• Title/Summary/Keyword: Blood cell count

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Hematological and Serum Chemical Values in Pere David′s Deer and Wapiti (사불상 및 와피티 사슴의 혈액 및 혈청화학치)

  • Shin Nam-Sik;Kwon Soo-Wahn;Han Duk-Hwan;Lee Heung-Shik
    • Journal of Veterinary Clinics
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    • v.11 no.1
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    • pp.471-478
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    • 1994
  • The hematologic values and serum chemistry were examined healthy male 12 Pere David's Deer and 8 Wapiti at the Farmland Zoological Garden. In wapiti, the blood values collected from horn and jugular vein were compared. The results were as follows: 1. There were not any significant differences in hematologic values and serum chemistry of blood col. looted from horn and jugular vein in wapiti. 2. In Pere David's Deer, Mean$\pm$SD Values of White Blood Cell(WBC) count were 4673.50$\pm$1893.42/${mu}ell$, of Neutrophils 65.42$\pm$12.7%, of Lymphocytes 31.50$\pm$8.68%, of Monocytes 0.25$\pm$0.43%, of Eosinophils 0.17$\pm$0.37%. of Basophils, of Red Blood Cell(RBC) 9.93$\pm$1.38$\times$10/${mu}ell$, of PCV 54.25$\pm$6.25%, of Hb 16.89$\pm$2.43g/㎗, of Tot protein 7.63$\pm$0.6g/㎗, of Cholesterol 87.25$\pm$10.76mg/㎗, of Glucose 109.36$\pm$43.7mg/㎗, of SGOT 85.17$\pm$33IU/$\ell$, of SGPT 102.92$\pm$31.49IU/$\ell$, of BUN 21.00$\pm$5.18mg/㎗, of Creatinine 2.1$\pm$0.43mg/㎗, of ALKP 156$\pm$32.72IU/$\ell$, of Ca 11.28$\pm$1.04mg/㎗, of Na 127.92$\pm$10.04mmo1/$\ell$, of NH3 102.83$\pm$42.25mmo1/$\ell$, of Cl 105.91$\pm$3.45mmo1/$\ell$, respectively. 3. In wapiti, Mean$\pm$SD Values of White Blood Cell(WBC) count were 3450.00$\pm$1040.73/${mu}ell$, of Neutrophils 53.88$\pm$7.42%, of Lymphocytes 36.00$\pm$5.72%, of Monocytes 1.13$\pm$1.05%, of Eosinophils 1.38$\pm$1.93%, of Basophils 7.63$\pm$4.15%, of Red Blood Cell 10.09$\pm$1.3$\times$10/${mu}ell$, of PCV 46.88$\pm$3.33%, of H 15.09$\pm$1.48g/㎗, of Total protein 7.15$\pm$0.73g/㎗, of Cholesterol 78.63$\pm$12.27mg/㎗, of Glucose 75.63$\pm$33.22mg/㎗ , of SGOT 79.25$\pm$18.43IU/$\ell$, of SGPT 36.50$\pm$19.20IU/$\ell$, of BUN 29.63$\pm$4.15mg/㎗, of Creatinine 1.35$\pm$0.21mg/㎗ , of ALKP 283.50$\pm$88.13IU/$\ell$, of Ca 9.78$\pm$0.43mg/㎗ , of Na 120.00$\pm$10.02mmol/$\ell$, of NH3 261.50$\pm$ 103.46mmol/$\ell$, of Cl 92.00$\pm$9.19mmol/$\ell$, respectively.

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Laboratory Markers Indicating Gastrointestinal Involvement of Henoch-Schönlein Purpura in Children

