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Expression and Antibacterial Activity of a Bombus ignitus Apidaecin in Baculovirus-Infected Insect Cells

  • Lee, Kwang-Sik (College of Natural Resources and Life Science, Dong-A University) ;
  • Je, Yeon-Ho (Department of Agricultural Biotechnology, College of Agriculture & Life Sciences) ;
  • Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University) ;
  • Sohn, Hung-Dae (College of Natural Resources and Life Science, Dong-A University)
  • Received : 2012.01.26
  • Accepted : 2012.02.25
  • Published : 2012.03.31

Abstract

The apidaecins are highly active against Gram-negative bacteria. Here, we show the expression and antibacterial activity of the bumblebee, Bombus ignitus, apidaecin. We PCR-amplified 51 bp of the active domain sequence of the B. ignitus apidaecin gene and expressed the recombinant B. ignitus apidaecin active domain in baculovirus-infected insect cells. The recombinant B. ignitus apidaecin active domain shows bactericidal activity against Gram-negative bacteria, including Pseudomonas tolaasii, a serious pathogen in cultivated mushrooms, but not Gram-positive bacteria. This result suggests that the active domain of the B. ignitus apidaecin is a potential antibacterial agent for the control of bacterial brown blotch diseases.

Keywords

References

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