• Applied Biological Chemistry
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• v.19 no.3
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• pp.130-138
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• 1976

In order to utilize the agricultural waste products, two strains of mold producing powerful cellulolytic enzyme were sereened from various soils, composts, rotten wood and others. The optimum condition of cellulase production was studied. The results obtained were summarized as follows. 1. Two strains of mold which showed remarkably high cellulolytic activity were identified as Aspergillus niger-SM 6 and Trichoderma viride-SM 10. 2. The highest cellulase production was obtained at pH 5.0-6.0 in 5 days. 3. Cellulase production in strain Aspergillus niger-SM 6 increased with the addition of C.M.C., $(NH_4)_2SO_4$, C.S.L., orange peel powder and rice hull. The rice hull, treated with 3N NaOH at $120^{\circ}C$ for 15 min. and neutralized with various acids, was used. Up to 50% of wheat bran could be substituted by the treated rice hull without any decrease of cellulase activity. 4. In the strain of Trichoderma viride-SM 10, cellulase production increased with the addition of C.M.C., $NH_4NO_3$, Vitamin-free casamino acid and orange peel powder, while the other carbon, nitrogen, phosphate sources, natural nutrients and organic substances gave no remarkable effect.

• Microbiology and Biotechnology Letters
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• v.38 no.4
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• pp.373-378
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• 2010

Studies on standardization and quality upgrade of nuruk which is a basic component in brewing are required to increase the quality level of Korean traditional rice wines and to develop the technology for practical use of it. It is important to isolate best strains, to improve the properties and effectively preserve them for brewing industry. In this study, 16 commercial nuruk samples were obtained from the commercial markets located in Chungcheong areas in Korea. 174 fungal strains were isolated from the samples on DG18 medium using a dilution plating method and then screened for enzyme activity and acid production. The active strains were identified based on the morphological characteristics and ITS sequence analysis. Out of 174 strains, 12 strains showed high amylase activity. Especially, Rhizopus sp. CN084, CN174, Aspergillus sp. CN161 and Mycocladus sp. CN042 showed high saccharogenic power and dextrinogenic enzyme activity on cooked wheat bran medium. On the other hand, Aspergillus sp. CN010, CN161, Rhizopus sp. CN105, CN168 and Rhizomucor sp. CN088 produced high acid production on the same medium. Our results showed that the active strains may be used as microbial sources for nuruk starter with good quality in brewing.

• Korean Journal of Food Science and Technology
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• v.29 no.3
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• pp.555-562
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• 1997

The characteristics of mash qualities of takju prepared by using different nuruk (Korean-style bran koji) such as Mucor racemosus nuruk, Rhizopus japonicus nuruk, Aspergillus oryzae nuruk, Aspergillus kawachii nuruk and traditional nuruk were investigated during fermentation. At the begining of fermentation, ethanol content was in the range of $2.0{\sim}3.0%$. However, it increased to $8.2{\sim}12.6%$ after 16 days of fermentation. Takju made from Rhizopus japonicus nuruk showed higher ethanol content than treated otherwise. pH of takju made from Rhizopus japonicus nuruk showed higher value the others. Total acids were $0.15{\sim}0.20%$ at the begining of fermentation, and it increased to $0.086{\sim}1.57%$ after 16 days of fermentation. Total sugar were $16.64{\sim}17.62%$ at the begining of fermentation, but decreased to below 7.00% after 16 days of fermentation. Rhizopus japonicus nuruk showed the lowest level of total sugar content. Except ethanol, iso-amyl alcohol and iso-butyl alcohol were major part of minor alcohol in the mash of takju. Higher concentration of iso-amyl alcohol, iso-butyl alcohol and n-propyl alcohol were found in the mash of Rhizopus japonicus nuruk whereas the level of phenylethyl alcohol was high in the mash of traditional nuruk. Fusel oil was $0.002{\sim}0.411\;mg/mL$ during fermentation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.2
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• pp.211-215
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• 2002

Pretense production and its characteristics were investigated for Rhizopus stolonifer, Rhizopus oryzae and Absidia corymbifera which were isolated from Korean traditional meju. The optimum culture conditions of the strains for the production of protease in basic medium [wheat bran : 1% glucose solution=1 : 1 (w/v)] were 3$0^{\circ}C$ and 4 days. The optimum pH and temperature for the enzyme activity of crude enzymes produced by Rhizopus sto-lonifer, Rhizopus oryzae and Absidia corymbifera were pH 6.0 and 5$0^{\circ}C$, respectively. The enzymes were relatively stable at pH 4.0~7.0, at temperature below 4$0^{\circ}C$, and at NaCl concentration lower than 16%. The $K_{m}$ value for Hammastein casein was 3.3$\times$10$^{-4}$ , 0.75$\times$10$^{-4}$ and 1.3$\times$10$^{-4}$ M, and $V_{max}$ value was 17.2$\mu\textrm{g}$/min, 9.4$\mu\textrm{g}$/min and 7.8$\mu\textrm{g}$/min, respectively.y.

