• 미생물학회지
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• 제23권1호
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• pp.43-48
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• 1985

철저한 혐기적 방법으로 배양분리한 결과 편성 혐기성세균의 분리율이 높아졌고, 검체당 평균 3.3주가 분리되었다. 혈액배지인 NATB배지와 BA배지에서 비혈액배지인 NAT배지와 GAM배지에서 보다 세균 분리효과가 우수했다. NATB배지에는 혐기성 그람 양성 구균에 좋은 분리효과를 나타내었고, 그람 음성 간균은 15주 분리되었다. 이 중 Bacteroides가 12주로서 가장 많이 존재하는 균종이었다.

• 대한임상검사과학회지
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• 제55권3호
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• pp.159-166
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• 2023

본 연구에서는 진단검사를 수행하기 위해 가장 중요하다고 할 수 있는 채혈 업무에 대한 임상병리사의 현 상황을 알아보고, 이를 통해 채혈 업무에 대한 행위료의 보험 수가 산정 및 상대 가치점수 부여를 통한 임상병리사의 권익을 향상시키고자 하였다. 부산·울산·경남 지역의 병원에서 근무하고 있는 임상병리사를 대상으로 설문조사를 실시하였고, 총 650명의 임상병리사를 대상으로 연구를 수행하였다. 연구결과 1명의 임상병리사가 약 100명의 피검자를 대상으로 채혈 업무를 수행하는 것을 확인할 수 있었다. 채혈 실패 건수는 하루에 1건 이상 실패하는 것을 확인할 수 있었고, 통증 호소 환자 및 이를 통한 민원 발생은 1년간 1건 이상 발생하여 검사자의 부담감이 높은 것을 확인할 수 있었다. 사용한 바늘에 채혈자가 찔리는 경우도 1년간 1건 이상 발생되고 있고, 자상으로 인한 감염관리 진료 및 치료를 받은 경우도 약 15% 발생되고 있음을 확인하였다. 채혈 업무로 인한 근골격계 질환 발생 및 정신적 스트레스를 받는 비율은 절반 이상의 인원이 받는 것을 확인할 수 있었다. 현재 같은 주사침을 이용한 정맥 및 근육 주사와는 달리 채혈 업무에 대한 행위료는 없는 상황이기 때문에, 이러한 연구 결과를 기반으로 채혈 업무에 대한 행위료 산정 및 상대 가치점수 부여를 통해 임상병리사의 안전 및 권익을 향상시킬 수 있을 것으로 사료된다.

• Journal of Preventive Medicine and Public Health
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• 제36권3호
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• pp.209-212
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• 2003

Because of advances of technologies in the field of genmic epidemiology in the recent years, specimen collection, storage and analysis became an essential part of research methodologies. DNA is now being used in epidemiologic studies to evaluate genetic risk factors and specimens other than the fresh whole blood can De used for PCR. Therefore, All nucleated cells, such as buccal swabs and urine specimens, are suitable for DNA analysis. For an unlimited source of genomic DNA, EBV transformation of lymphocytes can be used for immortalization. However, the type of specimen collected in genomic epidemiologic studies will depend on the study where the epidemiologist play a leading role for the design. We also briefly described various finds of analysis for SNP that is an essential part of the genomic epidemiology.

• 임상간호연구
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• 제17권3호
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• pp.399-410
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• 2011

Purpose: This study was conducted to investigate the incidence of blood hemolysis and repeated blood sampling and to identify factors contributing to hemolysis and repeated blood sampling in the emergency department. Methods: A cross-sectional descriptive design was used. Participants were the patients who came to emergency department and are required a blood sampling for electrolyte level. All blood samples were collected by emergency department nurses and determined for hemolysis by experienced laboratory technologists. Data were analyzed using $x^2$-test, Fisher's exact test, Mann-Whitney u test and Binary Logistic Regression to determine significant differences. Results: A total of 402 valid samples were collected. Of these, 30 blood samples (7.5%) were found to be hemolyzed and 9 (2.2%) to be recollected. Statistically significant factors affecting on hemolysis and repeated blood sampling included the time of bloods sampling (night), the time of tourniquet application, and too-fast blood draw into the test tube. Conclusion: We recommend that nurses who take the blood sampling to consider the findings of the study and take the related factors into account as they set up the standardized care protocol in order for nursing quality improvement.

