH and meat grading scores for cold carcasses of 215 purebred pigs (Duroc, Landrace, and Yorkshire) from four different farms were obtained. Meat quality parameters of the pork loin were analyzed. Duroc and female animals were more affected compared to other breeds and male pigs. Duroc animals had the highest ultimate pH, carcass back fat thickness, marbling scores, yellowness, and fat content (p<0.05). Landrace pigs had the highest color lightness and cooking loss values (p<0.05). Among all trait parameters, marbling scores showed the highest significant differences when evaluating the impact of breed and gender on meat quality characteristics (p<0.001). Ultimate pH was positively correlated with carcass weight (0.20), back fat thickness (0.19), marbling score (0.17), and color score (0.16) while negatively correlated with cooking loss (-0.24) and shear force (-0.20). Therefore, pork samples with lower ultimate pH had lower cooking loss, higher lightness, and higher shear force values irrespective of breed.

• 한국임상수의학회지
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• 제33권6호
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• pp.340-345
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• 2016

The present study determined the normal reference ranges for the femoral and tibial joint orientation angles of small-breed dogs. For this purpose, 60 each of cadaveric canine femurs and tibias from normal small-breed dogs (Maltese, Poodle, Shih Tzu, Yorkshire Terrier) were examined with radiographs and photographs. Axial and frontal radiographs and photographs of each bone were obtained, from which anteversion and inclination angles, anatomic lateral proximal and distal femoral angles (aLPFA and aLDFA), mechanical lateral proximal and distal femoral angles (mLPFA and mLDFA), and mechanical medial proximal and distal tibial angles (mMPTA and mMDTA) were measured. The 95% CI for radiographic values of all femurs and tibiae were anteversion angle, $23.4-27.4^{\circ}$; inclination angle, $128.4-130.4^{\circ}$; aLPFA, $117.8-122.1^{\circ}$; aLDFA, $93.7-95.2^{\circ}$; mLPFA $113.8-117.3^{\circ}$; mLDFA $99.2-100.5^{\circ}$; mMPTA $96.8-98.5^{\circ}$; mMDTA $89.4-90.7^{\circ}$. The Maltese had a larger anteversion angle than the Poodle and the Yorkshire Terrier and a larger mLPFA than the Poodle. In the comparison between the radiographs and the photographs, significant differences were found in the anteversion angle, mLPFA, mMPTA, and mMDTA. The established normal reference values might be useful for determining whether a valgus or varus deformity of the femur or the tibia is present and if so, the degree of angular correction needed.

• 한국임상수의학회지
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• 제39권3호
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• pp.100-106
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• 2022

The center of rotation of angulation-based leveling osteotomy (CBLO) has been introduced for the stabilization of cranial cruciate ligament rupture (CCLR) in small-breed dogs. This technique can be applied to the tibia without damaging its tuberosity. Although different designs of CBLO plates are available, tibial plateau leveling osteotomy (TPLO) plates have been still used for stabilization during CBLO. To the best of our knowledge, no studies have reported the effects of TPLO plates on the postoperative limb alignment after CBLO. Therefore, the present study (January 2020 to February 2021) aimed to compare the postoperative outcomes (postoperative tibial plateau angle [TPA] and tibial alignment) in patients receiving CBLO and TPLO plates during CBLO. Paired stifle joints (n = 16) were obtained from eight toy-breed cadaver dogs (mean weight, 4.4 kg) that underwent CBLO. The joints were randomly assigned to receive the CBLO (CBLO group) or TPLO plates (TPLO group). Pre-and postoperative radiographs were obtained, and the dissected tibiae were evaluated. The following postoperative parameters were compared to evaluate the surgical outcome: TPA, osteotomy location, mechanical medial proximal tibia angle, inter-segment gap, and tibial plateau translation. No significant differences were found in the postoperative alignment between the two groups. Therefore, TPLO plates may be considered as a viable alternative in toy-breed dogs undergoing CBLO.

