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Effects of L-carnitine, Selenium-enriched Yeast, Jujube Fruit and Hwangto (Red Clay) Supplementation on Performance and Carcass Measurements of Finishing Pigs

  • Han, Yung-Keun;Thacker, P.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.2
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    • pp.217-223
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    • 2006
  • Fifty castrated crossbred ($Landrace{\times}Yorkshire$) pigs, weighing an average of $60.6{\pm}3.1kg$ were allotted to one of five treatments in a randomized block design to examine the effects of dietary inclusion of 0.1% L-carnitine (50 ppm carnitine), 0.1% selenium-enriched yeast (0.3 ppm selenium), 0.1% Jujube fruit or 0.1% Hwangto (Red clay) on pig performance and carcass quality. All diets were based on corn, wheat, soybean meal and wheat bran and were formulated to supply 13.8 MJ DE/kg. Dietary supplementation did not influence daily gain (p = 0.57), feed intake (p = 0.52), or feed conversion (p = 0.32). Digestibility of dry matter (p = 0.60), organic matter (p = 0.74), crude protein (p = 0.76), crude fibre (p = 0.70) and energy (p = 0.75) were also unaffected by inclusion of any of the additives. Tissue samples taken from the longissimus muscle showed that the levels of carnitine (p = 0.0001) and selenium (p = 0.0001) were significantly higher with dietary inclusion of carnitine or selenium-enriched yeast. Dietary treatment did not affect dressing percentage (p = 0.33), carcass lean yield (p = 0.99) or first, $10^{th}$ and last rib midline backfat depth (p = 0.45, 0.82 and 0.47, respectively). Dietary treatment also did not affect the percentages of tenderloin (p = 0.37), bacon (p = 0.36), fat and bone (p = 0.56), picnic shoulder (p = 0.25), skirt (p = 0.80), fresh ham (p = 0.31) or ribs (p = 0.79). However, pigs fed the diet containing Jujube fruit had a higher percentage of Boston butt than pigs fed the carnitine or selenium supplemented diets (p = 0.01). Pigs fed added Hwangto had a higher (p = 0.04) percentage of loin compared with pigs fed supplementary selenium or Jujube fruit. Loin muscle from pigs fed carnitine had a significantly lower Hunter colour value for L (whiteness, p = 0.004) and a higher value for $a^*$ (redness; p = 0.069). The overall results indicate that supplementation with L-carnitine and selenium-enriched yeast can produce pork containing higher levels of carnitine and selenium, which could provide health benefits for consumers of pork without detrimental effects on pig performance.

Interspecific Hybridization between Pleurotus ostreatus and Pleurotus sajor-caju by Protoplast Fusion (원형질체(原形質體) 융합(融合)에 의한 느타리와 여름느타리버섯의 종간(種間) 교잡(交雜))

  • Yoo, Young-Bok;Lee, Haing-Sook
    • The Korean Journal of Mycology
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    • v.22 no.4
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    • pp.378-385
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    • 1994
  • Interspecific somatic hybrids were obtained by protoplast fusion between Pleurotus ostreatus and Pleurotus sajor-caju. The fusion products between incompatible strains did not form clamp connections. Fruiting body of the clampless fusants was induced by light-dark cycle on saw-dust-rice bran substrate in glass bottles. Out of them, seven somatic hybrids produced fruiting bodies of intermediate morphology of the two species. Light and low temperature were the initiating factors for the development of clamped hyphae from the clampless mycelial colonies. All of these basidiocarps had clamp connections. Eight fusants from the six crosses were analysed with the segregation of genetic characters by random spore isolates. In the three combinations, unexpected alleles were shown. Somatic hybrid between P188 (P. ostreatus 2-1 + P. sajor-caju 2-53) and P. florida 2-3 by triple cross produced fruiting bodies similar to those of fusant between P. ostreatus and P. florida. All the genetic charaters from the three strains were shown to segregate and recombine.

