• 생약학회지
• /
• 제47권1호
• /
• pp.49-54
• /
• 2016

In our search for natural products affecting blood circulation, the n-butanol fraction from whole plant of Glehnia littoralis Fr. Schm. (GLB) improved blood lipid parameters, and ameliorated obesity in high-fat-diet (HFD)-fed C57BL/6 mouse model. Hyperlipidemia was induced by high-fat-diet for 4 weeks, and then GLB was orally administrated with 400 mg/kg/day for 4 weeks. GLB-treated group showed that the gain in body weight was significantly attenuated, the levels of total cholesterol and triglyceride significantly lowered on blood chemical analysis, and significantly prolonged the mice bleeding time when compared with those of HFD control group. Concomitantly, phytochemical composition of GLB was investigated by HPLC-hyphenated spectroscopy, and two major phenolic compounds, rutin and chlorogenic acid were identified in the GLB. Taken together, these results indicate that GLB has cardiovascular protective effects and could be a natural medicine candidate for the prevention of cardiovascular disease.

• 한국양식학회지
• /
• 제17권4호
• /
• pp.268-274
• /
• 2004

본 연구에서는 넙치, Paralichthys olivaceus를 이용하여 oxytetracycline (OTC)처리농도에 따른 체내에서 혈액 생리학적인 변화, 항산화 효소 및 열충격단백질(HSP)등의 변화를 조사하여 항생제의 안정적인 사용에 대한 기초 자료를 제공하고자 본 실험을 수행하였다. 실험구는 각각 0 (대조구), 100, 300 및 500ppm으로 설정하였으며, 1시간 약욕처리 후 순환시켜 0, 1 및 3시간 후에 혈액성상을 분석하였다. 혈액 중 hematocrit(Ht)수치는 전 실험구에서 감소하는 경향을 보였으나 유의한 차이는 나타나지 않았다. Red blood cell (RBC)는 농도 의존적으로 감소하는 경향을 나타내었다. 또한, 혈청 글루코오스 농도는 농도 의존적으로 증가되는 경향을 나타내었으나, 노출 이후 3시간 경과 후에는 이전 대조구 수준으로 감소되었다. 혈중 단백질량은 0시간째 전 실험구에서 감소되었다가 이후 증가하는 경향을 나타내었으나, 처리전의 대조구와 비교하여 유의하게 낮은 값을 나타내었다. SOD 효소 활성은 노출 직후 300 및 500ppm에서는 유의하게 높은 값을 나타내었다. CAT효소활성은 OTC처리 농도에 비례하여 감소하는 경향을 보였으나 유의한 차이는 나타내지 않았다. 그리고 HSP-70의 단백질량은 100 ppm및 500 ppm처리구에서 대조구에 비해 높게 발현되는 것이 확인되었다. 그러나 HSP-70 mRNA는 다소 증가하는 경향은 보였으나, 모든 실험구에서 유의한 차이는 나타나지 않았다.

• Journal of Pharmaceutical Investigation
• /
• 제29권3호
• /
• pp.235-240
• /
• 1999

Bioequivalence of two clarithromycin tablets, the $Klaricid^{TM}$ (Ciba-Geigy Korea Ltd., Seoul, Korea) and the LG clarithromycin (LG Chemical Co., Ltd., Seoul, Korea), was evaluated according to the Korean Guidelines for Bioequivalence Test (KGBT 1998). Sixteen normal male volunteers $(20{\sim}26\;years\;old)$ were randomly divided into two groups and a randomized $2{\times}2$ cross-over study was employed. After one tablet containing 250 mg of clarithromycin was orally administered, blood sample was taken at predetennined time intervals, and the concentrations of clarithromycin in serum were detennined using HPLC method with electrochemical detector. The pharmacokinetic parameters $(AUC_t,\;C_{max}\;and\; T_{max})$ were calculated and ANOVA was utilized for the statistical analysis of parameters. The results showed that the differences in $AUC_t$, $C_{max}$, and $T_{max}$ between two tablets based on $Klaricid^{TM}$ tablet were 4.06%,2.67% and -9.70%, respectively. The powers $(1-{\beta})$ for $AUC_t$, $C_{max}$ and $T_{max}$ were 83.53%, 92.34% and 96.64%, respectively. Detectable differences $({\Delta})$ and 90 % confidence intervals $(a=0.05) $were all less than ${\pm}20%$. All the parameters above met the criteria of KGBT 1998, indicating that LG clarithromycin tablet is bioequivalent to $Klaricid^{TM}$ tablet.