• Korean Journal of Health Education and Promotion
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• v.15 no.1
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• pp.133-149
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• 1998

본 연구는 미국에 사는 한국인 이민자들의 암예방과 식품에 대한 지식과 신념에 관하여 알아보는데 그 목적이 있다. 본 연구의 도구로는 미국 국민 건강연구조사 (NHIS)의 설문지를 한국어로 번역하였고, 연구의 대상자는 미국의 시카고에 거주하는 263명의 한국인 이민자들이다. 본 연구의 주된 연구결과는 다음과 같았다. (1) 대부분의 대상자(83.3%)들은 한국음식을 먹고 있다고 답하였다. (2) 대상자들의 47.5%가 영어를 전혀 못 읽거나 거의 못 읽는다고 답하였다. (3) 식생활 변화를 하지 않는 이유에 대해 현재 먹고 있는 음식을 즐기기 때문에 식생활을 바꾸고 싶지 않다는 답이 가장 많았다. (4) 우리가 먹고 마시는 음식과 관계 있는 주요질병에 대해서 25.4%의 응답자만이 암이라고 답하였다 (미국 NHIS 에서는 48%). (5) 응답자의 48.3%만이 섬유소에 대해 들어보았다고 응답하였다. (6) 7.1%의 응답자만이 섬유소가 많은 음식으로 corn flakes와 bran flakes를 고를 수 있었다. 이 결과로 보아 한국인 이민자들이 미국음식에 대해 잘 모르는 것으로 보인다. (7) 약 62%의 응답자들이 암예방에 관한 무료 보건강좌에 참석하고 싶다고 응답하였다. 보건강좌 장소에 대해 "교회'라고 답한 응답자가 가장 많았다. (8) 질병예방에 관한 정보를 어디에서 얻느냐는 질문에 대하여 "신문"이라고 답한 응답자가 가장 많았다. 본 연구에서 얻은 결과를 볼 때 대부분의 응답자들이 미국 정부에서 하고 있는 암예방 교육의 혜택을 받고 있지 않는 것으로 나타났다. 한국인 이민자들의 문화적 배경에 맞는 암교육이 필요하며, 영어를 잘 못하는 이민자들을 위해 한국말로 번역된 보건교육자료를 사용하여야 한다.교육자료를 사용하여야 한다.표현을 위하여 확장된 기본 모델을 중심으로 각 레벨의 구성 요소들의 형식적 의미(formal semantics)와 레벨 내 혹은 레벨 구성요소들간의 관계성(relationship), 그리고 제약조건의 표현과 질의 추론 규칙들을 식별하여 FOPL(First Order Predicate Logic)로 표현한다. 또한, 본 논문은 FOPL로 표현된 predicate들과 규칙들을 구현하기 위하여 Prolog로 변환하기 위한 이론적 방법론을 제시하고 정보자원 관리를 위한 기본 함수들과 스키마 진화(schema evolution)를 위한 방법론을 제안한다. 최저 잔류탁도를 나타내는 최적 응집제 주입량에서의 Zeta potential은 원수탁도가 5NTU일 경우 Alum, PAC 및 PACS 모두 -20mV∼-15mV사이였으며, 원수 탁도가 10NTU인 경우에는 0∼0.5mV 범위에 있는 것으로 나타나 응집제 종류 및 주입량이 상이하더라도 응집효율이 가장 양호한 상태에서의 Zeta potential은 일정한 범위내에 있는 것으로 나타났다.각 각 11.1, 10.2, 12.2 그리고 13.0%의 발달율을 보여 유의적인 차이를 보이지 않았다. 4. 수정 후 114 시간 개별배양된 수정란으로부터 분리된 small과 large의 할구를 공핵체로 사용한 처리구에서 핵이식 수정란의 세포융합율에 있어서 각각 71.0, 71.4, 69.9 및 77.1% 의 융합율올 보여 유의적인 차이를 나타내지 않았으며, 핵이식 수정란의 배반포기배로의 발달율에 있어서도 각각 11.4%, 8.0%, 17.2% 그리고 12.9% 의 발달율을 보여 유의적인 차이를 보이지 않았다. 이상의 결과로 보아 핵이식 수정란을 효율적으로 생산하기 위하여 수핵난자의 세포질에 ionomycin 과 DMAP 의

• KSBB Journal
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• v.24 no.2
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• pp.163-169
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• 2009

Lovastatin (also known as Mevinolin, Mevacor, and Monacolin K), an inhibitor of the HMG-CoA reductase produced by Aspergillus terreus and other fungi, is used to reduce serum cholesterol levels in human beings. It is derived biosynthetically from two polyketides. One of these is a nonaketide that undergoes cyclization at a hexahydronaphthalene ring system, and the other is a simple diketide, 2-methylbutyrate. Two primer pairs were designed based on the amino acid sequences of lovastatin polyketide synthase and lovastatin diketide synthase for the PCR screening of lovastatin-producing strains. Among the seven selected strains, SJ-2 evidenced the highest level of lovastatin production in both liquid and solid cultures. Soybeans with SJ-2 were treated via 1 hour of heat shock at $30^{\circ}C$ for the mass production of lovastatin. The heat-treated soybeans were inoculated on rice bran and the koji extract was obtained after 15 days of incubation. It yielded the highest level of lovastatin production among the strains, and also evidenced 75% inhibition activity against HMG-CoA reductase. We developed an efficient PCR screening method for lovastatin-producing strains, using lovastatin biosynthesis genes.

