• Journal of Microbiology and Biotechnology
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• 제24권8호
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• pp.1034-1043
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• 2014

This study determined the effect of light intensity and photoperiod on the dry cell weight and total amount of carotenoids in four isolates of purple non-sulfur bacteria obtained from shaded and exposed microhabitats of a mangrove ecosystem in Kota Kinabalu, Sabah, Malaysia. The initial isolation of the bacteria was carried out using synthetic 112 medium under anaerobic conditions (2.5 klx) at $30{\pm}2^{\circ}C$. On the basis of colony appearance, cell morphology, gram staining, motility test, and 16S rRNA gene sequencing analyses, all four bacteria were identified as Afifella marina. One of the bacterial isolates, designated as Af. marina strain ME, which was extracted from an exposed mud habitat within the mangrove ecosystem, showed the highest yield in dry cell weight ($4.32{\pm}0.03g/l$) as well as total carotenoids ($0.783{\pm}0.002mg/g$ dry cell weight). These values were significantly higher than those for dry cell weight ($3.77{\pm}0.02g/l$) and total carotenoid content ($0.706{\pm}0.008mg/g$) produced by the isolates from shaded habitats. Further analysis of the effect of 10 levels of light intensity on the growth characteristics of Af. marina strain ME showed that the optimum production of dry cell weight and total carotenoids was achieved at different light intensities and incubation periods. The bacterium produced the highest dry cell weight of 4.98 g/l at 3 klx in 72 h incubation, but the carotenoid production of 0.783 mg/g was achieved at 2.5 klx in 48 h incubation. Subsequent analysis of the effect of photoperiod on the production of dry cell weight and total carotenoids at optimum light intensities (3 and 2.5 klx, respectively) revealed that 18 and 24 h were the optimum photoperiods for the production of dry cell weight and total carotenoids, respectively. The unique growth characteristics of the Af. marina strain ME can be exploited for biotechnology applications.

• Parasites, Hosts and Diseases
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• 제57권4호
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• pp.445-450
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• 2019

Human infections due to the monkey malaria parasite Plasmodium knowlesi is increasingly being reported from most Southeast Asian countries specifically Malaysia. The parasite causes severe and fatal malaria thus there is a need for urgent measures for its control. In this study, the level of polymorphisms, haplotypes and natural selection of full-length pkmsp8 in 37 clinical samples from Malaysian Borneo along with 6 lab-adapted strains were investigated. Low levels of polymorphism were observed across the full-length gene, the double epidermal growth factor (EGF) domains were mostly conserved, and non-synonymous substitutions were absent. Evidence of strong negative selection pressure in the non-EGF regions were found indicating functional constrains acting at different domains. Phylogenetic haplotype network analysis identified shared haplotypes and indicated geographical clustering of samples originating from Peninsular Malaysia and Malaysian Borneo. This is the first study to genetically characterize the full-length msp8 gene from clinical isolates of P. knowlesi from Malaysia; however, further functional characterization would be useful for future rational vaccine design.

• 수완나부미
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• 제16권1호
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• pp.175-203
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• 2024

With the enactment of international free trade and economic agreements and cooperation, BIMP-EAGA is an opportunity to accelerate development and economic growth in eastern Indonesia. This subregional cooperation could be used to reduce the development gap or inequality between the western and eastern regions, which are geographically, demographically, and economically different. This cooperation also may accelerate development in the border area. This study analyzes Indonesia's policies related to connectivity in BIMP-EAGA subregional cooperation and its implementation. The study results show that the National Secretariat of Sub-Regional Economic Cooperation has encouraged cooperation clusters ranging from natural resources, transportation, infrastructure, ICT, and tourism to MSMEs. In terms of connectivity, Indonesia is also involved in the development program of three economic corridors, namely West Borneo, East Borneo, and Sulu-Sulawesi. Indonesia's involvement in the three corridors has boosted the connectivity of Indonesia's territory, especially border areas, with neighboring countries. Connectivity has covered not only physical but also institutional and people-to-people dimensions. However, there are still several challenges, ranging from the standardization of the Customs, Immigration, and Quarantine (CIQ) mechanisms, improvement of institutional quality, and consistent strong political will among the involved parties. In addition, considering the vulnerability of the BIMP subregion to transnational crimes and acts of radical terrorism, BIMP-EAGA needs to think about solutions to overcome these cross-border security problems so that the momentum of development in the subregion may be sustained.

