• 동의생리병리학회지
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• 제25권2호
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• pp.195-201
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• 2011

Nardostachys chinensis;Anti-proliferation;Cell cycle arrest;Differentiation;U937 cells; This study was conducted to determine the analgesic effect of Sokyungwhalhyul-tang(SKWHT) using the model of peripheral neuropathic pain model. A model of neuropathic pain was made by ligating left 5th lumbar spinal nerve of rats. After 1 days, the extract of SKWHT was orally administered daily. Rats were divided into four groups; (1) Control group(n=6), (2) Experimental group I(SKWHT-OA1, 100 mg/kg, n=6), (3) Experimental group II(SKWHT-OA2, 300 mg/kg, n=6), (4) Experimental group III(SKWHT-OA3, 500 mg/kg, n=6). After that, we examined the withdrawl response of neuropathic rats legs by von Frey filament and Hot plate at pre, $1^{th}$, $4^{th}$, $7^{th}$, $14^{th}$, $21^{th}$ days after the induction of neuropathic pain. And also we examined c-fos, GOT, GPT and histological study of Liver at 21th days. von Frey filament and Hot plate were increase in experimental group I, II, III than Con. especially group III was most significantly analgesic effect than the other groups at $14^{th}$, $21^{th}$ days. In c-fos protein expression on spinal cord, group III was most significantly reduction immunoreactivity at $21^{th}$ days and in blood serum GOT & GPT levels and histologic finding of Liver in all experimental groups were no significant difference with Con at $21^{th}$ days. According to the above results, SKWHT(500 mg/kg) may have a significant analgesic effect on the neuropathic pain.

• Biomolecules & Therapeutics
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• 제3권4호
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• pp.266-272
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• 1995

The analysis of membrane receptors for hormones and neurotransmitters has progressed considerably by pharmacological and biochemical means and more recently through the use of specific antibodies. To generate and characterize a moloclonal antibody against $\beta$-adrenergic receptor, a synthetic $\beta$2-adrenergic receptor peptide (Phe-Gly-Asn-Phe-Trp-Cys-Phe-Trp-Thr-Ser-lle-Asp-Val-Leu) which may comprise part of $\beta$-adrenergic receptor ligand binding pocket was coupled to Keyhole Limpet Hemocyanin (KLH) and used as an immunogen. Male BALB/C mice were immunized with this antigen and the immunized spleen was fused with myeloma SP2/0-Ag14 cells to produce monoclonal antibodies. Two clones were obtained but one of monoclonal antibodies, mAb5G09, was used throughout in this study because the other clone, mAb5All showed weak immunoreactivity against KLH as well. The mouse monoclonal antibody mAb5G09 produced in this study showed immunoreactivity to peptide-KLH conjugates and also to human A43l cells and guinea pig lung $\beta$2-adrenergic receptor as revealed by ELISA and western blot. In the course of determination of the effects of mAb5G09 on $\beta$-receptor ligand binding, it was observed that mAb5G09 specifically bound $\beta$-adrenergic radioligand [$^3$H]dihydroalprenolol (DHA) with a dissociation constant (Kd) of 60 nM. The [$^3$H]DHA binding activity of mAb5G09 had characteristics of immunoglobulins and the binding activity was not observed in the control anti-KLH monoclonal antibody. The monoclonal antibody, mAb5G09 produced in this study may provide useful models for the study of the structure of receptor binding sites.

• Parasites, Hosts and Diseases
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• 제25권2호
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• pp.141-148
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• 1987

