• Title/Summary/Keyword: BolA

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BolA Affects Cell Growth, and Binds to the Promoters of Penicillin-Binding Proteins 5 and 6 and Regulates Their Expression

  • Guinote, Ines Batista;Matos, Rute Goncalves;Freire, Patrick;Arraiano, Cecilia Maria
    • Journal of Microbiology and Biotechnology
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    • v.21 no.3
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    • pp.243-251
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    • 2011
  • The gene bolA was discovered in the 80's, but unraveling its function in the cell has proven to be a complex task. The BolA protein has pleiotropic effects over cell physiology, altering growth and morphology, inducing biofilm formation, and regulating the balance of several membrane proteins. Recently, BolA was shown to be a transcription factor by repressing the expression of the mreB gene. The present report shows that BolA is a transcriptional regulator of the dacA and dacC genes, thus regulating both DD-carboxypeptidases PBP5 and PBP6 and thereby demonstrating the versatility of BolA as a cellular regulator. In this work, we also demonstrate that reduction of cell growth and survival can be connected to the overexpression of the bolA gene in different E. coli backgrounds, particularly in the exponential growth phase. The most interesting finding is that overproduction of BolA affects bacterial growth differently depending on whether the cells were inoculated directly from a plate culture or from an overnight batch culture. This strengthens the idea that BolA can be engaged in the coordination of genes that adapt the cell physiology in order to enhance cell adaptation and survival under stress conditions.

ON THE IDENTITIES OF BOL-MOUFANG TYPE

  • Solarin, A.R.T.
    • Kyungpook Mathematical Journal
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    • v.28 no.1
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    • pp.51-62
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    • 1988
  • In this work we study properties of five identities of Bol-Moufang type (1). We establish a necessary and sufficient condition for a loop to be an RC-loop. A corollary to this is the condition for a Bol loop to be an RC-loop. Some proerties of a generalization of RC-loops are discussed. We also state the relationship between the supernucleus M(G) of a loop G and the nucleii of loops which do not have the inverse property. Finally, we give two tables of non-associative RC-loops of order 16, which are not Bol loops.

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Characterization of the BolA Homolog IbaG: A New Gene Involved in Acid Resistance

  • Guinote, Ines Batista;Moreira, Ricardo Neves;Freire, Patrick;Arraiano, Cecilia Maria
    • Journal of Microbiology and Biotechnology
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    • v.22 no.4
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    • pp.484-493
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    • 2012
  • BolA protein homologs are widely distributed in nature. In this report, we have studied for the first time YrbA, the only BolA homolog present in Escherichia coli, which we have renamed ibaG. We have constructed single and multiple ibaG mutants, and overexpressed ibaG in wild-type strains, in order to characterize this gene. The ibaG phenotypes are different from the bolA-associated round morphologies or growth profiles. Interestingly, ibaG and bolA single-and double-deletion mutants grow faster and have higher viabilities in rich media, whereas the overexpressed strains are significantly growth impaired. However, the mutant strains have lower viabilities than the wild type in the late stationary phase, indicating that both bolA and ibaG are important for survival in difficult growth conditions. bolA, as a transcription factor, binds to some promoters, but ibaG does not interact with the same DNA regions. We have determined that ibaG is transcribed in an operon with the murA gene, involved in the synthesis of peptidoglycan precursors. ibaG was also seen to change its mRNA expression pattern in response to acidic stress. ibaG may thus represent a new gene involved in cell resistance against acid stress.

Comparisons of Interfacial Reaction Characteristics on Flip Chip Package with Cu Column BOL Enhanced Process (fcCuBE®) and Bond on Capture Pad (BOC) under Electrical Current Stressing

  • Kim, Jae Myeong;Ahn, Billy;Ouyang, Eric;Park, Susan;Lee, Yong Taek;Kim, Gwang
    • Journal of the Microelectronics and Packaging Society
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    • v.20 no.4
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    • pp.53-58
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    • 2013
  • An innovative packaging solution, Flip Chip with Copper (Cu) Column bond on lead (BOL) Enhanced Process (fcCuBE$^{(R)}$) delivers a cost effective, high performance packaging solution over typical bond on capture pad (BOC) technology. These advantages include improved routing efficiency on the substrate top layer thus allowing conversion functionality; furthermore, package cost is lowered by means of reduced substrate layer count and removal of solder on pad (SOP). On the other hand, as electronic packaging technology develops to meet the miniaturization trend from consumer demand, reliability testing will become an important issue in advanced technology area. In particular, electromigration (EM) of flip chip bumps is an increasing reliability concern in the manufacturing of integrated circuit (IC) components and electronic systems. This paper presents the results on EM characteristics on BOL and BOC structures under electrical current stressing in order to investigate the comparison between two different typed structures. EM data was collected for over 7000 hours under accelerated conditions (temperatures: $125^{\circ}C$, $135^{\circ}C$, and $150^{\circ}C$ and stress current: 300 mA, 400 mA, and 500 mA). All samples have been tested without any failures, however, we attempted to find morphologies induced by EM effects through cross-sectional analysis and investigated the interfacial reaction characteristics between BOL and BOC structures under current stressing. EM damage was observed at the solder joint of BOC structure but the BOL structure did not show any damage from the effects of EM. The EM data indicates that the fcCuBE$^{(R)}$ BOL Cu column bump provides a significantly better EM reliability.

