Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Method for Evaluating Metabolic Functions of Drugs in Bioartificial Liver

  • Park, Yueng-Guen (Laboratory of Tissue Engineering, Korea Cancer Center Hospital, KAERI, Institute for Frontier Medical Science, Kyoto University) ;
  • Hiroo Iwata (Institute for Frontier Medical Science, Kyoto University) ;
  • Seiji Satoh (Department of Gastroenterological Surgery, Kyoto University) ;
  • Takehiko Uesugi (Department of Gastroenterological Surgery, Kyoto University) ;
  • Ryu, Hwa-Won (Faculty of Applied Chemical Engineering Chonnam National University, Institute of Bioindustrial Technology, Chonnam National University)
  • Published : 2003.10.01
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Abstract

Lidocaine and galactose loading tests were performed on a bioartificial liver (BAL), an extracorporeal medical device incorporating living hepatocytes in a cartridge without a transport barrier across the membranes. The concentration changes were analyzed using pharmacokinetic equations to evaluate the efficacy and limitation of the proposed method. Lidocaine and galactose were found to be suitable drugs for a quantitative evaluation of the BAL functions, as they did not interact with the plasma proteins or blood vessels, making their concentrations easy to determine. The drug concentration changes after drug loading were easily analyzed using pharmacokinetic equations, and the BAL functions quantitatively expressed by pharmacokinetic parameters, such as the clearance (CL) and galactose elimination capacity (GEC). In addition, these two drugs have already been used in clinical tests to evaluate human liver functions over long periods, and lidocaine CL values and GEC values reported for a normal human liver. Thus, a comparison of the CL and GEC values for the BAL and a natural liver revealed what proportion of normal liver functions could be replaced by the BAL.

Keywords

References

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