• Journal of Forest and Environmental Science
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• 제30권2호
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• pp.233-242
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• 2014

Estimating above-ground biomass is important in establishing an applicable methodology of Measurement, Reporting and Verification (MRV) System for Reducing Emissions from Deforestation and Forest Degradation-Plus (REDD+). We developed an estimation model of diameter at breast height (DBH) from IKONOS-2 image that led to above-ground biomass estimation (AGB). The IKONOS image was preprocessed with dark object subtraction and topographic effect correction prior to watershed segmentation for tree crown delineation. Compared to the field observation, the overall segmentation accuracy was 64%. Crown detection percent had a strong negative correlation to tree density. In addition, satellite-based crown area had the highest correlation with the field measured DBH. We then developed the DBH allometric model that explained 74% of the data variance. In average, the estimated DBH was very similar to the measured DBH as well as for AGB. Overall, this method can potentially be applied to estimate AGB over a relatively large and remote tropical forest in Northern Borneo.

• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.1925-1931
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• 2016

Background: The XRCC1 protein facilitates various DNA repair pathways; single-nucleotide polymorphisms (SNPs) in this gene are associated with a risk of gastrointestinal cancer (GIC) with inconsistent results, but no data have been previously reported for the Sabah, North Borneo, population. We accordingly investigated the XRCC1 Arg194Trp and Arg399Gln SNPs in terms of GIC risk in Sabah. Materials and Methods: We performed genotyping for both SNPs for 250 GIC patients and 572 healthy volunteers using a polymerase chain reaction-restriction fragment length polymorphism approach. We validated heterozygosity and homozygosity for both SNPs using direct sequencing. Results: The presence of a variant 194Trp allele in the Arg194Trp SNP was significantly associated with a higher risk of GIC, especially with gastric and colorectal cancers. We additionally found that the variant 399Gln allele in Arg399Gln SNP was associated with a greater risk of developing gastric cancer. Our combined analysis revealed that inheritance of variant alleles in both SNPs increased the GIC risk in Sabah population. Based on our etiological analysis, we found that subjects ${\geq}50years$ and males who carrying the variant 194Trp allele, and Bajau subjects carrying the 399Gln allele had a significantly increased risk of GIC. Conclusions: Our findings suggest that inheritance of variant alleles in XRCC1 Arg194Trp and Arg399Gln SNPs may act as biomarkers for the early detection of GIC, especially for gastric and colorectal cancers in the Sabah population.

• Fisheries and Aquatic Sciences
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• 제25권4호
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• pp.202-213
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• 2022

Antibiotic resistant Escherichia coli cases are increasing high especially in Southeast Asia. Illegal use of the antibiotic in the aquaculture farming may become the culprit of the outbreak and spread into environmental source. A study was conducted to: 1) detect the chloramphenicol (CAL)-resistant gene in E. coli isolated from three aquaculture farms and six rivers of northwestern Borneo and 2) investigate the correlation between cat gene with five common antibiotics used. Isolation of E. coli was done on Eosin methylene blue agar and characterized using indole, methyl red, Voges-Proskauer, citrate tests. E. coli isolates were subsequently tested for their susceptibility to five antibiotics commonly used in aqua-farming. The CAL-resistant E. coli were further analyzed for the presence of resistant genes (cat I, cat II, cat III, cat IV) using multiplex polymerase chain reaction. 42 bacterial colonies were isolated from a total of 80 individual water samples, 34 of which were identified as E. coli. Result showed 85.3% of the E. coli isolates were resistant to amoxicillin, 35.3% were resistant to tetracycline, 29.4% were resistant to CAL, 17.6% were resistant to nitrofurantoin and 8.8% were resistant to nalidixic acid. All of the 10 CAL resistant E. coli isolateswere detected with cat II genes; five isolates detected with cat IV genes; three isolates detected with cat III genes; and another two detected with cat I genes. Pearson correlation coefficient shows highly significant relationship between resistance pattern of CAL with amoxicillin; and CAL with tetracycline. Our findings provide the supplementary information of the CAL resistance gene distribution, thereby improving our understanding of the potential risk of antibiotic resistance underlying within this microbial ecosystem.