  • Hong, Jeana;Yang, Hye Ran
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.18 no.1
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    • pp.39-47
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    • 2015
  • Purpose: To determine clinically useful biochemical markers reflecting disease activity and/or gastrointestinal (GI) tract involvement in Henoch-$Sch{\ddot{o}}nlein$ purpura (HSP). Methods: A total of 185 children with HSP and 130 controls were included. Laboratory data indicating inflammation, standard coagulation, and activated coagulation were analyzed for the HSP patients, including measurements of the hemoglobin level, white blood cell (WBC) count, absolute neutrophil count (ANC), platelet count, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) level, prothrombin time, activated partial thromboplastin time, and fibrinogen, D-dimer, and fibrin degradation product (FDP) levels. The clinical scores of the skin, joints, abdomen, and kidneys were assessed during the acute and convalescence phases of HSP. Results: The WBC count, ANC, ESR, and CRP, fibrinogen, D-dimer, and FDP levels were significantly higher in the acute phase compared with the convalescent phase of HSP (p<0.05). The total clinical scores were more strongly correlated with the D-dimer (r=0.371, p<0.001) and FDP (r=0.369, p<0.001) levels than with inflammatory markers, such as the WBC count (r=0.241, p=0.001), ANC (r=0.261, p<0.001), and CRP (r=0.260, p<0.001) levels. The patients with GI symptoms had significantly higher ANC (median [interquartile range], 7,138.0 [4,446.4-9,470.0] vs. 5,534.1 [3,263.0-8,153.5], p<0.05) and CRP (0.49 [0.15-1.38] vs. 0.23 [0.01-0.67], p<0.05), D-dimer (2.63 [1.20-4.09] vs. 1.75 [0.62-3.39]), and FDP (7.10 [0.01-13.65] vs. 0.10 [0.01-7.90], p<0.05) levels than those without GI symptoms. Conclusion: D-dimer and FDPs are more strongly associated with disease activity and more consistently reflect GI involvement than inflammatory markers during the acute phase of HSP.

Hemato-Biochemical Indices of Hanwoo Cattle Raised at Different Altitudes (서로 다른 고도에서 사육된 비육한우의 혈액-혈청 지수)

  • Ji, Joong-Ryong;Alam, Md. Rafiqul;Na, Chong-Sam;Kim, Nam-Soo
    • Journal of Veterinary Clinics
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    • v.28 no.2
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    • pp.219-224
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    • 2011
  • The health status of three groups of Hanwoo steers (n=157) aged 2-3 years, raised at three different altitudes (600 m, n=50; 200-400 m, n=58; plane land, n=49) and environment with more or less similar management have been evaluated through hematological, biochemical and globulin examinations in order to determine the optimum environment suitable for raising cattle while at the same time minimizing the risk of disease. Five mL of blood samples from each animal were collected by jugular veinipuncture and 2 mL was transferred to a tube containing EDTA for complete blood count (CBC) and 3 mL in lithium heparin for chemistry screening (CS) and immunoassay. Among the CBC parameters a significantly higher white blood cell count (tWBC), total red blood cell count (tRBC), hemoglobin (Hb) and packed cell volume (PCV) were noticed in the high altitude groups, whereas those were lower in the plane land group. In the CS parameters higher levels of aspartate aminotransferase (AST), creatinine, lactate dehydrogenase (LDH) and total bilirubin (TBL) were found in the plane land group, whereas those were lower in the high altitude groups. The total protein (significantly) and globulins were higher in the 600 m group. The results of this study revealed that the overall health status of the Hanwoo cattle based on the hemato-biochemical indices was superior in the highest altitude and inferior in the plane land group but all the parameters were within the reference range in all the groups. Therefore, for recommendation of a suitable environment at an appropriate altitude for raising cattle there need to be further studied along with the hemato-biochemical parameters; considering, breeding, feeding, management, marketing, waste disposal and other factors.

Red blood cell distribution width is useful in discriminating adult onset Still's disease and sepsis within 24 hours after hospitalization