실험적으로 폐홉춘을 감염시킨 백서의 혈청내 항원을 ELISA 법으로 측정하였다. 시험용 혈청은 폐흡충의 피낭유충을 마리당 25개씩 먹인 총 22마리의 백서에서 감염후 12주가 될 때까지 채혈하여 얻었다. 폐흡충에 대한 특이항체는 폐흡충 감염 고양이의 혈청으로부터 ammonium sulfate 침전과 anionezchange chromatography를 이용, IgG를 분리한 후 affinity chromatography를 이용하여 순수분리하였다. 항원을 찾기 위한 ELISA는 소위 "double antibody sandwich method"를 사용하였다. 대조로서 사용한 감염전 혈청의 O.D.치 평균값은 0.04(표준편차 0.04)이었으며 감염후 O.D.치 (표준편차)의 변화를 보면 0.5주(3일)는 0.03(0.01), 1주는 0.55(0.50), 1.5주는 0.69(0.45), 2주는 0.20(0.19), 2.5주는 0.13(0.10)이었고 감염 3주 이후는 대조 혈청의 값으로 떨어졌다. 대조혈청의 $평균간+3{\times}$ 표준편차, 즉 0.16이하를 이 system에서의 비특이적 수치로 잡았을 때 이 이상의 수치를 나타낸 경우는 감염 후 1주부터 2.5주까지의 혈청에서만 관찰되었다. 한편 감염 12주 후에 총 10마리의 백서를 희생시켜 유충을 회수하여 보았다. 폐와 흉강에서는 평균 2.2마리, 근육에서는 평균 6.2마리의 유충을 회수할 수 있었다. 폐흡충 감염 백서에 있어 충체가 생산하는 항원은 감염 1주 후부터 혈청 내에서 검출되었다. 그러나 감염 3주후부터는 검출되지 않았는데 이것은 항원-항체 복합체 형성에 의한 반응 저해 현상에 의한 것으로 생각되었다.

• Korean Journal of Acupuncture
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• 제37권1호
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• pp.14-23
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• 2020

Objectives : We investigated the efficacy and safety of miniscalpel acupuncture (MA) treatment combined with non-steroidal anti-inflammatory drugs (NSAIDs) for chronic neck pain (CNP) in an assessor-blinded randomized controlled pilot trial to establish a basis for larger-scale randomized controlled studies on this subject. Methods : Participants (n=36) were recruited and randomly allocated to the MA group, NSAIDs and combined treatment group. The MA group received MA three times over three weeks. The NSAIDs group was administered orally with zaltoprofen 80mg t.i.d. over three weeks. The combined treatment group received MA and zaltoprofen in the same manner as MA and NSAIDs groups. The primary outcome was pain as assessed by a visual analogue scale (VAS) and the secondary outcomes were assessed using the Neck Disability index (NDI), EuroQol 5-dimension questionnaire (EQ-5D), and Patients' Global Impression of Change scale (PGIC). Assessments were made at week 0 (baseline), 1, 2, 3 (primary end point) during treatment and at week 7 (4 weeks after the end of treatment). Results : 35 participants completed the study. No serious adverse event occurred and blood test results were within normal limits. The improvement of VAS and NDI was significantly greater in combined and MA group than that in NSAIDs group (p<0.017). The combined group showed better outcomes in EQ-5D at visit 2 and 5, in PGIC at visit 4 than the NSAIDs group (p<0.017). No significant differences were found between combined and MA group. Conclusions : Our results suggest that both combined and MA group can be more effective in improving pain control than NSAIDs group. A large-scale clinical study is warranted to further clarify these findings.

• Journal of Hospice and Palliative Care
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• 제7권1호
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• pp.49-63
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• 2004

배경: 호스피스 암 환자에게 적합한 경과기록지의 부재 또한 적절한 암성 통증 관리의 장애요인으로서 기존의 "문제 지향식 의무기록"의 경과기록지 형식에서 벗어나 호스피스 암 환자를 위한 의무기록지(HOMR)를 개발하게 되었다. 방법 및 결과: 2004년 3월부터 5월 사이 고대 구로병원 호스피스 팀은 20여 차례의 모임을 갖고 호스피스 환자의 초기 평가 이후 경과기록에 사용할 수 있는 의무기록지를 개발하였다. 구성항목의 결정은 pilot study를 통해 수정과정을 거쳤다. HOMR은 A4용지 크기로 작성자를 위한 지침서 1장과 2장의 경과기록지로 구성되었다. 앞면에는 환자의 인적 사항, 현재 문제목록 및 활동 수행 능력 상태, 검사 결과, 1주일 간의 활력 징후 및 I/O, 배변 횟수가, 뒷면에는 통증 부위, 통증의 성격 및 강도, 약물 및 비약물 요법, 진정 정도, 동반 증상, 약물 부작용 등을 한꺼번에 기록할 수 있도록 구성되어 있다. 결론: 호스피스 암 환자를 위한 의무기록지는 짧은 여명과 다양한 신체 증상을 갖고 있으며 증상의 변화가 빠르지만 만성적인 경과를 보이는 호스피스 환자에게 적합하며 그 자체만으로도 교육자료로서의 가치가 높고 환자를 돌보는 데 있어 진료의 질을 평가하거나 심사할 수 있으므로 진료의 질을 높이는 역할도 기대된다.

