• Asian Pacific Journal of Cancer Prevention
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• 제15권10호
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• pp.4233-4238
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• 2014

Background: The aim of this study was to investigate the relationship of body mass index with overall and progression-free survival as well as other prognostic factors of breast cancer in patients with non-metastatic breast cancer. Materials and Methods: We retrospectively reviewed 456 patients diagnosed with breast cancer in the Radiation Oncology department of Kayseri Teaching Hospital between 2005 and 2013. We investigated relationship of body mass index with prognosis and other prognostic factors. Results: The study included 456 patients (447 women and 9 men). Mean age at presentation was 55.6 years. Of the cases, 96.9% underwent modified radical mastectomy and 95.0% received chemotherapy, while 82.4% received radiotherapy and 60.0% were given hormone therapy. Body mass index was >25 mg/kg2 in 343 cases. Five- and 10-years overall survival rates were 77% and 58% whereas progression-free survival rates were 65% and 49%, respectively. In univariate analyses, factors including stage (p=0.046), tumor diameter (p=0.001), lymph node metastasis (p=0.006) and body mass index (p=0.030) were found to be significantly associated with overall survival, while perinodal involvement was found to be significantly associated with progression-free survival (p=0.018). In multivariate analysis, stage (p=0.032; OR: 3.8; 95% CI: 1.1-13), tumor diameter (p<0.000; OR: 0.0; 95% CI: 0.0-0.3), lymph node metastasis (p=0.005; OR: 0.0; 95% CI: 0.0-0.5) and BMI (p=0.027; OR: 0.02; 95% CI: 0.0-0.8) remained as significantly associated with OS. Conclusions: In our study, it was seen that overall survival time was shorter in underweight and obese patients when compared to normal weight patients.

• Asian Pacific Journal of Cancer Prevention
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• 제15권10호
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• pp.4311-4317
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• 2014

Background: Nano-biotechnology is recognized as offering revolutionary changes in the field of cancer therapy and biologically synthesized gold nanoparticles are known to have a wide range of medical applications. Materials and Methods: Gold nanoparticles (GNPs) were biosynthesized with an aqueous extract of the red alga Corallina officinalis, used as a reducing and stabilizing agent. GNPs were characterized using UV-Vis spectroscopy, transmission electron microscopy (TEM), energy dispersive analysis (EDX) and Fourier transform infra-red (FT-IR) spectroscopy and tested for cytotoxic activity against human breast cancer (MCF-7) cells cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum, considering their cytotoxicty and effects on cellular DNA. Results: The biosynthesized GNPs were $14.6{\pm}1nm$ in diameter. FT-IR analysis showed that the hydroxyl functional group from polyphenols and carbonyl group from proteins could assist in formation and stabilization. The GNPs showed potent cytotoxic activity against MCF-7 cells, causing necrosis at high concentrations while lower concentrations were without effect as indicated by DNA fragmentation assay. Conclusions: The antitumor activity of the biosynthesized GNPs from the red alga Corallina officinalis against human breast cancer cells may be due to the cytotoxic effects of the gold nanoparticles and the polyphenolcontent of the algal extract.

• 한국환경복원기술학회지
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• 제11권3호
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• pp.116-128
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• 2008

Vegetation structure of Jangtaesan Natural Recreation Forest in Daejeon City was investigated to suggest a preferable management plan. Vegetation survey was carried out with thirty plots. Adopting phytosociological method, plant communities were classified into twinfolds of natural and afforested area. A natural forest were composed of Quercus mongolica, Quercus variabilis, and Pinus densiflora community. Afforested area were with Metasequoia glyptostroboides, Larix leptolepis, Pinus koraiensis, and Castanea crenata community. The importance value of Quercus mongolica, Quercus variabilis, Pinus densiflora, and Quercus serrata in natural forest were 59.69%, 48.32%, 39.10%, 21.53%, respectively. According to diameter of breast hieght analysis results, it is expected that the occupancy of Quercus mongolica, Pinus densiflora, and Quercus variabilis would be continuously increased. Based on the findings of the study, management plan for natural forest and afforested area was recommended in line with the progress of natural ecology.