• Parasites, Hosts and Diseases
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• 제47권2호
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• pp.179-183
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• 2009

Prevalence rates reported for malaria in pregnancy in Nigeria vary considerably. The accuracy of results of malaria diagnosis is dependent on training, experience, and motivation of the microscopist as well as the laboratory facility available. Results of training programmes on malaria microscopy have shown low levels of sensitivity and specificity of those involved in malaria diagnosis routinely and for research. This study was done to ascertain the true prevalence of malaria in pregnancy in Lagos, South-West Nigeria. A total of 1,084 pregnant women were recruited into this study. Blood smears stained with Giemsa were used for malaria diagnosis by light microscopy. Malaria infection during pregnancy presents mostly as asymptomatic infection. The prevalence of malaria in this population was 7.7% (95% confidence interval; 6.2-9.4%). Factors identified to increase the risk of malaria infection include young maternal age (<20 years), and gravidity (primigravida). In conclusion, this study exposes the over-diagnosis of malaria in pregnancy and the need for training and retraining of laboratory staffs as well as establishing the malaria diagnosis quality assurance programme to ensure the accuracy of malaria microscopy results at all levels.

• 대한임상검사과학회지
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• 제38권3호
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• pp.147-151
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• 2006

Although various automated CBC analyzers with different WBC analytical principles were consequently introduced to clinical laboratory, the specific information concerning the suitability or unsuitability of aging samples is scarce. For this reason, we studied the effect of storage duration and temperature on CBC parameter in SE-9000 (SYSMEX Medical Electronics Co., Ltd., Kobe, Japan), automated CBC analyzer. We tested 32 K3-EDTA specimens with SE-9000 during 72 hours. Specimens were kept at room temperature (RT) and refrigerated and were analyzed at 0 hr, 4 hr, 8 hr, 24 hr, 48 hr and 72 hr after the collection of the specimens. The percentage changes from initial value for each parameters were calculated. Among the CBC parameters, hemoglobin, red blood cell count, mean corpuscular hemoglobin and platelets were stable for the study period at both temperatures. The mean corpuscular volume (MCV), hematocrit (Hct) and red cell distribution (RDW) increased and the mean corpuscular hemoglobin concentration (MCHC) decreased over time at room temperature. These parameters were stable when refrigerated. The leukocyte count was stable during 72hr at RT and when refrigerated. At room temperature, the relative percentages of neutrophils tend to increase, whereas those of lymphocyte and monocytes tend to decrease after 48 hours. When refrigerated, those of neutrophils and monocytes tend to increase, whereas those of lymphocytes tend to decreased over time. CBC parameters of refrigerated specimen were reliable for 72 hr for the exception of differential count from 24 hr but many CBC parameters, such as MCV, Hct, MCHC, RDW and differential count of leukocyte of blood stored at room temperature for 24 hr were unreliable.

• 대한간호학회지
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• 제42권3호
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• pp.443-451
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• 2012