  • Park, Hee-Jin;Song, Jungsik;Park, Yong-Beom;Lee, Soo-Kon;Lee, Sang-Won
    • The Korean journal of internal medicine
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    • v.33 no.6
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    • pp.1234-1240
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    • 2018
  • Background/Aims: Red blood cell distribution width (RDW) is a value representing the heterogeneity in the size of red blood cell, and it is usually used in distinguishing types of anaemia. Recently, it was reported that it could reflect the burden of inflammation in diverse diseases and their prognosis. Hence, in this study, we investigated whether RDW may contribute to discriminating adult onset Still's disease (AOSD) from sepsis in serious febrile patients within 24 hours after hospitalization. Methods: We reviewed the medical records and enrolled 21 AOSD patients, 27 sepsis patients and 30 matched healthy controls. We collected at least two laboratory results of variables including RDW within 24 hours after hospitalization, and we calculated their mean values. Results: Sepsis patients showed the significantly increased median white blood cell count, compared to AOSD patients ($14,390.0/mm^3$ vs. $12,390.0/mm^3$, p = 0.010). The median RDW in sepsis patients was higher than that in AOSD patients (15.0% vs. 13.3%, p = 0.001), and furthermore, the median RDW in both patient-groups was significantly higher than that in healthy controls. In contrast, the median ferritin level in sepsis patients was lower than that in AOSD patients (544.0 mg/dL vs. 3,756.6 mg/dL, p = 0.001). In multivariate analysis, RDW ${\geq}14.8%$ (odds ratio, 17.549) and ferritin < 2,251.0 mg/dL (odds ratio, 32.414) independently suggested sepsis more than AOSD in patients initially presenting with fever requiring hospitalization. Conclusions: RDW might be a rapid and helpful marker for a differential diagnosis between AOSD from sepsis at an early phase.

The Study on the Ferrokinetics and Acquired Immunity in Repeated Hookworm Infections (구충성빈혈(鉤蟲性貧血)에 관(關)한 연구(硏究))

  • Lee, Mun-Ho;Lee, Pyl-Ung
    • The Korean Journal of Nuclear Medicine
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    • v.1 no.2
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    • pp.61-74
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    • 1967
  • In order to confirm whether acquired immunity or resistance can be developed by the repeated hookworm infections, the 150 mature actively moving filariform ancylostoma duodenale larvae obtained from the severe hookworm anemia patients were orally given to 8 healthy volunteers in three divided doses, 50 in each, at 5 day interval. Also the hematological changes as well as several ferrokinetics using $^{59}Fe$ were done and were compared with 10 controls. The clinical symptoms and signs were checked every day for the first 3 weeks and then twice weekly until the end of the experiment. The appearance of the ova in the stool was examined by the formalin ether method and the ova was counted by the Stoll's method. The following laboratory tests were done: 1) Red blood cell count, venous blood hematocrit(micromethod), hemoglobin count (cyanomethemoglobin method) were checked every 5 to 7 day interval. 2) Plasma iron concentration (Barkan's modified method) was determined every 2 to 3 week interval. 3) Radioisotope studies: a) Ferrokinetics: Huff et al and Bothwell's method were applied. Erythropoietic Index (% of normal)=$\frac{Subject's\;turnover/100ml\;whole\;blood{\times}100}{Average\;normal\;turnover/100ml\;whole\;blood}$ of the gastrointestinal absorption of iron: Radioiron($^{59}Fe$) balance b) Quantitative measurement method was applied. c) Determination of the plasma erythropoietin activity: Fried's method was applied. Following were the results: 1) The serum iron level was lower. The red cell volume was decreased, but with relative increase of plasma volume. 2) The plasma iron disappearance time was accelerated and the plasma iron turnover rate was decreased. The red cell iron turnover rate was markedly increased, while all of the red cell iron concentration, circulating red cell iron. plasma iron pool were decreased. The daily iron pool turnover and red cell renewal rate were increased. 3) The erythropoietic index, erythropoietin activity and intestinal absorption of iron($^{59}Fe$) were markedly increased. 4) The infectivity was $9.8{\pm}1.31%$ which was lower than that observed in the single infection. 5) From these observations, it is concluded that the hookworm anemia is essentially iron deficieny in its origin and some immunity acquisition is possible with repeated infections.