• 한국환경과학회지
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• 제31권12호
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• pp.1139-1142
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• 2022

The present study aimed to examine the effect of dietary Ptecticu tenebrifer powder mixtures as pet dogfeed ingredients on crude fat and ash digestibility. Three groups of feeds Feed A, Feed B, and Feed C supplied from three farms were fed to a total of 45 dogs. The dietary Ptecticu tenebrifer powder mixture were prepared by mixing 25 g of Ptecticu tenebrifer powder with 100 g of canned food. Feed A, Feed B, and Feed C containing dietary Ptecticu tenebrifer powder mixtures were fed to 15 dogs of each breed of bichon, poodle, and chihuahua that were divided into three groups following a completely randomized design. For measuring the crude fat and crude ash digestibility, manure of each dog breed from each group were collected. Crude fat digestibility was not statistically significant among the dog breeds fed with feed C (p>0.05), but overall there was a statistical difference between the feed and the group by dog breed (p<0.05). In terms of crude ash digestibility, the three types of feed showed differences with respect to dog breeds (p<0.05). However, the group with no significant difference was observed in Feed B by dog breed (p>0.05). In conclusion, feeding Ptecticu tenebrifer powder mixture to dog breeds had no positive effect on the crude fat and ash digestibility and can be used as pet dogfeed ingredients.

• 한국임상수의학회지
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• 제40권1호
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• pp.31-37
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• 2023

Vertebral left atrial size (VLAS) is an important indicator to predict myxomatous mitral valve degeneration (MMVD) in dogs. When the caudal margin of cardiac silhouette and the dorsal margin of caudal vena cava (CdVC) could not be seen exactly, another way to evaluate VLAS is needed. The objective of this study was to assess whether a new modified VLAS (m-VLAS) could be used as an indicator to predict MMVD in 57 small breed dogs with MMVD. The m-VLAS was also used to classify American College of Veterinary Internal Medicine staging groups and left heart enlargement confirmed with echocardiograph (EchoLHE) groups. The m-VLAS was measured as the distance from the ventral aspect of the carina to the dorsal aspect of the intersection of the cardiac silhouette and the farthest LA caudal margin, not the CdVC, followed by drawing the same line beginning at the cranial edge of T4. Based on VLAS values and m-VLAS values measured for dogs with MMVD, correlations between these values and left heart enlargement groups were then evaluated. There were significant differences in both the VLAS and the m-VLAS between EchoLHE groups. The AUC of the ROC curve of the m-VLAS to detect EchoLHE was higher than that of the VLAS. The optimal cutoff value for the m-VLAS was >2.7, which had a higher specificity (86.84%) than the VLAS specificity (71.05%). This study reveals that a new m-VLAS is a more specific indicator than the VLAS for predicting left side heart enlargement in small breed dogs. Therefore, the m-VLAS can be used as a clinically useful radiographic measurement alternative to or better than the VLAS.

• Asian-Australasian Journal of Animal Sciences
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• 제16권5호
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• pp.655-659
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• 2003

Forty eight lambs (24 each of Malpura and Mutton synthetic breeds) were weaned at 90 days of age and divided into two groups. One group of 12 lambs from each of the two breeds was maintained on low energy (52% TDN) and the other group of 12 on high energy (58% TDN) feedlot ration until 6 of the lambs attained 20 kg and the other 6 attained 25 kg live weight in each of the two groups. Daily feed intake and weekly body weights were recorded. Conventional metabolism trials were conducted on the two breeds and the two rations. The growth rate was found to be higher for lambs on high energy ration (p<0.01). The growth rate was also higher for higher target weight groups (p<0.01). The breed did not significantly affect the live weight gains. Mutton synthetic lambs required less number of days to reach 20 kg live weights but took more time to reach 25 kg target weight as compared to Malpura lambs (p<0.01). The lambs of both the breeds reached 25 kg live weight earlier on high energy than those on low energy ration (p<0.01). The dry matter intake, irrespective of breed, was significantly higher (4.57% or 93.4 g per kgW$^{0.75}$) on low energy than that on high energy ration (4.20% or 87.2 g per kg W$^{0.75}$). The digestibility coefficients of all the nutrients excepting crude fibre were significantly higher on high energy diet irrespective of the breed. Lambs on low energy ration, however, digested the crude fibre more than those on the high energy ration (p<0.05). Although there were no significant differences in the intakes of nitrogen, calcium and phosphorus, the balance of nitrogen was higher on high energy ration. The DCP and TDN values were 9.70 and 52.76% for low energy and 9.89 and 57.68 % for high energy ration, respectively. The dressing percentages on live weight basis were 50.2 on low and 51.6 on high energy ration, 50.5 at 20kg and 51.3 at 25 kg slaughter weight and 51.0 in Malpura and 50.8 in Mutton synthetic lambs, respectively. The percent of bones in the carcass was higher on low energy ration in Mutton synthetic lambs at 20 kg slaughter weight than others. It was concluded that the performance of the lambs in respect of mutton production was significantly better on high energy ration fed upto 25 kg slaughter weight with no or little breed differences.