Purpose: This study was done to compare the rates of hemolysis and repeated sampling in blood samples obtained by a syringe needle versus a vacuum tube needle. Methods: A randomized, prospective study was used to evaluate the differences between the two blood sampling methods. The study group consisted of patients seen in the emergency department (ED) for blood sampling to determine electrolyte level. ED patients were randomly assigned to either the syringe group or the vacuum tube group. All blood samples were collected by experienced ED nurses and hemolysis was determined by experienced laboratory technologists. Data were analyzed using Fisher's exact test and binary logistic regression. Results: One hundred forty-five valid samples were collected (74 in the syringe group versus 71 in the vacuum tube group). 5 of 74 (6.8%) blood samples in the syringe group and 8 of 71 (11.3%) in the vacuum tube group hemolyzed. Repeated blood sampling occurred for 2 of 74 (2.7%) and 3 of 71 (4.2%) in each group respectively. There were no significant differences in rates of hemolysis and repeated sampling between two groups (B=1.97, p=.204; B=2.36, p=.345). Conclusion: Venipuncture with syringe needles can be recommended for ED nurses to obtain blood samples.

• Anatomy and Cell Biology
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• 제56권1호
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• pp.25-31
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• 2023

Johannes Nathanael Lieberkühn was a prodigious anatomist whose meticulous experiments and precise detailing helped in comprehending the microscopic anatomy of digestive system during early part of eighteenth century. Notably, his inventions in the field of microscopy aptly complemented his quest for anatomical knowledge at microscopic level. He designed a reflector (Lieberkühn reflector) which enhanced the amount of focussed light leading to bright illumination of tissue specimen. He invented the solar microscope which provided excellent resolution of minute anatomical details. Lieberkühn discovered the digestive juice secreting tubular glands (glands of Lieberkühn) present at the base of intestinal villi producing epithelial invaginations (crypts of Lieberkühn). He also described the intricate juxtaposition of blood vessels in relation to a single intestinal villi. Moreover, through empirically designed experimental set up, Lieberkühn was able to demonstrate the flow of lymph from intestinal villi to collecting lymphatic vessels. Also, his grandiose collection of laboratory specimens involving vascular anatomy are a testimony of his untiring efforts in academia. His contributions were seminal in comprehending the anatomy of digestive system and paved the way for future revelations. His work unveiled the enormous scope of microanatomy in medical science and catalysed the advent of histological staining methods a century later.

• 대한영상의학회지
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• 제85권2호
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• pp.456-462
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• 2024

원발성 알도스테론증은 자율신경계에 의한 알도스테론 분비조절의 장애로 고혈압 및 저칼륨혈증과 관련이 있다. 원발성 알도스테론증에서 편측성을 결정하는 것이 매우 중요한 이유는 그에 따라 치료 방법이 달라지기 때문이다. 부신정맥채혈술은 원발성 알도스테론증에서 편측성을 평가하는 가장 신뢰성 있는 방법으로 알려져 있다. 부신정맥채혈술은 부신 정맥이 크기가 매우 작으며 그 해부학적 위치가 다양하기 때문에 기술적으로 어려운 시술이다. 따라서 성공적인 시술을 위해서는 해부학적 변이를 잘 이해하고 시술 전 영상 검사를 면밀히 검토하는 것이 중요하다. 부신정맥채혈술 중에 발견된 세 가지 해부학적 변이를 보고하고자 한다.

• 대한영상의학회지
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• 제84권2호
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• pp.386-397
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• 2023

일차성고알도스테론혈증은 고혈압의 원인 중 높은 비중을 차지하는 질병이다. 부신정맥채혈술은 일차성고알도스테론혈증의 원인을 감별하여 최종적인 치료방침을 결정하는 데 있어 필수적인 검사이다. 부신정맥채혈술의 성공 여부는 각 부신정맥에서 채혈한 샘플의 혈중 코티솔 농도와 말초혈관에서 채혈한 샘플의 혈중 코티솔 농도의 비를 측정하여 판단한다. 하대정맥으로 바로 연결되는 오른부신정맥에서 시술의 실패율이 더 높게 보고되며 상대적으로 왼부신정맥의 시술 실패율은 낮지만 드물게 보고된다. 본 임상화보에서는 왼부신정맥 부신정맥채혈술의 실패 사례를 소개하고 분석하여 최적의 부신정맥채혈술을 위한 고려사항에 대해 고찰하고자 한다.