• 대한영상의학회지
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• 제83권3호
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• pp.620-631
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• 2022

목적 유방암 환자의 수술 전 자기공명영상에서 림프절 전이를 시사하는 소견들에 따른 진단 성적을 알아보고자 한다. 대상과 방법 수술 전 유방 자기공명영상을 촬영하고 유방암 수술을 시행한 192명의 환자를 후향적으로 분석하였다. 영상 소견에서 림프절의 크기와 장경/단경의 비율, 피질의 두께와 모양, 변연, 수질의 소실, 비대칭성, T2 강조영상에서의 신호강도, 이른 조영증강의 정도, 조영증강의 역학을 조사하였다. 수신자판단특성곡선 분석, 카이 분석과 t-검정, 맥니마 검정을 이용하여 통계분석을 시행하였다. 결과 단경의 증가, 피질의 불규칙한 모양과 피질 두께의 증가, 수질의 소실, 비대칭성, 피질의 불규칙한 변연 그리고 T2 강조영상에서의 낮은 신호강도는 전이를 시사하는 의미 있는 소견이었다. 이중 단경과 피질의 두께에 대해 수신자판단특성곡선 분석으로 각각 8.05 mm와 2.75 mm로 절단값을 얻었다. 2.75 mm 이상의 피질 두께, 피질의 불규칙한 모양은 맥니마 검정으로 다른 소견들과 비교할 때 민감도의 유의한 차이를 보였다. 피질의 불규칙한 변연(100%)은 가장 높은 특이도를 보였다. 결론 유방 자기공명영상의 전이 림프절 분석에서 2.75 mm 이상의 피질 두께와 피질의 불규칙한 모양은 다른 소견들보다 높은 민감도를 보이고 피질의 불규칙한 변연은 가장 높은 특이도를 보이는 소견이다.

• 한국산림과학회지
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• 제108권4호
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• pp.600-609
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• 2019

본 연구는 우리나라 소나무와 일본잎갈나무의 지역별 형상비를 고려한 임분 밀도 관리의 필요성과 관리 방안을 제시하기 위하여 수행되었다. 전국을 대상으로 소나무 2,112본, 일본잎갈나무 2,030본을 벌채하여 흉고직경 및 수고, 지하고를 측정하고 지역별 형상비(수고/흉고직경)를 비교 분석하였다. 소나무의 형상비는 강원지방 소나무 72.3%, 중부지방소나무 64.0%, 소나무 평균 70.8%로 강원지방소나무가 중부지방소나무에 비하여 높게 나타났다. 지역별로는 영주 78.4%, 영월 77.5%순으로 높게 나타났으며, 직경별로는 소경목 90.4%, 중경목 71.7%, 대경목 56.1%로 나타났다. 형상비는 밀도가 높아질수록 수간의 직경에 대한 수고의 비율(형상비)이 높아지며, 70% 이하면 안정성이 높으나 80%를 초과하면 풍해 및 폭설피해로 위험해지기 때문에 일부 지역에서는 임분 밀도관리가 필요하다. 일본잎갈나무의 전체 평균 형상비는 90.6%이며, 지역별로는 평창 108.5%, 영주 105.4%, 단양 100.5% 순으로 나타났다. 경급별로는 소경목 104.9%, 중경목 92.7%, 대경목 73.4%로 전체적으로 일본잎갈나무의 형상비가 80% 이상으로 풍해 및 폭설피해의 위험에 노출되어 있어 빠른 시일 내에 적정 임분 밀도의 관리의 시업이 요구되어 진다.

• 농업과학연구
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• 제50권2호
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• pp.207-222
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• 2023

This study investigated 62 nested quadrat plots of Jinaksan to identify community classification and changes of the vegetation by using the phytosocial method and analyzed importance values. Vegetation types were classified into 8 communities: Quercus mongolica community, Q. variableis community, Q. aliena community, Pinus densiflora, Q. acutissima, Zelkova serrata, Carpinis laxiflora, and C. tschonoskii. The significance value was highest in Q. mongolica (62.75) followed by P. densiflora (55.16), Q. variabilis community (25.03), Z. serrata (22.17), Q. aliena (18.30), Prunus serrulata var. pubescens (16.86), C. laxiflora (13.25), Q. acutissima (10.72), C. tschonoskii (10.08), Q. serrata (8.02), Fraxinus sieboldiana (6.93), Acer pseudosieboldianum (6.73), and Styrax obassis (5.73). Quercus mongolica displayed a stable distribution pattern, presenting a reverse J-shaped curve from the diameter at breast height (DBH) analysis, and it was judged that current state would be maintained for a certain period. In addition, P. densiflora is expected to dominate for the time being and Quercus species are expected to gradually decrease.