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A Case of Complete Remission of Hairy Cell Leukemia by 2-Chlorodeoxyadenosine (2-Chlorodeoxyadenosine에 의해 완전관해가 유도된 모발상 세포백혈병 1예)

  • Kim, Yong-Gil;Lee, Sang-Jin;Kim, Min-Kyung;Lee, Kyung-Hee;Hyun, Myung-Soo;Cho, Hee-Sun
    • Journal of Yeungnam Medical Science
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    • v.21 no.2
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    • pp.237-241
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    • 2004
  • Hairy cell leukemia (HCL) is an uncommon chronic B-cell lymphoproliferative disorder that is characterized by cytopenia, splenomegaly, and mononuclear cells displaying cytoplasmic projections. We experienced a case of hairy cell leukemia that developed in a 38-year-old man. He showed marked splenomegaly without palpable lymphoadenopathy. A complete blood cell count revealed leukopenia ($3300/{\mu}{\ell}$ with 63% of lymphocyte) and the peripheral blood smear showed abnormal lymphoid cells with cytoplasmic projections. The bone marrow smear revealed abnormal lymphocytes and severe myelofibrosis. Tartrate-resistant acid phosphatase reactivity was strongly positive in the hairy cells. The immunophenotyping results of lymphoid cells were CD5(-), CD10(-), CD19(+), CD25(+), CD103(+), CD20(+), lambda(+). The patient was treated with 2-Chlorodeoxyadenosine at a daily dose of 0.1mg/Kg by a continuous intravenous infusion for 7 days. The patient achieved complete remission.

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Non-B, Non-T Acute Lymphoblastic Leukemia in a Cat

  • Sumin Cha;Hyunwoo Kim;Hyeona Bae;Minjeong Kang;Rankyung Jung;Minji Kim;DoHyeon Yu
    • Journal of Veterinary Clinics
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    • v.40 no.4
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    • pp.298-302
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    • 2023
  • A 7-year-old neutered male, domestic shorthair cat presented anorexia and lethargy. The complete blood cell count revealed severe non-regenerative anemia, lymphocytic leukocytosis, neutropenia, and thrombocytopenia. On the peripheral blood smear examination, medium to large lymphoblastic cells with moderate amounts of basophilic cytoplasm were observed in up to 70% of peripheral leukocytes. Feline leukemia and immunodeficiency viruses were not detected using a commercial diagnostic kit. While splenomegaly and blunt margins of the caudoventral liver were observed in abdominal radiography, changes in the intra-abdominal lymph nodes were not remarkable. Ultimately, flow cytometric immunophenotyping from the peripheral blood revealed a negative for B-cell markers (CD21-/CD79a-) and T-cell markers (CD3-/CD4-/CD5-/CD8-). Based on the hematological examination and the immunophenotyping assay, the cat was diagnosed with non-B, non-T acute lymphoblastic leukemia. Here, we report a rare case of non-B, non-T acute lymphoblastic leukemia to raise awareness and provide information on clinical symptoms and laboratory test and immunophenotyping analysis results.

Relationship between Nutritionally-related Blood Metabolites and Gastrointestinal Parasites in Nguni Goats of South Africa

  • Gwaze, F. Rumosa;Chimonyo, M.;Dzama, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.9
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    • pp.1190-1197
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    • 2010
  • The objective of the study was to determine the relationship between faecal egg counts and nutritionally-related blood metabolites in Nguni goats of South Africa. Body weights, body condition scores (BCS), FAMACHA scores, faecal and blood samples were collected from 96 Nguni castrates. Faecal samples were analysed using the modified McMaster technique for nematodes and the sedimentation method for trematodes. Blood was analysed for packed cell volume (PCV), glucose, cholesterol, total protein, albumin, urea and creatinine. Season had an effect on glucose, globulin, total protein, creatinine, PCV and faecal egg counts (FEC). Globulin, PCV, creatinine and FEC were significantly higher in the wet season compared to the dry season. A quadratic relationship existed between faecal egg count loads and BCS whilst negative linear relationships were observed between faecal egg counts and creatinine, albumin and cholesterol levels of Nguni goats.