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Decomposition of Fiber by the Basidiomycetes (담자균류(擔子菌類)에 의(依)한 섬유질류(纖維質類) 분해(分解)에 관(關)한 연구(硏究))

  • Kim, Sam-Soon;Kim, Ki-Ju
    • The Korean Journal of Mycology
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    • v.3 no.2
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    • pp.1-6
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    • 1975
  • Various enzymes produced by the Basidiomycetes, i.e. Pleurotus ostreatus, Pholiota nameko and Flammulina velutipes have been analysed. The purpose of the analyses is to produce a nutritious fertilizer from saw dust, sweet potato stems, corn stems and barley stems which are known as the farm product waste materials. The results of the analyses are as follows: 1. In order for P. ostreatus, Pho. nameko and F. velutipes to grow properly, the saw dust of broad-leaved trees, poplar and chestnut were mixed with sweet-potato stems, corn stems and barley straws at the ratio of $42{\sim}49\;:\;30$, and then to this mixture was added rice bran at the ratio of $18{\sim}35\;:\;30$ The following proved to be optimum conditions for the growth in this test: the parcentage of water content $60{\sim}70%$, $24{\sim}26^{\circ}C$ degrees of temperature. 2. We analyzed the fiber contained in the above materials after 20 days growth in various media. We found out that the fiber was decomposed up to $47{\sim}50%$ and polysaccharified. Lignin was decomposed up to $20{\sim}25%$ and about three times as much protein was contained in the product as in the original materials. 3. Our analyses revealed the possibility of producing concentrated feed from the crude fiber.

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Studies on immobilization and application of beta-galactosidase I. Conditions for production and properties of the enzyme from Aspergillus niger CAD 1 (beta-Galactosidase의 고정화 및 응용에 관한 연구 제1보: Aspergillus niger CAD 1의 효소생산 조건 및 효소학적 성질)

  • Lee, Yong-Kyu;Chun, Soon-Bae;Choi, Won-Ki;Chung, Ki-Chul;Bae, Suk;Kim, Kwan-Chun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.15 no.4
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    • pp.32-39
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    • 1986
  • A strain of Aspergillus niger CAD 1 which produces considerable amount of beta-galactosidase was selected from extracellular beta-galctaosidase producing fungi isolated from soil. Optimal conditions for the enzyme from Aspergillus niger CAD 1 were the growth in wheat bran supplemented with 0.5% skim milk powder at $30^{\circ}C$ for 72 hrs. The crude enzyme was purified 1,387 fold through DEAE-cellulosc and Sephadex G-100 chromatographr and its recovery was 6.2%, The optimal pH and temperature for the purified enzyme were pH 4.5 ana $45^{\circ}C$, respectively. The Km and Vmax on ONPG were $3.57{\times}10^3M$ and 33.0 unit/mg protein, whereas those on lacose were $83.3{\times}10^3M$and 15.33 unit/mg protein, respectively, The activation energy for the enzyme was 9,900 cal/mol and the enzyme had no metal ion requirement for its activity and stability. The hydrolysis of lactose in skim milk, 4.8% lactose solution and acidic whey were 65%, 70% and 78% after 10 hrs incubation at $45^{\circ}C$, when 182 units of the enzyme were used 50ml of the substrate solutions.

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Studies on the Bio-degradability and Characteristics of Mulching Films Containing Rice By-products Applied to Upland Crops (벼 부산물을 함유한 생분해성 멀칭비닐의 포장 재배조건에서의 특성 및 분해력 연구)

  • Han, Sang-Ik;Kang, Hang-Won;Jang, Ki-Chang;Seo, Woo-Duck;Oh, Seong-Hwan;Ra, Ji-Eun;Lee, Hyeong-Un;Chung, Mi-Nam;Choi, Kyung-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.2
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    • pp.99-105
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    • 2012
  • The main challenges for the development of agricultural bio-degradable mulching film concern the degradation during the lifetime of cultivated crops. A set of rice by-product (rice-hull and rice-bran) based bio-degradable mulching films was developed and tested, following the measurement of standard bio-degradability rate and adaptability in a large scale field experiment. The standard bio-degradability of bio-film passed the KS (Korea standard) regulation. The result of mechanical analysis of bio-degradable mulching film presented a higher mechanical strength and elongation rate compared with polyethylene film. In addition, bio-film could be degraded into fragments within 4 months under the field condition of several upland crops. Bio-degradable mulching film indicated great potential as a new source of agricultural bio-degradable material.