• Korean Journal of Radiology
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• 제22권7호
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• pp.1021-1033
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• 2021

Objective: To assess the expression of vascular normalization genes in different molecular subtypes of breast cancer and to determine whether molecular subtypes with a higher vascular normalization gene expression can be identified using dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) and intravoxel incoherent motion (IVIM) diffusion-weighted imaging (DWI). Materials and Methods: This prospective study evaluated 306 female (mean age ± standard deviation, 50 ± 10 years), recruited between January 2014 and August 2017, who had de novo breast cancer larger than 1 cm in diameter (308 tumors). DCE MRI followed by IVIM DWI studies using 11 different b-values (0 to 1200 s/mm²) were performed on a 1.5T MRI system. The Tofts model and segmented biexponential IVIM analysis were used. For each tumor, the molecular subtype (according to six [I-VI] subtypes and PAM50 subtypes), expression profile of genes for vascular normalization, pericytes, and normal vascular signatures were determined using freshly frozen tissue. Statistical associations between imaging parameters and molecular subtypes were examined using logistic regression or linear regression with a significance level of p = 0.05. Results: Breast cancer subtypes III and VI and PAM50 subtypes luminal A and normal-like exhibited a higher expression of genes for vascular normalization, pericyte markers, and normal vessel function signature (p < 0.001 for all) compared to other subtypes. Subtypes III and VI and PAM50 subtypes luminal A and normal-like, versus the remaining subtypes, showed significant associations with K^trans, k_ep, v_p, and IAUGC_BN90 on DEC MRI, with relatively smaller values in the former. The subtype grouping was significantly associated with D, with relatively less restricted diffusion in subtypes III and VI and PAM50 subtypes luminal A and normal-like. Conclusion: DCE MRI and IVIM parameters may identify molecular subtypes of breast cancers with a different vascular normalization gene expression.

• Asian Pacific Journal of Cancer Prevention
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• 제16권14호
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• pp.6039-6046
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• 2015

Aims: To investigate effect of metallic nanoparticles, silver (AgNPs) and gold nanoparticles (AuNPs) as antitumor treatment in vitro against human breast cancer cells (MCF-7) and their associated mechanisms. This could provide new class of engineered nanoparticles with desired physicochemical properties and may present newer approaches for therapeutic modalities to breast cancer in women. Materials and Methods: A human breast cancer cell line (MCF-7) was used as a model of cells. Metallic nanoparticles were characterized using UV-visible spectra and transmission electron microscopy (TEM). Cytotoxic effects of metallic nanoparticles on MCF-7 cells were followed by colorimetric SRB cell viability assays, microscopy, and cellular uptake. Nature of cell death was further investigated by DNA analysis and flow cytometry. Results: Treatment of MCF-7 with different concentrations of 5-10nm diameter of AgNPs inhibited cell viability in a dose-dependent manner, with IC50 value of $6.28{\mu}M$, whereas treatment of MCF-7 with different concentrations of 13-15nm diameter of AuNPs inhibited cell viability in a dose-dependent manner, with IC50 value of $14.48{\mu}M$. Treatment of cells with a IC50 concentration of AgNPs generated progressive accumulation of cells in the S phase of the cell cycle and prevented entry into the M phase. The treatment of cells with IC50 concentrations of AuNPs similarly generated progressive accumulation of cells in sub-G1 and S phase, and inhibited the entrance of cells into the M phase of the cell cycle. DNA fragmentation, as demonstrated by electrophoresis, indicated induction of apoptosis. Conclusions: Our engineered silver nanoparticles effectively inhibit the proliferation of human breast carcinoma cell line MCF-7 in vitro at high concentration ($1000{\mu}M$) through apoptotic mechanisms, and may be a beneficial agent against human carcinoma but further detailed study is still needed.