A Study on the Relationship between CBC and EEG for Epilepsy Patients (뇌전증 EEG결과와 CBC결과의 관계연구)

  • Jo, Yoon-kyung;Sung, Hyun-Ho;Chae, Kyoung-Min
    • Korean Journal of Clinical Laboratory Science
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    • v.47 no.4
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    • pp.225-229
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    • 2015
  • Epilepsy is a disorder that causes recurring seizures, and the most objective and useful test for detecting epilepsy is the electroencephalogram (EEG). The subjects of this study are 244 patients who received an EEG after being diagnosed with epilepsy at Seoul National University Hospital in 2014, and who have agreed to the purpose of the study. Based on the EEG results, subjects were divided into normal and abnormal groups with 122 subjects in each group, regardless of their gender and age, to investigate the correlation of EEG and complete blood cell count (CBC) test results. The four significant categories that displayed significant correlation between EEG results and CBC hematological measurements in this study were the white blood cell (WBC), red blood cell (RBC), neutrophil, and lymphocyte tests. The WBC (p<0.05) and neutrophil (p<0.01) showed a positive correlation with EEG results, while RBC (p<0.05) and lymphocyte (p<0.01) showed a negative correlation. One of the limitations of this study is that it is lacking the blood test result analysis according to the types of anti-epilepsy medicine. However, the analysis of EEG results by the same disease has significant meaning. Therefore, further studies are needed to statistically analyze more data in the future.

The Effect of Progressive Muscle Relaxation using Biofeedback on Stress Response and Natural Killer Cell in first Clinical Practice of Nursing Students (바이오휘드백을 이용한 점진적 근육이완훈련이 스트레스반응과 면역반응에 미치는 효과)

  • Kim Keum-Soon
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.7 no.1
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    • pp.109-121
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    • 2000
  • Increasingly nursing science is embracing the concepts and methodology derived from psycho-neuroimmunology. It has been previously shown that stress increases and immune function declines in students undergoing examinations. To date, however, no many studies have been reported on stress levels, immune function and interventions in Korean students undergoing their first clinical nursing rotation. It was proposed that nursing students during their first clinical rotation experience increase in stress because of the novelty of the situation and their lack of clinical knowledge. It was also hypothesized that biofeedback and progressive relaxation, methods of self-regulation of involuntary autonomic nervous system responses, would reduce the stress response. The purpose of this study is to test the effectiveness of progressive muscle laxation using biofeedback The effectiveness of the experimental methods was tested by measuring the degree of symptoms of stress (SOS) and the values of ephinephrine, pulse rate, blood pressure and natural killer cells. The subjects of this study were thirty nursing students divided into two groups: experimental group was progressive muscle relaxation group using biofeedback and control group. This study was conducted for 8 weeks of clinical practice. Biofeedback training was done by software developed by J&J company (1-410 form for progressive muscle training). Progressive muscle relaxation training according to Jacobson's Theory was done by messaged word from biofeedback. The data was analyzed using Chronbach' ${\alpha}$ and t-test of the SPSS program and the significance level of statistics was 5%. The results of the study were : 1) The progressive muscle relaxation training using biofeedback was effective for the reduction of symptoms of stress(t=-4.248, p<.001) under clinical practice stress conditions. 2) The progressive muscle relaxation training using biofeedback was not effective for the values of epinephrine(t=-1.294, p=.206). 3) The progressive muscle relaxation training using biofeedback was effective for the reduction of systolic blood pressure (t=-2.757, p=.01). 4) The progressive muscle relaxation training using biofeedback was effective for the reduction of diastolic blood pressure (p=-2.032, 0=.05). 5) The progressive muscle relaxation training using biofeedback was not effective for the reduction of pulse rate(t=-15, p=.988). 6) The progressive muscle relaxation training using biofeedback was effective for the maintenance of natural killer cells (t=2.381, p=02). The first clinical rotation for student nurses is a stressful experience as seen by the rise in the SOS in the control group. Biofeedback using progressive muscle relaxation were effective in preventing the rise of symptoms of stress and the blood pressure means when comparing the pre to post clinical experience, The mean natural killer cell count was depressed in the control group but not significantly different in the experimental groups, It is proposed here that stress via the hypothalamic - pituitary - adrenal axis suppressed the NK cell count whereas the relaxation methods prevented the rise in stress and the resulting immune depression. We recommend relaxation techniques using biofeedback as a health promotion technique to reduce psychological stress. In summary. the progressive muscle relaxation training using biofeedback was effective for the reduction of symptoms of stress under clinical practice stress conditions.

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