• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.273-273
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• 2013

Recently, Point-of-care (POC) testing microdevices enable to do the patient monitoring, drug screening, pathogen detection in the outside of hospital. Immunochromatographic strip (ICS) is one of the diagnostic technologies which are widely applied to POC detection. Relatively low cost, simplicity to use, easy interpretations of the diagnostic results and high stability under any circumstances are representative advantages of POC diagnosis. It would provide colorimetric results more conveniently, if the genetic analysis microsystem incorporates the ICS as a detector part. In this work, we develop a reverse transcriptase-polymerase chain reaction (RT-PCR) microfluidic device integrated with a ROSGENE strip for colorimetric influenza H1N1 virus detection. The integrated RT-PCR- ROSGENE device is consist of four functional units which are a pneumatic micropump for sample loading, 2 ${\mu}L$ volume RT-PCR chamber for target gene amplification, a resistance temperature detector (RTD) electrode for temperature control, and a ROSGENE strip for target gene detection. The device was fabricated by combining four layers: First wafer is for RTD microfabrication, the second wafer is for PCR chamber at the bottom and micropump channel on the top, the third is the monolithic PDMS, and the fourth is the manifold for micropump operation. The RT-PCR was performed with subtype specific forward and reverse primers which were labeled with Texas-red, serving as a fluorescent hapten. A biotin-dUTP was used to insert biotin moieties in the PCR amplicons, during the RT-PCR. The RT-PCR amplicons were loaded in the sample application area, and they were conjugated with Au NP-labeled hapten-antibody. The test band embedded with streptavidins captures the biotin labeled amplicons and we can see violet colorimetric signals if the target gene was amplified with the control line. The off-chip RT-PCR amplicons of the influenza H1N1 virus were analyzed with a ROSGENE strip in comparison with an agarose gel electrophoresis. The intensities of test line was proportional to the template quantity and the detection sensitivity of the strip was better than that of the agarose gel. The test band of the ROSGENE strip could be observed with only 10 copies of a RNA template by the naked eyes. For the on-chip RT-PCR-ROSGENE experiments, a RT-PCR cocktail was injected into the chamber from the inlet reservoir to the waste outlet by the micro-pump actuation. After filling without bubbles inside the chamber, a RT-PCR thermal cycling was executed for 2 hours with all the microvalves closed to isolate the PCR chamber. After thermal cycling, the RT-PCR product was delivered to the attached ROSGENE strip through the outlet reservoir. After dropping 40 ${\mu}L$ of an eluant buffer at the end of the strip, the violet test line was detected as a H1N1 virus indicator, while the negative experiment only revealed a control line and while the positive experiment a control and a test line was appeared.

• Geomechanics and Engineering
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• v.32 no.1
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• pp.111-123
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• 2023

The slope of an open cut tunnel is located above the exit of the Leijia tunnel on the Changgan high-speed railway. During the excavation of the open cut tunnel foundation pit, the slope slipped twice, a large landslide of 92500 m³ formed. The landslide body and unstable slope body not only caused the foundation pit of the open cut tunnel to be buried and the anchor piles to be damaged but also directly threatened the operational safety of the later high-speed railway. Therefore, to study the stability change in the slope of the open cut tunnel under heavy rain and excavation conditions, a 3D numerical calculation model of the slope is carried out by Midas GTS software, the deformation mechanism is analyzed, anti-sliding measures are proposed, and the effectiveness of the anti-sliding measures is analyzed according to the field monitoring results. The results show that when rainfall occurs, rainwater collects in the open cut tunnel area, resulting in a transient saturation zone on the slope on the right side of the open cut tunnel, which reduces the shear strength of the slope soil; the excavation at the slope toe reduces the anti-sliding capacity of the slope toe. Under the combined action of excavation and rainfall, when the soil above the top of the anchor pile is excavated, two potential sliding surfaces are bounded by the top of the excavation area, and the shear outlet is located at the top of the anchor pile. After the excavation of the open cut tunnel, the potential sliding surface is mainly concentrated at the lower part of the downhill area, and the shear outlet moves down to the bottom of the open cut tunnel. Based on the deformation characteristics and the failure mechanism of the landslides, comprehensive control measures, including interim emergency mitigation measures and long-term mitigation measures, are proposed. The field monitoring results further verify the accuracy of the anti-sliding mechanism analysis and the effectiveness of anti-sliding measures.