• Asian-Australasian Journal of Animal Sciences
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• 제20권12호
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• pp.1798-1804
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• 2007

The objective of this study was to assess the association of polymorphisms in MSTN and MYF5 genes with growth traits in three Chinese cattle breeds. Only one homozygous animal with BB genotype at MSTN locus was observed in Jiaxian population which was at Hardy-Weinberg disequilibrium (p<0.05). The frequencies of allele A at MSTN locus and allele B at MYF5 locus in the three Chinese breeds were 0.9550/0.9730/0.9720 and 0.8275/0.7581/0.7523, respectively. Allele A at MSTN locus and allele B at MYF5 locus were dominant in these three populations. No statistically significant differences in growth traits were observed between the genotypes of the Jiaxian breed at MSTN and MYF5 loci and the Nanyang breed at MYF5 locus. However, there were statistically significant differences between the genotypes at MSTN locus of the Nanyang breed for WH, HG, HGI and HGBLR (p<0.05), and of the Qinchuan breed for BLI (p<0.05). The SNP in MYF5 had significant effects on WH and HHC of Qinchuan animals (p<0.05). These results suggest that MSTN and MYF5 are strong candidate genes that influence growth traits in cattle. Other SNPs of MSTN and MYF5 or other linked genes should also be studied, which could lead to the development of selection plans to improve the performance of Chinese cattle and also promote the breeding of genuine beef cattle in China.

• Journal of Animal Science and Technology
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• 제45권5호
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• pp.841-846
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• 2003

Halothane 검정에 의한 L(Landrace) 및 Lw (Largewhite)의 halothane 양성돈 출현율은 각각 5.8, 6.6 %로 두 품종간에 큰 차이가 없었으며 음성과 양성돈의 중간반응을 나타내는 의양성돈은 20.5 %의 출현율을 보인 L과 Lw의 13.2 %에 비해 높았으나 음성돈 출현율은 L 및 Lw가 각각 76.1, 80.1 %로 Lw가 약간 높았다. hal-gene 분석결과는 L품종만이 hetro형이 출현하였을 뿐 전 조사 품종에서 halothane 양성돈은 나타나지 않았다. 품종별 pH1은 KNP (Korean Native Pig)가 L, Lw 및 H(Hybrid)에 비해 약간 높았으나 통계적 유의성은 없었다. pHu는 KNP와 H가 Lw 및 L에 비해 유의 (p〈0.05)하게 높았으나 KNP와 H품종사이에는 유의적인 차이가 없었다. NPPC 기준 육색은 L이 Lw 및 KNP에 비해 유의적(p〈0.05)으로 낮았고 H와는 유의차가 없었으며 CIE L*값 또한 같은 경향을 보여 L품종이 다른 품종보다 색깔이 창백한 고기를 생산할 기능성이 큰 것으로 사료되었다. 보수성은 재래종인 KNP가 H에 비해 유의적으로(p〈0.05)으로 높았으며 개량종인 Lw, L 및 H에서는 유의적인 차이가 없었다.