• Title/Summary/Keyword: Bombus

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Expression and Antibacterial Activity of a Bombus ignitus Apidaecin in Baculovirus-Infected Insect Cells

  • Lee, Kwang-Sik;Je, Yeon-Ho;Jin, Byung-Rae;Sohn, Hung-Dae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.24 no.1
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    • pp.37-40
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    • 2012
  • The apidaecins are highly active against Gram-negative bacteria. Here, we show the expression and antibacterial activity of the bumblebee, Bombus ignitus, apidaecin. We PCR-amplified 51 bp of the active domain sequence of the B. ignitus apidaecin gene and expressed the recombinant B. ignitus apidaecin active domain in baculovirus-infected insect cells. The recombinant B. ignitus apidaecin active domain shows bactericidal activity against Gram-negative bacteria, including Pseudomonas tolaasii, a serious pathogen in cultivated mushrooms, but not Gram-positive bacteria. This result suggests that the active domain of the B. ignitus apidaecin is a potential antibacterial agent for the control of bacterial brown blotch diseases.

서양뒤영벌(Bombus terrestris)과 서양종꿀벌(Apis mellifera)의 시설참외(Cucumis melo var makuwa) 화분매개 활동 비교

  • 이상범;배태웅;윤형주;김삼은;심하식;김영수;이기열
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.04a
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    • pp.63-63
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    • 2003
  • 서양뒤영벌(Bombus terrestis)과 꿀벌(Apis melltfera)의 시설참외에서 화분 매개특성을 조사한 결과, 봉종별 방화활동에서 서양뒤영벌 일벌은 10시, 서양뒤영벌 수벌과 꿀벌은 12시에 방화활동 정점에 도달하였다. 암꽃 선호시간은 서양뒤영벌 일벌, 꿀벌 그리고 서양뒤영벌 수벌은 각각 10시, 12시 그리고 14-16시에 가장 높았다. 봉군 투입 후 서양뒤영벌 일벌과 꿀벌은 각각 2일과 5일째부터 정상적인 방화활동을 시작하여 24일간 정상적인 활동을 하였으나 서양뒤영벌 수벌은 3차례의 심한 기복이 있는 불안정한 방화활동을 보였다. (중략)

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Artificial hibernation for year-round rearing of the bumblebee, Bombus ignitus

  • Yoon, Hyung-Joo;Kim, Sam-Eun;Lee, Sang-Beom;Sim, Ha-Sik;Park, In-Gyun
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.04a
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    • pp.65-65
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    • 2003
  • Artificial hibernation is essential for year-round rearing of the bumblebee, Bombus ignitus that undergoes one generation per year It is known that keeping the queens in low temperature for two or three months is effective to terminate their diapause and develop the colony. Temperature, time and surroundings to keep the queens during artificial hibernation were investigated. (omitted)

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