• Food Science and Preservation
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• v.23 no.5
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• pp.738-745
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• 2016

We evaluated the distribution pattern of tannin in 164 sorghum breeding lines and the inhibition rates of amylase, protease, and lipase in sorghum lines with different tannin concentrations. Tannin was existed in the testa of sorghum grain. The tannin content in whole grain of Nampungchal sorghum was 11.54 mg/g, and that in grain (milling rate 73%) and bran fractions was 4.57 mg/g and 28.71 mg/g, respectively. The inhibition rate of ${\alpha}$-amylase, ${\alpha}$-glucosidase, and ${\beta}$-glucosidase in sorghum lines with tannin was higher than that in sorghum lines without tannin. The inhibition rate of ${\alpha}$-glucosidase was greater than 97% in sorghum lines with tannin. The inhibition rate of protease ranged from 20% to 70% in the sorghum lines, showing no discernable trends in tannin content. Lipase inhibition was either very low or not observed and did not seem to correlate with tannin concentration.

• Journal of the East Asian Society of Dietary Life
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• v.18 no.3
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• pp.331-336
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• 2008

We investigated the processing aptitude of rice embryo powder as covering and stuffing in rice cake. The quality characteristics of gelatin jelly with rice embryo powder as a stuffing was also examined. Covering steamed rice cake with 50% rice embryo led to less crumbling compared to that of 100% rice embryo powder. Jelly with rice embryo powder was not significantly different with control group in appearance(p>0.05). Covering steamed rice cake with 50% rice embryo powder was not significantly different compared to 100% soybean in color, flavor, taste, toughness, and overall acceptability(p>0.05). Furthermore, there was no significant difference between sesame and rice embryo stuffing in sensory evaluation(p>0.05). When 5% rice embryo powder was added to gelatin jelly, hardness, springiness, and gumminess was not different to that of control(p>0.05), while chewiness did increase(p<0.05). Jelly added with 5% rice embryo had the highest taste acceptability.

• Korean Journal of Agricultural Science
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• v.2 no.1
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• pp.241-255
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• 1975

This experiment was conducted to observe the effects of fermented feed by Aspergillus oryzae and Aspergillus niger on the improvement of feed value and the effect of fermented feed additive for meat production of broiler. The results of fermented feed on the improvement of feed value were as follows; I; The effects of fermented feed value improvement were as follaws; 1) There were little difference between fermented feeds by Asp. oryzae and Asp. niger, compared with wheat bran, crude protein contents of Koji was highly increased and its nitrogen free extract and crude fat contents were decreased, but crude fiber and ash were little difference. 2) Total amino acids were highly increased as to fermented feeds but proline in Asp. niger koji feed, and proline and valine in Asp. oryzae koji feed were decreased and other amino acid were increased 2) The effect of fermented feeds on meat production of broiler were as follows; 1) Fermented feeds groups appeared higher weight (p<0.01)than weight of control on end of experimental period, but little difference were recognized between 5% and 10% fermented feed groups. 2) On the weight gain per day, highly significant were recognized(p<0.05) between control and test groups, 10% Asp. oryzae koji group was highest ($12.15{\pm}0.46g$) between all groups. 3) On the yield of carcass, there were significant highly difference (p<0.01) between control and test groups but little difference were recognized between each of 5% groups and 10% groups of fermented feeds. 4) Fermented feed groups appeared higher carcass yield (p (0<0.05) than control. But between all fermented feed groups were a little difference in partly. 5) On the influence of fowl meat composition, amount of moisture contents was a little decrease in fermented feed groups, and crude protein and crude fat were increased. 6) Feed conversion rate resulted a little amount decreasing. Specially, 10% Asp oryzae koji group was lowest (2.89) compare with control (3.35) 3. As a result of economical analysis appeared highest low income in koji groups. Low income were more gained percent of 40.22 in 10% Asp oryzae koji and 33.19 in 10% Asp. niger koji than control.

• The Korean Journal of Mycology
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• v.31 no.3
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• pp.200-205
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• 2003

The study was carried out on the artificial cultivation of the abalone mushroom, Pleurotus cystidiosus O.K.Miller. The pine sawdust substrates with 20% rise bran were good for mycelial growth and high quantity of P. cystidiosus in the bottle cultivation. Moreover, the proper volume for bottle cultivation was 850 ml and the removal of spawn and surface layer of the medium before pin-heading was more efficient. The yields of P. cystidiosus were higher in sawdust substrates added calcium carbonate than those not added calcium carbonate. The volume of 3 kg polypropylene bag is good for yield and biological efficiency in bag cultivation of P. cystidiosus. Cotton wastes were proper substrates for bag cultivation. In the effect of different cultivation temperature, $28{\pm}2^{\circ}C$ cultivation temperature was good for for primordial formation after inoculation.