• Journal of Environmental Impact Assessment
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• v.32 no.6
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• pp.473-487
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• 2023

Korea's multi-purpose dams, which were constructed in the 1970s and 1980s, have a single outlet located near the bottom for hydropower generation. Problems such as freezing damage to crops due to cold water discharge and an increase the foggy days have been raised downstream of some dams. In this study, we analyzed the effect of water intake depth on the reservoir's water temperature stratification structure and outflow temperature targeting Hapcheon Reservoir, where hypolimnetic withdrawal is drawn via a fixed depth outlet. Using AEM3D, a three-dimensional hydrodynamic water quality model, the vertical water temperature distribution of Hapcheon Reservoir was reproduced and the seasonal water temperature stratification structure was analyzed. Simulation periods were wet and dry year to compare and analyze changes in water temperature stratification according to hydrological conditions. In addition, by applying the intake depth change scenario, the effect of water intake depth on the thermal structure was analyzed. As a result of the simulation, it was analyzed that if the hypolimnetic withdrawal is changed to epilimnetic withdrawal, the formation location of the thermocline will decrease by 6.5 m in the wet year and 6.8 m in the dry year, resulting in a shallower water depth. Additionally, the water stability indices, Schmidt Stability Index (SSI) and Buoyancy frequency (N²), were found to increase, resulting in an increase in thermal stratification strength. Changing higher withdrawal elevations, the annual average discharge water temperature increases by 3.5℃ in the wet year and by 5.0℃ in the dry year, which reduces the influence of the downstream river. However, the volume of the low-water temperature layer and the strength of the water temperature stratification within the lake increase, so the water intake depth is a major factor in dam operation for future water quality management.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.6
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• pp.330-335
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• 2017

The demand for electric motor fuel cells has surged in the automotive industry, leading to a recent increase in the demand for square aluminum cans used as fuel cell battery casings. The air vent located on the bottom of the rectangular battery casing prevents large explosions by intermittent pressure release prior to the accumulation of abnormally high pressures. Conventionally, the square cup battery casing is produced via six-step deep drawing, with the outer shape of the vent being manufactured by welding to the square battery casing. On the other hand, this study directly incorporated the air vent outlet into the bottom surface of the rectangular casing. The product of a coupled finite element analysis technique applying the thickness and contour generated from the square cup multi-step deep drawing formation analysis was used as the forging input shape. The results yielded increased prediction accuracy and the advanced prediction of defects, such as swelling and fracture. Based on the results of the initial analyses, two of the generated forging shapes were determined to be suitable, with the optimal forging shape being determined by molding analysis. The results presented here were validated by mold fabrication and a subsequent comparison of the actual and analytical results.

• Korean Journal of Ecology and Environment
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• v.41 no.3
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• pp.360-366
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• 2008

The spatial and temporal variations of the high turbid water by a single event of heavy rain (total 299.1 mm and daily maximum 99.4 mm) were studied in Andong Reservoir, which has hypolimnetic discharges. Turbid water entered into the reservoir, was isolated from the bottom at the midreservoir and then passed through the metalimnion as an interflow current in the lacustrine zone. Maximum turbidity was 290 NTU at 16 m depth of the midreservoir, but the initial turbidity showed about 10 NTU in the reservoir before the rainfall. Turbid water in the reservoir affected to increase the withdrawal turbidity from the 3rd day after the rainfall, the maximum turbidity was 129 NTU at 5th day after the rainfall. Turbid water that flew towards the downreservoir distributed within 5 m above the outlet gate of the intake tower, showing the maximum turbidity, and that was decreased in its thickness and concentration by discharging through the intake tower. It has taken 38 days until the turbidity in the withdrawal reduced to 30 NTU, and 87 days to reduce the turbidity to the way when it was before the rainfall, with the correlation coefficient of 0.96 and 0.97, respectively. Turbid water was withdrawn from the reservoir by entraining into the intake tower as a form of the interflow, and not be settled down to the bottom of the reservoir. Therefore, we assessed that the depth of the withdrawal was appropriately positioned in Andong Reservoir, so as to withdraw the turbid water effectively from the reservoir.

• Korean Journal of Ecology and Environment
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• v.38 no.1 s.110
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• pp.105-117
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• 2005

During the period of heavy rain from 2002 to 2004, the characteristics of the inflow, temporal and spatial fluctuations of high turbid water according to thermal stratification were studied on the Andong Reservoir which is the largest artificial lake in the Nakdong River basin, Korea. Thermal stratification was formed in June. Its structure determined to the pathway of inflowing turbid water and has affected by the transportation of high turbid water. Regardless of the time and amount of inflow, the high turbid water showed the shape of underflow at the riverine zone, separated from the bottom at the transition zone and moved to the lacustrine zone with the shape of density current. The plunging point depended on the time and amount of inflow. The distributions of thermal stratification and DO concentrations were changed by inflowing discharge. Two thermoclines and minimum DO layers were found out existing at metalimnion in a specific time, respectively. The layer of high turbid water which formed with the thickness of 20 m at the maximum below the depth of 15 m moved toward dam. Not settled to the bottom, the newly formed layer was discharged through the intake-outlet and dispersed into all layers by the circulation in the fall.