Studies for the Processing Factors of Pesticides during the Milling of Wheat Grain (밀의 제분에 따른 밀가루 중 농약 가공계수 산출 연구)

  • Park, So-Young;Park, Kun-Sang;Im, Moo-Hyeog;Choi, Hoon;Chang, Moon-Ik;Kwon, Chan-Hyeok;Kim, Sun-Gu;Lee, Hyo-Ku;Hong, Moo-Ki;Shim, Jae-Han;Kim, Jeong-Han
    • The Korean Journal of Pesticide Science
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    • v.13 no.2
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    • pp.70-78
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    • 2009
  • Processing factors of pesticides in milling process of wheat grain, which are consumed and imported at large quantity were examined to establish reasonable MRL of the processed food. Azinphos-methyl, chlorpyrifos, chlorpyrifos-methyl, fenitrothion, malathion, and trichlorfon were selected for the study according to annual usage and the previous detection record in wheat grain. Dipping process for pesticide application was performed in laboratory, while milling process was conducted under pilot plant system. Processing factors were calculated by analyzing residual pesticides on wheat grain and processing products as wheat flour, bran and red dog. Processing factors were 0.05 for azinphos-methyl, 0.06 for chlorpyrifos, 0.05 for chlorpyrifos-methyl, 0.07 for fenitrothion, 0.07 for malathion, 0.06 for trichlorfon, respectively. Recovery test was also performed to establish extraction efficiency of analytical procedure. The recovery value ranged from 93.2% to 98.6% with standard deviation of 0.1-0.9%.

PPL of Visual Media Affects Clothing Purchase of College Students (영상매체의 PPL이 의복 구매에 미치는 영향에 대하여)

  • Song, Jae-Wook;Na, Young-Joo
    • Science of Emotion and Sensibility
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    • v.9 no.4
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    • pp.331-339
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    • 2006
  • PPL(Product Placement) of visual media can change the purchasing behavior of College students. This study aimed to investigate the differences in consumer's conception about PPL among college students and to analyze the relationship between the PPL conception ant fashion innovation/fashion sensitivity of students, resulting into their real purchasing behavior. We surveyed to about 189 college students living near Seoul, with questionnaire about PPL conception, purchase of PPL clothing, satisfaction on PPL clothing, fashion innovation, opinion about PPL, PPL apparel brand to recall, etc. According to dichotomy of college students by fashion innovation, as higher fashion innovative they were, they paid more attention on PPL clothing while watching TV or movie, and they also could recall more PPL apparel brands, and they purchased more PPL clothing. While the students with low fashion innovation had references from the opinion of friends or around people when purchasing their clothing, the students with high fashion innovation did from fashion magazine or PPL clothing. The students who thought that PPL was important and effective on clothing purchasing behavior, answered that their preference to PPL bran4, to the company, and to the product had increased positively, but not to the actor/actress.

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Vanillic Acid Stimulates Anagen Signaling via the PI3K/Akt/β-Catenin Pathway in Dermal Papilla Cells

  • Kang, Jung-Il;Choi, Youn Kyung;Koh, Young-Sang;Hyun, Jin-Won;Kang, Ji-Hoon;Lee, Kwang Sik;Lee, Chun Mong;Yoo, Eun-Sook;Kang, Hee-Kyoung
    • Biomolecules & Therapeutics
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    • v.28 no.4
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    • pp.354-360
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    • 2020
  • The hair cycle (anagen, catagen, and telogen) is regulated by the interaction between mesenchymal cells and epithelial cells in the hair follicles. The proliferation of dermal papilla cells (DPCs), mesenchymal-derived fibroblasts, has emerged as a target for the regulation of the hair cycle. Here, we show that vanillic acid, a phenolic acid from wheat bran, promotes the proliferation of DPCs via a PI3K/Akt/Wnt/β-catenin dependent mechanism. Vanillic acid promoted the proliferation of DPCs, accompanied by increased levels of cell-cycle proteins cyclin D1, CDK6, and Cdc2 p34. Vanillic acid also increased the levels of phospho(ser473)-Akt, phospho(ser780)-pRB, and phospho(thr37/46)-4EBP1 in a time-dependent manner. Wortmannin, an inhibitor of the PI3K/Akt pathway, attenuated the vanillic acid-mediated proliferation of DPCs. Vanillic acid-induced progression of the cell-cycle was also suppressed by wortmannin. Moreover, vanillic acid increased the levels of Wnt/β-catenin proteins, such as phospho(ser9)-glycogen synthase kinase-3β, phospho(ser552)-β-catenin, and phospho(ser675)-β-catenin. We found that vanillic acid increased the levels of cyclin D1 and Cox-2, which are target genes of β-catenin, and these changes were inhibited by wortmannin. To investigate whether vanillic acid affects the downregulation of β-catenin by dihydrotestosterone (DHT), implicated in the development of androgenetic alopecia, DPCs were stimulated with DHT in the presence and absence of vanillic acid for 24 h. Western blotting and confocal microscopy analyses showed that the decreased level of β-catenin after the incubation with DHT was reversed by vanillic acid. These results suggest that vanillic acid could stimulate anagen and alleviate hair loss by activating the PI3K/Akt and Wnt/β-catenin pathways in DPCs.

