• Natural Product Sciences
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• 제6권4호
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• pp.199-200
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• 2000

A flavonol diglucoside was isolated from the leaves of Brassica juncea L. The structure of the compound was elucidated as isorhamnetin $3,7-di-O-{\beta}-D-glucopyranoside$ (1) on the basis of chemical and spectral evidence.

• 한국식생활문화학회지
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• 제27권6호
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• pp.719-729
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• 2012

We sought to study the qualities and scientific benefits of Dongchimi, a traditional Korean food. We compared and analyzed ingredients used for the appearance and storability of dongchimi - honggot (Brassica juncea czerniak et coss), cockscomb (Celosia cristata L.), and beet Beta vulgaris L.). We specifically examined the antioxidative and antibacterial activity of pigments from extracts of these ingredients. Distilled water ($H_2O$) and 1% citric acid were used to safely extract pigments. The antioxidative activity of the pigments was then measured for total phenolic compounds, SOD (Super Oxide Dismutase), and EDA (Electron Donation Ability) by DPPH. The antibacterial activity of was also assessed by a Paper disc solution. Our results show that the pigments had sufficient antioxidative activity and had antibacterial properties against Gram positive and negative bacteria. In particular, Cockscomb (used for enhancing color) contained the highest amount of polyphenol compounds and had the most efficient antioxidative activity.

• 한국식품위생안전성학회지
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• 제36권6호
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• pp.528-536
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• 2021

본 연구는 십자화과 채소인 갓(Brassica juncea) 추출물을 이용하여 지표성분인 sinigrin의 함량을 분석하고, 내분비계 교란물질 환경호르몬인 비스페놀 A (BPA)로 분화를 유도한 3T3-L1 전구지방세포에서 갓 추출물과 sinigrin 처리에 대한 지방세포 분화 및 활성산소종(ROS) 생성 억제, 지방 생성 전사인자(PPARγ, C/EBPα, aP2)의 단백질 발현 감소 효능을 평가하였다. 연구 결과에 따르면 HPLC를 이용하여 측정한 갓 추출물 중 sinigrin의 함량은 21.27±0.2 mg/g 인 것으로 나타났다. BPA로 유도된 3T3-L1 전구지방세포에서 XTT assay 결과 sinigrin 180 µM 및 갓 추출물 300 ㎍/mL 농도에서 세포 독성을 보이지 않았으며, 지방세포 분화과정 중 세포 내 지방 축적량과 ROS 생성량을 비교하였을 때 갓과 sinigrin을 처리한 지방세포의 경우 지방축적량 및 ROS 생성량 모두 유의적으로 감소시키는 것으로 나타났다. 또한, 갓 추출물 및 sinigrin을 처리하였을 때 지방세포 분화를 조절하는 전사인자 PPARγ, C/EBPα 및 aP2의 발현이 억제됨을 확인하였다. 이 결과를 통해 갓은 환경호르몬 비스페놀 A로 유도된 지방세포 내 지방 축적 억제와 더불어 ROS 저감에 효과적으로 작용함을 확인하였다. 향후 sinigrin을 함유한 갓은 BPA로 인한 지질 대사 장애를 예방하는 천연물 유래 기능성 식품 소재로 활용할 가능성이 높은 것으로 기대되며, 추가로 임상 연구 및 작용기전 입증을 위한 in vivo 모델에서의 후속 연구가 진행되어야 할 것으로 사료된다.

• Applied Biological Chemistry
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• 제22권1호
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• pp.33-38
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• 1979

우리나라에서 채소(菜蔬)로 또 조미품(調味品)으로 많이 이용(利用)되고 있는 재래종(在來種)갓의 anthocyanin 색소(色素)의 구명(究明)을 위(爲)한 실험(實驗)에서 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 1. T.L.C.에 의(依)해 이종(二種)의 anthocyanin을 분리(分離)하였다. 2. 이들 anthocyanin의 구조구명(構造究明)을 위(爲)하여 Robinson 시험(試驗), Spectrophotometry, T.L. chromatography, 부분가수분해등(部分加水分解等)의 방법(方法)으로 각각 peonidin-3-glucoside와 peonidin-3-galctoside 임을 확인(確認)하였다.

• 생약학회지
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• 제32권2호통권125호
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• pp.140-144
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• 2001

The composition of essential oils in the leaves of three cultivars (Ban-Chung-Gat, Chung-Gat and Dolsan-Gat) of Brassica juncea L. were analyzed and their antifungal activity were investigated in this study. Allyl isothiocyanate, 2-phenyl ethyl isothiocyanate, 4-isothiocyanato-1-butene, 5-methyl isothiazole, benzene acetaldehyde, benzene propane nitrile and beta-ionone have been identified in all of the experimented oils. The main component of the oils from Ban-Chung-Gat and Chung-Gat was 2-phenyl ethyl isothiocyanate while allyl isothiocyanate was the representing compound in the oil of Dolsan-Gat. The antifungal activities of the oils were tested by micro broth dilution method and disc diffusion method. As the result the oils exhibited significant inhibiting activities against Aspergillus niger, A. flavus, Trichoderma viride, Candida albicans, C. utilis, C. tropicalis, Cryptococcus neoformans, Trichosporon mucoides, Trichophyton tonsurans and Geotrichum capitatum.