• Journal of Korean Society of Water and Wastewater
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• v.19 no.4
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• pp.421-428
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• 2005

Dissolved air flotation (OAF) is a solid-liquid separation system that uses fine bubbles rising from bottom to remove particles in water. In this study, we investigated the effect of L/W (L; Length, W; Width) on the hydro-dynamic behavior in DAF system using CFD (Computational Fluid Dynamics) and ADV (Acoustic Doppler Velocimetry) technique. The factual full-scale DAF system, L/W ratio of 1:1, was selected and various L/W ratio (2:1, 3:1, 4:1 and 5:1) conditions were simulated with CFD. For modelling, 2-phase (gas-liquid) flow equations for the conservation of mass, momentum and turbulence quantities were solved using an Eulerian-Eulerian approach based on the assumption that very small particle is applied in the DAF system. Also, for verification of CFD simulation results, we measured the factual velocity at some points in the full-scale DAF system with ADV technique. Both the simulation and the measurement results were in good accordance with each other. As the results of this study, we concluded that L/W ratio and outlet geometry play important role for flow pattern and fine bubble distribution in the flotation zone. In the ratio of 1:1, the dead zone is less than those in other cases. On the other hands, in the ration of 3:1, the fine bubbles were more evenly distributed.

• Korean Chemical Engineering Research
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• v.50 no.4
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• pp.738-742
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• 2012

Here, we develop a new nanofilter device for the rapid and efficient separation of nanoparticles and biomolecules, exploiting the use of AAO mebrane with ordered nanopores in the range from 20 nm to 200 nm. Briefly, the chip comprises of a series of the upper and lower PDMS channels containing embedded inlet and outlet ports, and $50{\mu}m$ width microfluidic channel, and AAO membrane to be made the filtering zone. After assembling these components, the acrylate plastic plates were used to fix the device on the top and bottom side. When introducing the samples into the inlet ports of the upper PDMS channel, we were able to separate and concentrate the nanoparticles and target molecules at the filtering zone, and to elute the solutions containing the unwanted materials toward the lower PDMS channels normal to the direction of AAO membrane. To demonstrate the usefulness of the device we apply it to the SERS detection of nucleic acid sequences associated with Dengue virus serotype 2. We report a limit of detection for Dengue sequences of 300 nM and show excellent enhancement of Raman signals from the filter zone of the nanofilter device.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.7 no.1
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• pp.1-8
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• 1971

The forth dye diffusion experiment, which was authorized by the Office of Atomic Energy, and some physical aspect of oceanographic observations were carried out in the sea off Kori in September 29-October 1, 1971. In a series of drift bottle experiments the 337 bottles were released. And bottom topography by echo soundings in the survey was well approached to the chart No. 433, Kori and Approaches, published by Hydrographic Office in October 1970. Results deduced from this survey were as follows: 1. A small ridge extends from Kori-Mal tip in southwestward over 1300 meters, and eastside of which is plain and a little sloping while westside of which cliffy and very steeper sloping. 2. A gyre was formed in the waters from Kori-Mal to Mat-dum in cum sole definitely at flood tide and in contra solem less definitely at ebb tide. And that the gyre rose and decayed with the turn of the tide. 3. Diffusion coefficients of 2.5% Rhodamine B solution of 200 liters were 9. 3 ${\times}{10^2}$ c$m^2$/sec in the waters Kori-Mal to Mat-dum and 28.6${\times}{10^2}$ c$m^2$/sec in the waters eastside of Mat-dum at the time after 26 minutes from releasing of the dye solution. 4. According to the above results the eastside of Mat-dum is rather recommendable to settle as the point for the outlet of drainage or waste of atomic power plant to be constructed.

• 한국해양학회지
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• v.11 no.1
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• pp.25-33
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• 1976

The physical factors and chemical contents were studied at 8 stations within Masan Bay, differing in depth and location with respect to inner and outer harbours. It is a relatively small bay with a long and narrow outlet. The water temperature and salinity at the inner bay was rather subject to change on weather condition of the neighbouring land than on the effects of the water mass of outer bay. The high nutrient contents in the bay were caused by the sewage and industrial activities. The outer harbour was less polluted than that of the inner bay; the high contents were significantly decreased from the entrance of the outer harbour. While the high disolved oxygen contents, over saturation in the surface strata may be the result of nearly all year round phytoplankton blooms, the lack of oxygen contents in the bottom strata were caused by the inadequate mixing of water mass and organic matters. The frequent red tide in the area may be the results of inflowing raw sewage, industrial activities of neighbouring land and inadequate mixing of water masses.