• 한국가구학회지
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• 제19권1호
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• pp.1-10
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• 2008

The shift to more practical and simpler lifestyle in the modern society is well reflected in people's preference when it comes to living room space and sofa designs. These elements are intentionally crafted to satisfy the customers' personal needs and wants, sense of comfort, aesthetic preference, and function. This study includes an analysis on the development and conceptualization of sofa designs, exterior materials, and colors of the furniture released by Hanssem, Livart, Borneo, Emons, and Jangin Furniture during the first half of 2007. Modern and practical sofas should be essentially designed according to the customers' demand and current trend. Such design principle is necessary to achieve and maintain competitiveness in the globalizing society.

• 한국가구학회지
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• 제21권5호
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• pp.367-379
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• 2010

Reflecting a particular place and time, from the agrarian society, through the Industrial Age, furniture has been one of the closest factors to our housing life and developed continuously. The information age representing digital, information and telecommunication generally gave a wide range of individuals and societies various phases of culture shock that had never been experienced so far, among which the change of the attitude toward work and marriage influenced on housing life style, they have required unexplored style and function of furniture additionally. People so called the singles has recently become a culture code reflecting the present time, they already have built their own territories in society, economy and culture. The ideas about a variety of furniture for the singles, which consider the housing culture of the singles, has been floated, therefore furniture for the singles are main streamed into home furniture category, required characteristics of furniture for the singles are drawn, furthermore some design examples are suggested on the basis of those characteristics in this study.

• 한국가구학회지
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• 제21권5호
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• pp.432-438
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• 2010

The study aimed at investigating the terms for each type of the current household furniture and at analyzing a standard based on those terms researched for the furniture, not including kitchen and child furniture. The study was conducted in sales and R&D division of major furniture brands such as Jang-In Gagu, Borneo, and Livart by surveying their staffs, and then, the investigated names were classified according to similar criteria and it was finally analyzed by the definite standard of the names made. In the results, the following 5 standards largely play roles for the naming. First, name according to requirements, second, construction of door, third, materials, fourth, shapes, and lastly, function. Except for the terms of "Sobari" expressed only as Japanese and "living room cabinet of Extension-type" using Korean and English along, most of the names show furniture in detail, and the names from Hangeul, Korean alphabet and from the English have recently and drastically increased with the lifestyle change influenced from Western world.

• 한국가구학회지
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• 제27권1호
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• pp.35-42
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• 2016

With radical increase in one-person households, the consumption market in Korea has also rapidly changed. In line with this movement, customized furniture for sing lecustomershas been recently introduced. Since sleeping more than eight hours a day is needed to maintain a healthy lifestyle, a suitable bed for a comfortable sleep is an essential product for single consumers. In order to respond to this trend, a study was conducted to examine the surface materials of a bed designed for singles. A complete enumeration survey was implemented for beds manufactured by ACEBED Co., Ltd., Simmons-k, Co, Ltd,. Keumsung Bed, Hanssem Co,. Ltd., Hyundai Livart Co,. Ltd,. Emons Furniture Co,. Ltd,. and Borneo International Furniture Co., Ltd. This study helped in the proper selection of surface materials that may be used in the development of furniture for single consumers.