Effects of Organic Liquid Fertilizer Fertigation on Growth and Fruit Quality of Hot Pepper (유기액비 관비가 고추 생육 및 과실 품질에 미치는 영향)

  • Joo, Seon-Jong;Lee, Guang-Jae
    • Korean Journal of Organic Agriculture
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    • v.18 no.1
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    • pp.63-74
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    • 2010
  • The present study was carried out to evaluate the effects of organic liquid fertilizer (OLFs) fertigation on the growth and fruit quality of hot pepper (Capsicum annuum L.) cv. 'Nokkwang (Seminis Korea)'. Treatments were given with control (chemical feritilzer), OLF I(fowl dropping : fish meal : soybean meal, 2:1:1), OLF II(fowl dropping : bone meal : soybean meal, 2:1:1), OLF III(fowl dropping : bone meal, 1:1), and OLF IV(fowl dropping : rice bran : soybean meal, 2:1:1). The composition ratio of OLF based on weight to weight. The pH and EC of OLFs according to different material and compositions ranged from 6.5 to 6.8, and from 2.91 to $3.69dS{\cdot}m^{-1}$, respectively. The C/N ratio was the highest as 5.75 in OLF Ⅳ, and there was no difference among the treatments in C/N ratio of the exception of OLF IV. In OLF IV total fruit weight was the highest as 1,558g per plant and total fruit number increased as 23.7% compared to control. The content of capsaicin in the ripened pepper increased with OLF I and II treatments, and decreased with OLF III and IV treatments compared to control of $662mg{\cdot}kg^{-1}$, but the soluble solid content was the highest with OLF IV treatment as $10.0^{\circ}Brix$.

Isolation of Microorganisms and Development of Microbial Augmentation for Treatment of Industrial Wastewater containing Ammonium Nitrogen (암모니아성 질소함유 산업폐수처리를 위한 미생물의 분리 및 복합미생물제제의 개발)

  • Lee, Myoung-Eun;Mun, Seo-Jin;Kwon, Do-Hyuck;Suh, Hyun-Hyo
    • Journal of Life Science
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    • v.30 no.2
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    • pp.129-136
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    • 2020
  • For effective treatment of wastewater containing ammonium nitrogen (NH4-N), AT2, AT9, and AT12 strains, having high total organic carbon (TOC) removal capability, and FN47, possessing excellent ammonia nitrogen removal capability present in the activated sludge in the aeration tank of food wastewater treatment plants, were isolated and identified. The cells of these isolated strains were used for microbial augmentation with FIW-1 in the defatted rice bran as a medium to treat industrial wastewater. The investigation of the cultural characteristics of these isolated strains in the aeration tank showed that the affinities for substrate of the isolated strains were extremely high, of which AT12 (Alcaligenes sp. AT12) was the highest among the isolated strains. Ammonium nitrogen removal efficiency in the food wastewater was 71% in the isolated strain FN47 (Microbacterium sp. FN47) treatment group. When only activated sludge was added in the lab scale pilot using food wastewater during continuous culture experiment, the TOC removal efficiency was 63%. Meanwhile, the removal efficiency of 92% was obtained when the microbial augmentation FIW-1 for wastewater treatment was applied. In addition, the chemical oxygen demand (COD) level from the effluent wherein microbial augmentation FIW-1 was input for the initial three days in the wastewater treatment site experiment showed a treatment rate of about 43%, which was increased to 62% after an elapse of 5 days.