• 한국어병학회지
• /
• 제19권3호
• /
• pp.253-265
• /
• 2006

본 연구는 동물용 생화학 분석장치를 이용하여 분석한 어류 혈액검사 항목 가운데 적용할 수 있는 분석항목의 활용도를 평가함과 동시에 어류 혈액학적 건강진단에 필요한 분석항목의 참고자료를 얻고자 하였다. 경남 지역의 해상가두리 어류 양식장에서 사육중인 육안적으로 보아 건강한 조피볼락 (Sebastes schlegeli), 참돔 (Pagrus major), 돌돔 (Oplegnathus fasciatu) 및 감성돔 (Acanthopagrus schlegeli) 총 522마리를 대상으로 hematocrit 와 간편하게 혈액성분을 측정할 수 있는 FUJI DRI-CHEM 3000 기종을 이용하여 혈장화학적 성분 분석을 실시하였다. Hematocrit (Ht)는 microhematocrit법에 의해 그리고 hemoglobin (Hb), 총단백 (TP), 알부민 (ALB), 알칼리성포스파타제 (ALP), 요소질소 (BUN), 젖산탈수소효소 (LDH), 중성지방 (TG), 총콜레스테롤 (TCHO), 크레아티닌 (CRE), aspartate aminotransferase (AST), alanine aminotransferase (ALT), 포도당(GLU)은 정상범위의 표준자료가 이미 프로그램화되어 장치된 동물용 생화학 분석장치인 FUJI DRI-CHEM 3000 기종을 이용하여 분석하였다. 그 결과, 측정된 Hb와 혈장화학성분의 실제 값들은 본 기종의 하한 및 상한 한계치를 상당히 벗어났다. 본 장비로부터 분석값을 검출하지 못하였던 비율을 살펴보면, ALB 및 ALT는 68～66%, LDH, TP, ALP 및 GLU는 42～21%, TCHO, AST, TG, Hb 및 CRE는 18～3%의 범위를 나타내었으나, BUN의 분석값은 모두 검출한계를 벗어나지 않았다.

• Clinical and Experimental Pediatrics
• /
• 제52권1호
• /
• pp.28-35
• /
• 2009

목 적 : 액상 대두기반 영아식이 개발되어 시판되고 있으나 이에 대한 영양학적 연구는 미흡한 상태이다. 액상 대두영아식을 섭취하는 경우 영유아의 성장 및 발달을 모유 및 우유기반 영유아식을 먹이는 경우와 비교하였다. 방 법 : 2001년 6월부터 203년 8월까지 충북대학교병원에서 만기 정상분만으로 출생한 신생아를 대상으로 모유군(n=20)과 대두 영유아식(n=19) 및 우유 영유아식군(n=12)으로 나누어, 생후 첫 3개월간은 모유 및 인공 영유아식을 각 군에 해당하는 식이를 먹이고 생후 4개월부터는 이유식을 자유롭게 하도록 했다. 신체계측으로는 출생 시, 1, 2, 4, 5, 12개월, 그리고 36개월에 신장, 체중, 두위, 삼두박근 피부두께, 그리고 상완둘레를 측정하였다. 일반혈액 및 혈청의 화학검사는 생후 5, 12 그리고 36개월에 시행하였다. 결 과 : 생후 5개월까지는 대두 영유아식군의 체중이 가장 낮았으나 신장은 차이가 없었고, 이후 체중과 신장은 36개월까지 모든 영유아식군 간에 차이가 없었다. 혈중 헤모글로빈은 5개월과 12개월에 모유군이 가장 낮았다(P<0.05). 혈청화학 검사에서는 대두 영유아식군에서 생후 5개월에 BUN은 가장 높았고, cholesterol, 인, 포타슘은 가장 낮았으나, 그 이후는 모든 화학검사 수치의 차이가 없었다. 결 론 : 대두기반 영유아식을 준 경우에 생후 3년간 우유 영유아식군이나 모유군에 비하여 신체의 성장에는 차이가 없었다. 대두기반 영유아식이 우유기반 영유아식이나 모유에 비하여 무기질 함량이 높은데도 불구하고 생후 5개월에 혈청 인 및 포타슘이 낮아 무기물의 흡수율을 높이는 방법을 개발해야 할 것으로 보인다.

• Toxicological Research
• /
• 제16권4호
• /
• pp.302-309
• /
• 2000

The purpose of this study is to investigate toxic effects of iso-butylalcohol (iBA) in Sprague-Dawley (SD) rats under the exposure of 6 hours a day, 5 days a week for 13 weeks by inhalation, and to evaluate the occupational safety of iBA in comparison with the permissible exposure level (PEL) stipulated by the Occupational Safety and Health Administration (OSHA). iBA did not induce any abnormal changes from the aspects of clinical signs, feed consumption, ophthalmic test, urinalysis, hematology and blood chemistry during and at the terminal of the inhalation toxicity tests. We did not find any abnormal findings in the gross and microscopic observations due to the inhalation of iBA. There was no alteration in relative organ weights by the inhalation of iBA. No observed adverse effect level (NOAEL) of iBA was considered to be more than 3,000 ppm in rats under the inhalation of 6 hours a day, 5 days a week for 13 weeks. Fifty ppm of iBA, the PEL regulated by OSHA, is too conservative for working places. As iBA showed no abnormal observations in all the experimental parameters at any concentration under this experimental condition, we suggest that 150 ppm is safe enough for the PEL of iBA in the working areas, even taking into onsideration that OSHA lowered the PEL to 50 ppm for fear of the probable risk of its skin irritation.