• Archives of Pharmacal Research
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• 제25권5호
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• pp.625-627
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• 2002

From the leaves of Brassica juncea, an radical scavenging isorhamnetin 7-Ο-glucoside on peroxynitrite and 1,1-diphenyl-2-picrylhydrazyl (DPPH) was isolated and characterized based on the spectroscopic evidence. The compound showed the peroxynitrite and DPPH scavenging activities with $IC_{50}$ values of 2.07$\pm$0.17 and 13.3 $\mu$M, respectively. Penicillamine and L-ascorbic acid as positive control exhibited radical scavenging activities with $IC_{50}$ values of 3.17$\pm$0.39 and 12.78 $\mu$M, respectively.

• 한국환경과학회지
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• 제31권7호
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• pp.569-577
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• 2022

This study aimed to evaluate the growth of Cichorium intybus, Brassica juncea, and Lactuca sativa at varying seed ratios, and to suggest an efficient management plan for sustainable indoor agriculture systems. It was treated with mixed sowing as follows: 30 chicory seeds (chicory alone: CC), 22 chicory seeds + 8 lettuce seeds (C₃L₁), 20 chicory seeds + 10 lettuce seeds (C₂L₁), 15 chicory seeds + 15 lettuce seeds (C₁L₁) as intraspecies, and 30 mustard seeds (mustard alone: MC), 22 mustard seeds + 8 lettuce seeds (M₃L₁), 20 mustard seeds + 10 lettuce seeds (M₂L₁), 15 mustard seeds + 15 lettuce seeds (M₁L₁) as interspecies. The study identified the competitive response in seed germination between Cichorium intybus and Lactuca sativa, and in the C₃L₁ experimental group, Lactuca sativa had the highest leaf length, root length, chlorophyll content, and fresh weight. Therefore, the higher the ratio of Cichorium intybus, the higher the growth and productivity of Lactuca sativa; however, higher the ratio of Lactuca sativa, the lower the growth of Cichorium intybus. Furthermore, the nitrogen and potassium content in the substrate was the highest in the C₃L₁ experimental grorp which had the highest seeding rate of the Cichorium intybus. Comparing the groups Brassica juncea and Lactuca sativa, the higher the seeding ratio of Lactuca sativa, the higher the growth and productivity of Brassica juncea. Therefore, a companion seeding of Brassica juncea and Lactuca sativa is beneficial; this could be effective in having a high seeding ratio of Lactuca sativa.

• Archives of Pharmacal Research
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• 제25권5호
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• pp.621-624
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• 2002

From the leaves of Brassica juncea, a new rare kaempferol 7-Ο-triglucoside isolated and characterized as kaempferol 7-Ο-$\beta$-D-glucopyranosyl-(1longrightarrow3)-[$\beta$-D-glucopyranosyl-(1longrightarrow6)]-glucopyranoside (1) based on the spectroscopic evidences. This compound was found to be a scavenger of 1, 1-diphenyl-2-picrylhydrazyl radical.

• Journal of Plant Biotechnology
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• 제46권2호
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• pp.114-118
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• 2019

Efficient protocol for plant shoot regeneration of Brassica juncea L. CZERN was established by using organic media components and growth stimulating factors of the vermicompost and coelomic fluids. Formulated organic plant tissue culture media (Vermicompost (30%) extracts supplemented with 20 mL/L coelomic fluid) have shown maximum shoot regeneration when compared with the Murashige and Skoog (MS) medium, which were supplemented with 1 mg/L 6-benzyladenine (BA) and 0.1 mg/L of Naphthaleneacetic acid (NAA). Cotyledon explants produced the highest shoot regeneration frequency from fourday-old germinated seedlings in comparison with non-germinated seedlings. The vermicompost extracts have proved to be the best organic plant growth media to induce shoots from cotyledons compared to the MS media. Statistically significant difference (P = 0.008) for the root length, shoot length (P=0.000350) and the leaves (P=0.375) of the mustard plantlets were analyzed successfully. The survival rate was 98% in the mustard cotyledons on the Vermicompost extract media and 63% on MS media respectively. The coelomic fluid also is much suitable to induce shoots from cotyledons at lower concentrations. It was also shown that the vermicompost extract, which comprised of humic acids along with coelomic fluid, affected shoot regeneration from the cotyledons. An efficient and organic shoot regeneration study was standardized and it can be applicable in the improvement of the economically important crops.

• 식물조직배양학회지
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• 제24권5호
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• pp.257-262
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• 1997

Brassica와 Raphanus 간에 속간 교잡종을 육성하기 위해서 34개의 교배 조합을 설정한 다음 수분 12일이 경과된 배주를 BA와 GA 1.0 ㎎/L가 첨가된 MS배지에 접종하고 배의 발아율을 조사하였다. BA나 GA 첨가는 배의 발아에 큰 영향을 미치지 않았다. 무 속의 '내병총태'와 '중국청피'를 부계로 교잡한 조합에서 배의 발아율이 낮았으나 갓(Brassica juncea)을 부계로 교잡한 조합에서는 비교적 배의 발아율이 높았다. 이러한 결과는 배주배양시 배지에 첨가한 casein hydrolysate, malt extract, BA, kinetin 및 glutamine이 배의 발아에 미치는 영향보다는 부계나 모계의 교잡조합에 따라 배의 발아율에 상당한 차이가 있다는 것을 의미한다.