• 한국식품과학회지
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• 제32권5호
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• pp.1206-1212
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• 2000

가미육미지황탕이 BB rat에서 유발되는 당뇨병을 개선시킬 수 있는가를 알아보았다. BB rat은 태아시기부터 생후 120일까지 가미육미지황탕을 공급받았으며 생후 21일된 BB rat 60마리(수놈 30마리, 암놈 30마리)를 무처치군인 BBDP군과 가미육미지황탕을 투여한 군인 BBDP-GY군으로 임의로 배치하여 생후 120일까지 사육하였다. BBDP-GY군의 쥐들에게는 가미육미지황탕을 16mL/㎏ B.W./day 분량으로 물에 섞어 주었다. 가미육미지황탕의 항당뇨 효과는 다뇨, 다음, 고혈당 및 체중감소와 같은 임상적인 요소들에 의하여 조사되었다. 그 결과 가미육미지황탕은 당뇨병의 발병시기를 지연시켰을 뿐만 아니라 당뇨병의 발병율도 현저하게 감소시켰다. 그러나 일단 발병이 되고 나서는 당뇨병의 진전을 지연시키거나 치유하는 효과가 없는 것으로 나타났다. 당뇨의 발병과 동시에 체중의 현저한 감소 및 물 소모량, 배뇨량 그리고 혈당에 있어 현저한 증가가 나타났고 당뇨의 발병이 있기 전에는 체중에 있어서 발병 2-4일전에 체중의 증가가 거의 없거나 또는 약간 있을 뿐 다른 항목들에서는 발병을 알리는 어떤 변화도 나타나지 않았다.

• 대한지역사회영양학회:학술대회논문집
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• 대한지역사회영양학회 2003년도 추계학술대회
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• pp.1048-1048
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• 2003

The purpose of this study was to investigate dietary behaviors, body image, hematological index and nutrient intake of female athletes in Incheon. The subjects were 112 female athletes (field and track: n=32, firing: n=27, fencing: n=29, swimming: n=14, badminton: n=10) from middle and high schools in Incheon. This cross-sectional study was conducted by a self-administered questionnaire. Fasting blood samples were obtained and analyzed for hemoglobin (Hb), hematocrit(Hct), ferritin, serum iron, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), unsaturated iron binding capacity (UBC), total iron binding capacity (TIBC) and transferrin saturation (TS). Nutrient intakes collected from 3 day-recalls were analyzed by the Computer Aided Nutritional Analysis Program. Statistical analysis was conducted using SPSS 10.0 program. The results are summarized as follows: Average age of field and track athletes, firing, fencer and swimmer was 14 years and that of badmintoner was 17 years. Most of female athletes had dietary problems such as unbalanced meals, skipping meals, and preference of processed foods. More than 60% of female athletes skipped breakfast. As for perception of body image, most of female athletes perceived themselves fatter compared to normal body image. Especially, field and track athletes were more significantly experienced weight control compared to the other athletes (p<0.05). As for physical burden during exercise, 56.3% of swimmer and 31.3% of field and track athletes answered “very hard”, which showed a significant difference (p<0.001) More than 80% of female athletes had experienced a vertigo during exercise (p<0.01). Also, more than 50% of female athletes except badmintoner had experienced an irregular menstruation (p<0.05). Average serum iron level (p<0.05) and TS (p<0.05) of field and track athletes were significantly lower compared to the other athletes. Serum ferritin of badmintoner, field and track athletes and fencer was significantly lower compared to firing and swimmer (p<0.05). Nutrient intakes of female athletes except vitamin B6, niacin and phosphorus were lower than the Korean RDA. Especially, calcium and iron intakes of female athletes were under the 50% of the Korean RDA. Therefore, proper nutrition education and supplementation are required for female athletes to encourage desirable food habits as well as to improve their nutritional status and exercise performance.