• 한국산림과학회지
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• 제26권1호
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• pp.13-18
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• 1975

본시험(本試驗)은 죽간재적(竹稈材積)에 영향(影響)하는 형질(形質)을 정확(正確)하게 파악(把握)하기 위(爲)하여 계획(計劃)되었다. 그리고 왕대의 수개형질상호간(數個形質相互間)의 표현형상관(表現型相關), 유전상관(遺傳相關) 및 환경상관(環境相關)을 구명(究明)하고져 공분산분석(共分散分析) 되었으며 Dewey and Lu의 편회귀분석법(偏回歸分析法)으로 각형질(各形質)이 죽간재적(竹稈材積)에 미치는 효과(効果) 즉(即) 경로계수(經路係數)를 산출(算出)하였다. 조사형질(調査形質)은 흉고직경(胸高直徑), 죽간고(竹稈高), 지상고(枝上高), 지하경(地下莖), 절수(節數), 근원직경(根元直徑) 및 죽간재적(竹稈材積)이며 각(各) 형질(形質)의 평균치(平均値), 표준오차(標準誤差), 분산(分散) 및 표준편차(標準偏差)는 제(第)1표(表)와 같고 7개형간(個形間)의 표현형(表現型), 유전(遺傳) 및 환경상관(環境相關)을 분산분석(分散分析)한 결과(結果)는 제(第)2표(表)와 같이 계통간(系統間)에는 고도(高度)의 유의성(有意性)이 인정(認定)되었으나 몇몇 반복간(反覆間)에는 유의성(有意性)이 인정(認定)되지 않았다. 각(各) 형질(形質)의 유전력(遺傳力)을 추정(推定)한바 흉고직경(胸高直徑)과 근원직경(根元直徑)은 유전력(遺傳力)이 높고 기타형질(其他形質)의 유전력(遺傳力)은 제(第)4표(表)에서와 같이 보다 낮으며 환경상관(環境相關)은 표현형상관(表現型相關), 유전상관(遺傳相關)보다 전체적(全體的)으로 낮다. 그리고 죽간재적(竹稈材積)에 대(對)하여 근원직경(根元直徑)과 흉고직경(胸高直徑)은 높은 상관관계(相關關係)를 나타내었다. 형질별(形質別) 상관관계(相關關係), 경로계수(經路係數) 및 죽간재적(竹稈材積)에 미치는 직접(直接), 간접효과(間接効果)는 도표(圖表) 1과 제(第)5표(表)에서 볼 수 있으며 결과적(結果的)으로 본시험(本試驗)에서는 죽간재적수량(竹稈材積收量)은 근원직경(根元直徑)과 흉고직경(胸高直徑)에 의(依)하여 좌우(左右)될 수 있다는 것을 알았다.

• Radiation Oncology Journal
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• 제35권2호
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• pp.121-128
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• 2017

Purpose: To report the results of a correlation analysis of skin dose assessed by in vivo dosimetry and the incidence of acute toxicity. This is a phase 2 trial evaluating the feasibility of intraoperative radiotherapy (IORT) as a boost for breast cancer patients. Materials and Methods: Eligible patients were treated with IORT of 20 Gy followed by whole breast irradiation (WBI) of 46 Gy. A total of 55 patients with a minimum follow-up of 1 month after WBI were evaluated. Optically stimulated luminescence dosimeter (OSLD) detected radiation dose delivered to the skin during IORT. Acute toxicity was recorded according to the Common Terminology Criteria for Adverse Events v4.0. Clinical parameters were correlated with seroma formation and maximum skin dose. Results: Median follow-up after IORT was 25.9 weeks (range, 12.7 to 50.3 weeks). Prior to WBI, only one patient developed acute toxicity. Following WBI, 30 patients experienced grade 1 skin toxicity and three patients had grade 2 skin toxicity. Skin dose during IORT exceeded 5 Gy in two patients: with grade 2 complications around the surgical scar in one patient who received 8.42 Gy. Breast volume on preoperative images (p = 0.001), ratio of applicator diameter and breast volume (p = 0.002), and distance between skin and tumor (p = 0.003) showed significant correlations with maximum skin dose. Conclusions: IORT as a boost was well-tolerated among Korean women without severe acute complication. In vivo dosimetry with OSLD can help ensure safe delivery of IORT as a boost.