• Preventive Nutrition and Food Science
• /
• 제11권2호
• /
• pp.115-119
• /
• 2006

The object of this study was to examine whether the germanium fortified yeast administered to SD rat is accumulated in the liver and kidney. The administration doses were within 2,000 mg/kg which is the level of NOAEL (no observed adverse effect level) proved through the previous study of single/consecutive oral toxicity test. There were no significant clinical symptoms and mortality following the administration of organic germanium-fortified yeast (0, 500, 1,000, 2,000 mg/kg) during the whole test period, and also no difference in the consumed amount of feed and water for each group. No significant abnormalities of hematology and blood chemistry parameters were found in all groups of organic germanium-fortified yeast (0, 500, 1,000, 2,000 mg/kg). The amount of germanium accumulated in liver and kidney was 0 g/kg by ICP-AES method in the group of organic germanium-fortified yeast. In the positive control group of $GeO_2$ (150 mg/kg), the amount of accumulation was shown to 3135.0 and 4277.2 g/kg in each female and male kidney and 1044.3 and 2135.8 g/kg in each female and male liver, respectively. Organic germanium-fortified yeast, a biosynthetic product resulting from putting germanium into yeast, did not show any clinical symptoms, blood chemical significance, and residues in kidney and liver. It could be inferred that the non-toxic amount of organic germanium-fortified yeast was up to 2,000 mg/kg.

• 한국환경보건학회지
• /
• 제14권1호
• /
• pp.33-38
• /
• 1988

Natural mineral water is generally quite different from ordinary drinking water due to its original nature and various properties. The complexity of natural mineral water requires, therefore, not only to identify its nature and proper characteristics, but also to classify them by a reasonable scientific basis of comparison. The study was concentrated on a possible classification technique to natural mineral waters by their constitutions and physico-ehemical properties. The classification was carried out by the computation of such numerical parameters as ionic equivalent percentage, electrolytic conductance or mobility, ionic molecular weight, molecular concentration, equivalent conductivity and degree of ionization in consideration of the determinative criteria as follows -particular single element or molecule -major components of natural waters as bicarbonate, sulphate, chloride,caloride, calcium, magnesium, and sodium -moleculat concentration related to blood osmotic pressure -water temperature at emergence from spring -contents of free carbon dioxide (CO2) -pH value of water -total dissolved solids or salts (NaCl) The results obtained proved out to be clearly distinguhhable from ordinary drinking water as far as concern natural mineral water as an example on the subject -simple water -bicarbonate-predominating water -cold spring -carbonated-non gaseous water -weak alkaline water -non saline water Putting these various results together, the sample turned out to be a kind of natural mineral water that can be used as a drinking water if microbiologically safe.

• Natural Product Sciences
• /
• 제10권4호
• /
• pp.168-172
• /
• 2004

In India, Gymnema sylvestre due to the unique property of the plant to antagonize the sweet taste is known as 'Gur-mar'. It has several ethnomedicinal values as various tribals/traditional communities and rural peoples of India find diverse medicinal uses viz. antidiabetic, stomachic, diuretic, and is useful in cough and throat troubles. Besides, it has strong effect on reducing blood sugar. The present communication deals with the detailed pharmacognostical evaluation of the aerial parts of G. sylvestre collected from three places of the country-Varanasi (U.P), Panchmarhi (M.P), Salem (Tami Nadu) and commercial sample procured from local market. The botanical and physico-chemical parameters of all the samples were quite similar though little variations were observed in foaming index, alcohol and water soluble extractives of local sample. The microscopic characteristics of the drug are horse shoe shaped petiole with 3 amphicribal vascular bundles, sieve tubes well developed; anomocytic stomata only on the abaxial surface of the leaf, the fan shaped amphicribal vascular bundle, presence of intraxylary phloem. The TLC fingerprint profile of all the samples was more or less similar only the quantity of some of the compounds varied.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제8권5호
• /
• pp.279-285
• /
• 2003

Lidocaine and galactose loading tests were performed on a bioartificial liver (BAL), an extracorporeal medical device incorporating living hepatocytes in a cartridge without a transport barrier across the membranes. The concentration changes were analyzed using pharmacokinetic equations to evaluate the efficacy and limitation of the proposed method. Lidocaine and galactose were found to be suitable drugs for a quantitative evaluation of the BAL functions, as they did not interact with the plasma proteins or blood vessels, making their concentrations easy to determine. The drug concentration changes after drug loading were easily analyzed using pharmacokinetic equations, and the BAL functions quantitatively expressed by pharmacokinetic parameters, such as the clearance (CL) and galactose elimination capacity (GEC). In addition, these two drugs have already been used in clinical tests to evaluate human liver functions over long periods, and lidocaine CL values and GEC values reported for a normal human liver. Thus, a comparison of the CL and GEC values for the BAL and a natural liver revealed what proportion of normal liver functions could be replaced by the BAL.