Outflow properties of DIGIT embedded sources

  • Kang, Seonmi;Lee, Jeong-Eun;Choi, Minho;Evans II, Neal J.;Dunham, Michael M.
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.1
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    • pp.80.1-80.1
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    • 2015
  • We present a study of outflows on 24 embedded young stellar objects selected from the source list of the Dust, Ice, and Gas in Time (DIGIT) Herschel key program. To study the relation between the CO outflows observed in low-J transitions and the properties of protostars more consistently with a homogeneous data set, we mapped the CO outflows of the selected targets in the J = 1-0 and J = 2-1 lines with two Korean telescopes (SRAO and TRAO). We compare CO outflow force ($F_{CO}$) with the bolometric luminosity, ($L_{bol}$) bolometric temperature, and the FIR molecular line luminosities of CO, $H_2O$, OH, and [O I] detected by the Herschel-PACS observations. We find that $F_{CO}$ of J = 1-0 is greater than that of 2-1 by a factor of ~ 2. The well known correlation between $F_{CO\;2-1}$ and $L_{bol}$ is not very evident in our sample as a whole, but they show a rather strong correlation when IRAM 04191+1522 is excluded. IRAM 04191+1522 has relatively high $F_{CO\;2-1}$ in spite of its low $L_{bol}$. This object is a well-known VeLLO, which is believed in the quiescent phase of the episodic mass accretion in the embedded stage. $L_{bol}$ traces a current accretion, but $F_{CO\;2-1}$ traces accretion happened long ago. Therefore, the low-$L_{bol}$ with the high-$F_{CO\;2-1}$ can be explained by the episodic accretion. $F_{CO\;2-1}$ shows little correlation with individual FIR line luminosities of CO, $H_2O$, OH, while [O I] and total FIR line luminosity seem to have correlations with $F_{CO\;2-1}$. This result is interpreted as the accretion energy deposits on species differently depending on shock properties, but the total FIR line luminosity sums the total accretion energy dispersed to different species.

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Relative Clauses in a Modern Diachronic Corpus of Singapore English

  • Lee, Kit Mun
    • Asia Pacific Journal of Corpus Research
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    • v.1 no.1
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    • pp.31-60
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    • 2020
  • This paper investigates changes in relativization in Singapore English broadsheet newspapers from 1993 to 2016. One of the first diachronic studies in Singapore English (SgE), it also explores corresponding data from the diachronic Siena-Bologna (SiBol) news corpus. As SgE is in the endonormative stabilization phase in Schneider's (2007) Dynamic Model of postcolonial Englishes, divergence from British English (BrE) is to be expected. In this study, the dataset is a new Singapore English Newspaper (SEN) corpus compiled from local news articles in 1993, 2005 and 2016, and the corpus tool employed is Sketch Engine. The results reveal changes in relativization practices in SEN over the given period, many of which occur in a similar pattern as those identified in SiBol, albeit at varying rates of change. Most significant of these include a sharp decline in the which relativizer in restrictive relative clauses with non-animate antecedents, complemented by a rise in that. The change has been so rapid that although which relative clauses were more common than that clauses in 1993, that has subsequently overtaken which for both the corpora. One shift in SEN that is different from SiBol is the increase in frequency of non-restrictive relative clauses in SgE. The likely motivators for the changes in the two varieties are identified as colloquialization, densification and prescriptivism. The effect each of these factors could have had on the varieties are discussed, as well as the implications that the findings have on our understanding of the evolutionary status of SgE as a postcolonial variety.

ON SOME RESULTS OF BUMP-CHOIE AND CHOIE-KIM

  • Hundley, Joseph
    • Bulletin of the Korean Mathematical Society
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    • v.50 no.2
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    • pp.559-581
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    • 2013
  • This paper is motivated by a 2001 paper of Choie and Kim and a 2006 paper of Bump and Choie. The paper of Choie and Kim extends an earlier result of Bol for elliptic modular forms to the setting of Siegel and Jacobi forms. The paper of Bump and Choie provides a representation theoretic interpretation of the phenomenon, and shows how a natural generalization of Choie and Kim's result on Siegel modular forms follows from a natural conjecture regarding ($g$, K)-modules. In this paper, it is shown that the conjecture of Bump and Choie follows from work of Boe. A second proof which is along the lines of the proof given by Bump and Choie in the genus 2 case is also included, as is a similar treatment of the result of Choie and Kim on Jacobi forms.

Advanced Silicon Solar Cell Structures for Space Applications

  • Lee, S.H.;Kim, D.S.
    • Solar Energy
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    • v.17 no.2
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    • pp.23-33
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    • 1997
  • This paper reviews the advanced solar cell structures used in space. These are the structures which incorporate the back surface field and reflectors with very shallow and lightly doped emitters. Their use in space has shown that the thinner cells are more resistive to radiation damage than the thicker ones. It has been found that the charged particles affect both the surface and bulk of the cells used in space. This causes degradation in the output power, which in effect, can be explained by the degrading diffusion length of the cells. The PERL cells showed higher BOL(beginning of life) efficiency and almost the same EOL(end of life) efficiency as structures with wrap-around contact configuration fabricated on 10 ${\Omega}cm$ resistivity substrates. This observation lead to a conclusion that, the space cells do not necessarily need to have very high BOL efficiency except in specific missions which require such.

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