• 대한의용생체공학회:의공학회지
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• 제39권6호
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• pp.250-255
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• 2018

Accurate blood typing is the crucial factor for safe and successful blood transfusion and plays a very important role in organ transplantation and genetic information of forensic medicine. Microfluidic devices have been developed to overcome the limitations of the conventional blood typing methods. In this study, we demonstrate a Lamb wave-based device for simple blood typing in a sample droplet and we propose new indices for quantitative and accurate blood typing. Using Lamb wave-induced acoustic streaming in the droplet, the blood sample and the reagent can be mixed rapidly and red blood cells start to form clumps, which is agglutination. Based on the recorded image and video, the intensity of transmitted light through the sample droplet is evaluated to determine the blood type. Effect of the concentration of suspended red blood cells was evaluated and we found that 10% concentration of suspended red blood cells was suitable to observe the difference between aggregated and non-aggregated samples. Finally, sample with blood type A could be determined using anti-A reagent in our Lamb wave-based device. Our device enables simple and accurate blood typing, which can be applied to resource-limited environments.

• 한국콘텐츠학회논문지
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• 제19권6호
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• pp.565-574
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• 2019

본 연구는 혈액은행 자동화 장비 QWALYS-2 up (Diagast, Loos Cedex, France)의 유용성을 평가하기 위하여 수행되었다. 2013년 10월 1일부터 10월 31말까지 1개월간 강원대학교병원 진단검사의학과에 의뢰된 ABO 및 RhD 혈액형 검사 1,636건, 항체선별검사 1,374건을 대상으로 하였고, ABO 및 RhD 혈액형 검사의 평가는 200건을 대상으로 하였다. 혈액은행 자동화장비의 경제성을 평가하기 위해서는 2018년 12월을 기준으로 장비 도입 전인 2013년 1월부터 장비 도입 후 5개년의 ABO 및 RhD 혈액형 검사와 항체선별검사의 단가를 비교하였다. 본 연구의 주요 결과는 ABO 및 Rh 혈액형 검사 결과, 슬라이드나 시험관을 이용한 수기법의 결과가 100% (200/200) 일치하였고 동종항체 선별검사 결과, 불일치 3검체는 수기법으로 재검을 한 결과, 음성을 보여 QWALYS-2 up에서 위양성의 결과를 나타냈으나, 반응의 정도를 비교한 결과 QWALYS-2 up에서 상대적으로 높게 나타났다. 결론적으로 혈액형자동화 장비 도입 전후의 효과를 평가한 결과, 기존의 수기법과 비교하였을 때 높은 일치율을 보였으며, 정확도 면에서도 신뢰할 만한 것으로 판단되었다. 도입 전후의 시약의 비용은 증가하였지만 인건비의 절감을 감안하면 경제적으로도 유용하다고 평가할 수 있다.

• 한국수정란이식학회지
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• 제3권1호
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• pp.31-37
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• 1988

This study was carried out to produce the antisera for the blood typing in cattle. Blood types of eighty cattle were previously determined by 56 kinds of internationally standardized antisera from Japan. The donorrecipient animal arrangements were determined according to tile previously determined blood types of animals by the computer program SS-l for efficient production of antisera. Six kinds of standard antisera, H,B', 12, C2, Z, U2, were produced by isoimmunirzation and absorption methods.

• 대한수혈학회지
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• 제29권3호
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• pp.310-319
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• 2018

배경: 최근 차세대염기서열분석법(Next Generation Sequencing: NGS)을 이용한 HLA 형별 분석에 대한 연구가 활발히 진행되고 있다. 이에 HLA 고해상도 분석법의 내재적 한계인 위상 모호성의 문제를 해결하고, 대량 검체 처리가 가능한 NGS 기반 고해상도 HLA 형별 검사법을, 자체 기술로 개발하고자 본 연구를 실시하였다. 방법: HLA NGS를 위한 핵산 추출 조건, 라이브러리 제작 및 PCR 체계 확립, 그리고 생물정보학을 이용한 HLA 형별 분석법을 개발하였다. 본 기관에서 개발한 NGS 기반 HLA 형별 검사의 정확성을 알아보기 위해 SSOP법으로 HLA 형별을 알고 있는 192개 검체와 SBT법으로 HLA 형별을 알고 있는 28개 검체에 대해 NGS 기반으로 검사한 HLA-A, -B 그리고 -DR 형별 결과를 비교해 보았다. 결과: 두 단계의 PCR을 통한 DNA 라이브러리 제작과 MiSeq (Illumina Inc., San Diego, USA) 기기를 이용한 NGS 시퀸싱 그리고 데이터 분석 시스템을 구축하였다. 기존에 HLA 형별을 알고 있는 220개 혈액 검체에 대해 NGS 기반 HLA 형별검사 결과가 모두 일치함을 확인하였다. 결론: NSG 기반 HLA 형별 검사법은 많은 검체를 효율적인 시간 내에 처리가 가능하여 조혈모세포기증 희망자 HLA 검사 등에 유용할 것으로 기대된다.

• 한국콘텐츠학회논문지
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• 제14권3호
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• pp.346-351
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• 2014

안전한 수혈을 위한 정확한 혈액형 검사는 필수적이다. 최근에는 혈액은행 검사 분야에 검사의 오류를 줄이고 검사의 효율을 높이기 위해 자동화 장비가 도입되어 사용되고 있으나 중소병원에서 이용하기에는 매우 고가로 도입하기가 어렵다. 이에 본 연구에서는 혈액형 검사결과를 저장 및 재확인 할 수 있고, 이미지 프로세싱에 의해 결과가 판독되는 원주응집법을 이용한 혈액형 검사 판독기를 개발하였다. ABO 및 RhD 혈액형 검사가 의뢰된 148개의 검체와 비예기항체 검사가 의뢰된 154개의 검체를 대상으로 판독 결과를 비교한 결과, 양성과 음성의 판독 및 반응강도의 판독이 100%일치하였다. 본 연구에서 개발된 판독기는 추가적인 검증을 거친다면 중소형 병원에서도 쉽게 도입하여 효율적이며, 경제적으로 이용할 수 있을 것이다.