• 한국산학기술학회논문지
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• 제15권5호
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• pp.2914-2922
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• 2014

진행성위암의 위험요인을 파악하고 조기검진의 효과를 평가하고자 2007년 6월부터 2011년 12월까지 대전 소재 1개 대학교병원에서 위암으로 최초 진단과 수술을 받은 조기위암 171명과 진행성위암 환자 342명, 총 580명을 대상으로 설문조사와 의무기록 조사를 실시하였다. 두 군간에 카이제곱 검정과 로지스틱 회귀분석으로 교차비를 계산한 결과 진행성위암은 조기위암에 비하여 70세 이상에서(OR 2.393; 95%CI 1.329-4.310), 과거 흡연자(OR 1.612; 95%CI 0.970-2.680), 혈액형 A형(OR 1.784; 95%CI 1.035-3.075) 및 H. pylori균 감염(OR 1.699; 95%CI 0.905-3.191)에서 상대적으로 높았다. 또한 체중감소(OR 2.752; 95%CI 1.333-5.684)와 소화불량(OR 1.574; 95%CI 1.069-2.319)이 중요한 증상과 징후였다. 조기진단의 효과는 파악할 수 없었지만 조기진단이 성공적인 치료의 기회를 높이고 생존율을 향상시킨다는 것을 감안할 때 집단을 대상으로 조기검진을 고취시키는 교육과 선별검사가 요구된다.

• Journal of Nutrition and Health
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• 제45권5호
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• pp.452-461
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• 2012

There is increasing evidence that dietary factors in plant-based diets are important for the prevention of chronic disease. Especially, phytonutrients in fruits and vegetables have been recognized as major contributors for the decreased level of oxidative stress. In this study, the effect of switching the dietary habit to high consumption of fruits and vegetables were evaluated on the parameters of serum antioxidant status in healthy high school students. Forty one students participated in a randomized controlled trial and were assigned to the control group (n = 18) or the intervention group (n = 23). The intervention group was provided for 8 weeks with the main food source being whole grain and vegetables. Anthropometric measurements, blood parameters and dietary intakes were measured, and compared before and after study. After 8 weeks, weight and BMI were significantly decreased in the intervention group (p = 0.000). The serum diacron reactive oxygen metabolites (d-ROMs) test resulted in a significantly decreased level only in the intervention group (p < 0.05) after 8 weeks, but serum biological antioxidant potential (BAP) was increased significantly in both groups (p < 0.001). Intake of energy, total fat, cholesterol and sodium in the intervention group were significantly decreased after 8 weeks (p < 0.05). Also, the intervention group had significantly increased vitamin and phytonutrient intakes of all-trans-${\beta}$-carotene, ${\alpha}$-carotene, ${\alpha}$-tocopherol, ascorbic acid, and total phenols (p < 0.05). Overall, the results of this study suggest that whole grain, fruits, and vegetables supplementation showed improvement of the adolescent health.

• Journal of Yeungnam Medical Science
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• 제12권1호
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• pp.96-104
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• 1995

여러가지 색전물질이 조직내에서 어떤 변화를 야기시키며 또한 시간경과에 따라 어떤 변화를 보이게 되는지를 알아보기 위해 EVAL, Histoacryl, Ivalon을 이용하여 토끼의 신동맥을 통해 색전을 실시하였으며 시간경과에 따라 조직학적으로 검사하여 다음과 같은 결과를 얻었다. EVAL은 1주일이내의 초기단계에는 혈관 내에서 별다른 조직변화를 일으키지 않았으나 2주일 이내의 중기단계에는 혈관벽의 비후를 나타냈다. Histoacryl은 1주일이내의 초기단계에 벌써 심한 섬유소양 변성을 보여 심한 조직변화를 일으킨다는 것을 알 수 있었고 실험 예의 반수에서 신동맥의 폐쇄에 의한 경색을 볼 수 있어 강한 조직유착성을 시사해 주었으며 실제 환자에 적용함에 있어 주의를 환기시켜 주었고 혈류가 빠르거나 누공등이 있는 경우에 더욱 적절히 사용될 수 있음을 알 수 있었다. Ivalon은 초기에는 혈관염의 소견을, 중기에는 이물질반응에 의한 거대세포를, 후기에는 심한 섬유화의 소견을 보여 주었고 수술을 대신하는 치료적인 목적보다는 혈류를 줄여 수술을 보다 용이하게 해주는 등 수술의 보조적인 이용에 바람직할 것으로 기대된다.