• 한국수정란이식학회지
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• 제9권2호
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• pp.197-205
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• 1994

This experiment was carried out to clarify the pedigree identification from blood typing of 301 Hoisteins in National Animal Breeding Institute(N.A.B.I.). Twenty kinds of standard reagent standardized by Insternational Society for Animal Blood Group Research provied from KNC improvement center, N, L, C, F. were used as the reference reagents in this study. The highest frequency of antigenic facfors was obtained from X$_2$in blood typing of 301 Holsteins. The frequency of X$_2$ was 0.714.In A blood system, four kinds of phenogroups were observed. The gene frequencies of Al and Z' phenogroups were equally 0.027.This frequency was greatly lower than those of breeds of Southern European and Zebu cattle. In B blood Systern, nineteen kinds of blood type were appeared. The appearance frequency of Gx blood type was 0.259, whish was higher than the others. In C blood system, thirty kinds of blood type were observed. The appearance frequency of X$_2$ blood type was the highest(0.189). In F blood system, three kinds of alleles were detected. The gene frequency of F allele was higher than that of V(0.105). However, the frequency of F allele(0.327) was greatly lower than that of "- /- " allele. In S blood system, twelve kinds of blood type were appeared and showed sirnilar appearance frequencies except " - / - " allele. From the results of the pedigree identification from 8 sires and 28 progenies of them, the accuracy of pedigree identification was 92.9%.ification was 92.9%.

• 대한수의학회지
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• 제31권4호
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• pp.397-402
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• 1991

The present experiments were undertaken to produce the standard antiserum for equine blood typing. The following results were obtained through ISO and Hetero Immunizations of the horses whose blood typing was analysed in the Laboratory of Racing Chemistry of Japan. 1. Of the 21 combinations of ISO-immune, 17 horses were produced antibody (about 80%) 2. Antibody titers were increased from early 1 week to late 5 weeks and any antibody titers were not be obtained in spite of the using of adjuvant and 10 repeated injections in the other 4 horses. 3. High antibody titers were obtained within the earliest period in the Dd antigen but were not increased over 32 times in spite of 8~10 repeated injections in the antigen. 4. Antibody were easily produced in the Ca antigen of ISO-Immune but production of antisera were tailed due to abscence of absorbed blood cell. 5. Antibody titers of 1,024 times were obtained through 5 injections in the Ca of HeteroImmune 6. Of the produced 15 antisera (16 system), 13 antigen (5 system) were absorbed.

• 한국수정란이식학회지
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• 제10권1호
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• pp.23-31
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• 1995

Several kinds of analytic methods for genetic polymorphism in cattle, including bovine blood typing, PCR-RFLP, BoLA and microsatellite typing were described. A few respect to consider for choosing method for actual application of genetic polymorphism were emphasized. The probability of relationship between characteristics and gene concerned, repetibility and easiness of methods applied and the possibility of clarification for segregation pattern should be deliberated.

• 대한수의학회지
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• 제42권4호
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• pp.469-473
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• 2002

This study was carried out to investigate the blood groups(Aa, Ca, Dc, Qa, Ua) and antierythrocyte antibody associated with neonatal isoerythrolysis(NI) in Thoroughbred horses. The blood groups from 1,351(35 stallions, 1,316 mares) Thoroughbred horses tested by serological procedures, and antierythrocyte antibody from 52 mares by indirect antiglobulin test. The blood groups(factor) of Aa, Ca, Dc, Qa and Ua positive were 97.1%, 100%, 91.4%, 82.9%, and 17.1% in stallions, respectively, and were negative 3.5%, 6.2%, 25.1%, 18.3%, and 77.1% in mares, respectively. These mares are considered to be at risk for production of an NI foals. The antierythrocyte antibody was not detected by this technique in all mares. These results suggest that the all breeding mares without blood groups Aa(3.5%) and Qa(18.3%) should be selected a appropriate stallion to prevent of neonatal isretythrolysis during the breeding season in Thoroughbred horses.

• 한국동물위생학회지
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• 제41권4호
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• pp.271-276
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• 2018

This study was conducted to investigate the prevalence of dog erythrocyte antigen (DEA) 1 with DEA 1.1 and DEA 1.2 on 122 Jindo dogs (29 males, 93 females) from 2014 to 2015 using a monoclonal antibody card kit (blood typing card kit, Korea Animal Blood Bank Inc., South Korea). Among the tested dogs, 14.8% (18/122) were positive for the DEA 1.1 antigen and 85.2% (104/122) were positive for the DEA 1.2 antigen. The prevalence of positive types for the DEA 1.2 antigen was significantly higher than the DEA 1.1 antigen (P<0.01). The prevalence of positive types for the DEA 1.1 antigen was higher in white-haired Jindo dogs than yellow-haired dogs (P<0.05). However, there was no gender difference in the prevalence of the DEA 1.1 antigen (P=0.665). The incidence of sensitization after the first transfusion without blood group test was 12.6% and the incidence of acute hemolytic transfusion reaction after the second transfusion in the same immunized dogs was 1.6%. Therefore, the blood group test for the DEA 1 antigen should be performed for Jindo dogs to ensure safe and effective transfusion therapy and further studies remain to be conducted for other DEAs among Jindo dogs.